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Home Species Homo sapiens
Fibroblast Model

Basic Information

Homo sapiens / Fibroblast Model
Species: Human
Category: Human Biological Model
Description: Fibroblasts are important cells for the support of homeostatic tissue function. In inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease, fibroblasts take on different roles (a) as inflammatory cells themselves and (b) in recruiting leukocytes, driving angiogenesis, and enabling chronic inflammation in tissues. Besides, Fibroblasts include diverse cell types based on developmental origin, anatomic location, and function.

Gene Summary

mRNA

ICG00153-79
ACTB TUBB1 GAPDH
ICG00153-103
GAPDH SDHA TBP HPRT1 B2M

Studies (2)

ICG00153-103

Dermal Fibroblast Model
Uv Irradiation Stress
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Normoxia Condition
 NM 002046
F: TCCACTGGCGTCTTCACC
R: GGCAGAGATGATGACCCTTTT
 78 60 SYBR
SDHA Succinate Dehydrogenase Complex
Normoxia Condition
 NM 004168
F: GGAAGCATAAGAACATCGGAACTG
R: CTGATTTTCCCACAACCTTCTTGC
 110 60 SYBR
TBP TATA-binding Protein
Hypoxia Condation
Exposed To 18 J/Cm2 Uva
 NM 003194
F: GGAATCCCTATCTTTAGTCCAATG
R: GACTATTGGTGTTCTGAATAGGC
 76 60 SYBR
HPRT1 Hypoxanthine Phosphoribosyltransferase
Hypoxia Condation
Exposed To 18 J/Cm2 Uva
 NM 000194
F: TGACACTGGCAAAACAATGCA
R: GGTCCTTTTCACCAGCAAGCT
 94 60 SYBR
B2M Microglobulin
Hypoxia Condation
Exposed To 18 J/Cm2 Uva
 NM 004048
F: CATTCCTGAAGCTGACAGCATTC
R: TGCTGGATGACGTGAGTAAACC
 136 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: E. Damiani
Email: e.damiani@univpm.it
Institution: Dipartimento Scienze della Vita e dell’Ambiente, Via Brecce Bianche, Università Politecnica delle Marche, I-60131 Ancona, Italy

Reference

Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: is β-actin a reliable normalizer for photoaging studies?
Brugè F, Venditti E, Tiano L, Littarru GP, Damiani E.
Journal of biotechnology. 2011-09-22

ICG00153-79

Skin Fibroblast Model
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACTB Actin
Dermal Fibroblasts
Ultraviolet-B Irradiation
 NM_001101.3
F: GGCGGCAACACCATGTACCCT
R: AGGGGCCGGACTCGTCATACT
 205 62 SYBR
TUBB1 Tubulin
Dermal Fibroblasts
Ultraviolet-B Irradiation
 30773.2
F: CCTTGCAGCTGGAGAGAATC
R: CTGTCGGGTTGAAAGAGAGC
 145 59 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Dermal Fibroblasts
Ultraviolet-B Irradiation
 NM_002046.3
F: ACAGTCAGCCGCATCTTCTT
R: GTTAAAAGCAGCCCTGGTGA
 127 57 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Baoxi Wang
Email: wangbx1583@263.com
Institution: Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Dermatology, Nanjing, PR China

Reference

Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCR.
Li L, Yan Y, Xu H, Qu T, Wang B.
BMC molecular biology. 2011-02-17