Difference between revisions of "BOK-AS1"
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==Annotated Information== | ==Annotated Information== | ||
===Approved Symbol=== | ===Approved Symbol=== | ||
| − | BOK-AS1 | + | BOK-AS1 (BOK antisense RNA 1) |
| − | |||
| − | BOK antisense RNA 1 | ||
===Previous Symbols=== | ===Previous Symbols=== | ||
BOKAS | BOKAS | ||
| + | [[File:Schematic representation of genomic organization of BOKAS in the relation with hBok.png|right|thumb|400px|'''Schematic representation of genomic organization of BOKAS in the relation with hBok''' <ref name="ref1" />.]] | ||
===Synonyms=== | ===Synonyms=== | ||
"NCRNA00151, NAToB" | "NCRNA00151, NAToB" | ||
| Line 12: | Line 11: | ||
===RefSeq ID=== | ===RefSeq ID=== | ||
NR_033346 | NR_033346 | ||
| − | |||
| − | |||
===Ensembl ID=== | ===Ensembl ID=== | ||
ENSG00000234235 | ENSG00000234235 | ||
| − | === | + | ===Characteristics=== |
| − | + | The BOK-AS1 gene consists of 2 exons and it is a natural antisense transcript of the hBok gene.<ref name="ref1" /> | |
| + | The full-length BOK-AS1 transcript has 855 nucleotides but does not contain a long open reading frame, giving 79 amino acids in conceptually translation.<ref name="ref1" /> | ||
| + | |||
| + | ===Function=== | ||
| + | BOK-AS1 function specifically in the human testis, where it serves as an antisense molecule to regulate Bok-induced apoptosis.<ref name="ref1" /> | ||
| + | Overexpression of BOK-AS1 reduced Bok-induced cell death. To assess the effect of BOK-AS1 on Bok-induced apoptosis, overexpressed ectopically BOK-AS1 in HeLa cells showed expression of BOK-AS1 alone exhibited no significant anti- or pro-apoptotic activity when compared with control plasmids.<ref name="ref1" /> | ||
| + | |||
| + | ===Expression=== | ||
| + | BOK-AS1 mRNA is differentially expressed in human tissues.<ref name="ref1" /> | ||
| + | The mRNA expression of BOK-AS1 is only detected in testis and certain cancer tissues but not in other normal adult tissues examined.<ref name="ref1" /> | ||
| + | |||
| + | {| class='wikitable' style="text-align:center" | ||
| + | |- | ||
| + | ! | Experiment | ||
| + | ! | Forward primer | ||
| + | ! | Reverse primer | ||
| + | |- | ||
| + | | rowspan="1"|Quantitative PCR | ||
| + | | | 5'-GTTCTGGCTTCAGGATCTGC-3' | ||
| + | | | 5'-TCCAAAATAGGCCTCAGTGG-3'<ref name="ref1" /> | ||
| + | |} | ||
| + | |||
===Sequence=== | ===Sequence=== | ||
>gi|289629233|ref|NR_033346.1| Homo sapiens BOK antisense RNA 1 (BOK-AS1), antisense RNA | >gi|289629233|ref|NR_033346.1| Homo sapiens BOK antisense RNA 1 (BOK-AS1), antisense RNA | ||
<dnaseq>CCCCACGGCGACTGCCAGGACCCGGCCCTCCCCGCCCCGGCCCGGGGCCGCCCACCTCCGCCGCTGGCGTCCTGCGCGCGTGTCCCCGGCCTCGCCGTTCCCGTGGCACCCCGGGCACCCCGGCACGGACGCCACGCCCGCCGCGCTCACCTCTCCCGCCGCATGGCGCGGGCGCCGGGGTGGAGTTCTGGCTTCAGGATCTGCTCGCCGCCGACGACGACCTCGACCCTTCCATTCAGACCCGCGAGGACGCGCGCCCCGCCCCGGCGCCCGCCCCGTCCACGCCCGAGCCGGGAGCGCCAGGTCCACGGGGCTTCCCGCGCTCTTGGGGGGCTCCTGCCGCGGCGTCGGGGGCCTGGGCAGCGAGGGCCTCCAGAGGAACCAGCGCTGCCGACTCCTTGATTCTAACCCACTGAGGCCTATTTTGGATTTCTGCACCCCAGAACTGTGGGAGAGTAAATTGTGCTGTTGAAGCCACCACATTTGTGGTGATCTGTTCCAGCTGCAGTAGGAAGCTCGTATGCTTGGCAGTTCTGATTGTGAACTCACCTTTATGTTGGAGATTCCTTTACACATAAGTCCCCTTATCCACAGCGGACAAATTCCATGACCCCCAGTGGATGCCTGAAATCGCGGAGAGTACTGAATCCTACATATAATGTGTTCTTTCCTATATGTATATGTATATATCTATATCTATGATACAGTCTAATTTATAAATTAGGCACAGTGAGAGATTAACAACAATAACTAATAGTAAAATAGGACAATGATGACAATATAATGTAATAAAAGTTTTGTGAATATGGTCTCTCTTCTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAA</dnaseq> | <dnaseq>CCCCACGGCGACTGCCAGGACCCGGCCCTCCCCGCCCCGGCCCGGGGCCGCCCACCTCCGCCGCTGGCGTCCTGCGCGCGTGTCCCCGGCCTCGCCGTTCCCGTGGCACCCCGGGCACCCCGGCACGGACGCCACGCCCGCCGCGCTCACCTCTCCCGCCGCATGGCGCGGGCGCCGGGGTGGAGTTCTGGCTTCAGGATCTGCTCGCCGCCGACGACGACCTCGACCCTTCCATTCAGACCCGCGAGGACGCGCGCCCCGCCCCGGCGCCCGCCCCGTCCACGCCCGAGCCGGGAGCGCCAGGTCCACGGGGCTTCCCGCGCTCTTGGGGGGCTCCTGCCGCGGCGTCGGGGGCCTGGGCAGCGAGGGCCTCCAGAGGAACCAGCGCTGCCGACTCCTTGATTCTAACCCACTGAGGCCTATTTTGGATTTCTGCACCCCAGAACTGTGGGAGAGTAAATTGTGCTGTTGAAGCCACCACATTTGTGGTGATCTGTTCCAGCTGCAGTAGGAAGCTCGTATGCTTGGCAGTTCTGATTGTGAACTCACCTTTATGTTGGAGATTCCTTTACACATAAGTCCCCTTATCCACAGCGGACAAATTCCATGACCCCCAGTGGATGCCTGAAATCGCGGAGAGTACTGAATCCTACATATAATGTGTTCTTTCCTATATGTATATGTATATATCTATATCTATGATACAGTCTAATTTATAAATTAGGCACAGTGAGAGATTAACAACAATAACTAATAGTAAAATAGGACAATGATGACAATATAATGTAATAAAAGTTTTGTGAATATGGTCTCTCTTCTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAA</dnaseq> | ||
| + | |||
| + | ==Labs working on this lncRNA== | ||
| + | * University Women's Hospital Basel and Research Department of University Hospital, CH-4031 Basel, Switzerland.<ref name="ref1" /> | ||
| + | |||
| + | ==References== | ||
| + | <references> | ||
| + | <ref name="ref1"> Zhang H, Gao S, De Geyter C. A natural antisense transcript, BOKAS, regulates | ||
| + | the pro-apoptotic activity of human Bok. Int J Oncol. 2009 Apr;34(4):1135-8. | ||
| + | </ref>(1) | ||
| + | </references> | ||
Latest revision as of 14:42, 8 December 2017
Contents
Annotated Information
Approved Symbol
BOK-AS1 (BOK antisense RNA 1)
Previous Symbols
BOKAS
Schematic representation of genomic organization of BOKAS in the relation with hBok [1].
Synonyms
"NCRNA00151, NAToB"
Chromosome
2q37.3
RefSeq ID
NR_033346
Ensembl ID
ENSG00000234235
Characteristics
The BOK-AS1 gene consists of 2 exons and it is a natural antisense transcript of the hBok gene.[1] The full-length BOK-AS1 transcript has 855 nucleotides but does not contain a long open reading frame, giving 79 amino acids in conceptually translation.[1]
Function
BOK-AS1 function specifically in the human testis, where it serves as an antisense molecule to regulate Bok-induced apoptosis.[1] Overexpression of BOK-AS1 reduced Bok-induced cell death. To assess the effect of BOK-AS1 on Bok-induced apoptosis, overexpressed ectopically BOK-AS1 in HeLa cells showed expression of BOK-AS1 alone exhibited no significant anti- or pro-apoptotic activity when compared with control plasmids.[1]
Expression
BOK-AS1 mRNA is differentially expressed in human tissues.[1] The mRNA expression of BOK-AS1 is only detected in testis and certain cancer tissues but not in other normal adult tissues examined.[1]
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| Quantitative PCR | 5'-GTTCTGGCTTCAGGATCTGC-3' | 5'-TCCAAAATAGGCCTCAGTGG-3'[1] |
Sequence
>gi|289629233|ref|NR_033346.1| Homo sapiens BOK antisense RNA 1 (BOK-AS1), antisense RNA
000001 CCCCACGGCG ACTGCCAGGA CCCGGCCCTC CCCGCCCCGG CCCGGGGCCG CCCACCTCCG CCGCTGGCGT CCTGCGCGCG 000080
000081 TGTCCCCGGC CTCGCCGTTC CCGTGGCACC CCGGGCACCC CGGCACGGAC GCCACGCCCG CCGCGCTCAC CTCTCCCGCC 000160
000161 GCATGGCGCG GGCGCCGGGG TGGAGTTCTG GCTTCAGGAT CTGCTCGCCG CCGACGACGA CCTCGACCCT TCCATTCAGA 000240
000241 CCCGCGAGGA CGCGCGCCCC GCCCCGGCGC CCGCCCCGTC CACGCCCGAG CCGGGAGCGC CAGGTCCACG GGGCTTCCCG 000320
000321 CGCTCTTGGG GGGCTCCTGC CGCGGCGTCG GGGGCCTGGG CAGCGAGGGC CTCCAGAGGA ACCAGCGCTG CCGACTCCTT 000400
000401 GATTCTAACC CACTGAGGCC TATTTTGGAT TTCTGCACCC CAGAACTGTG GGAGAGTAAA TTGTGCTGTT GAAGCCACCA 000480
000481 CATTTGTGGT GATCTGTTCC AGCTGCAGTA GGAAGCTCGT ATGCTTGGCA GTTCTGATTG TGAACTCACC TTTATGTTGG 000560
000561 AGATTCCTTT ACACATAAGT CCCCTTATCC ACAGCGGACA AATTCCATGA CCCCCAGTGG ATGCCTGAAA TCGCGGAGAG 000640
000641 TACTGAATCC TACATATAAT GTGTTCTTTC CTATATGTAT ATGTATATAT CTATATCTAT GATACAGTCT AATTTATAAA 000720
000721 TTAGGCACAG TGAGAGATTA ACAACAATAA CTAATAGTAA AATAGGACAA TGATGACAAT ATAATGTAAT AAAAGTTTTG 000800
000801 TGAATATGGT CTCTCTTCTC TCTCAAAAAA AAAAAAAAAA AAAAAAAAA
000081 TGTCCCCGGC CTCGCCGTTC CCGTGGCACC CCGGGCACCC CGGCACGGAC GCCACGCCCG CCGCGCTCAC CTCTCCCGCC 000160
000161 GCATGGCGCG GGCGCCGGGG TGGAGTTCTG GCTTCAGGAT CTGCTCGCCG CCGACGACGA CCTCGACCCT TCCATTCAGA 000240
000241 CCCGCGAGGA CGCGCGCCCC GCCCCGGCGC CCGCCCCGTC CACGCCCGAG CCGGGAGCGC CAGGTCCACG GGGCTTCCCG 000320
000321 CGCTCTTGGG GGGCTCCTGC CGCGGCGTCG GGGGCCTGGG CAGCGAGGGC CTCCAGAGGA ACCAGCGCTG CCGACTCCTT 000400
000401 GATTCTAACC CACTGAGGCC TATTTTGGAT TTCTGCACCC CAGAACTGTG GGAGAGTAAA TTGTGCTGTT GAAGCCACCA 000480
000481 CATTTGTGGT GATCTGTTCC AGCTGCAGTA GGAAGCTCGT ATGCTTGGCA GTTCTGATTG TGAACTCACC TTTATGTTGG 000560
000561 AGATTCCTTT ACACATAAGT CCCCTTATCC ACAGCGGACA AATTCCATGA CCCCCAGTGG ATGCCTGAAA TCGCGGAGAG 000640
000641 TACTGAATCC TACATATAAT GTGTTCTTTC CTATATGTAT ATGTATATAT CTATATCTAT GATACAGTCT AATTTATAAA 000720
000721 TTAGGCACAG TGAGAGATTA ACAACAATAA CTAATAGTAA AATAGGACAA TGATGACAAT ATAATGTAAT AAAAGTTTTG 000800
000801 TGAATATGGT CTCTCTTCTC TCTCAAAAAA AAAAAAAAAA AAAAAAAAA
Labs working on this lncRNA
- University Women's Hospital Basel and Research Department of University Hospital, CH-4031 Basel, Switzerland.[1]
