Difference between revisions of "NONHSAT112472"
Chunlei Yu (talk | contribs) |
Chunlei Yu (talk | contribs) (→Expression) |
||
| Line 14: | Line 14: | ||
TDRG1 is specially expressed in spermatogenic cells, with highest expression level during human puberty stage, and decresing levels of expression with aging<ref name="ref1" />. | TDRG1 is specially expressed in spermatogenic cells, with highest expression level during human puberty stage, and decresing levels of expression with aging<ref name="ref1" />. | ||
Primers for regular RT-PCR and real time RT-PCR were as follows, | Primers for regular RT-PCR and real time RT-PCR were as follows, | ||
| − | {class='wikitalbe' style="text-align:center" | + | {|class='wikitalbe' style="text-align:center" |
|- | |- | ||
! | Primers | ! | Primers | ||
| Line 28: | Line 28: | ||
| | 5'-TCTAGACGGCAGGTCAGGTCACC-3'<ref name="ref2" /> | | | 5'-TCTAGACGGCAGGTCAGGTCACC-3'<ref name="ref2" /> | ||
} | } | ||
| + | |||
===Allelic Information and Variation=== | ===Allelic Information and Variation=== | ||
Please input allelic information and variation information here. | Please input allelic information and variation information here. | ||
Revision as of 07:42, 8 May 2015
TDRG1, a developmentaly related testicular-specific gene, which was located in chromosome 6p21.1-p21.2 with 1197 bp in length, may involve in normal human spermatogenesis and fertilization.
Contents
Annotated Information
Transcriptomic Nomeclature
TDRG1:testis development related 1 (non-protein coding)(HGNC nomenclature）
characteristics
TDRG1 was 1197 bp in length, located in chromosome 6p21.1-p21.2. The sequence of the open reading frame was 504-806bp. The cDNA encodes a novel protein of 100 amino acids. TDRG1 is exclusively expressed in the testis but not in any other non-reproductive tissues. TDRG1 is mainly located in spermatogenic cells in seminiferous tubules of adult testis[1].
Function
G1 is a human testis-specific gene and may play important roles in normal human spermatogenesis[1].
Expression
TDRG1 is specially expressed in spermatogenic cells, with highest expression level during human puberty stage, and decresing levels of expression with aging[1]. Primers for regular RT-PCR and real time RT-PCR were as follows,
| Primers | RT-PCR | real time RT-PCR | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TDRG1 | 5'-AAGAGGAGGGAGGCAGTCT-3' | 5'-GCCCAATTCCTCTTGACTGA-3'[1] | ||||||||||||||||||||
| GAPDH | 5'-CCACCCATGGCAAATTCCATGGCA-3' | 5'-TCTAGACGGCAGGTCAGGTCACC-3'[2]
} Allelic Information and VariationPlease input allelic information and variation information here. EvolutionPlease input evolution information here. You can also add sub-section(s) at will. Labs working on this lncRNA
References
Basic Information
|
