Difference between revisions of "MDC1-AS1"

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25514464
 
25514464
 
===Cellular Localization===
 
===Cellular Localization===
−
MDC1-AS and MDC1 were exist primarily in the nuclear fraction <ref name="ref1" />.
+
MDC1-AS primarily exists in the nuclear fraction <ref name="ref1" />.
  
 
===Function===
 
===Function===
 +
MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.<ref name="ref1" />
  
−
MDC1-AS, which may participate in bladder cancer through up-regulation of its antisense tumor-suppressing gene MDC1 <ref name="ref1" />
+
MDC1-AS exhibits significant decreases in proliferation,colony formation, migration and invasion.<ref name="ref1" />
 +
 
 +
===Diseases===
 +
MDC1-AS may act as a tumor suppressor in bladder cancer <ref name="ref1" />.
  
 
===Expression===
 
===Expression===
−
[[File:Dysregulation of MDC1-AS and MDC1 expression level.jpg|right|thumb|400px|''Dysregulation of MDC1-AS and MDC1 expression level''' <ref name="ref1" />.]]  
+
[[File:Dysregulation of MDC1-AS and MDC1 expression level.jpg|right|thumb|600px|''(A) MDC1-AS levels in cancerous tissues were significantly lower than those in the noncancerous tissues. (B) MDC1 was down-regulated in bladder cancerous tissues, compared with that in adjacent normal tissues. (C) Expression levels of MDC1-AS and MDC1 in tissues were significantly correlated in a positive direction. (D) Both MDC1-AS and MDC1 were decreased in bladder cancer cells T24 and EJ, compared with those in normal cell line 293A.''' <ref name="ref1" />.]]  
  
−
The expression levels of MDC1-AS was down-regulated in bladder cancer <ref name="ref1" />.
+
MDC1-AS levels in cancerous tissues are evidently lower than those in the noncancerous tissues <ref name="ref1" />.
 +
 
 +
MDC1 is down-regulated in bladder cancer tissues, which was concordant with MDC1-AS <ref name="ref1" />.
 +
 
 +
Expression levels of MDC1-AS and MDC1 in tissues are significantly correlated in a positive direction, indicating a potential regulating role of MDC1-AS on the coding gene MDC1 <ref name="ref1" />.
 +
 
 +
The expression levels of MDC1-AS and MDC1 are decreased in bladder cancer cells <ref name="ref1" />.
  
−
There is a remarkably inhibitory role of antisense lncRNA MDC1-AS on malignant cell behaviors in bladder cancer cells EJ and T24, which knockdown of MDC1 revealed that suppressing role of MDC1-AS was attributed to up-regulation of MDC1 <ref name="ref1" />.
 
  
 
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−
===Diseases===
+
 
−
MDC1-AS may act as a tumor suppressor in bladder cancer <ref name="ref1" />.
+
 
 +
 
 +
 
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
−
* Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China
+
* Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer
 +
Center, Nanjing Medical University, Nanjing, China
  
 
==References==
 
==References==
 
<references>
 
<references>
−
<ref name="ref1">Ji, P., Diederichs, S., Wang, W., Boing, S., Metzger, R., Schneider, P.M., Tidow, N., Brandt, B., Buerger, H., Bulk, E. et al. (2003) MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene, 22, 8031-8041.</ref>
+
<ref name="ref1">Xue, Y., G. Ma, Z. Zhang, Q. Hua, H. Chu, N. Tong, L. Yuan, C. Qin, C. Yin, and M. Wang. "A Novel Antisense Long Noncoding Rna Regulates the Expression of Mdc1 in Bladder Cancer." Oncotarget 6, no. 1 (2015): 484-93.</ref>
−
 
 
 
</references>
 
</references>
  

Latest revision as of 08:27, 19 September 2016

Annotated Information

Approved Symbol

MDC1-AS1

Approved Name

MDC1 antisense RNA 1

Synonyms

MDC1-AS

Chromosome

6p21.33

RefSeq ID

NR_133647

Ensembl ID

ENSG00000224328

pubmed IDs

25514464

Cellular Localization

MDC1-AS primarily exists in the nuclear fraction [1].

Function

MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.[1]

MDC1-AS exhibits significant decreases in proliferation,colony formation, migration and invasion.[1]

Diseases

MDC1-AS may act as a tumor suppressor in bladder cancer [1].

Expression

(A) MDC1-AS levels in cancerous tissues were significantly lower than those in the noncancerous tissues. (B) MDC1 was down-regulated in bladder cancerous tissues, compared with that in adjacent normal tissues. (C) Expression levels of MDC1-AS and MDC1 in tissues were significantly correlated in a positive direction. (D) Both MDC1-AS and MDC1 were decreased in bladder cancer cells T24 and EJ, compared with those in normal cell line 293A.' [1].

MDC1-AS levels in cancerous tissues are evidently lower than those in the noncancerous tissues [1].

MDC1 is down-regulated in bladder cancer tissues, which was concordant with MDC1-AS [1].

Expression levels of MDC1-AS and MDC1 in tissues are significantly correlated in a positive direction, indicating a potential regulating role of MDC1-AS on the coding gene MDC1 [1].

The expression levels of MDC1-AS and MDC1 are decreased in bladder cancer cells [1].


Experiment Primer Forward primer Reverse primer
RT-PCR MDC1-AS 5'-TCCCAGATGTGCCAAAGTCAG-3' 5'-AGCAACCCCAGTTGTCATTC-3'[1]
MDC1 5'-GCAGCTTCCAGACAACAG-3' 5'-TACCCATGACTTTATCCACA-3'[1]
GAPDH 5'-AAGGTGAAGGTCGGAGTCAAC-3' 5'-GGGGTCATTGATGGCAACAATA-3' [1]
RNAi siMDC1 5'-GCGGCUACAUAUCUUUAGUTT-3' 5'-ACUAAAGAUAUGUAGCCGCTT-3'[1]




Labs working on this lncRNA

  • Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer

Center, Nanjing Medical University, Nanjing, China

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Xue, Y., G. Ma, Z. Zhang, Q. Hua, H. Chu, N. Tong, L. Yuan, C. Qin, C. Yin, and M. Wang. "A Novel Antisense Long Noncoding Rna Regulates the Expression of Mdc1 in Bladder Cancer." Oncotarget 6, no. 1 (2015): 484-93.

Sequence

>gi|949474747|ref|NR_133647.1| Homo sapiens MDC1 antisense RNA 1 (MDC1-AS1), long non-coding RNA

000001 CACTATATCG GTCTGTCATC ATCCCTTGGC CTCTCACCTG ATGCAGCCAG TCCAGGGACA GAATGGGGAT TCCCCGCCCC 000080
000081 AGGGCACACA GGAACTTGAC TGTCCGGCGG ATGCGATCAG TGACCAGACA CCTGATGCTT CTTTATATGT ATAATGGCTG 000160
000161 ACCCTGGCGG GACTTCCTTC TCCACTTGTG TGTTGATGTC CACTGTGGTG GAGGCTTGGC TTCTCTCCAG GTGGATCCCA 000240
000241 GATGTGCCAA AGTCAGCGCT GCTGAGACTT CTTCCTCGTC ATCTGTATCG CTGTTGATAA CCATGGAAGC TTGGCTTTTC 000320
000321 TCCAGAGGGA CAGCCTGTGG GGCCTTGCCT TCTTCCACAT CTGTATCACT ACCAGCCTGG CTCTCCTGCA GATGGGCCAG 000400
000401 GCCTGGTGCT CCAGGACCCC TTGTACCTAC TCCATGGAAG ATCTTCCTCT TCTTCATAGG AATGACAACT GGGGTTGCTG 000480
000481 GGATCCTCTC TTCTTCCGCA TCAGTGTCGC TGTCGATGAA GCCAAAAGGC TGAGCCCTTT CCAAATGGAC CTCAGCTGGC 000560
000561 CTTCCAGGAG GCCTGCTGTC ATCATCCACA TCTGTGTCAC TGTCCTCTCC AGGAGGTTGG CTCCTCTCCA GAATCACCCC 000640
000641 AGCTGGAACC ACCCCATTCC CTGCACCCCT CTTGACTTTT GTATCATTGT CCCTCTCCTT CACTAAAGGC TGATCCTTTT 000720
000721 CAAGCTGGAT TTCAGTTA

Predicted Small Protein

Name MDC1-AS1_smProtein_302:532
Length 76
Molecular weight 7927.2718
Aromaticity 0.105263157895
Instability index 48.4697368421
Isoelectric point 10.3208618164
Runs 14
Runs residual 0.0428009679371
Runs probability 0.0466033260151
Amino acid sequence MEAWLFSRGTACGALPSSTSVSLPAWLSCRWARPGAPGPLVPTPWKIFLFFIGMTTGVAG
ILSSSASVSLSMKPKG
Secondary structure LEEEEELLLEELLLLLLLLLLLLHHHHLLLLLLLLLLLLLLLLLLEEEEEEELLLLLHHH
EEELLLEEEEEEELLL
PRMN LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLHHHHHHHHHHHHHHHHHH
HHLLLLLLLLLLLLLL
PiMo ooooooooooooooooooooooooooooooooooooooooooTTTTTTTTTTTTTTTTTT
TTiiiiiiiiiiiiii
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