Difference between revisions of "PINCR"

Revision as of 08:13, 1 August 2017

Annotated Information

Name

PINCR:p53-induced noncoding RNA

Characteristics

PINCR is a ~1.8 kb noncoding RNA that is transcribed from cheomosome X (Xp11.3) ^[1].

Cellular Localization

PINCR is highly enriched in the nucleus ^[1].

Function

PINCR is induced ~100-fold after DNA damage and exerts a prosurvival function in human colorectal cancer cells (CRC) in vitro and tumor growth in vivo ^[1]. PINCR regulates a subset of p53 targets involved in G1 arrest and apoptosis (BTG2, RRM2B and GPX1) in human colorectal cancer cells by binding to Matrin 3, and plays an important role in p53-dependent G1 arrest ^[1].

Regulation

PINCR promoter contains p53RE, and PINCR is a direct target of p53 ^[1].

Diseases

colorectal cancer ^[1]

Expression

PINCR is induced ~100-fold after DNA damage ^[1].

Conservation

Mature PINCR transcript and the transcription start site are quite conserved among primates but poorly conserved between human and mouse ^[1].

Sequence

>NR_110387.1 Homo sapiens p53-induced noncoding RNA (PINCR), long non-coding RNA

000001  ATTCAATCTG TGAGGTGGAT GCGTGTTGGA AGGACCCCTT TTGGTTTCTT TTTGCTCAGA GCTTTCTTTT AATAAATTCC  000080
000081  GCTCTCCTCA CCTTTCAATG TGTCCGTATG CCTAATTTTT CCTGGTCCTG TGACAAGAAC CTGATTTTAG CTGAACTAAG  000160
000161  GAGCGAAAGA TCCTGGATCA TTTTGGTGAC CCATACTGGT ACATGAGGAA AGCTCCTATT CCAGATTCTC AGAGTATAGA  000240
000241  TTCTGACTGG ATTACCCTGG TCCTTGAAGT ATACCTAGGA GATGGAATAA CATTGTATAA GCATCCACGT TGTCATTAAG  000320
000321  AGGAAAGAGT TTCCATTAAA ACAGCAGGTC ACAAAACCCT CCTGTGAGAG GAACCCTCTG TGGAAGAACA GAATATGTCA  000400
000401  CCCCAAAATA TGAAGAATTG TTGAGCTGAA GATGATTAAG AAGAAGCGGA TGCAGGAAAG CTCTCTGCTC TCCCTCTATT  000480
000481  TGCTTAAAAG CAGGATAAAC ACTTACAAAG ACTAAAGACG CCACAATCAA GAACTCAGAA GGGTAGAAGA AAAAGATAGT  000560
000561  TTCCCTTCCC TACACAGGTG TTGATCCCTT CATAAATATT ATGCATGCTA AGCCCTATCT CAAGGTCTAC ATCCAGGAGA  000640
000641  ACCCAACCTG CAACATTAAG TTACTGACTG TGCAAGAACC CTTTGATCAC GTACCTTACA GACCAAGATA AGTGAGGATG  000720
000721  CCCATGTATA ACCTGCTGTC TTTCTGGTCT CTTATGTTTT TGTACCAGAA AGGGACAACT TTTTGGGTAA CCATAGGTCT  000800
000801  CTAGTATATC TGTGATTACA ATAAAGATTT TTGATATACC AAAACTGATG AACCAAAATT CCACTGAAAT AGCGAGTAAA  000880
000881  TGGAGTTCTT TTATCCTTTG CAGTATACCA GGGTGAACTT TTTACATGAT TTCTTGCTCC AAAGGGAGAA GTGTTATGAC  000960
000961  AAAGTACATT TAAGGACCCT AACTGATCAC AGAAAAAATA TTAATGGCAT TGGGAAAAAA ATGCAGACTA ATGAATCCAC  001040
001041  TACTAAATTT TATCTATCTT GTAGCTCTAA AATTGCACTG TTTAGAATAC ACTATGGTAG CCACTAGACA TATGGCTACT  001120
001121  TGAACTTGAA TTGGTTAAAA TCAAAATTAA GAATTTAATT TCTCACTCAC ATTAGCCACA TTTCAAGTGC TCAACAGACA  001200
001201  CATGTGGCTA GTGTCTACCA TATTGGACAG CATAGATTTA AAGCACATTT CCATCATTGC AACACTGCAA TATGGAGGAT  001280
001281  GTATCCTCAA TACATTGATA TTGCCCCCTC AAAATTCATA ACCCCTAGAT ATCTTATCTC TGTATGTGAG GATAGTGTAT  001360
001361  TAACTTGCAC CTGGACTGAG ATAGATAAAT GCTTATCTCC AGAGATGTCT GAAAGTCTGG TACTGACCCT TGTCTAAGAT  001440
001441  AAGGATGGAA TGTGCTGGTT AATGATTTAT ACTGTGCTAT ACAAAGTCCA AAATTCCTTT GGAGGTATCC TCTGCTGGCT  001520
001521  TGTCTACATT GTTTAAGGAT GGATAGGTTG ATGGTTTGCA CCTGCTTTAA TCATTTACAC ATTCTGATTT TATTTGTGAT  001600
001601  CAGTGGAGGA TAAAACCTTC AATAAACTTG TGTCTTAGCT TCCTTGATAT TAAGATATCT TGCACATATC TTAGATTTTT  001680
001681  ATAATCTGAA TGCAAAGCAC ATCCTTTGCA ATTGAGAAAG GACTCTGTGA GCTGCTCCTG GATATGTGAA ACCCCTCAGT  001760
001761  GACTGACCGT ATTTTCTTTC TTCTGCTGTA CTTGCCCTTA TTAAAGCCTT ACATGTGAAG TAAGTCCTTT CAATTGTCTG  001840
001841  ACTCCGGGTG ATTGTTATAC CCTTTGATAT AGTTT

[1]

@@ Line 2: / Line 2: @@
 ===Name===
 PINCR:p53-induced noncoding RNA
 ===Characteristics===
-PINCR is a noncoding RNA ; highly enriched in the nucleus , similar to the nuclear-retained lncRNA MALAT1 (Hutchinson et al., 2007); the 5’and 3’ends of PINCR match the annotated transcript based on analysis of our RNA-seq data from HCT116 cells (Li et al., unpublished); analysis of the length of the PINCR transcript by RT-PCR reveals two closely migrating bands that match the expected size of the amplicon (~1.8 kb); PINCR promoter including the p53RE, mature PINCR transcript and the transcription start site are quite conserved among primates but poorly conserved between human and mouse.<ref name="ref1" />
+PINCR is a ~1.8 kb noncoding RNA that is transcribed from cheomosome X (Xp11.3) <ref name="ref1" />.
 ===Cellular Localization===
-Xp11.3
+PINCR is highly enriched in the nucleus <ref name="ref1" />.
 ===Function===
-PINCR plays a role in p53-dependent G1 arrest and it has a prosurvival function in response to DNA damage. PINCR loss results in hypersensitivity to 5-FU and decreases tumor growth. PINCR regulates the induction of a subset of p53 targets after DNA damage.
+PINCR is induced ~100-fold after DNA damage and exerts a prosurvival function in human colorectal cancer cells (CRC) in vitro and tumor growth in vivo <ref name="ref1" />. PINCR regulates a subset of p53 targets involved in G1 arrest and apoptosis (BTG2, RRM2B and GPX1) in human colorectal cancer cells by binding to Matrin 3, and plays an important role in p53-dependent G1 arrest <ref name="ref1" />.
-RNA pulldowns and mass spectrometry identifies Matrin 3 as a PINCR interacting protein that mediates the effect of PINCR.
-Matrin 3 associates with enhancers within insulated neighborhoods of PINCR targets.
-PINCR associates with the enhancer regions of select PINCR targets via Matrin 3<ref name="ref1" />
 ===Regulation===
-PINCR is a direct target of p53.<ref name="ref1" />
+PINCR promoter contains p53RE, and PINCR is a direct target of p53 <ref name="ref1" />.
 ===Diseases===
 colorectal cancer <ref name="ref1" />
 ===Expression===
-PINCR is expressed at ~13–26 molecules per HCT116 cell after DNA damage and less than one molecule per cell without DNA damage based on comparison of the FPKM of PINCR with the lncRNA NORAD, known to be expressed at 500–1000 molecules per HCT116 cell (Lee et al., 2016). As an alternative approach, qRT-PCR using in vitro transcribed PINCR RNA showed that PINCR is expressed at ~27 molecules per HCT116 cell after DNA damage. <ref name="ref1" />
+PINCR is induced ~100-fold after DNA damage <ref name="ref1" />.
+===Conservation===
+Mature PINCR transcript and the transcription start site are quite conserved among primates but poorly conserved between human and mouse <ref name="ref1" />.
 ===Sequence===