ICG10103 Isatis tinctoria - Internal Control Genes Database (ICG)

Home Species

Isatis tinctoria

Accession: ICG10103

Latin Name: Isatis tinctoria

Common Name: woad

Taxonomy ID: 161756

Description: Isatis tinctoria, belongs to Plantae kingdom and Brassicaceae family.It is native to the grasslands and desert areas of the Caucasus, Central Asia, Eastern Siberia and West Asia. It is an important dye and medicine for humans. The biennial herb Isatis indigotica Fort. (Cruciferae) is a traditional medical plant widely cultivated in China and is popular in pharmaceuticals, food additives, flavors, and other industrial materials due to abundant bio- active components, including alkaloids , organic acids, and glycosides.

ICG10103-1

Temperature Conditions

Hormone Treatments

Injury And Repair Research Model

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
PP2A-4	Protein Phosphatase	Abscisic Acid (Aba) Treatment Wounding Stress Low Temperature Condition Root Stem Leaf Flower Fruit	c41201.graph_c0	F: GAATGCCTGCGAAAGTATGG R: TCCTAATGTTGTCAAGGGTCTC	151	83	SYBR
TUB4	Tubulin	Root Stem Leaf Flower Fruit	c43494.graph_c0	F: GCCCCGATAACTTCGTCTT R: TCAATCAACTCCGCTCCCT	85	85.8	SYBR
RPL15	Ribosomal Protein L15	Abscisic Acid (Aba) Treatment Wounding Stress Low Temperature Condition	c42633.graph_c1	F: GGGCGAAGAGGAAAGGTAG R: CGGAAGACGGCGATAAAGAG	133	84.6	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Tao Li

Email: litao@snnu.edu.cn

Institution: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shannxi Normal University, Xi’an, China

Reference

ICG10103-2

Tissues

Abiotic Stresses

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
MUB	Ubiquitin	Root Stem Leaf Petiole Low Nitrogen (Ln) Condition Abscisic Acid (Aba) Treatment Methyl Jasmonate (Meja) Treatment	At5g15460	F: TTTCCCGATGCTACAA R: ACCTGAGGCTGAATGA	215	-	SYBR
EF1-a	Elongation Factor	Root Stem Leaf Petiole Low Nitrogen (Ln) Condition Abscisic Acid (Aba) Treatment Methyl Jasmonate (Meja) Treatment	At1g07930	F: GCCGATTGTGCTGTCC R: GTGGCATCCATCTTGTTA	149	-	SYBR
TIP41	Tonoplast Intrinsic Protein	Low Nitrogen (Ln) Condition Methyl Jasmonate (Meja) Treatment	At4g34270	F: AACACTTGAGCAGCAA R: GTATGGCGTCGTAAAA	173	-	SYBR
ACT	Actin	Abscisic Acid (Aba) Treatment	At3g53750	F: GCTCACGGAAGCACCT R: CGACCACTAGCGTAAAGT	128	-	SYBR
CYP	Cytochrome	Root Stem Leaf Petiole	At2g29960	F: GGCAAGACAGTTCCTA R: TGATTCTCCACCCATA	177	-	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Xiaoqing Tang

Email: xqtang@njau.edu.cn

Institution: College of Horticulture, Nanjing Agricultural University, Nanjing 210095

Basic Information

Gene Summary

mRNA

Studies (2)

ICG10103-1

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG10103-2

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG10103-1	PP2A-4 TUB4 RPL15
ICG10103-2	MUB EF1-a TIP41 ACT CYP