Difference between revisions of "ENST00000433810.1"
Chunlei Yu (talk | contribs) (→Function) |
Chunlei Yu (talk | contribs) (→Function) |
||
| Line 11: | Line 11: | ||
===Function=== | ===Function=== | ||
| − | + | [[File:Binding of lncUSMycN to the RNA-binding protein NonO .jpg|right|thumb|400px|'''Binding of lncUSMycN to the RNA-binding protein NonO'''<ref name="ref1" />]] | |
LncUSMycN RNA bounding to the RNA-binding protein NonO, leading to N-Myc RNA upregulation, which in turns leads to post-transcriptional upregulation of N-Myc proteian expression and induces the proliferation of neuroblastoma cell, may contributes to tumorigenesis<ref name="ref1" />. | LncUSMycN RNA bounding to the RNA-binding protein NonO, leading to N-Myc RNA upregulation, which in turns leads to post-transcriptional upregulation of N-Myc proteian expression and induces the proliferation of neuroblastoma cell, may contributes to tumorigenesis<ref name="ref1" />. | ||
Revision as of 07:05, 14 April 2015
lncUSMycN,a 1040 bp long non-coding RNA,locating in proximity (14kb upstream) to the MYCN transcription start site,contributes to tumorigenesis.
Contents
Annotated Information
Transcriptomic Nomeclature
MYCNUT: MYCN upstream transcript (non-protein coding)(HGNC nomenclature)
lncUSMycN[1]
Charicteristics
The lncUSMycN gene, which is located in proximity (14kb upstream) to the MYCN transcription start site.[1].
Function
LncUSMycN RNA bounding to the RNA-binding protein NonO, leading to N-Myc RNA upregulation, which in turns leads to post-transcriptional upregulation of N-Myc proteian expression and induces the proliferation of neuroblastoma cell, may contributes to tumorigenesis[1].
Diseases
- Neuroblastoma, tumorigenesis[1]
Expression
Long noncoding RNA USMycN (lncUSMycN) is frequently coamplified with the 130-kb amplicon containing MYCN and consecutively overexpressed in MYCN oncogene–amplified neuroblastoma[1].
LNA-ASO targeting lncUSMycN (5’-TGAGGGTTGAGTTGGT-3’) and negative control LNA-ASO (5’-GACGGTAACTAGGCGA-3’) were designed and synthesized by Exiqon (Exiqon, Vedbaek, Denmark).
Specific primers in RT-PCR used as follows,
| Primer | Forward | Reverse |
|---|---|---|
| lncUSMycN | 5'-ACAAATGAGGCAACAAGCAACTT-3' | 5'-CTTTGGTGCTCAAGCCTTCAG-3'[1] |
| β-actin | 5'-CACCATGTACCCTGGCATT-3' | 5'-ACGGAGTACTTGCGCTCAG-3'[1] |
Labs working on this lncRNA
- Children's Cancer Institute Australia for Medical Research, Randwick NSW, Australia[1].
References
Basic Information
| Transcript ID |
ENST00000433810.1 |
| Source |
Gencode19 |
| Same with |
lnc-MYCN-1:1,NONHSAT069300,MYCNUT, lncUSMycN |
| Classification |
intergenic |
| Length |
1039 nt |
| Genomic location |
chr2+:16060521..16076139 |
| Exon number |
3 |
| Exons |
16060521..16061185,16061690..16061819,16075896..16076139 |
| Genome context |
|
| Sequence |
000001 ACTAAACCAG GACACTTTTG AGAATGAACT TGGGTGCTAT TGGTAATCAC ACCGAGACAC AGCAACGTCC GGGACCAACT 000080
000081 CAACCCTCAG TCCGCTCATG GGGCACAGGT TTCCTGGTCC TTCCCTACCC TGACTTCTCT TCTTCTGATG TTTGGTGGTC 000160 000161 TGCATCCTTT TAAACCACTG TGCTCCCAGA ACTGATTCCC ATATGTGTCT GCCATACTGG AAAATACCAA ATAGGCAAGG 000240 000241 AGATCGTTTC AATACTGTCT GCTGAACACC TTAATAGAGG CAAGAGAGAA CGCAGAGGAA GAAACAATGG ACTCTTTCTG 000320 000321 AGAGAGTGGA AGATGGCTTC GCAGAGGAGC TGCTGTCTGA GACGGATAGC ATGAGTAGGA GTTTCCCCAG GCAGGGAAGG 000400 000401 TGTTGTTGTT TCTCCTCAGG GTAGAGGGGA GAGCAGGGTT CCCCGTGTGT CTGTTGCTGA ATGTGACTCA TGCTGTTTTC 000480 000481 CTGCTTTGAG CCTGCTCTAG GGTGTTATGG GCAGGATGGA CTTGTCCTGC GTGCTTGTTG TTCCGCCGTG TTTCTGATCT 000560 000561 TCAGATTTAT CGTAAGGAAG AACAGCCGAT GTACAGGCAG AACTTGACAA ATGAGGCAAC AAGCAACTTG ATCTGGGATC 000640 000641 CATCGTCCTC TAGACCCCAG TAGGAGTCTA AGCACCAGCG TGGTGGCCAC CCCAGCAGCC TTGGACCCCT GAAGGCTTGA 000720 000721 GCACCAAAGC ACACACAGCT GCCTCTGGCT GAGTTCTGTG GTCGCCTGCA CCCCCTCCAC CTGCTTCCCC CGGAGGATGG 000800 000801 ATCATGAAGA GTGGAATTGC TTTTCCTCTT GGGGCAGGCA CACCAACAGT GTATGTGAGG CCCCTGCTCC CACTCCCCAG 000880 000881 GCTGGCCAGT ACTTTGGGCC AATCTGCTAG ACCCAAAAAT GTTAATCTCA CTGAGATTTA ATTTGCATTC CCCTGACTAC 000960 000961 CGGTGAAGTT GAGCATCTTT TCATATGTTT ATTGACAATT TGCATTTCTT ATTCAGTAAA TTACCTGCTC GTATCCTAG |
