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Home Species Homo sapiens
Breast Cancer

Basic Information

Homo sapiens / Breast Cancer
Species: Human
Category: Human Cancer Studies
Description: Breast cancer remains a worldwide public health dilemma and is currently the most common tumour in the globe. It is life-threatening disease in females and the leading cause of mortality among women population. This type of cancer seems to be highly heterogeneous and has potentially aggressive and complex biological features. Awareness of breast cancer, public attentiveness, and advancement in breast imaging has made a positive impact on recognition and screening of breast cancer.

Gene Summary

mRNA

ICG00153-14
ACTB RPS23 HUWE1 EEF1A1 SF3A1
ICG00153-54
GAPDH CCSER2 PCBP1
ICG00153-55
18S ACTB GAPDH HSPCB

Studies (3)

ICG00153-14

Breast Cancer
Cancer Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACTB Actin
Tumor Tissues
Normal Epithelium
 NM_001101
F: CTGGAACGGTGAAGGTGACA
R: AAGGGACTTCCTGTAACAACGCA
 140 64 SYBR
RPS23 Ribosomal Protein S23
Tumor Tissues
Normal Epithelium
 NM_001025
F: CGGTGCTTCTCTCTTTCGCT
R: ATGCCACTTCTGGTCTCGT
 110 64 SYBR
HUWE1 Ubiquilin
Tumor Tissues
Normal Epithelium
 NM_031407
F: CAGAGTTGGACAGAGTGAAA
R: TACACAGAGAGAGGAGGACA
 137 64 SYBR
EEF1A1 Elongation Factor
Tumor Tissues
Normal Epithelium
 NM_001402
F: TGAAAACTACCCCTAAAAGCCA
R: TATCCAAGACCCAGGCATACT
 208 64 SYBR
SF3A1 Splicing Factor
Tumor Tissues
Normal Epithelium
 NM_005877
F: AAGGGTCCAGTGTCCATCAAAGT
R: GCCATGTTGTAGTAAGCCAGTGAG
 224 64 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Alexander G Tonevitsky
Email: d.maltseva@bioclinicum.com
Institution: SRC Bioclinicum, Moscow, Russia

Reference

High-throughput identification of reference genes for research and clinical RT-qPCR analysis of breast cancer samples.
Maltseva DV, Khaustova NA, Fedotov NN, Matveeva EO, Lebedev AE, Shkurnikov MU, Galatenko VV, Schumacher U, Tonevitsky AG.
Journal of clinical bioinformatics. 2013-07-22

ICG00153-54

Breast Cancer
Multiple Passages
Cell Types
Cancer Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Mcf-7 Atcc Cell Line
Mcf-7 Htb-22 Cell Line
Nutrient Stress
 NM_002046
F: GACAGTCAGCCGCATCTTCT
R: TTAAAAGCAGCCCTGGTGAC
 127 58 SYBR
CCSER2 Coiled Coil Serine Rich Protein
Mcf-7 Atcc Cell Line
Mcf-7 Htb-22 Cell Line
Nutrient Stress
 NM_018999
F: GACAGGAGCATTACCACCTCAG
R: CTTCTGAGCCTGGAAAAAGGGC
 143 58 SYBR
PCBP1 Poly(Rc) Binding Protein
Mcf-7 Atcc Cell Line
Mcf-7 Htb-22 Cell Line
Nutrient Stress
 NM_006196
F: TGATCATCGACAAGCTGGAG
R: TCTTTGATCTTACACCCGCC
 145 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Inese Cakstina
Email: inese.cakstina@rsu.lv
Institution: Laboratory of Molecular Genetics, Institute of Oncology, Riga Stradiņš University, Dzirciema street 16, Riga LV-1007, Latvia

Reference

Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line.
Jain N, Nitisa D, Pirsko V, Cakstina I.
BMC molecular and cell biology. 2020-09-25

ICG00153-55

Breast Cancer
Transient Transfection
No-Transient Transfection
Cell Types
Cancer Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
18S 18S Ribosomal RNA
Mcf-7 Cell Line
T47D Cell Line
Hcc1500 Cell Line
Bt474 Cell Line
Hcc1806 Cell Line
Sum149Pt Cell Line
Hcc1937 Cell Line
Mda-Mb-231 Cell Line
Skbr3 Cell Line
Mcf-10A Cell Line
 NR_003286
F: GGATGTAAAGGATGGAAAATACA
R: TCCAGGTCTTCACGGAGCTTGTT
 72 61 SYBR
ACTB Actin
Basal Immunoprofile
Hcc1806 Cell Line
Sum149Pt Cell Line
Hcc1937 Cell Line
Mda-Mb-231 Cell Line
 NM_001101
F: TGACGTGGACATCCGCAAAG
R: CTGGAAGGTGGACAGCGAGG
 205 61 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Basal Immunoprofile
Hcc1806 Cell Line
Sum149Pt Cell Line
Hcc1937 Cell Line
Mda-Mb-231 Cell Line
 NM_002046
F: GACAGTCAGCCGCATCTTCT
R: TTAAAAGCAGCCCTGGTGAC
 127 61 SYBR
HSPCB Heat Shock Protein
Er+ Immunoprofile
Mcf-7 Cell Line
T47D Cell Line
Hcc1500 Cell Line
Bt474 Cell Line
 NM_007355
F: TCTGGGTATCGGAAAGCAAGCC
R: GTGCACTTCCTCAGGCATCTTG
 80 61 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Ya-Ping Zhang
Email: zhangyp@mail.kiz.ac.cn
Institution: Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, China

Reference

Identification of valid reference genes for the normalization of RT-qPCR expression studies in human breast cancer cell lines treated with and without transient transfection.
Liu LL, Zhao H, Ma TF, Ge F, Chen CS, Zhang YP.
PloS one. 2015-01-24