Difference between revisions of "NONHSAT087920"

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Please input one-sentence summary here.
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GHRLOS, which gives rise to endogenous ghrelin natural antisense transcripts. exhibits features which are common to many non-coding RNA genes, including extensive splicing, lack of significant and conserved open reading frames, differential expression and lack of conservation in vertebrates.
  
 
==Annotated Information==
 
==Annotated Information==
 
===Transcriptomic Nomeclature===
 
===Transcriptomic Nomeclature===
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Please input transcriptomic nomeclature information here.
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GHRLOS:ghrelin opposite strand/antisense RNA(HGNC nomenclature)
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"GHRL antisense RNA 1 (non-protein coding)", GHRL-AS1, NCRNA00068, "non-protein coding RNA 68"<ref name="ref1" />
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===Characteristics===
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[[File:Mapping of GHRLOS transcript 5' and 3' ends.jpg|right|thumb|400px|'''Mapping of GHRLOS transcript 5' and 3' ends.'''<ref name="ref1" />]]
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GHRLOS transcripts that extend over 1.4 kb of the promoter region and 106 nucleotides of exon 4 of the ghrelin gene(GHRL) initiate 4.8 kb downstream of the terminal exon 4 of GHRL and are present in the 3' untranslated exon of the adjacent gene TATDN2 (TatD DNase domain containing 2). The 3' region of GHRLOS is also antisense, in a tail-to-tail fashion to a novel terminal exon of the neighbouring SEC13 gene, which is important inprotein transport. GHRLOS that is riddled with stop codons is little nucleotide and amino-acid sequence conservation between vertebrates. The gene spans 44 kb on 3p25.3, is extensively spliced and harbours multiple variable exons<ref name="ref1" />.
  
 
===Function===
 
===Function===
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Please input function information here.
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GHRLOS RNA transcripts display several distinctive features of non-coding (ncRNA) genes, including 5' capping, polyadenylation, extensive splicing and short open reading frames<ref name="ref1" />.  
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GHRLOS natural antisense transcripts, functioning as a non-coding RNA,may regulate one or both of these genes, because GHRLOS overlaps the terminal exons of both GHRL andSEC13-T<ref name="ref1" />
  
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===Regulation===
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===Expression===
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Please input regulation information here.
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We have also investigated the expression of GHRLOS and found evidence of differential tissue expression. It is highly expressed in tissues which are emerging as major sites of non-coding RNA expression (the thymus, brain, and testis), as well as in the ovary and uterus. In contrast, very low levels were found in the stomach where sense, GHRL derived RNAs are highly expressed.
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For non-quantitative RT-PCR analysis of GHRLOS splicing, RT-PCRs were performed with a forward primer in a region common to the 5' terminal exon Ia/b and a reverse primer in the 3' terminal exon 4 of GHRLOS<ref name="ref1" />.
  
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===Expression===
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{|class='wikitable' style="text-align:center"
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Please input expression information here.
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|-
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| rowspan="1"| Ito4-F
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| | CATGGAAGTCTCCAAGCCTG<ref name="ref1" />
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|-
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| rowspan="1"| Ito4-R
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| | CTGCTCTACTGCCTCAATGTC<ref name="ref1" />
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|}
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To detect long, chimaeric transcripts, we employed RT-PCR with a forward primer in exon 2 of TATDN2(ChiOut-F) and a reverse primer in exon 1 of GHRLOS<ref name="ref1" />.
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{|class='wikitable' style="text-align:center"
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|-
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| rowspan="1"| ChiOut-F
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| | TGAAAGCCCAGAAGGAGGA<ref name="ref1" />
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|-
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| rowspan="1"| ChiOut-R
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| | TCTAAGTTTAGAAGCGCTCATCTG<ref name="ref1" />
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|}
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To allow strand-specific and RNA-specific amplification of GHRLOS transcripts, reverse transcription was performed using a gene-specific primer in exon 4 with a linker (LK) sequence attached to the 5' end of the primer<ref name="ref1" />.
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{|class='wikitable' style="text-align:center"
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|-
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| rowspan="1"| GHRLOS-Real-F
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| | CATTGAGGCAGTAGAGCAGTTGA<ref name="ref1" />
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|-
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| rowspan="1"| LK
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| | CGACTGGAGCACGAGGACACTGA<ref name="ref1" />
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|}
  
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===Allelic Information and Variation===
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To detect sense GHRL transcripts,a strand-specific RT-PCR approach was employed, with a reverse transcription primer spanning the 3' terminal exon 4 of the ghrelin gene (GHRLex4_RT_LK) followed by PCR with an exon 4 specific forward primer (GHRLex4_F) and a linker-specific reverse primer(LK)<ref name="ref1" />.  
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Please input allelic information and variation information here.
 
  
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===Evolution===
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{|class='wikitable' style="text-align:center"
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|-
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| rowspan="1"| GHRL-Real-RT-LK
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| | CGACTGGAGCACGAGGACACTGAGCCAGAGAGCGCTTCTAAACTTA<ref name="ref1" />
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|-
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| rowspan="1"| GHRL-Real-F
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| | GCCCCAGCCGACAAGTG<ref name="ref1" />
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|-
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| rowspan="1"| LK
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| | CGACTGGAGCACGAGGACACTGA<ref name="ref1" />
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|}
  
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==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
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Please input related labs here.
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Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia<ref name="ref1" />.  
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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<ref name="ref1">Seim I, Carter SL, Herington AC, Chopin LK. Complex organisation and structure of the ghrelin antisense strand gene GHRLOS, a candidate non-coding RNA gene[J]. BMC molecular biology. 2008,9:95.</ref>(1)
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</references>
  
 
{{basic|
 
{{basic|
 
tID = NONHSAT087920|
 
tID = NONHSAT087920|
 
source = NONCODE4.0|
 
source = NONCODE4.0|
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same = ,|
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same = GHRLOS, GHRL-AS1, NCRNA00068|
 
classification = intergenic|
 
classification = intergenic|
 
length = 1765 nt|
 
length = 1765 nt|

Revision as of 16:55, 9 April 2015

GHRLOS, which gives rise to endogenous ghrelin natural antisense transcripts. exhibits features which are common to many non-coding RNA genes, including extensive splicing, lack of significant and conserved open reading frames, differential expression and lack of conservation in vertebrates.

Annotated Information

Transcriptomic Nomeclature

GHRLOS:ghrelin opposite strand/antisense RNA(HGNC nomenclature)

"GHRL antisense RNA 1 (non-protein coding)", GHRL-AS1, NCRNA00068, "non-protein coding RNA 68"[1]

Characteristics

Mapping of GHRLOS transcript 5' and 3' ends.[1]

GHRLOS transcripts that extend over 1.4 kb of the promoter region and 106 nucleotides of exon 4 of the ghrelin gene(GHRL) initiate 4.8 kb downstream of the terminal exon 4 of GHRL and are present in the 3' untranslated exon of the adjacent gene TATDN2 (TatD DNase domain containing 2). The 3' region of GHRLOS is also antisense, in a tail-to-tail fashion to a novel terminal exon of the neighbouring SEC13 gene, which is important inprotein transport. GHRLOS that is riddled with stop codons is little nucleotide and amino-acid sequence conservation between vertebrates. The gene spans 44 kb on 3p25.3, is extensively spliced and harbours multiple variable exons[1].

Function

GHRLOS RNA transcripts display several distinctive features of non-coding (ncRNA) genes, including 5' capping, polyadenylation, extensive splicing and short open reading frames[1]. GHRLOS natural antisense transcripts, functioning as a non-coding RNA,may regulate one or both of these genes, because GHRLOS overlaps the terminal exons of both GHRL andSEC13-T[1]

Expression

We have also investigated the expression of GHRLOS and found evidence of differential tissue expression. It is highly expressed in tissues which are emerging as major sites of non-coding RNA expression (the thymus, brain, and testis), as well as in the ovary and uterus. In contrast, very low levels were found in the stomach where sense, GHRL derived RNAs are highly expressed. For non-quantitative RT-PCR analysis of GHRLOS splicing, RT-PCRs were performed with a forward primer in a region common to the 5' terminal exon Ia/b and a reverse primer in the 3' terminal exon 4 of GHRLOS[1].

Ito4-F CATGGAAGTCTCCAAGCCTG[1]
Ito4-R CTGCTCTACTGCCTCAATGTC[1]

To detect long, chimaeric transcripts, we employed RT-PCR with a forward primer in exon 2 of TATDN2(ChiOut-F) and a reverse primer in exon 1 of GHRLOS[1].

ChiOut-F TGAAAGCCCAGAAGGAGGA[1]
ChiOut-R TCTAAGTTTAGAAGCGCTCATCTG[1]

To allow strand-specific and RNA-specific amplification of GHRLOS transcripts, reverse transcription was performed using a gene-specific primer in exon 4 with a linker (LK) sequence attached to the 5' end of the primer[1].

GHRLOS-Real-F CATTGAGGCAGTAGAGCAGTTGA[1]
LK CGACTGGAGCACGAGGACACTGA[1]

To detect sense GHRL transcripts,a strand-specific RT-PCR approach was employed, with a reverse transcription primer spanning the 3' terminal exon 4 of the ghrelin gene (GHRLex4_RT_LK) followed by PCR with an exon 4 specific forward primer (GHRLex4_F) and a linker-specific reverse primer(LK)[1].

GHRL-Real-RT-LK CGACTGGAGCACGAGGACACTGAGCCAGAGAGCGCTTCTAAACTTA[1]
GHRL-Real-F GCCCCAGCCGACAAGTG[1]
LK CGACTGGAGCACGAGGACACTGA[1]


Labs working on this lncRNA

Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia[1].

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Seim I, Carter SL, Herington AC, Chopin LK. Complex organisation and structure of the ghrelin antisense strand gene GHRLOS, a candidate non-coding RNA gene[J]. BMC molecular biology. 2008,9:95.

Basic Information

Transcript ID

NONHSAT087920

Source

NONCODE4.0

Same with

GHRLOS, GHRL-AS1, NCRNA00068

Classification

intergenic

Length

1765 nt

Genomic location

chr3+:10322636..10335133

Exon number

5

Exons

10322636..10322686,10327432..10327537,10329098..10329165,10329629..10329786,10333752..10335133

Genome context

Sequence
000001 ACATGGAAGT CTCCAAGCCT GTGCCATCCA CCTGCCAAGG AAAAGCACAA GCATACAGTT TGAACATTTA TTCGCCTCCT 000080
000081 GAGCTTGTAC AACAGTCGTG GGAGTTGCTG CAGAAGCAAG CGAAAAGCCA GATGAGCGCT TCTAAACTTA GAGAGAGGGA 000160
000161 GAGCGCCTCA TCTCTTCCAT TTTCCAGGTA TTCCTGAGAT GATTTATTGG AGCTCAAAGC TTTGGGAGAG TTGGGGCCTT 000240
000241 CCATTCCCTC CAGTAAATAC TTGTTTTTCT TCCACCGCTG AGGCAAATGC GGGGTGGCTG ATCACCTGGC AGACATCTTA 000320
000321 GGAAACAGGA GCACCGGTCT GGGAAACTGC TGGCCTGGCC TAACACCTGG CGCTGTGGTG CAGGAGGGCA GCCACTGAGG 000400
000401 ATTCTTGAGG AGAGAGAGac catagaatga atgaaagagt tggaaagact ttaaagctct gggaggctga atcctttatt 000480
000481 tcgctggaag aaaaactgaa tgccaagagg gcctcacttt ccccaaagct atacagccat caggggcaat gctgggattc 000560
000561 ccccatggat ctcttgactc ctaatacagt gctctttctg atatgccatc tgGCTCCATA ATGAACATTG TGTTCCAGGA 000640
000641 AAATCAGTCT GCGCTGAACA GGATGGAATT GGGGCAGGAC GTCCAGTGAG GAGGACATTG AGGCAGTAGA GCAGTTGATT 000720
000721 GCCGAATGAC CACCTACCCT GACTTAAAAG ATCAACCTCA GGGAGGATTG GAGCTTTCTA GAGTCTCTTG GGACAGCAGA 000800
000801 AGCACAGGCC GGGTTGGACT GAATCTTTAA GTGGACATGA GGGACAAAGT ACCTCCTGTT GGTAAACATC TCACCACCAA 000880
000881 CCCACTGTGG TGGCTAAAAT CCCACCTTTA GTCCCAGCAA CATATGAGCA TGTCACCAGG AGGTCTTCAC AGGCCTGTCT 000960
000961 GCCACCTGAG TGTAGACATC TTTTGGCCCT GGAGCCCAGA GAGGCTGAAT GTGGAGAGGG TGGAGAGAGG TCTGTAGTCC 001040
001041 TCAGAGAAGA CTTGCAGCTT TTTCAGAGCC ACCAACCCAT AAAAAAAAAA ATCCCCAAAC AGAAAAATCC TAACTGTGGT 001120
001121 GACCAGGTAC CTCCTGAGAC ATGAAGCCTC CACTTACCTG GACCCTGGAG GCCTCTCCGG GCACAGCTGC CAATGTTGAT 001200
001201 CTTAGATAAG ACCACCAGCA AGTAAACATC CACTGTGCAA AGCTGTGTTA TCTTTGGGGA ACTGAAATGT CCCCTGGGAG 001280
001281 TTGGAAACTC CCCTAGCCAC ATACCACAGA GTTGAGGAAG GAAGAGCTGA TGGACGG