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Avena sativa

Basic Information

Avena sativa
Accession: ICG10215
Latin Name: Avena sativa
Common Name: hexaploid oat
Taxonomy ID: 4498
Description: Oat (Avena sativa L.) is an allohexaploid (2n = 6 × = 42) cereal crop with estimated 13 Gb genome. With an upswing in food and industrial utilization, oats are now cultivated worldwide and form an important dietary staple on a global scale.

Gene Summary

mRNA

ICG10215-2
EF1-a TBP2 PSKS1 UBC2
ICG10215-1
EIF4A UBC21 EP

Studies (2)

ICG10215-1

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
EIF4A Eukaryotic Initiation Factor
Endosperm
Seed
 -
F: GTGAAGATGATTCCCACCAAGC
R: CCTCATGTCACGCACAGCAAA
 87 60.9/62.7 SYBR
UBC21 Ubiquitin
Shoot
Root
Seed
 -
F: GCCCATCGGAGACACCTTTTG
R: CCTGTCTTGAAGTGAACATTTGG
 133 64/59.1 SYBR
EP Uncharacterized Protein
Seed
 -
F: GCACAAGTGATGCCAGAATAGC
R: CGAGATGCATTAGATTCGTTGG
 193 60/60.1 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Meng Zhang
Email: zhangm@nwsuaf.edu.cn
Institution: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China

Reference

Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.).
Yang Z, Wang K, Aziz U, Zhao C, Zhang M.
Plant methods. 2020-10-15

ICG10215-2

Abiotic Stresses
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
EF1-a Elongation Factor
Root
Uv Irradiation Stress
 -
F: AGCCTGGTATGGTTGTGACC
R: GCTTGAGATCCTTCACGGCA
 147 58 SYBR
TBP2 TATA-binding Protein
Stem
Uv Irradiation Stress
 -
F: GTGGATCTGTTGAGGCACCC
R: ATACTCTGCATTGCGCGCTT
 123 58 SYBR
PSKS1 Protein PSK SIMULATOR
Leaf
Root
Uv Irradiation Stress
High-light Stress
 -
F: TGGAGAGTGGGCAAATACAGG
R: AAGCCACACAACAAGCTCAAG
 136 58 SYBR
UBC2 Ubiquitin
Stem
High-light Stress
 -
F: ACAGCGTTCCGAGAGTTGTT
R: AAGAGTCAGCCCGAATGCAG
 104 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Guowen Cui
Email: cgw603@neau.edu.cn
Institution: Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Reference

Validation of Appropriate Reference Genes for qRT-PCR Normalization in Oat (Avena sativa L.) under UV-B and High-Light Stresses.
Yin H, Yin D, Zhang M, Gao Z, Tuluhong M, Li X, Li J, Li B, Cui G.
International journal of molecular sciences. 2022-09-23