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Actinobacillus suis

Basic Information

Actinobacillus suis
Accession: ICG00201
Latin Name: Actinobacillus suis
Common Name: -
Taxonomy ID: 716
Description: Actinobacillus suis is a Gram negative facultative anaerobe which is a frequent member of the normal microbiome of swine tonsils of the soft palate. It is also an important pathogen in pigs of all ages, where it can cause septicaemia and sequelae such as meningitis, arthritis, and pleuropneumonia.

Gene Summary

mRNA

ICG00201-1
pyk rpoB proS gyrA

Studies (1)

ICG00201-1

Culture Conditions
Hormone Treatments
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
pyk Pyruvate Kinase
Aerobic Treatment
Epinephrine Treatment
Brain Heart Infusion(Bhi)
 AFU19347
F: CAACTGAAGAAGCAATGGACGACA
R: AACTAACGCAGCATCACCGATTT
 - 60 SYBR
rpoB RNA Polymerase
Aerobic Treatment
Epinephrine Treatment
Brain Heart Infusion(Bhi)
 AFU20083
F: AAACGCAACAAGATCATTCAAGGTG
R: ATTTGACGACGAACCGCTAAGTAAA
 - 60 SYBR
proS Pyrroline-5-Carboxylate Reductase
Aerobic Treatment
Epinephrine Treatment
Brain Heart Infusion(Bhi)
 AFU19772
F: GTGGACAAAGCGTCATTACAAGAAAC
R: CGGAAATCTAAACCAAGACGAGTGAA
 - 60 SYBR
gyrA DNA Gyrase
Aerobic Treatment
Epinephrine Treatment
Anoxic Treatment
Anoxic Static Cultures Were Grown Without Shaking In Bhi
 AFU18440
F: ATCTGGTATTGCGGTTGGTATGG
R: TTCTTCAATGCTGATTTGCTCGTTT
 - 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Janet I MacInnes
Email: macinnes@uoguelph.ca
Institution: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, N1G 2W1 Guelph, Ontario, Canada

Reference

Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis.
Bujold AR, MacInnes JI.
BMC research notes. 2015-03-18
Created with Highcharts 10.0.0Aerobic TreatmentEpinephrine TreatmentpykrpoBproSgyrABrain Heart Infusion(Bhi)genesreferencedataAnoxic TreatmentAnoxic Static Cultures Were Grown Without Shaking In BhiCulture ConditionsHormone TreatmentsAbiotic StressesgenegrowthexpressionstabilityAerobicexperimentalconditionsvalidateculturesEpinephrineAnoxicexponentialstationaryrankingsdesignamongstablyexpressedrankedmayqPCRreal-timePCRtoolmakebestusecarefullystudyActinobacillusanalysedselectedcomparingphaseanalysingtogethertopthreesampleswellcombinedusedmultiplenormaliseBackgroundQuantitativevaluableevaluatingbacterialHoweverordermethodendogenousnormalisationmustfirstidentifiedvalidatingThereforeobjectiveeightopportunisticswinepathogensuisgrownaerobicwithoutepinephrinemediumanoxicstaticsampledphases.ResultsUsingRefFinderdeterminewhethercomprehensivevariedphasesremainedstablesetsconsistentlyrankings;rholeastset.ConclusionsReferenceassessedexperimentmindevencommonly16SrRNAdemonstratedlargevariabilitydependingstudiedpreviouslysuggestedapproachgeometricmeanresultsresearcherscontinuevariousorganismssamplingtimepointspossibleinformedpredictionssuitablegivenmeantimecautionValidationquantitativeanalysissuis.