Difference between revisions of "JADRR"

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(Created page with "JADRR is a key functional link that connects the DDR to histone H4 acetylation and play a potential role in breast tumorigenesis. ==Annotated Information== ===Name=== JADRR:...")
 
 
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===Characteristics===
 
===Characteristics===
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LncRNA-JADE,an independent transcript which contains no overlapping sequence with the transcripts from its neighbouring genes, is transcribed in the opposite orientation to the Jade1 gene, while its human homologue has the same orientation as Jade1.LncRNA-JADE and Jade1 genes have distinct promoters at the chromosomal region 3qB in mouse<ref name="ref1" />.  
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JADRR,an independent transcript which contains no overlapping sequence with the transcripts from its neighbouring genes, is transcribed in the opposite orientation to the Jade1 gene in mouse, while its human homologue has the same orientation as Jade1 <ref name="ref1" />.  
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JADRR and Jade1 genes have distinct promoters at the chromosomal region 3qB in mouse <ref name="ref1" />.  
  
 
===Function===
 
===Function===
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JADRR is a key functional link that connects the DNA damage response (DDR) to histone H4 acetylation, and that dysregulation of JADRR may contribute to breast tumorigenesis <ref name="ref1" />. JADRR transcriptionally activates Jade1, a key component in the HBO1 (human acetylase binding to ORC1) histone acetylation complex. Consequently, JADRR induces histone H4 acetylation in the DDR <ref name="ref1" />.
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LncRNA-JADE as an important link that connects the DNA damage signalling to the Jade1-mediated H4 acetylation<ref name="ref1" />.
 
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LncRNA-JADE may supress the DNA damage-induced apoptosis and decrease the sensitizition of cells to DNA damaging agents<ref name="ref1" />.
 
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LncRNA-JADE promotes cell proliferation, inhibits DNA damage checkpoints, and reduces the cellular sensitivity to DNA damaging drugs, suggesting that lncRNA-JADE possibly has the proto-oncogene properties<ref name="ref1" />.
 
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===Regulation===
 
===Regulation===
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The transactivation of lncRNA-JADE is regulated by the ATM-NF-κB signalling in the DDR <ref name="ref1" />
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The transactivation of JADRR is regulated by the ATM-NF-κB signalling in the DDR <ref name="ref1" />
  
 
===Diseases===
 
===Diseases===
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===Expression===
 
===Expression===
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lncRNA-JADE showed very low or undetectable expression levels in all of normal breast tissues. However, breast tumours exhibited overexpressed lncRNA-JADE, some tumour samples even had high or very high levels of lncRNA-JADE<ref name="ref1" />.
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JADRR shows very low or undetectable expression levels in all of normal breast tissues. However, breast tumours exhibits overexpressed JADRR, and some tumour samples even show high or very high levels of JADRR <ref name="ref1" />.
  
 
The RT–PCR primers are shown below:
 
The RT–PCR primers are shown below:

Latest revision as of 09:01, 1 August 2017

JADRR is a key functional link that connects the DDR to histone H4 acetylation and play a potential role in breast tumorigenesis.

Annotated Information

Name

JADRR: JADE1 adjacent regulatory RNA (HGNC nomenclature)

INXS:lncRNA-JADE [1]

Characteristics

JADRR,an independent transcript which contains no overlapping sequence with the transcripts from its neighbouring genes, is transcribed in the opposite orientation to the Jade1 gene in mouse, while its human homologue has the same orientation as Jade1 [1].

JADRR and Jade1 genes have distinct promoters at the chromosomal region 3qB in mouse [1].

Function

JADRR is a key functional link that connects the DNA damage response (DDR) to histone H4 acetylation, and that dysregulation of JADRR may contribute to breast tumorigenesis [1]. JADRR transcriptionally activates Jade1, a key component in the HBO1 (human acetylase binding to ORC1) histone acetylation complex. Consequently, JADRR induces histone H4 acetylation in the DDR [1].

Regulation

The transactivation of JADRR is regulated by the ATM-NF-κB signalling in the DDR [1]

Diseases

  • Breast cancer[1]

Expression

JADRR shows very low or undetectable expression levels in all of normal breast tissues. However, breast tumours exhibits overexpressed JADRR, and some tumour samples even show high or very high levels of JADRR [1].

The RT–PCR primers are shown below:

Primer Forward primer Reverse primer
Mouse lncRNA-JADE 5'-CCCCCTCCAGGTACCAGAAC-3' 5'-TTCACTGTGGACCCGGTGATTAAC-3'[1]
Mouse Jade 5'-AGCTTGTCAACGACATGGTCC-3' 5'-AGGCTTTCGATCTTCAGGTCT-3'[1]
Human lncRNA-JADE 5'-AACCTGAAGAGAGCGT-3' 5'-AGGAAGCGAATGCTCA-3'[1]
Human Jade1 5'-GCAGACCAGCATGACAGATTT-3' 5'-GGATTAGGGCTTCCTCTTGGA-3'[1]
Human IRF-1 5'-TCTTAGCATCTCGGCTGGACTTC-3' 5'-CGATACAAAGCAGGGGAAAAGG-3'[1]

Sequence

>gi|478437780|gb|KC469579.1| Homo sapiens lncRNA-JADE, complete sequence

000001 GCAGACTTTT AGAAGAACTA ACTTCAAAAA AGATTCCCTA TATAATAAGA GTCATTTTAA ACTGAAATGA TCAAGACCTC 000080
000081 CTAGGGATTC TAGGTCATGC CTAAACCATC ACGGTCAAGT TGGACATGTA TAGAGACCTG GTACTCTCAC TCTTCCACCT 000160
000161 TCGGCCCCAC TTATATCCCA GGCTGGGCAT TGAAGTTCCC CATCAGTCAG TTGGACGGGG GTACAGGACA GGCCCCTTTA 000240
000241 AACGCAGAAC TCCCTCAATA CTGCAGAACA CTGGAAGAGC TCTTGTTCAA GTGGGTAAGT GCAGCCTGAG TGTCTGGTGA 000320
000321 GATCAAACCT GAAGAGAGCG TGAGATAAGG AGAGAGTTGA GTCATTAGTC ATCTCTTTTA ACTAACTCTC TGAAGTTGGC 000400
000401 TGGCTTCACA CTAGACCTGG TGATTAATGG TAAAGAGATA GCTCTTGCAA TTCAACTTCT GTAAGTAGAA AGAAATTTAA 000480
000481 AGCTTCCCCT GAGCATTCGC TTCCTCTTCG AATGCTTCAA AACAAATCTC AGCATCAGTA GGCTGATGCC AGTTCTCATC 000560
000561 ATGCCCCACA GGCCTTGATG AGGGAGACGG AGAGAGCCAG GTGTGCCCTA TTGAACAGGC CCCAATGGTT CTCTGCCTTT 000640
000641 TCATAGCAAT CTCCTAAAGG GAGAACCAGA GTCATCAAAT CTCAGGACAA AATGGCAGCC CTAAGAGGGC AATCCACTGT 000720
000721 CAGAAAGCAT GCATAAGCTA TTATTCTGAA GAGAAGAAGA TTATTTCCAT TCACTATAGG GCTAGTCTGA AGTTTGCAAA 000800
000801 GAAGTCCTTT TCATTCAATT ATCCCTTGAA GGAAAGTATG TATGTAAAAG TTAAGAAAAT TCAATATCAA AAATAGTTTT 000880
000881 TGTTTTGGCT TTGAAATTTT TCTTAATATG TAAGCAAAAT TCTTAGTTCT GGAGTTGCAG TTACTCAGTT ATCAAAAATG 000960
000961 ATACAGATAA GATAACAAAC CCCATTAACC ACCCAGTTGC TCAAACAGAA ATCTAGGATT CATGTTAATT CCTTTCTTCT 001040
001041 TTCACCCCTC ACACCCAATC CATTTGTTTC ATTAGTTCTC CCTTCAAAAT ACCTTAATTC ATCCACTTTC TCCATTCTCA 001120
001121 TGGCACCATT CTAATATAAA TCATGTCTCA CCTGAATTGT TATCATAACC TCTTAAACAG AACTAACTCT TGCTTCCATA 001200
001201 GTCTATTTCA TGGCAGCCAG AGAGAGATTT TTAAAACACC CATAAGATCT GTCCAGGCAT GGTGGCTCAT GCCTGTAATC 001280
001281 CCAGCACTTT GGGAGGCTGA GGCGGGTGGA TTGCCTGAGG TTAGGAGTTC GAGACCAGCC TGGCCAACAT AGTAAAACCC 001360
001361 TGTCTCTACT AAAAATACAA AAAATTAGCT GGGCGTGGTG ACAGGTGCCT GTAATCCCAG CTACTATGGA GGCTGAGGCA 001440
001441 GGAGAATTGC TTGAACCTGG GAGGCGGAGG TTGCAGTGAG CTGAGATCGC TCCATTGCTC TCCAGCCTGG GCAACAAGAG 001520
001521 TGAAACTCTG TCTGAAAAAC AAAAAACAAA AAAATTGGCC AGGCGTGGTG ATGCATGCCT GTAATTCCAG CTACTAGGGA 001600
001601 GGCTGAAGCA GGAGAATTGC TTGAACCCAG GAGGTGGAGG TTGCAGTGAG CCGAGATGGT GCCACTGCAC TCCAGCCTGG 001680
001681 GTGACAGAGC AAGGCTCTGT CTCAAAAAAA AAAAAAAAAA A

Labs working on this lncRNA

  • Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. [1].

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Wan G, Hu X, Liu Y, Han C, Sood AK, Calin GA, et al. A novel non-coding RNA lncRNA-JADE connects DNA damage signalling to histone H4 acetylation[J]. The EMBO journal. 2013,32(21):2833-47.
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