Basic Information

Homo sapiens / Heart Disease
Species: Human
Category: Human Non-cancer Disease Studies
Description: Heart failure is considered, deadly, incapacitating and a heavy disorder worldwide. The occurrence of HF in developed countries has extended epidemic proportions increasingly. In spite of remarkable therapeutic developments, the prediction of patients with Heart Failure (HF) is weak, and present therapeutic attitudes do not report the fundamental problem of the cardiac tissue loss. Innovative therapies are required to reduce mortality and limit or abolish the necessity for cardiac transplantation.

Gene Summary

mRNA

ICG00153-59
ICG00153-13

Studies (2)

ICG00153-13

Heart Disease
Myocardium
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
SRP14 Signal Recognition Particle
Left Ventricular Free Wall
Myocardium
Explanted Failed Hearts
Non-Failed Heart Tissue
Case Control Study
NM_003134
F: CAGATGGCTTATTCAAACCTCCT
R: ATGCCCTTTACTGTGCTGCT
181 56 SYBR
TPT1 Tumor Protein, Translationally Controlled
Left Ventricular Free Wall
Myocardium
Explanted Failed Hearts
Non-Failed Heart Tissue
Case Control Study
NM_003295
F: AAATGTTAACAAATGTGGCAATTAT
R: AACAATGCCTCCACTCCAAA
164 56 SYBR
EEF1A1 Elongation Factor
Left Ventricular Free Wall
Myocardium
Explanted Failed Hearts
Non-Failed Heart Tissue
Case Control Study
NM_015905
F: CTTTGGGTCGCTTTGCTGTT
R: CCGTTCTTCCACCACTGATT
183 56 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Vicky A Cameron
Email: vicky.cameron@otago.ac.nz
Institution: Christchurch Cardioendocrine Research Group, Department of Medicine, University of Otago-Christchurch, PO Box 4345, Christchurch 8014, New Zealand

Reference

Genomic selection of reference genes for real-time PCR in human myocardium.
Pilbrow AP, Ellmers LJ, Black MA, Moravec CS, Sweet WE, Troughton RW, Richards AM, Frampton CM, Cameron VA.
BMC medical genomics. 2008-12-29

ICG00153-59

Heart Disease
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
RPL5 Ribosomal Protein L5
Right Ventricle
NM_000969
F: TCGTATAGCAGCATGAGCTTTC
R: TGTTGCAGATTACATGCGCT
136 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Right Ventricle
Left Ventricle
Heart Failure Sample
Normal Heart Sample
NM_002046
F: TTAAAAGCAGCCCTGGTGAC
R: CTCTGCTCCTCCTGTTCGAC
144 60 SYBR
PSMB4 Proteasome
Right Ventricle
NM_002796
F: GCATTATGCGAGTCAACAAC
R: ACAAAGGGTTCATCTTCGAG
198 60 SYBR
REEP5 Receptor Accessory Protein
Left Ventricle
NM_005669
F: CATCGCTCTTGGTGTCATC
R: ATACACTACCCAGTAGGTCA
172 60 SYBR
RAB7A Ras Homolog Family Member
Left Ventricle
NM_004637
F: CTGAAGGTTATCATCCTGGG
R: GTCCCATATCTGCATTGTGA
165 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Jiangping Song
Email: fwsongjiangping@126.com
Institution: State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing 100037, China

Reference

Selection of reference genes for gene expression studies in heart failure for left and right ventricles.
Li M, Rao M, Chen K, Zhou J, Song J.
Gene. 2017-04-05