Basic Information

Homo sapiens / Gallbladder Cancer
Species: Human
Category: Human Cancer Studies
Description: Gallbladder Carcinoma (GBCA) is the most common biliary tract malignancy. As the disease is often diagnosed clinically in an advanced stage, the survival rates are dismal. Recent advances in cross-sectional imaging techniques have increased the preoperative detection rates of GBCA. Gallbladder wall thickening is the most common finding of early GBCA. However, it is also the most common presentation of benign biliary tract disorders. In advanced GBCA, the key issue is the local tumor stage and presence of distant metastasis.

Gene Summary

mRNA

ICG00153-51

Studies (1)

ICG00153-51

Gallbladder Carcinoma
Cancer Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Gallbladder Carcinoma Tissues
Without Treatment
-
F: GACAGTCAGCCGCATCTTCT
R: TTAAAAGCAGCCCTGGTGAC
127 55 SYBR
PBGD Hydroxymethylbilane Synthase
Gallbladder Carcinoma Tissues
Without Treatment
-
F: AGTGTGGTGGGAACCAGC
R: CAGGATGATGGCACTGAACTC
144 55 SYBR
ALAS1 Gap Junction Protein
Gallbladder Carcinoma Tissues
Without Treatment
-
F: GGCAGCACAGATGAATCAGA
R: CCTCCATCGGTTTTCACACT
150 55 SYBR
PPIA Peptidylprolyl Isomerase
Paired Normal Tissues
-
F: AGACAAGGTCCCAAAGAC
R: ACCACCCTGACACATAAA
118 55 SYBR
PUM1 Pumilio RNA Binding Family Member
Paired Normal Tissues
-
F: CAGGCTGCCTACCAACTCAT
R: GTTCCCGAACCATCTCATTC
211 55 SYBR
TBP TATA-binding Protein
Paired Normal Tissues
-
F: TGCACAGGAGCCAAGAGTGAA
R: CACATCACAGCTCCCCACCA
132 55 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Maria L. Brandi
Email: marialuisa.brandi@unifi.it
Institution: Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Study of Florence, Viale Pieraccini 6, 50139 Florence, Italy

Reference

Identification of suitable reference genes for investigating gene expression in human gallbladder carcinoma using reverse transcription quantitative polymerase chain reaction.
Yu S, Yang Q, Yang JH, Du Z, Zhang G.
Molecular medicine reports. 2014-11-27