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Ganoderma lucidum

Basic Information

Ganoderma lucidum
Accession: ICG00191
Latin Name: Ganoderma lucidum
Common Name: Ling zhi
Taxonomy ID: 5315
Description: Ganoderma lucidum, a white rot basidiomycete belonging to the order Polyporales, is one of the most well-known medicinal mushrooms in the world. G. lucidum has been used as a remedy for thousands years in East Asia. This species is documented as LingZhi in Chinese Pharmacopoeia and is included in the American Herbal Pharmacopoeia and Therapeutic Compendium.

Gene Summary

mRNA

ICG00191-3
PP2A UCE2
ICG00191-4
ACTIN CYP TIF UBQ1 UCE2
ICG00191-1
PP2A RPL4 BTU
ICG00191-2
RPS18 EF1A RPL4 ACTIN UCE2

Studies (4)

ICG00191-1

Hosts
Growth Phases

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
PP2A Protein Phosphatase
During Fermentation
 -
F: CACGCTTATCATATCCACTCCA
R: GGCTTGATGTCCACGATGTT
 131 60 SYBR
RPL4 Ribosomal Protein L4
Primordia
Stipes Of Fruiting Bodies
Pilei Of Fruiting Bodies
 -
F: GTCAACAAGGGCGTTCTCTT
R: ACAGCGTCTTGAGGAAGGT
 151 60 SYBR
BTU Tubulin
During Fermentation
Primordia
Stipes Of Fruiting Bodies
Pilei Of Fruiting Bodies
 -
F: CAGTTCACGGCGATGTTCA
R: CGACGGTAGCATCCTGGTA
 142 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Jiang Xu
Email: xj8442cn@gmail.com
Institution: The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 151, Malianwa North Road, HaiDian District, Beijing 100193, China

Reference

Identification and evaluation of reference genes for qRT-PCR normalization in Ganoderma lucidum.
Xu J, Xu Z, Zhu Y, Luo H, Qian J, Ji A, Hu Y, Sun W, Wang B, Song J, Sun C, Chen S.
Current microbiology. 2013-09-08

ICG00191-2

Tissues
Hormone Treatments
Short Time Process
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPS18 Ribosomal Protein S18
Egg
1St Larval Instar
2Nd Larval Instar
3Rd Larval Instar
4Th Larval Instar
Male
Female
Low Temperature Condition
Normal Temperature Condition
 -
F: CGCTGGTGATTCCAGATAAA
R: GACGACCTACACCTTTGATG
 111 - SYBR
EF1A Elongation Factor
Egg
1St Larval Instar
2Nd Larval Instar
3Rd Larval Instar
4Th Larval Instar
Male
Female
Low Temperature Condition
Normal Temperature Condition
 -
F: CTGGAAAGACCACAGAAGAAA
R: GAGGAGGGAATTCTTGGAAAG
 114 - SYBR
RPL4 Ribosomal Protein L4
Head
Midgut
Malpighian Tubule
Carcass
 -
F: CGTCGTCTTAACCCACTTAC
R: CTTCTTCTCTGGCCAACTG
 118 - SYBR
ACTIN Actin
Mycelia
Salicylic Acid (Sa) Treatment
Short Time Process
 KF682398.1
F: CGACCCATCCTACGAGC
R: TAGGCTGCGTCACTTGC
112 58 SYBR
UCE2 Ubiquitin Conjugating Enzyme
Mycelia
Methyl Jasmonate (Meja) Treatment
H2O2 Treatment
Short Time Process
 KF682408.1
F: ATGACCGAGTACAAGCAAC
R: CCTCCCAGGTGAAGAAG
94 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Mingwen Zhao
Email: mwzhao@njau.edu.cn
Institution: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, Jiangsu, China

Reference

Selection of reliable reference genes for RT-qPCR during methyl jasmonate, salicylic acid and hydrogen peroxide treatments in Ganoderma lucidum.
Lu X, Liu Y, Zhao L, Liu Y, Zhao M.
World journal of microbiology & biotechnology. 2018-06-12

ICG00191-3

Tissues
Hormone Treatments
Long Time Process
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
PP2A Protein Phosphatase
Mycelia
Methyl Jasmonate (Meja) Treatment
Salicylic Acid (Sa) Treatment
Long Time Process
 KF233764
F: CCTTATGGGCGACAACC
R: GCATTACCTCGTCAAACAGAT
 158 58 SYBR
UCE2 Ubiquitin Conjugating Enzyme
Mycelia
H2O2 Treatment
Long Time Process
 KF682408.1
F: ATGACCGAGTACAAGCAAC
R: CCTCCCAGGTGAAGAAG
94 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Mingwen Zhao
Email: mwzhao@njau.edu.cn
Institution: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, Jiangsu, China

Reference

Selection of reliable reference genes for RT-qPCR during methyl jasmonate, salicylic acid and hydrogen peroxide treatments in Ganoderma lucidum.
Lu X, Liu Y, Zhao L, Liu Y, Zhao M.
World journal of microbiology & biotechnology. 2018-06-12

ICG00191-4

Temperature Conditions
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACTIN Actin
High Temperature Condition
 KF682398.1
F: CGACCCATCCTACGAGC
R: TAGGCTGCGTCACTTGC
 112 58 SYBR
CYP Cytochrome
High Temperature Condition
 KF682413.1
F: CGTCGTCTTCGGCGAGGTT
R: TGCTCCGTAGCGGTGGTC
 151 58 SYBR
TIF Eukaryotic Translation Initiation Factor
High Temperature Condition
 KF682406.1
F: CATCGGTCAGACGCTGTT
R: CATGAGGATCTTCGGCAC
 175 58 SYBR
UBQ1 Ubiquitin
High Temperature Condition
 KF233768.1
F: TGCGGTGCTGGTATCTT
R: CGACAGGAGGCTTCGTC
 100 58 SYBR
UCE2 Ubiquitin Conjugating Enzyme
High Temperature Condition
 KF682408.1
F: ATGACCGAGTACAAGCAAC
R: CCTCCCAGGTGAAGAAG
 94 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Mingwen Zhao
Email: mwzhao@njau.edu.cn
Institution: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, Jiangsu, China

Reference

Identification of Reference Genes and Analysis of Heat Shock Protein Gene Expression in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, after Exposure to Heat Stress.
Liu YN, Lu XX, Ren A, Shi L, Jiang AL, Yu HS, Zhao MW.
International journal of medicinal mushrooms. 2017-01-01