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Home Species Homo sapiens
Immune System Model

Basic Information

Homo sapiens / Immune System Model
Species: Human
Category: Human Biological Model
Description: The immune system was identified as a protective factor during infectious diseases over a century ago. Current definitions and textbook information are still largely influenced by these early observations, and the immune system is commonly presented as a defence machinery. In fact, the immune system is integral part of fundamental physiological processes such as development, reproduction and wound healing, and a close crosstalk between the immune system and other body systems such as metabolism, the central nervous system and the cardiovascular system is evident.

Gene Summary

mRNA

ICG00153-78
RPL13A B2M
ICG00153-9
ACTB RPL37A
ICG00153-101
TBP SDHA ACTB
ICG00153-77
B2M IPO-8 GAPDH

Studies (4)

ICG00153-9

Immune System Model
Cell Types
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACTB Actin
Thp-1 Monocyte Differentiation
Macrophage Stage
Pma Inducement
Thp-1 Monocyte Cell Line
 NM_001101
F: ATTGCCGACAGGATGCAGAA
R: GCTGATCCACATCTGCTGGAA
 150 60 SYBR
RPL37A Ribosomal Protein L37
Thp-1 Monocyte Differentiation
Macrophage Stage
Pma Inducement
Thp-1 Monocyte Cell Line
 NM_000998.4
F: ATTGAAATCAGCCAGCACGC
R: AGGAACCACAGTGCCAGATCC
 94 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Stefan Lorkowski
Email: stefan.lorkowski@uni-jena.de
Institution: Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Str. 25, 07743 Jena, Germany

Reference

Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages.
Maess MB, Sendelbach S, Lorkowski S.
BMC molecular biology. 2010-12-01

ICG00153-77

Immune System Model
Natural Killer Cell
Cell Types
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
B2M Microglobulin
Nk-94 Cell Line
Stimulated With Il-2
Stimulated With Tnf
Non-Activated Cells
 -
F: TTAGCTGTGCTCGCGCTACTCT
R: TGGTTCACACGGCAGGCATACT
 294 59 SYBR
IPO-8 Importin
Nk-94 Cell Line
Stimulated With Il-2
Stimulated With Tnf
Non-Activated Cells
 -
F: TTGGAAGAAACCGCGCTTGAGG
R: ACCAGGCTGCATCTCGACTCTG
 118 59 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Nk-94 Cell Line
Stimulated With Il-2
Stimulated With Tnf
Non-Activated Cells
 -
F: CTGTTCGACAGTCAGCCGCATC
R: GCGCCCAATACGACCAAATCCG
 110 59 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Lucyna Kaszubowska
Email: lkras@gumed.edu.pl
Institution: Department of Histology, Medical University of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland

Reference

Optimal reference genes for qPCR in resting and activated human NK cells--Flow cytometric data correspond to qPCR gene expression analysis.
Kaszubowska L, Wierzbicki PM, Karsznia S, Damska M, Ślebioda TJ, Foerster J, Kmieć Z.
Journal of immunological methods. 2015-04-22

ICG00153-78

Immune System Model
T Lymphocyte
Mesenchymal Cell Co-Cultured
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPL13A Ribosomal Protein L13
Cultured T Lymphocytes
Mesenchymal Co-culture
Bone Marrow Co-culture
Adipose Tissue
Wharton's Jelly Co-culture
Placenta Co-culture
 NM_012423.2
F: CGAGGTTGGCTGGAAGTACC
R: CTTCTCGGCCTGTTTCCGTAG
 121 55 SYBR
B2M Microglobulin
Cultured T Lymphocytes
Mesenchymal Co-Culture
Bone Marrow Co-Culture
Adipose Tissue
Wharton'S Jelly Co-Culture
Placenta Co-Culture
 NM_004048.2
F: CTATCCAGCGTACTCCAAAG
R: GAAAGACCAGTCCTTGCTGA
 188 55 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: YIMIN WANG
Email: yiminwang@hotmail.com
Institution: The Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033

Reference

Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells.
Li X, Yang Q, Bai J, Xuan Y, Wang Y.
Molecular medicine reports. 2015-09-30

ICG00153-101

Immune System Model
Neutrophil
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
TBP TATA-binding Protein
Neutrophils
Untreated Blood Leukocytes
 NM_003194
F: Roche-Diagnostics: 101145
R: Roche-Diagnostics: 101145
 87 60 SYBR
SDHA Succinate Dehydrogenase Complex
Neutrophils
Untreated Blood Leukocytes
 NM_004168
F: Roche-Diagnostics: 102136
R: Roche-Diagnostics: 102136
 114 60 SYBR
ACTB Actin
Neutrophils
 NM_001101
F: Roche-Diagnostics: 101125
R: Roche-Diagnostics: 101125
 91 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Simone Kreth
Email: simone.kreth@med.uni-muenchen.de
Institution: Department of Anesthesiology, Ludwig-Maximilians-University Munich, Germany

Reference

Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils.
Ledderose C, Heyn J, Limbeck E, Kreth S.
BMC research notes. 2011-10-20