Difference between revisions of "BTG3-AS1"
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| + | ''BTB3-AS1'', an antinsense transcript required for tumorogenicity of ovarian and colorectal cancer cells | ||
| + | |||
==Annotated Information== | ==Annotated Information== | ||
===Name=== | ===Name=== | ||
| − | + | ''BTB3-AS1'': BTG3 antisense RNA 1 | |
| − | + | ASBEL <ref name="ref1" /> | |
| − | ASBEL<ref name="ref1" /> | ||
===Characteristics=== | ===Characteristics=== | ||
| + | ''BTB3-AS1'' is a member of the TOB/BTG family of antiproliferative genes that regulates cell cycle progression in a variety of cell types <ref name="ref3" /> | ||
[[File:ASBEL-1.JPG|right|thumb|400px|'''ASBEL is transcribed from the DNA strand opposite to ANA/BTG3'''<ref name="ref1" />.]] | [[File:ASBEL-1.JPG|right|thumb|400px|'''ASBEL is transcribed from the DNA strand opposite to ANA/BTG3'''<ref name="ref1" />.]] | ||
| − | + | ''BTB3-AS1'' is an antisense transcript of ANA/BTG3. it is a highly conserved gene encoded by the DNA strand opposite the ANA/BTG3 gene. | |
===Function=== | ===Function=== | ||
| − | In ovarian carcinoma, | + | In ovarian carcinoma, ''BTB3-AS1'' promotes tumorigenesis through suppressing translation of the sense gene (ANA/BTG3) <ref name="ref1" />. ''BTB3-AS1'' forms duplexes with ANA/BTG3 mRNA in the nucleus and suppresses its cytoplasmic transportation, leading to negative regulation of ANA/BTG3 at protein level without changing of its mRNA level <ref name="ref1" />. |
| − | + | ''BTB3-AS1'' also plays a key role in Wnt/β-catenin-mediated tumorigenesis <ref name="ref2" />. ''BTB3-AS1'' interacts with and recruits transcription factor 3 (TCF3) for activating transcription factor 3 (ATF3) locus and represses the expression of ATF3, resulting in proliferation and tumorigenicity of colon tumor cells <ref name="ref2" />. | |
| − | |||
===Regulation=== | ===Regulation=== | ||
| − | β-catenin is found to directly enhance the transcription of | + | β-catenin is found to directly enhance the transcription of ''BTB3-AS1'' <ref name="ref2" />. |
| − | |||
===Disease=== | ===Disease=== | ||
| − | + | *Colorectal cancer <ref name="ref2" /> | |
| + | *Ovarian carcinoma <ref name="ref1" /> | ||
| − | |||
===Expression=== | ===Expression=== | ||
| − | + | ''BTB3-AS1'' is highly expressed in stage I–III colon cancer than in normal tissues <ref name="ref2" />. | |
==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | *Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. | + | *Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. |
| − | *Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan. | + | *Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan. |
| + | *Division of Oncology, Department of Cancer Biology, Institute of Medical Science, The University of Tokyo, 461 Shirokanedai, Tokyo, 108-8639, Japan. | ||
| + | |||
| + | |||
==References== | ==References== | ||
<references> | <references> | ||
| − | <ref name="ref1"> | + | <ref name="ref1">Yanagida S, Taniue K, Sugimasa H, Nasu E, Takeda Y, Kobayashi M, Yamamoto T,Okamoto A, Akiyama T. ASBEL, an ANA/BTG3 antisense transcript required fortumorigenicity of ovarian carcinoma. Sci Rep. 2013;3:1305. |
| − | Yanagida S, Taniue K, Sugimasa H, Nasu E, Takeda Y, Kobayashi M, Yamamoto T, | + | </ref>(1) |
| − | Okamoto A, Akiyama T. ASBEL, an ANA/BTG3 antisense transcript required | + | <ref name="ref2">Taniue K, Kurimoto A, Takeda Y, Nagashima T, Okada-Hatakeyama M, Katou Y, Shirahige K, Akiyama T. ASBEL-TCF3 complex is required for the tumorigenicity of colorectal cancer cells. Proc Natl Acad Sci U S A. 2016 Oct 21. |
| − | + | </ref>(2) | |
| − | </ref> | + | <ref name="ref3">Winkler, G. S. The mammalian anti-proliferative BTG/Tob protein family. J. Cell. |
| − | <ref name="ref2"> | + | Phys. 222, 66–72 (2010). |
| − | Taniue K, Kurimoto A, Takeda Y, Nagashima T, Okada-Hatakeyama M, Katou Y, | + | </ref>(3) |
| − | Shirahige K, Akiyama T. ASBEL-TCF3 complex is required for the tumorigenicity of | ||
| − | colorectal cancer cells. Proc Natl Acad Sci U S A. 2016 Oct 21. | ||
</references> | </references> | ||
| − | + | ===Sequence=== | |
| + | >gi|110806272|ref|NR_149073.1|Homo sapiens BTG3 antisense RNA 1 (BTG3-AS1), long non-coding RNA | ||
| + | <dnaseq>ATCGCACGGTGACGTCATCCTGGGACCTCGTCACCGCGGCGGCTGCTCCTCAAGTTCTACTTTGTTCCAC | ||
| + | AAGGCTCCTCTGCTCACGGCTGCGTGGATAAAACCCAAAGGGAATCCAGAATCAAGCTCGGCCTCGCAGG | ||
| + | AGTTTATAACTTTTGTACTTTTCCCTCCCTTGTAACGAAAAGCTAATCGCAAACGTTTCTAGGAACACAT | ||
| + | TTTTCTCCTGATAGACGTTCCCAAGGTGGAGGGCGCGAGAAGACTGGCCCGTGGGGTGAGGAGCCCCGGC | ||
| + | CCGACCTCAGCGGCGCCACAAAGCCATGAAGAGGGGAAATAATTTTCCAAGGGGCTAAGAGTTGGACTTT | ||
| + | CTGAAGCCATCGAAACCTGCTGCTAGGCTCTCCCGCTGGGGTTTTGAGAGAGGTAGGTGAGGAGGGATCA | ||
| + | AACATCTCCACCCGCTCTTGAAAGAACCGCCTTGAGCTGCGCCCAACTTCTGCAATCCCGGTTTTCCCCA | ||
| + | AGTTCAAGGGGAAGGGCCCTGGCGCCCAATGCCCAGCCTCCCCGACAACATCCTCGCCAGGGTGCGCCTG | ||
| + | CCCCGCGGGTCCGCTGTCCCCGGCGGGCGGCCCTGGGTTCGAGGACCCGGCTTCCCGGAGGCGCGACTCT | ||
| + | CCAGCAAACAAGCGGAGCTCCAGAAAAAACACCGACGCGAAGGGGGTGGACAGAGGGTGGGAAGCCGCGG | ||
| + | GGTTGTCGCCCACCCGGAGGCGGAATGTAACGCGCTGTGGGAAACGTGGCAGGAGCCCTGAGGCGGCGTC | ||
| + | TTTTTCCTCAGGCGCCCGCCGAGGGTCCCGCGGAAAGCCTCGCGGGCCGCCCTCGCGGCCCGCAGCCCCG | ||
| + | CTCTGGCGCCGAGCCGGAGCCCATGCAACCTGGTTCCATCCCCTGCCCCTCCCCTGTCCCCGGTGCGCCG | ||
| + | CCCGCCAGCGAGCCTTCGACGTGGCCGCAGGGGCGACGGGACCACCCTCCCCCGATACCCACAGCCCCGC | ||
| + | CATGTCTGCCTTTCCCCGGCCCGGTCTCCTCACCGCCGCTGCCGCCGCCGCTCTTCGGCCGGAGATTCGG | ||
| + | CGGCCCAGACCGTGTCCTGGCCGGGAACTGAGGGCTCCGCCTCAACGGGCCCGCGCTGGGCAACAGGGAG | ||
| + | CGCAGCGAGCCTCGTCCGGCGCGTGCGGCTCCCGCGTCGTCGGGCGGCCAAGCGCGCGTTGAGAGGACTG | ||
| + | GCGGGCGGACGAGCGCGCACACGAGTGAGCGCAGCCCCAAAGCGGCGCGCAGGGGGCTCGCGGCCCGGAA | ||
| + | GAGGGGAGGGGCGATGACCCGGGAAAGGGTTGGCGCGCGCGGGATCGGCTCGCGCGCCTGAGGGGCGGTG | ||
| + | CCGGGGGCGGGGCTTCGCCGCGAGGCCACCCCCGAGCCCCGGGCCTAGCCGCACGGGAGGCGACACACCC | ||
| + | TCGCCCTACCCTGAGCCTGGCGCAGACCCTCGACCGCGACGGCGCGGTCCGCAGAACCGCGGGCTTCCCT | ||
| + | CCCCGGCAAAAAGCGGCGCTGAAGGCCTGTGGGACCCCCGCCCGGCCGCCCTACGTGCGTCAGCGCCTTC | ||
| + | GGGGGTCGCGCGGGCTTGGTCCTTTCGGCTGTCTCGGCCTTTTTGTTTCCCCCTCAGGTCTCTCCACGCT | ||
| + | GCACTTGACACCCTCAGGGCGGAATGGCGAGTTCTAGACCCAGCTCTCTAGACCCGGGGCTTCATGGGGG | ||
| + | ACACGGACTCGACGGGAAGGGGAACTTGGCATTTATGGACAGATCCTCATCCTTCTTGCTGTAGTCAATT | ||
| + | ACACGCACGTTAACCGTGCAGCCGCCCTGCTGTATTTTAGGCGGTTGTTGGCACTCCTAGTTGGGCCCTT | ||
| + | CCCTGGCCCCTCACAGCAGGGCCTGCCTCCTGTGGACGCTTGTGTGCTGCCCTGGCACCGGCCACTGTGT | ||
| + | TTTGCATAAAGGAGAGGCTCCAAAGATGTTGGCCAAAGGAATGAAGCCTTGAGAGTCCAGGCTTTCTATT | ||
| + | TCTGAGACACTCTACTCAAGGACTTCCCAGATGCAAAGCTTCATCTTTGAGCAAATACAAATATGGAGAG | ||
| + | GGAACATTAACTTTCTGAAGAAAAGAAGGAACATCTTTTCAACCTTTTATATTGAGTTAACACCATGGTC | ||
| + | TTAGTTTTGTGAAAGCACTTTAATACATCCAACTAGCGGGAGGAATACAAGATATTCCTACCTTTTTATT | ||
| + | ATTATTATTAAAAGAGGGCTAAACCATGTTGTAAACTACTTAAGAACTGAATGTTCAAGTTGTACAGTAT | ||
| + | ATTGGCCAGATTCCTTACTGCCCACTAGGCTTGGGAGCATGTTTTCAGCTCAGTTTTATGAATGTTTTAA | ||
| + | ATTTGTATTACTGATCTTTGTTATAGCCAATTAGCAATTCAAAATGGTAATTTTTTTAGCAGTAATAAAT | ||
| + | ATACCATCCCCCAAACAGTACAATAAACTTTATTTGTAAAAAAAAAAAAAAAAAA</dnaseq> | ||
Latest revision as of 05:04, 14 November 2018
BTB3-AS1, an antinsense transcript required for tumorogenicity of ovarian and colorectal cancer cells
Contents
Annotated Information
Name
BTB3-AS1: BTG3 antisense RNA 1 ASBEL [1]
Characteristics
BTB3-AS1 is a member of the TOB/BTG family of antiproliferative genes that regulates cell cycle progression in a variety of cell types [2]
ASBEL is transcribed from the DNA strand opposite to ANA/BTG3[1].
BTB3-AS1 is an antisense transcript of ANA/BTG3. it is a highly conserved gene encoded by the DNA strand opposite the ANA/BTG3 gene.
Function
In ovarian carcinoma, BTB3-AS1 promotes tumorigenesis through suppressing translation of the sense gene (ANA/BTG3) [1]. BTB3-AS1 forms duplexes with ANA/BTG3 mRNA in the nucleus and suppresses its cytoplasmic transportation, leading to negative regulation of ANA/BTG3 at protein level without changing of its mRNA level [1]. BTB3-AS1 also plays a key role in Wnt/β-catenin-mediated tumorigenesis [3]. BTB3-AS1 interacts with and recruits transcription factor 3 (TCF3) for activating transcription factor 3 (ATF3) locus and represses the expression of ATF3, resulting in proliferation and tumorigenicity of colon tumor cells [3].
Regulation
β-catenin is found to directly enhance the transcription of BTB3-AS1 [3].
Disease
Expression
BTB3-AS1 is highly expressed in stage I–III colon cancer than in normal tissues [3].
Labs working on this lncRNA
- Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
- Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
- Division of Oncology, Department of Cancer Biology, Institute of Medical Science, The University of Tokyo, 461 Shirokanedai, Tokyo, 108-8639, Japan.
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Yanagida S, Taniue K, Sugimasa H, Nasu E, Takeda Y, Kobayashi M, Yamamoto T,Okamoto A, Akiyama T. ASBEL, an ANA/BTG3 antisense transcript required fortumorigenicity of ovarian carcinoma. Sci Rep. 2013;3:1305.
- ↑ Winkler, G. S. The mammalian anti-proliferative BTG/Tob protein family. J. Cell. Phys. 222, 66–72 (2010).
- ↑ 3.0 3.1 3.2 3.3 3.4 Taniue K, Kurimoto A, Takeda Y, Nagashima T, Okada-Hatakeyama M, Katou Y, Shirahige K, Akiyama T. ASBEL-TCF3 complex is required for the tumorigenicity of colorectal cancer cells. Proc Natl Acad Sci U S A. 2016 Oct 21.
Sequence
>gi|110806272|ref|NR_149073.1|Homo sapiens BTG3 antisense RNA 1 (BTG3-AS1), long non-coding RNA
000001 ATCGCACGGT GACGTCATCC TGGGACCTCG TCACCGCGGC GGCTGCTCCT CAAGTTCTAC TTTGTTCCAC AAGGCTCCTC 000080
000081 TGCTCACGGC TGCGTGGATA AAACCCAAAG GGAATCCAGA ATCAAGCTCG GCCTCGCAGG AGTTTATAAC TTTTGTACTT 000160
000161 TTCCCTCCCT TGTAACGAAA AGCTAATCGC AAACGTTTCT AGGAACACAT TTTTCTCCTG ATAGACGTTC CCAAGGTGGA 000240
000241 GGGCGCGAGA AGACTGGCCC GTGGGGTGAG GAGCCCCGGC CCGACCTCAG CGGCGCCACA AAGCCATGAA GAGGGGAAAT 000320
000321 AATTTTCCAA GGGGCTAAGA GTTGGACTTT CTGAAGCCAT CGAAACCTGC TGCTAGGCTC TCCCGCTGGG GTTTTGAGAG 000400
000401 AGGTAGGTGA GGAGGGATCA AACATCTCCA CCCGCTCTTG AAAGAACCGC CTTGAGCTGC GCCCAACTTC TGCAATCCCG 000480
000481 GTTTTCCCCA AGTTCAAGGG GAAGGGCCCT GGCGCCCAAT GCCCAGCCTC CCCGACAACA TCCTCGCCAG GGTGCGCCTG 000560
000561 CCCCGCGGGT CCGCTGTCCC CGGCGGGCGG CCCTGGGTTC GAGGACCCGG CTTCCCGGAG GCGCGACTCT CCAGCAAACA 000640
000641 AGCGGAGCTC CAGAAAAAAC ACCGACGCGA AGGGGGTGGA CAGAGGGTGG GAAGCCGCGG GGTTGTCGCC CACCCGGAGG 000720
000721 CGGAATGTAA CGCGCTGTGG GAAACGTGGC AGGAGCCCTG AGGCGGCGTC TTTTTCCTCA GGCGCCCGCC GAGGGTCCCG 000800
000801 CGGAAAGCCT CGCGGGCCGC CCTCGCGGCC CGCAGCCCCG CTCTGGCGCC GAGCCGGAGC CCATGCAACC TGGTTCCATC 000880
000881 CCCTGCCCCT CCCCTGTCCC CGGTGCGCCG CCCGCCAGCG AGCCTTCGAC GTGGCCGCAG GGGCGACGGG ACCACCCTCC 000960
000961 CCCGATACCC ACAGCCCCGC CATGTCTGCC TTTCCCCGGC CCGGTCTCCT CACCGCCGCT GCCGCCGCCG CTCTTCGGCC 001040
001041 GGAGATTCGG CGGCCCAGAC CGTGTCCTGG CCGGGAACTG AGGGCTCCGC CTCAACGGGC CCGCGCTGGG CAACAGGGAG 001120
001121 CGCAGCGAGC CTCGTCCGGC GCGTGCGGCT CCCGCGTCGT CGGGCGGCCA AGCGCGCGTT GAGAGGACTG GCGGGCGGAC 001200
001201 GAGCGCGCAC ACGAGTGAGC GCAGCCCCAA AGCGGCGCGC AGGGGGCTCG CGGCCCGGAA GAGGGGAGGG GCGATGACCC 001280
001281 GGGAAAGGGT TGGCGCGCGC GGGATCGGCT CGCGCGCCTG AGGGGCGGTG CCGGGGGCGG GGCTTCGCCG CGAGGCCACC 001360
001361 CCCGAGC
000081 TGCTCACGGC TGCGTGGATA AAACCCAAAG GGAATCCAGA ATCAAGCTCG GCCTCGCAGG AGTTTATAAC TTTTGTACTT 000160
000161 TTCCCTCCCT TGTAACGAAA AGCTAATCGC AAACGTTTCT AGGAACACAT TTTTCTCCTG ATAGACGTTC CCAAGGTGGA 000240
000241 GGGCGCGAGA AGACTGGCCC GTGGGGTGAG GAGCCCCGGC CCGACCTCAG CGGCGCCACA AAGCCATGAA GAGGGGAAAT 000320
000321 AATTTTCCAA GGGGCTAAGA GTTGGACTTT CTGAAGCCAT CGAAACCTGC TGCTAGGCTC TCCCGCTGGG GTTTTGAGAG 000400
000401 AGGTAGGTGA GGAGGGATCA AACATCTCCA CCCGCTCTTG AAAGAACCGC CTTGAGCTGC GCCCAACTTC TGCAATCCCG 000480
000481 GTTTTCCCCA AGTTCAAGGG GAAGGGCCCT GGCGCCCAAT GCCCAGCCTC CCCGACAACA TCCTCGCCAG GGTGCGCCTG 000560
000561 CCCCGCGGGT CCGCTGTCCC CGGCGGGCGG CCCTGGGTTC GAGGACCCGG CTTCCCGGAG GCGCGACTCT CCAGCAAACA 000640
000641 AGCGGAGCTC CAGAAAAAAC ACCGACGCGA AGGGGGTGGA CAGAGGGTGG GAAGCCGCGG GGTTGTCGCC CACCCGGAGG 000720
000721 CGGAATGTAA CGCGCTGTGG GAAACGTGGC AGGAGCCCTG AGGCGGCGTC TTTTTCCTCA GGCGCCCGCC GAGGGTCCCG 000800
000801 CGGAAAGCCT CGCGGGCCGC CCTCGCGGCC CGCAGCCCCG CTCTGGCGCC GAGCCGGAGC CCATGCAACC TGGTTCCATC 000880
000881 CCCTGCCCCT CCCCTGTCCC CGGTGCGCCG CCCGCCAGCG AGCCTTCGAC GTGGCCGCAG GGGCGACGGG ACCACCCTCC 000960
000961 CCCGATACCC ACAGCCCCGC CATGTCTGCC TTTCCCCGGC CCGGTCTCCT CACCGCCGCT GCCGCCGCCG CTCTTCGGCC 001040
001041 GGAGATTCGG CGGCCCAGAC CGTGTCCTGG CCGGGAACTG AGGGCTCCGC CTCAACGGGC CCGCGCTGGG CAACAGGGAG 001120
001121 CGCAGCGAGC CTCGTCCGGC GCGTGCGGCT CCCGCGTCGT CGGGCGGCCA AGCGCGCGTT GAGAGGACTG GCGGGCGGAC 001200
001201 GAGCGCGCAC ACGAGTGAGC GCAGCCCCAA AGCGGCGCGC AGGGGGCTCG CGGCCCGGAA GAGGGGAGGG GCGATGACCC 001280
001281 GGGAAAGGGT TGGCGCGCGC GGGATCGGCT CGCGCGCCTG AGGGGCGGTG CCGGGGGCGG GGCTTCGCCG CGAGGCCACC 001360
001361 CCCGAGC