Difference between revisions of "EGFR-AS1"
Chunlei Yu (talk | contribs) (Created page with "==Annotated Information== ===Approved Symbol=== EGFR-AS1 ===Approved Name=== EGFR antisense RNA 1 ===Previous Symbols=== _ ===Synonyms=== _ ===Chromosome=== 7p11.2 ===RefSeq I...") |
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==Annotated Information== | ==Annotated Information== | ||
===Approved Symbol=== | ===Approved Symbol=== | ||
| − | EGFR-AS1 | + | EGFR-AS1 (EGFR antisense RNA 1) |
| − | |||
| − | EGFR antisense RNA 1 | ||
| − | |||
| − | |||
| − | |||
| − | |||
===Chromosome=== | ===Chromosome=== | ||
7p11.2 | 7p11.2 | ||
===RefSeq ID=== | ===RefSeq ID=== | ||
NR_047551 | NR_047551 | ||
| − | |||
| − | |||
===Ensembl ID=== | ===Ensembl ID=== | ||
ENSG00000224057 | ENSG00000224057 | ||
| − | === | + | [[File:Inhibition of EGFR-AS1 impeded cell migration in vitro.png|right|thumb|400px|''' Inhibition of EGFR-AS1 impeded cell migration in vitro'''<ref name="ref1" />]] |
| − | + | ||
| + | ===Characteristic=== | ||
| + | EGFR-AS1 locates on chromosome 7 and can be transcribed as a 2.821-kb lncRNA in the direction opposite to that of EGFR gene transcription.<ref name="ref1" /> | ||
| + | |||
| + | ===Expression=== | ||
| + | {| class='wikitable' style="text-align:center" | ||
| + | |- | ||
| + | ! | Experiment | ||
| + | ! | Forward primer | ||
| + | ! | Reverse primer | ||
| + | |- | ||
| + | | rowspan="1"|Quantitative PCR | ||
| + | | | 5′-CCATCACGTAGGCTTCCTGG-3′ | ||
| + | | | 5′-GCATTCATGCGTCTTCACCTG-3′<ref name="ref1" /> | ||
| + | |} | ||
| + | EGFR-AS1 is upregulated in hepatocellular carcinoma.<ref name="ref1" /> | ||
| + | |||
| + | ===Regulation=== | ||
| + | GHR knockdown can decrease the expression of EGFR-AS1 in vitro.<ref name="ref1" /> | ||
| + | |||
| + | ===Function=== | ||
| + | EGFR-AS1 is upregulated in hepatocellular carcinoma tissues (P=0.0127) and correlated with poor prognosis.<ref name="ref1" /> | ||
| + | EGFR-AS1 expression levels in HCC are significantly associated with portal vein thrombosis (P=0.0261) and lymph metastasis (P=0.0237).<ref name="ref1" /> | ||
| + | EGFR-AS1 knockdown decrease the expression of EGFR and impeded cell proliferation and cell migration in vitro.<ref name="ref1" /> | ||
| + | The overregulation of EGFR-AS1 expression induce increased apoptosis compared with the negative control cells.<ref name="ref1" /> | ||
| + | |||
| + | ==Labs working on this lncRNA== | ||
| + | * Department of General Surgery, Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University, Wuhan, 430071, People's Republic of China. <ref name="ref1" /> | ||
| + | |||
| + | ==References== | ||
| + | <references> | ||
| + | <ref name="ref1"> Qi HL, Li CS, Qian CW, Xiao YS, Yuan YF, Liu QY, Liu ZS. The long noncoding RNA, EGFR-AS1, a target of GHR, increases the expression of EGFR in hepatocellular carcinoma. Tumour Biol. 2016 Jan;37(1):1079-89. doi:10.1007/s13277-015-3887-z. Epub 2015 Aug 14. | ||
| + | </ref>(1) | ||
| + | </references> | ||
| + | |||
===Sequence=== | ===Sequence=== | ||
>gi|383792179|ref|NR_047551.1| Homo sapiens EGFR antisense RNA 1 (EGFR-AS1), long non-coding RNA | >gi|383792179|ref|NR_047551.1| Homo sapiens EGFR antisense RNA 1 (EGFR-AS1), long non-coding RNA | ||
<dnaseq>CCATTTCTACACAGTGTCTGTTTCCTCAGAACACCGGTCTTCACCAAAGGCGTGGGAAAGGGCAGAGAGCACAGGACATATTCTGGAATTCAGGTCACCTTTTACATCTGCCTGGAGTTGGGTGAGCGCCTCACGAATGACTCGGCAGGACTGACTGCCACTGCTAACCAGGCGGATGCAAGCAATGAGGAGGACCTGCCCAGGCCGGGTGGGTGTCTGCTCCCTCCCTCTGGTCCCTGGCACATCTCTGGACCCCCTGCCCTGCTGTCGGAGAGAGATGACGGGCAACGGCGTATTCTCAGAGACAGGGCCTGCCTGCAAATCCTTTAAAGTCAATGGGATGAAATGTATACCCATTGTTAGAAAAAAATAGGACTTAGCAAGTTGAGTGCAAATAACTGATGCAAGACTGGGATGGAGATGGGAGGGGTTTGGGGCAAAAGCAGAAGTCTTTCTGGGTCCGCACCAGCTGTGAAATACCTGGCTTGTTGTTCTGTGCCTGTCTCCAGCACCCAGGCAGGGCTACCTGACCACTCTGCTCTCTCAGCCCCGCCCTGGCTCTGGAGCGAGCCTGTGGAAAGGGGGACACTTAGCCAAGGCCCCAGCCACATAGCAGCAGCAGCTGCGCCCTCTGTCAGCTCCTTGCACCTCCTCACTGGGCCTCCTGCAAGGCACCTGCTCCACTCCACCACTATCACCTGGGTCCTCCTGGCCTTGGCCTGGCTTGCTTACCTTGTTATCAAGTCCTGAATGGGGGAAGCAATATTCCTTCTCCACTACAAATCACCACAGTATTCACAAAGAATTCCAGAGAAATAAGAACAGAGACATCAGACCACACTGAGCACTCAATAAAGAGAAAATTCTTCAAAGGTAGCTGATTGATGAGAGTTTCCACATGCAGATGGGACAGGCACTGATTTGTGCACAAGAAGATCAACTTGATTGAATCCAAAATAAAGGAATGTGTGTGTGCTGCATGCACGCACACACATATCCCCATGGCAAACTCTTGCTATCCCAGGAGCGCAGACCGCATGTGAGGATCCTGGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGATCTGCACACACCAGTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGATGAGCTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAGTGTGGCTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAGACGCATGAATGCGATCTTGAGTTTCAAAATACGTACTCATGGAGGAAAAGCTGTGCCTGCAAAAGACCTAGCACAGGGACGTTTACGCAGGGCTGTGAAGTGACAGATGCAGTGGGAGAGGGCCCCTCCTGGGTGCATCTGGGGATTCCCCATGACAGAGAGGCCCAGGCAACAGTGGCCATGAGGAGCACATTGGATAAAGGAGGAGTCGGAGTCACCTGATCTCTGAGTTTGGGAACTGATAGTATCTTTGTTATGAAGACCTCCGCCTCAAGGTTGAGGATGCTGTGTTTTAAAATATCATGAGGGCCTGTAGGAATCTGTGTGGGGTCCGGAACACCCTGGGTAATGACTGACCCTGCACATCAGGAACGCTGGCTGTGGGTGCTGCAGAGGACAAGCGATGGAGAAGGCATCCGGGGAGCACCGAGCCAGCAGGGAGAAAGGCCTCCCTTCCCCACAGGCCAGGCTTGGCCCTGACTGTGCTCTGGGAAATGGGTGGGCATTTGGGCTGGGGACCCTGCCCACAGCACCTCTGCAAAGAGTAGCTGGATAAGCTCTTTCAATAGACCAGTCCCAGGTTTTGAAATGGACAGAGCATTCAATCTACAGTGACTAAAGGCTGCCTGGCTGCCCGGGACCCATTTCTAAAGAGAAGTGGTCTCTCTGTGCTGTGCCCCCAGGCTCCCTATGGGAAATCCATGCTGCACTGAGTCAGGCATCTGCTGCCCTGCTAATTCCGGCTGGCTGCCAAGGCAGGGGCCTTCCTTTGACAGAGCCATAAATACAGACTTTATTTTAACCCTTCTGCTATTCTTGGGCTGAGGAAGCTAAATTTATTTGCAATCAGGCACACAATGGGGCCCTCTTTTCTGTCTGACTGAGAATGAGGGAATCCCCAATTTCCACCCATAAATTCTCTTTCTCTTTAAAATACAAATGGTGGTGACCTTTTATTCAGATATGGAAAAGAACACACAGACTGTAGCAGAAAGCATCCACAGCTGCTTTTCACATCTCAGCAATGCCTATGTTTTGAGTGTGGACTTGGGCAAGTTAGTTTCTCTGCAGAAGTGAACACACTGAGCCAGGCTCTGAGATAGGGTGCTGCTCCAGGGTGCCCGGGCAGGTCAGGAGCAACAGGCTGGCGGGAGGCAGGGTGGAGATAGGAGACAGGAGACAAAGGCAAGGTGGGGCGAGGGGACACAGACAGTGGACCCTCAGTATCTGGGGAATTGGTTCCAGGACCTCCCTTAAATATCAAAATGTGAGGATGCTCAAGCCCCTGATATAAAGTGGCACAGCATTTGTGTGTAACCTACAGACATCCTCCCATCTACAGCATCTCCTGATTACCTATAGTACCTAATACAGTGCAAATGCTATGTGAATAATTGTTATGCTGTATTGTTTAGGGAATAATGACAAGAAAAAAAGTCCGTATATGTTCAGTACCGATGCAA</dnaseq> | <dnaseq>CCATTTCTACACAGTGTCTGTTTCCTCAGAACACCGGTCTTCACCAAAGGCGTGGGAAAGGGCAGAGAGCACAGGACATATTCTGGAATTCAGGTCACCTTTTACATCTGCCTGGAGTTGGGTGAGCGCCTCACGAATGACTCGGCAGGACTGACTGCCACTGCTAACCAGGCGGATGCAAGCAATGAGGAGGACCTGCCCAGGCCGGGTGGGTGTCTGCTCCCTCCCTCTGGTCCCTGGCACATCTCTGGACCCCCTGCCCTGCTGTCGGAGAGAGATGACGGGCAACGGCGTATTCTCAGAGACAGGGCCTGCCTGCAAATCCTTTAAAGTCAATGGGATGAAATGTATACCCATTGTTAGAAAAAAATAGGACTTAGCAAGTTGAGTGCAAATAACTGATGCAAGACTGGGATGGAGATGGGAGGGGTTTGGGGCAAAAGCAGAAGTCTTTCTGGGTCCGCACCAGCTGTGAAATACCTGGCTTGTTGTTCTGTGCCTGTCTCCAGCACCCAGGCAGGGCTACCTGACCACTCTGCTCTCTCAGCCCCGCCCTGGCTCTGGAGCGAGCCTGTGGAAAGGGGGACACTTAGCCAAGGCCCCAGCCACATAGCAGCAGCAGCTGCGCCCTCTGTCAGCTCCTTGCACCTCCTCACTGGGCCTCCTGCAAGGCACCTGCTCCACTCCACCACTATCACCTGGGTCCTCCTGGCCTTGGCCTGGCTTGCTTACCTTGTTATCAAGTCCTGAATGGGGGAAGCAATATTCCTTCTCCACTACAAATCACCACAGTATTCACAAAGAATTCCAGAGAAATAAGAACAGAGACATCAGACCACACTGAGCACTCAATAAAGAGAAAATTCTTCAAAGGTAGCTGATTGATGAGAGTTTCCACATGCAGATGGGACAGGCACTGATTTGTGCACAAGAAGATCAACTTGATTGAATCCAAAATAAAGGAATGTGTGTGTGCTGCATGCACGCACACACATATCCCCATGGCAAACTCTTGCTATCCCAGGAGCGCAGACCGCATGTGAGGATCCTGGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGATCTGCACACACCAGTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGATGAGCTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAGTGTGGCTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAGACGCATGAATGCGATCTTGAGTTTCAAAATACGTACTCATGGAGGAAAAGCTGTGCCTGCAAAAGACCTAGCACAGGGACGTTTACGCAGGGCTGTGAAGTGACAGATGCAGTGGGAGAGGGCCCCTCCTGGGTGCATCTGGGGATTCCCCATGACAGAGAGGCCCAGGCAACAGTGGCCATGAGGAGCACATTGGATAAAGGAGGAGTCGGAGTCACCTGATCTCTGAGTTTGGGAACTGATAGTATCTTTGTTATGAAGACCTCCGCCTCAAGGTTGAGGATGCTGTGTTTTAAAATATCATGAGGGCCTGTAGGAATCTGTGTGGGGTCCGGAACACCCTGGGTAATGACTGACCCTGCACATCAGGAACGCTGGCTGTGGGTGCTGCAGAGGACAAGCGATGGAGAAGGCATCCGGGGAGCACCGAGCCAGCAGGGAGAAAGGCCTCCCTTCCCCACAGGCCAGGCTTGGCCCTGACTGTGCTCTGGGAAATGGGTGGGCATTTGGGCTGGGGACCCTGCCCACAGCACCTCTGCAAAGAGTAGCTGGATAAGCTCTTTCAATAGACCAGTCCCAGGTTTTGAAATGGACAGAGCATTCAATCTACAGTGACTAAAGGCTGCCTGGCTGCCCGGGACCCATTTCTAAAGAGAAGTGGTCTCTCTGTGCTGTGCCCCCAGGCTCCCTATGGGAAATCCATGCTGCACTGAGTCAGGCATCTGCTGCCCTGCTAATTCCGGCTGGCTGCCAAGGCAGGGGCCTTCCTTTGACAGAGCCATAAATACAGACTTTATTTTAACCCTTCTGCTATTCTTGGGCTGAGGAAGCTAAATTTATTTGCAATCAGGCACACAATGGGGCCCTCTTTTCTGTCTGACTGAGAATGAGGGAATCCCCAATTTCCACCCATAAATTCTCTTTCTCTTTAAAATACAAATGGTGGTGACCTTTTATTCAGATATGGAAAAGAACACACAGACTGTAGCAGAAAGCATCCACAGCTGCTTTTCACATCTCAGCAATGCCTATGTTTTGAGTGTGGACTTGGGCAAGTTAGTTTCTCTGCAGAAGTGAACACACTGAGCCAGGCTCTGAGATAGGGTGCTGCTCCAGGGTGCCCGGGCAGGTCAGGAGCAACAGGCTGGCGGGAGGCAGGGTGGAGATAGGAGACAGGAGACAAAGGCAAGGTGGGGCGAGGGGACACAGACAGTGGACCCTCAGTATCTGGGGAATTGGTTCCAGGACCTCCCTTAAATATCAAAATGTGAGGATGCTCAAGCCCCTGATATAAAGTGGCACAGCATTTGTGTGTAACCTACAGACATCCTCCCATCTACAGCATCTCCTGATTACCTATAGTACCTAATACAGTGCAAATGCTATGTGAATAATTGTTATGCTGTATTGTTTAGGGAATAATGACAAGAAAAAAAGTCCGTATATGTTCAGTACCGATGCAA</dnaseq> | ||
Latest revision as of 14:56, 26 January 2018
Contents
Annotated Information
Approved Symbol
EGFR-AS1 (EGFR antisense RNA 1)
Chromosome
7p11.2
RefSeq ID
NR_047551
Ensembl ID
ENSG00000224057
Inhibition of EGFR-AS1 impeded cell migration in vitro[1]
Characteristic
EGFR-AS1 locates on chromosome 7 and can be transcribed as a 2.821-kb lncRNA in the direction opposite to that of EGFR gene transcription.[1]
Expression
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| Quantitative PCR | 5′-CCATCACGTAGGCTTCCTGG-3′ | 5′-GCATTCATGCGTCTTCACCTG-3′[1] |
EGFR-AS1 is upregulated in hepatocellular carcinoma.[1]
Regulation
GHR knockdown can decrease the expression of EGFR-AS1 in vitro.[1]
Function
EGFR-AS1 is upregulated in hepatocellular carcinoma tissues (P=0.0127) and correlated with poor prognosis.[1] EGFR-AS1 expression levels in HCC are significantly associated with portal vein thrombosis (P=0.0261) and lymph metastasis (P=0.0237).[1] EGFR-AS1 knockdown decrease the expression of EGFR and impeded cell proliferation and cell migration in vitro.[1] The overregulation of EGFR-AS1 expression induce increased apoptosis compared with the negative control cells.[1]
Labs working on this lncRNA
- Department of General Surgery, Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University, Wuhan, 430071, People's Republic of China. [1]
References
Sequence
>gi|383792179|ref|NR_047551.1| Homo sapiens EGFR antisense RNA 1 (EGFR-AS1), long non-coding RNA
000001 CCATTTCTAC ACAGTGTCTG TTTCCTCAGA ACACCGGTCT TCACCAAAGG CGTGGGAAAG GGCAGAGAGC ACAGGACATA 000080
000081 TTCTGGAATT CAGGTCACCT TTTACATCTG CCTGGAGTTG GGTGAGCGCC TCACGAATGA CTCGGCAGGA CTGACTGCCA 000160
000161 CTGCTAACCA GGCGGATGCA AGCAATGAGG AGGACCTGCC CAGGCCGGGT GGGTGTCTGC TCCCTCCCTC TGGTCCCTGG 000240
000241 CACATCTCTG GACCCCCTGC CCTGCTGTCG GAGAGAGATG ACGGGCAACG GCGTATTCTC AGAGACAGGG CCTGCCTGCA 000320
000321 AATCCTTTAA AGTCAATGGG ATGAAATGTA TACCCATTGT TAGAAAAAAA TAGGACTTAG CAAGTTGAGT GCAAATAACT 000400
000401 GATGCAAGAC TGGGATGGAG ATGGGAGGGG TTTGGGGCAA AAGCAGAAGT CTTTCTGGGT CCGCACCAGC TGTGAAATAC 000480
000481 CTGGCTTGTT GTTCTGTGCC TGTCTCCAGC ACCCAGGCAG GGCTACCTGA CCACTCTGCT CTCTCAGCCC CGCCCTGGCT 000560
000561 CTGGAGCGAG CCTGTGGAAA GGGGGACACT TAGCCAAGGC CCCAGCCACA TAGCAGCAGC AGCTGCGCCC TCTGTCAGCT 000640
000641 CCTTGCACCT CCTCACTGGG CCTCCTGCAA GGCACCTGCT CCACTCCACC ACTATCACCT GGGTCCTCCT GGCCTTGGCC 000720
000721 TGGCTTGCTT ACCTTGTTAT CAAGTCCTGA ATGGGGGAAG CAATATTCCT TCTCCACTAC AAATCACCAC AGTATTCACA 000800
000801 AAGAATTCCA GAGAAATAAG AACAGAGACA TCAGACCACA CTGAGCACTC AATAAAGAGA AAATTCTTCA AAGGTAGCTG 000880
000881 ATTGATGAGA GTTTCCACAT GCAGATGGGA CAGGCACTGA TTTGTGCACA AGAAGATCAA CTTGATTGAA TCCAAAATAA 000960
000961 AGGAATGTGT GTGTGCTGCA TGCACGCACA CACATATCCC CATGGCAAAC TCTTGCTATC CCAGGAGCGC AGACCGCATG 001040
001041 TGAGGATCCT GGCTCCTTAT CTCCCCTCCC CGTATCTCCC TTCCCTGATT ACCTTTGCGA TCTGCACACA CCAGTTGAGC 001120
001121 AGGTACTGGG AGCCAATATT GTCTTTGTGT TCCCGGACAT AGTCCAGGAG GCAGCCGAAG GGCATGAGCT GCGTGATGAG 001200
001201 CTGCACGGTG GAGGTGAGGC AGATGCCCAG CAGGCGGCAC ACGTGGGGGT TGTCCACGCT GGCCATCACG TAGGCTTCCT 001280
001281 GGAGGGAGGG AGAGGCACGT CAGTGTGGCT TCGCATGGTG GCCAGAAGGA GGGGCACATG GACCCCTTCC AGGTGAAGAC 001360
001361 GCATGAATGC GATCTTGAGT TTCAAAATAC GTACTCATGG AGGAAAAGCT GTGCCTGCAA AAGACCTAGC ACAGGGACGT 001440
001441 TTACGCAGGG CTGTGAAGTG ACAGATGCAG TGGGAGAGGG CCCCTCCTGG GTGCATCTGG GGATTCCCCA TGACAGAGAG 001520
001521 GCCCAGGCAA CAGTGGCCAT GAGGAGCACA TTGGATAAAG GAGGAGTCGG AGTCACCTGA TCTCTGAGTT TGGGAACTGA 001600
001601 TAGTATCTTT GTTATGAAGA CCTCCGCCTC AAGGTTGAGG ATGCTGTGTT TTAAAATATC ATGAGGGCCT GTAGGAATCT 001680
001681 GTGTGGGGTC CGGAACACCC TGGGTAATGA CTGACCCTGC ACATCAGGAA CGCTGGCTGT GGGTGCTGCA GAGGACAAGC 001760
001761 GATGGAGAAG GCATCCGGGG AGCACCGAGC CAGCAGGGAG AAAGGCCTCC CTTCCCCACA GGCCAGGCTT GGCCCTGACT 001840
001841 GTGCTCTGGG AAATGGGTGG GCATTTGGGC TGGGGACCCT GCCCACAGCA CCTCTGCAAA GAGTAGCTGG ATAAGCTCTT 001920
001921 TCAATAGACC AGTCCCAGGT TTTGAAATGG ACAGAGCATT CAATCTACAG TGACTAAAGG CTGCCTGGCT GCCCGGGACC 002000
002001 CATTTCTAAA GAGAAGTGGT CTCTCTGTGC TGTGCCCCCA GGCTCCCTAT GGGAAATCCA TGCTGCACTG AGTCAGGCAT 002080
002081 CTGCTGCCCT GCTAATTCCG GCTGGCTGCC AAGGCAGGGG CCTTCCTTTG ACAGAGCCAT AAATACAGAC TTTATTTTAA 002160
002161 CCCTTCTGCT ATTCTTGGGC TGAGGAAGCT AAATTTATTT GCAATCAGGC ACACAATGGG GCCCTCTTTT CTGTCTGACT 002240
002241 GAGAATGAGG GAATCCCCAA TTTCCACCCA TAAATTCTCT TTCTCTTTAA AATACAAATG GTGGTGACCT TTTATTCAGA 002320
002321 TATGGAAAAG AACACACAGA CTGTAGCAGA AAGCATCCAC AGCTGCTTTT CACATCTCAG CAATGCCTAT GTTTTGAGTG 002400
002401 TGGACTTGGG CAAGTTAGTT TCTCTGCAGA AGTGAACACA CTGAGCCAGG CTCTGAGATA GGGTGCTGCT CCAGGGTGCC 002480
002481 CGGGCAGGTC AGGAGCAACA GGCTGGCGGG AGGCAGGGTG GAGATAGGAG ACAGGAGACA AAGGCAAGGT GGGGCGAGGG 002560
002561 GACACAGACA GTGGACCCTC AGTATCTGGG GAATTGGTTC CAGGACCTCC CTTAAATATC AAAATGTGAG GATGCTCAAG 002640
002641 CCCCTGATAT AAAGTGGCAC AGCATTTGTG TGTAACCTAC AGACATCCTC CCATCTACAG CATCTCCTGA TTACCTATAG 002720
002721 TACCTAATAC AGTGCAAATG CTATGTGAAT AATTGTTATG CTGTATTGTT TAGGGAATAA TGACAAGAAA AAAAGTCCGT 002800
002801 ATATGTTCAG TACCGATGCA A
000081 TTCTGGAATT CAGGTCACCT TTTACATCTG CCTGGAGTTG GGTGAGCGCC TCACGAATGA CTCGGCAGGA CTGACTGCCA 000160
000161 CTGCTAACCA GGCGGATGCA AGCAATGAGG AGGACCTGCC CAGGCCGGGT GGGTGTCTGC TCCCTCCCTC TGGTCCCTGG 000240
000241 CACATCTCTG GACCCCCTGC CCTGCTGTCG GAGAGAGATG ACGGGCAACG GCGTATTCTC AGAGACAGGG CCTGCCTGCA 000320
000321 AATCCTTTAA AGTCAATGGG ATGAAATGTA TACCCATTGT TAGAAAAAAA TAGGACTTAG CAAGTTGAGT GCAAATAACT 000400
000401 GATGCAAGAC TGGGATGGAG ATGGGAGGGG TTTGGGGCAA AAGCAGAAGT CTTTCTGGGT CCGCACCAGC TGTGAAATAC 000480
000481 CTGGCTTGTT GTTCTGTGCC TGTCTCCAGC ACCCAGGCAG GGCTACCTGA CCACTCTGCT CTCTCAGCCC CGCCCTGGCT 000560
000561 CTGGAGCGAG CCTGTGGAAA GGGGGACACT TAGCCAAGGC CCCAGCCACA TAGCAGCAGC AGCTGCGCCC TCTGTCAGCT 000640
000641 CCTTGCACCT CCTCACTGGG CCTCCTGCAA GGCACCTGCT CCACTCCACC ACTATCACCT GGGTCCTCCT GGCCTTGGCC 000720
000721 TGGCTTGCTT ACCTTGTTAT CAAGTCCTGA ATGGGGGAAG CAATATTCCT TCTCCACTAC AAATCACCAC AGTATTCACA 000800
000801 AAGAATTCCA GAGAAATAAG AACAGAGACA TCAGACCACA CTGAGCACTC AATAAAGAGA AAATTCTTCA AAGGTAGCTG 000880
000881 ATTGATGAGA GTTTCCACAT GCAGATGGGA CAGGCACTGA TTTGTGCACA AGAAGATCAA CTTGATTGAA TCCAAAATAA 000960
000961 AGGAATGTGT GTGTGCTGCA TGCACGCACA CACATATCCC CATGGCAAAC TCTTGCTATC CCAGGAGCGC AGACCGCATG 001040
001041 TGAGGATCCT GGCTCCTTAT CTCCCCTCCC CGTATCTCCC TTCCCTGATT ACCTTTGCGA TCTGCACACA CCAGTTGAGC 001120
001121 AGGTACTGGG AGCCAATATT GTCTTTGTGT TCCCGGACAT AGTCCAGGAG GCAGCCGAAG GGCATGAGCT GCGTGATGAG 001200
001201 CTGCACGGTG GAGGTGAGGC AGATGCCCAG CAGGCGGCAC ACGTGGGGGT TGTCCACGCT GGCCATCACG TAGGCTTCCT 001280
001281 GGAGGGAGGG AGAGGCACGT CAGTGTGGCT TCGCATGGTG GCCAGAAGGA GGGGCACATG GACCCCTTCC AGGTGAAGAC 001360
001361 GCATGAATGC GATCTTGAGT TTCAAAATAC GTACTCATGG AGGAAAAGCT GTGCCTGCAA AAGACCTAGC ACAGGGACGT 001440
001441 TTACGCAGGG CTGTGAAGTG ACAGATGCAG TGGGAGAGGG CCCCTCCTGG GTGCATCTGG GGATTCCCCA TGACAGAGAG 001520
001521 GCCCAGGCAA CAGTGGCCAT GAGGAGCACA TTGGATAAAG GAGGAGTCGG AGTCACCTGA TCTCTGAGTT TGGGAACTGA 001600
001601 TAGTATCTTT GTTATGAAGA CCTCCGCCTC AAGGTTGAGG ATGCTGTGTT TTAAAATATC ATGAGGGCCT GTAGGAATCT 001680
001681 GTGTGGGGTC CGGAACACCC TGGGTAATGA CTGACCCTGC ACATCAGGAA CGCTGGCTGT GGGTGCTGCA GAGGACAAGC 001760
001761 GATGGAGAAG GCATCCGGGG AGCACCGAGC CAGCAGGGAG AAAGGCCTCC CTTCCCCACA GGCCAGGCTT GGCCCTGACT 001840
001841 GTGCTCTGGG AAATGGGTGG GCATTTGGGC TGGGGACCCT GCCCACAGCA CCTCTGCAAA GAGTAGCTGG ATAAGCTCTT 001920
001921 TCAATAGACC AGTCCCAGGT TTTGAAATGG ACAGAGCATT CAATCTACAG TGACTAAAGG CTGCCTGGCT GCCCGGGACC 002000
002001 CATTTCTAAA GAGAAGTGGT CTCTCTGTGC TGTGCCCCCA GGCTCCCTAT GGGAAATCCA TGCTGCACTG AGTCAGGCAT 002080
002081 CTGCTGCCCT GCTAATTCCG GCTGGCTGCC AAGGCAGGGG CCTTCCTTTG ACAGAGCCAT AAATACAGAC TTTATTTTAA 002160
002161 CCCTTCTGCT ATTCTTGGGC TGAGGAAGCT AAATTTATTT GCAATCAGGC ACACAATGGG GCCCTCTTTT CTGTCTGACT 002240
002241 GAGAATGAGG GAATCCCCAA TTTCCACCCA TAAATTCTCT TTCTCTTTAA AATACAAATG GTGGTGACCT TTTATTCAGA 002320
002321 TATGGAAAAG AACACACAGA CTGTAGCAGA AAGCATCCAC AGCTGCTTTT CACATCTCAG CAATGCCTAT GTTTTGAGTG 002400
002401 TGGACTTGGG CAAGTTAGTT TCTCTGCAGA AGTGAACACA CTGAGCCAGG CTCTGAGATA GGGTGCTGCT CCAGGGTGCC 002480
002481 CGGGCAGGTC AGGAGCAACA GGCTGGCGGG AGGCAGGGTG GAGATAGGAG ACAGGAGACA AAGGCAAGGT GGGGCGAGGG 002560
002561 GACACAGACA GTGGACCCTC AGTATCTGGG GAATTGGTTC CAGGACCTCC CTTAAATATC AAAATGTGAG GATGCTCAAG 002640
002641 CCCCTGATAT AAAGTGGCAC AGCATTTGTG TGTAACCTAC AGACATCCTC CCATCTACAG CATCTCCTGA TTACCTATAG 002720
002721 TACCTAATAC AGTGCAAATG CTATGTGAAT AATTGTTATG CTGTATTGTT TAGGGAATAA TGACAAGAAA AAAAGTCCGT 002800
002801 ATATGTTCAG TACCGATGCA A
