Difference between revisions of "NONHSAT099821"

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Please input one-sentence summary here.
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''DBE-T'', a chromatin-associated noncoding RNA expressed selectively in FSHD patients, directly recruits ASH1L to the FSHD locus to co-ordinate 4q35 gene de-repression<ref name="ref1" />
  
 
==Annotated Information==
 
==Annotated Information==
 
===Transcriptomic Nomeclature===
 
===Transcriptomic Nomeclature===
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Please input transcriptomic nomeclature information here.
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''DBE-T'':D4Z4 binding element transcript(HGNC nomenclature)
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"chromatin-associated long non-coding RNA", DBE-T, DUX4L30<ref name="ref1" />
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===Cellular Localization===
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nuclear<ref name="ref1" />.
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===Function===
 
===Function===
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[[File: DBE-T.jpg|right|thumb|400px|'''Proposed model of action of ''DBE-T'' lncRNA''' <ref name="ref1" />.]]
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''DBE-T'' recruits the Trithorax group protein Ash1L to the FSHD locus, driving histone H3 lysine 36 dimethylation, chromatin remodeling, and 4q35 gene transcription<ref name="ref1" />.
  
 
===Regulation===
 
===Regulation===
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Please input regulation information here.
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Ash1L promotes the expression of DBE-T, implying a positive feedback loop that would sustain 4q35 gene de-repression<ref name="ref1" />.
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===Diseases===
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* Facioscapulohumeral muscular dystrophy (FSHD)<ref name="ref1" />
  
 
===Expression===
 
===Expression===
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Please input expression information here.
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''DBE-T'' knockdown prevented 4q35 gene de-repression caused by AZA plus TSA, strongly suggesting a functional role for this transcript in the positive regulation of 4q35 gene expression<ref name="ref1" />
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===Allelic Information and Variation===
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{| class='wikitable' style="text-align:center"
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Please input allelic information and variation information here.
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|-
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! | shRNA
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! | Primer
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|-
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| rowspan="1"|DBE
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| | GCTCACCGCCATTCATGAAGG<ref name="ref1" />
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|-
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| rowspan="1"|NDE
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| | GCTCACCGCCATTCATGAAGG<ref name="ref1" />
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|-
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| rowspan="1"|Nonsilencing
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| | TCTCGCTTGGGCGAGAGTAAG<ref name="ref1" />
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|-
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| rowspan="1"|Ash1L
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| | GCTGGTCATTTATTGCTCAAT<ref name="ref1" />
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|-
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| rowspan="1"|Suz12
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| | TTCTACAAACAGCATACAG<ref name="ref1" />
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|}
  
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===Evolution===
 
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You can also add sub-section(s) at will.
 
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
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Please input related labs here.
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Dulbecco Telethon Institute at San Raffaele Scientific Institute, Division of Regenerative Medicine, Stem Cells, and Gene Therapy, 20132 Milan, Italy
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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<ref name="ref1">Cabianca DS, Casa V, Bodega B, Xynos A, Ginelli E, Tanaka Y, et al. A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy[J]. Cell. 2012,149(4):819-31.</ref>(1)
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</references>
  
 
{{basic|
 
{{basic|
 
tID = NONHSAT099821|
 
tID = NONHSAT099821|
 
source = NONCODE4.0|
 
source = NONCODE4.0|
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same = ,|
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same =''DBE-T'' ,"chromatin-associated long non-coding RNA", DBE-T, DUX4L30|
 
classification = intergenic|
 
classification = intergenic|
 
length = 682 nt|
 
length = 682 nt|

Revision as of 15:21, 3 April 2015

DBE-T, a chromatin-associated noncoding RNA expressed selectively in FSHD patients, directly recruits ASH1L to the FSHD locus to co-ordinate 4q35 gene de-repression[1]

Annotated Information

Transcriptomic Nomeclature

DBE-T:D4Z4 binding element transcript(HGNC nomenclature)

"chromatin-associated long non-coding RNA", DBE-T, DUX4L30[1]

Cellular Localization

nuclear[1].


Function

Proposed model of action of DBE-T lncRNA [1].

DBE-T recruits the Trithorax group protein Ash1L to the FSHD locus, driving histone H3 lysine 36 dimethylation, chromatin remodeling, and 4q35 gene transcription[1].

Regulation

Ash1L promotes the expression of DBE-T, implying a positive feedback loop that would sustain 4q35 gene de-repression[1].

Diseases

  • Facioscapulohumeral muscular dystrophy (FSHD)[1]

Expression

DBE-T knockdown prevented 4q35 gene de-repression caused by AZA plus TSA, strongly suggesting a functional role for this transcript in the positive regulation of 4q35 gene expression[1]


shRNA Primer
DBE GCTCACCGCCATTCATGAAGG[1]
NDE GCTCACCGCCATTCATGAAGG[1]
Nonsilencing TCTCGCTTGGGCGAGAGTAAG[1]
Ash1L GCTGGTCATTTATTGCTCAAT[1]
Suz12 TTCTACAAACAGCATACAG[1]


Labs working on this lncRNA

Dulbecco Telethon Institute at San Raffaele Scientific Institute, Division of Regenerative Medicine, Stem Cells, and Gene Therapy, 20132 Milan, Italy

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Cabianca DS, Casa V, Bodega B, Xynos A, Ginelli E, Tanaka Y, et al. A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy[J]. Cell. 2012,149(4):819-31.

Basic Information

Transcript ID

NONHSAT099821

Source

NONCODE4.0

Same with

DBE-T ,"chromatin-associated long non-coding RNA", DBE-T, DUX4L30

Classification

intergenic

Length

682 nt

Genomic location

chr4+:190986388..190987069

Exon number

1

Exons

190986388..190987069

Genome context

Sequence
000001 GCCTGGGGCT CTCCCACAGG GGGCTTTCGT GAGCCAGGCA GCGAGGGCCG CCCCCGCGCT GCAGCCCAGC CAGGCCGCGC 000080
000081 CGGCAGAGGG GATCTCCCAA CCTGCCCCGG CGCGCGGGGA TTTCGCCTAC GCCGCCCCGG CTCCTCCGGA GCCGGGGCGC 000160
000161 TCTCCCACCC TCAGGCTCCT CGGTGGCCTC CGCACCCGGG CAAAAGCCGG GAGGACCGGG ACCCGCAGCG CGACGGCCTG 000240
000241 CCGGGCCCCT GCGCGGTGGC ACAGCCTGGG CCCGCTCAAG CGGGGCCGCA GGGCCAAGGG GTGCTTGCGC CACCCACGTC 000320
000321 CCAGGGGAGT CCGTGGTGGG GCTGGGGCCG GGGTCCCCAG GTCGCCGGGG CGGCGTGGGA ACCCCAAGCC GGGGCAGCTC 000400
000401 CACCTCCCCA GCCCGCGCCC CCCGGACGCC TCCGCCTCCG CGCGGCAGGG GCAGATGCAA GGCATCCCGG CGCCCTCCCA 000480
000481 GGCGCTCCAG GAGCCGGCGC CCTGGTCTGC ACTCCCCTGC GGCCTGCTGC TGGATGAGCT CCTGGCGAGC CCGGAGTTTC 000560
000561 TGCAGCAGGC GCAACCTCTC CTAGAAACGG AGGCCCCGGG GGAGCTGGAG GCCTCGGAAG AGGCGCCTCG CTGGAAGCAC 000640
000641 CCCTCAGCGA GGAAGAATAC CGGGCTCTGC TGGAGGAGCT TT
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