View source for NONHSAT129019

''PRNCR1''(PCNCR1), is pervasively transcribed from the 8q24 “gene desert” region in strong association with susceptibility of prostate cancer.

==Annotated Information==
===Name===
''PRNCR1'': prostate cancer associated non-coding RNA 1(HGNC nomenclature)

===Characteristics===
PRNCR1(prostate cancer non-coding RNA 1) , ∼13 kb intron-less long non-coding RNA (ncRNA) ,was transcribed in centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) on 8q24 hat ,a large “gene-desert” ,whose region is susceptibility to prostate cancer<ref name="ref1" />.

PRNCR1 (also known as PCAT8) and PCGEM1, bind successively to the androgen receptor and strongly enhance both ligand-dependent and ligand-independent androgen-receptor-mediated gene activation programs and proliferation in prostate cancer cells<ref name="ref2" />.

===Function===
PRNCR1 overexpression can potentially serve as a required component of castration-resistance in prostatic tumors<ref name="ref2" />.

the polymorphisms in the lncRNA PRNCR1 may be related to the development of CRC, offering a novel and potential strategy for functional analysis of susceptibility loci to human diseases<ref name="ref3" />.

===Diseases===
* prostate cancer<ref name="ref1" /><ref name="ref2" />
* colorcetal cancer<ref name="ref3" />

===Expression===
PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia<ref name="ref1" />.

The primers for real-time quantitative PCR were as follows,
{|class='wikitable' style="text-align:center"
|-
! | Primer
! | Forward
! | Reverse
|-
| | PRNCR1 
| | 5'-CCAGGG-GGAAACACACAG-3'
| | 5'-AAATGGCAGTTTCCTTCAATG-3'<ref name="ref1" />
|-
| | β-actin (ACTB)
| | 5'-TTGGCTTGACTCAGGATTTA-3'
| | 5'-ATGCTATC-ACCTCCCCTGTG-3'<ref name="ref1" />
|}
 
siRNA target sequences of were as follows,:
{|class='wikitable' style="text-align:center"
|-
! | Primer
! | Sequences
|-
| | si1
| |5'-GCUCUUAAGGAAAUAACUU-3'<ref name="ref1" />
|-
| | si2
| | 5'-GACGGUUCUGUUUAAGUAA-3'<ref name="ref1" />
|-
| | siEGFP(as a negative control)
| | 5'-GAAGCAGCACGACUUCUUC-3'<ref name="ref1" />
|}
The knockdown effect was measured by RT-PCR using the primer set: 5′-ATTGTTCCCAGTTCTCTGGAC-3′ and 5′-GGAGGAAGTGGAGTAGGTATAAGAG-3′. 
===Allelic Information and Variation===
The transcript is an approximately 13-kb intron-lessnon-coding RNA spanning a region between rs1016342 andrs7463708, and named this novel ncRNA as PRNCR1(prostate cancer non-coding RNA 1, GenBank Accession no.AB458446).<ref name="ref1" />

==Labs working on this lncRNA==
* Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan<ref name="ref1" />.

* Howard Hughes Medical Institute, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA<ref name="ref2" />.

* Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children's Health; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P,R, China<ref name="ref3" />.

==References==
<references>
<ref name="ref1">Chung S, Nakagawa H, Uemura M, Piao L, Ashikawa K, Hosono N, et al. Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility[J]. Cancer science. 2011,102(1):245-52.</ref>(1)
<ref name="ref2">Yang L, Lin C, Jin C, Yang JC, Tanasa B, Li W, et al. lncRNA-dependent mechanisms of androgen-receptor-regulated gene activation programs[J]. Nature. 2013,500(7464):598-602.</ref>(2)
<ref name="ref3">Li L, Sun R, Liang Y, Pan X, Li Z, Bai P, et al. Association between polymorphisms in long non-coding RNA PRNCR1 in 8q24 and risk of colorectal cancer[J]. Journal of experimental & clinical cancer research : CR. 2013,32:104.</ref>(3)
</references>

{{basic|
tID = NONHSAT129019|
source = NONCODE4.0|
same = ''PRNCR1'',|
classification = intergenic|
length = 1248 nt|
location = chr8-:128098508..128099755|
number = 1|
exons = 128098508..128099755|
context = <html><div align="center">
<iframe src="http://lncrna.big.ac.cn/view/?data=species/human&loc=chr8:128098508..128099755&tracklist=0&overview=0&tracks=DNA,RefGene,lncRNA" style=" border-width:0 " width="100%" height="250" scrolling="yes"></iframe>
</div></html>|
sequence = <dnaseq>CAGCAGAGACGCCAATACAGACGACAAAACCAGCAGTCTTCATCTGACTCTGGTTCCTCCTTCTCCTCAGAAGATGAACGACCCAAGAGATCCCATGTGAAGAATGGTGAGGTTGGCAAGCGGCGGAGACATTCCCCTTCCCGGAGTGCTTCTCCATCACCACGAAAGCACCAAAAAGAGACTTCCCCTCATGGTAGACGGAGGAGAAGTCCATCCCCACCACCCACCAGAAGGCGACGGTCTCCTTCTCCCGCCCCTCATCCTCGACGGCTCAGGACTCCCACACCACCACCACCACGACGAAGGACTCCTTCTCCTCCCCTACGTCGGCGCTCACCTTCTCCTAGAAGATACTCTCCTCCAATACAGAGGAGATACTCTCCTTCTCCACCTCCAAAGAGAAGAACGGCTTCACCTCCTCCCCCTCCTAAACGAAGAGCATCACCATTTCCACCACCAAAGCGGCGGGTCTCCCATTCTCCACCTTCCAAAGAAGCTCCCCAGTCACCAAGAGACGTTCACCTTCACTATCATCCAAGCATAGGAAAGGGTCTTCCCCAAGCCGCTCTACCCGGGAGGCCCGATCACCGCATCCAAACAAATGGCATTCGCCCTCATCACGGCCTCGAGCTCCTCAGACCTCAAGTCCTCCACCCGTTCGAAGAGGAGCGTCGTCATCACCCCAAAGAAGGCAGTCCCCGTCTCCAAGTACTAGGCCCATTAGGAGAGTCTCCAGGACTCCGGAACCTAAAAAGATAAAAAAAGGCTGCTTCCCCAAGCCCACAGTCTGTAAGGAGGGTCTCATCCTCCCGATCTGTCTCTGGGTCTCCTGAGCCAGCAGCTAAAAAGCCCCCAGCATCTCCATCCCCCATCCAGTCTCAGTCACCGTCTACAAACTGGTCACCAGCTGTACCGGTCAAAAAGGCCAAAAGCCCAACACCGAGCCCATCACTGCCAAGAAATTCAGATCaagaaggagatggaaagaaaaagaagaaaaagaaggacaggaaacacaaaaaggataagaagcacaagaagcacaaaaaacacaagaaggaaaaggctgtggctgcagctgctgcagctgctgtgacccctgcagccattgcagctgcCACAACCACATTAGCACAGGAAGAGCCAGTGGCAGCGCCAGAGCCGAAGAAGGAGACTGAAAGTGAAGCTGAAGATAACCTTGATGATTTAGAAAAGCACCTGCGTGAAACGGCCCCGAGATCAATGAGG</dnaseq>|
}}
[[Category:Intergenic]][[Category:NONHSAG051247]][[Category:Transcripts]]