ICG00172 Plutella xylostella - Internal Control Genes Database (ICG)

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Plutella xylostella

Accession: ICG00172

Latin Name: Plutella xylostella

Common Name: Diamondback moth

Taxonomy ID: 51655

Description: Plutella xylostella (L.) is a highly destructive pest of cruciferous crops worldwide. It has developed resistance to a wide array of insecticides. Its genome is the very first genome documenting an insect pest of agricultural importance. Moreover, it has become an emerging insect model to study insect-plant interactions.

ICG00172-1

Developmental Stages

Tissues

Abiotic Stresses

Temperature Conditions

Photoperiod Conditions

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
RPS13	Ribosomal Protein S13	Photoperiod Effect Insecticide Treatment Egg All Four Larval Instars Pupae Adult	AY174891	F: TCAGGCTTATTCTCGTCG R: GCTGTGCTGGATTCGTAC	100	60	SYBR
RPS23	Ribosomal Protein S23	Egg All Four Larval Instars Pupae Adult Insecticide Treatment	AY174891	F: ATGGGCTGACAAGGATTAC R: TGCGGATGGCAGAGTT	140	60	SYBR
EF1	Elongation Factor	Strain Insecticide Treatment Photoperiod Effect Mechanical Damage Egg All Four Larval Instars Pupae Adult	EF417849	F: GCCTCCCTACAGCGAATC R: CCTTGAACCAGGGCATCT	161	60	SYBR
RPL32	Ribosomal Protein L32	Strain Insecticide Treatment Photoperiod Effect Mechanical Damage High Temperature Condition Low Temperature Condition Egg All Four Larval Instars Pupae Adult	AB180441	F: CCAATTTACCGCCCTACC R: TACCCTGTTGTCAATACCTCT	120	60	SYBR
ACTB	Actin	Strain High Temperature Condition Low Temperature Condition Normal Temperature Condition	AB282645	F: GCGACTTGACCGACTACCT R: GCCGCAAGATTCCATACCC	272	60	SYBR
GAPDH	Glyceraldehyde-3-Phosphate Dehydrogenase	Strain High Temperature Condition Low Temperature Condition Mechanical Damage	AJ489521	F: GCCACCACTGCCACTC R: CGGGACGGGAACACG	177	60	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Youjun Zhang

Email: zhangyoujun@caas.cn

Institution: Institute of Pesticide Science, Hunan Agricultural University, Changsha 410128, China

Reference

ICG00172-2

Abiotic Stresses

Developmental Stages

Strains

Tissues

Insecticide Treatments

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
RPS4	Ribosomal Protein S4	Egg 1St Larval Instar 2Nd Larval Instar 3Rd Larval Instar 4Th Larval Instar Pupae Male Female	Px005732	F: ATGGATGTTGTGTCGATTGAAAAGA R: GAGTGATGCGGTGGATGGTGA	88	-	SYBR
RPL8	Ribosomal Protein L8	Susceptible Strain (Ss) Chlorpyrifos-Resistant Strains (Crs) Fipronil-Resistant Strains (Frs) Head Midgut Malpighian Tubule Fat Body Epidermis	Px009839	F: CGGTCGTGCCTACCACAAATACA R: CGTGAGGATGCTCCACAGGGT	89	-	SYBR
EF1-a	Elongation Factor	Insecticide Treatment	Px015250	F: GCCCTCCAAGCCTCTGTGTGT R: ACCTGAGGGGTCCTTGAAGTTGA	130	-	SYBR
miR11	MiRNA	Brain Midgut Silk Gland Malpighian Tubule Fat Body Hemolymph Instar Larvae Adult	-	F: ACACTCCAGCTGGGCATCACAGTCAGAG R: ACACTCCAGCTGGGCATCACAGTCAGAG	-	58	TaqMan

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Minsheng You

Email: zhangyoujun@caas.cn

Institution: Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Reference

ICG00172-3

Developmental Stages

Tissues

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
miR11	MiRNA	Brain Midgut Silk Gland Malpighian Tubule Fat Body Hemolymph Instar Larvae Adult	-	F: ACACTCCAGCTGGGCATCACAGTCAGAG R: ACACTCCAGCTGGGCATCACAGTCAGAG	-	58	TaqMan

Molecular Types:

MicroRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Minsheng You

Email: msyou@fafu.edu.cn

Institution: State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China

Basic Information

Gene Summary

MicroRNA

mRNA

Studies (3)

ICG00172-1

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00172-2

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00172-3

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00172-2	RPS4 RPL8 EF1-a
ICG00172-1	RPS13 RPS23 EF1 RPL32 ACTB GAPDH