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Home Species Homo sapiens
Intestinal Disease

Basic Information

Homo sapiens / Intestinal Disease
Species: Human
Category: Human Non-cancer Disease Studies
Description: Gastrointestinal diseases comprise a group of chronic, inflammatory diseases of the gastrointestinal (GI) tract, that are characterized, clinically, by symptoms related to the dysfunction of the involved segment(s) of the GI tract, and histologically, by dense eosinophilic inflammation, in the absence of an identifiable secondary cause. The potential pathogenic mechanism of gut dysbacteriosis associated with intestinal diseases includes the alteration of composition of gut microbiota as well as the gut microbiota-derived signalling molecules. Currently, faecal microbial transplantation, dietary interventions, use of probiotics, prebiotics and drugs are the major therapeutic tools utilized to impact dysbacteriosis and associated intestinal diseases.

Gene Summary

MicroRNA

ICG00153-70
hsa-miR-27a-3p hsa-miR-191-5p hsa-miR-16-5p

mRNA

ICG00153-71
PGK1

Studies (2)

ICG00153-70

Inflammatory Bowel Disease
Pediatric Patients
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
hsa-miR-27a-3p MiRNA
Crohn' S Disease
Small Bowel
Ulcerative Colitis
 MIMAT0004501
F: AGGGCUUAGCUGCUUGUGAGCA
R: Please contact with the researchers for details.
 - 60 TaqMan
hsa-miR-191-5p MiRNA
Crohn' S Disease
Small Bowel
Ulcerative Colitis
 MIMAT0000440
F: CAACGGAAUCCCAAAAGCAGCUG
R: Please contact with the researchers for details.
 - 60 TaqMan
hsa-miR-16-5p MiRNA
Ulcerative Colitis
 MIMAT0000069
F: UAGCAGCACGUAAAUAUUGGCG
R: Please contact with the researchers for details.
 - 60 TaqMan

Molecular Types:

MicroRNA

Evaluation Methods

NormFinder

Contact

Name: Catherine J. Hunter
Email: Chunter@luriechildrens.org
Institution: C. J. Hunter, Ann and Robert H. Lurie Children’s Hospital of Chicago, Div. of Pediatric Surgery, 225 E. Chicago Ave., Box 63, Chicago IL 60611

Reference

Identification of suitable reference microRNA for qPCR analysis in pediatric inflammatory bowel disease.
Buonpane C, Ares G, Benyamen B, Yuan C, Hunter CJ.
Physiological genomics. 2019-04-12

ICG00153-71

Probiotic Bacteria Infection
Pathogenic Bacteria Infection
Cell Types
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
PGK1 Phosphoglycerate Kinase
Colonic Epithelium
Ht-29 Cell Line
Probiotic Bacteria Treatment
Pathogenic Bacteria Treatment
 NM_000291
F: GAGATGATTATTGGTGGTGGAA
R: AGTCAACAGGCAAGGTAATC
 - 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Nikolai Scherbak
Email: nikolai.scherbak@oru.se
Institution: School of Science and Technology, O ̈ rebro University, O ̈ rebro, Sweden

Reference

Reference gene selection for qPCR is dependent on cell type rather than treatment in colonic and vaginal human epithelial cell lines.
Jacobsen AV, Yemaneab BT, Jass J, Scherbak N.
PloS one. 2014-12-19