Difference between revisions of "ENST00000609176.1"

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Please input one-sentence summary here.
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''AIRN'' regulates genomic imprinting of a cluster of autosomal genes in cis.
  
 
==Annotated Information==
 
==Annotated Information==
  
 
===Name===
 
===Name===
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AIRN: Antisense of IGF2R non-protein coding RNA
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''AIRN''  Antisense of IGF2R long noncoding RNA
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AIR
 
AIR
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IGF2RAS
 
IGF2RAS
  
 
===Characteristics===
 
===Characteristics===
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108 kb (unspliced). Transcribed from an antisense promoter located in intron 2 of the Igf2r (insulin-like growth-factor type-2 receptor) gene<ref name="ref1"/><ref name="ref2"/>.  
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Unspliced ''AIRN''  is transcribed from an antisense promoter located in intron 2 of the Igf2r (insulin-like growth-factor type-2 receptor) gene<ref name="ref1"/><ref name="ref2"/>.  
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A number of spliced isoforms of ''AIRN''  varies in size from 500bp to ~1.4kb<ref name="ref3"/>.
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Also a number of spliced isoforms from 500bp to ~1.4kb<ref name="ref3"/>.
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''AIRN'' long noncoding RNA is Imprinte and is expressed only from paternal allele<ref name="ref1"/><ref name="ref2"/>.
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Unspliced ''AIRN''  is unstable, and is localised to the nucleus. Stable spliced isoforms of ''AIRN'' are found in nucleus and cytoplasm<ref name="ref3"/>.
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Imprinted, expressed only from paternal allele<ref name="ref1"/><ref name="ref2"/>.
 
−
 
 
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Unspliced Air is unstable, localised to the nucleus. Spliced isoforms, found in nucleus and cytoplasm, are more stable<ref name="ref3"/>.
 
  
 
===Function===
 
===Function===
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Regulates genomic imprinting of a cluster of autosomal genes (Igf2r, Slc22a2 and Slc22a3) in cis, on mouse chromosome 17 <ref name="ref4"/>.  
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''AIRN'' Regulates genomic imprinting of a cluster of autosomal genes (Igf2r, Slc22a2 and Slc22a3) in cis, on mouse chromosome 17 <ref name="ref4"/>.  
  
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Truncation of Air disrupts imprinting not only of Igf2r but also the nearby non-overlapping genes Slc22a2 and Slc22a3, leading to mice with a lower birth weight <ref name="ref4"/>.
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Truncation of ''AIRN''  disrupts imprinting not only of Igf2r but also the nearby non-overlapping genes Slc22a2 and Slc22a3, leading to mice with a lower birth weight <ref name="ref4"/>.
 +
''AIRN''  silences Igf2r through transcription alone and not via its RNA product<ref name="ref11"/><ref name="ref10"/>.
  
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Airn silences Igf2r through transcription alone and not via its RNA product<ref name="ref12"/>. Airn may regulate imprinted expression of genes within the Igf2r/Airn cluster by accumulating at promoter sites and recruiting histone modifying enzymes to epigenetically silence transcription<ref name="ref9"/>
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[[File: Airn.png |right|thumb|500px|  ''AIRN'' can silence Igf2r at any time by transcriptional overlap <ref name="ref10"/>]]
 +
''AIRN'' forms a "cloud" over the imprinted DNA locus, binding to chromatin.
  
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Air RNA forms a "cloud" over the imprinted DNA locus, binding to chromatin. Recruits G9a histone methyltransferase to epigenetically silence Slc22a3 <ref name="ref5"/>, but Igf2r is not silenced by this mechanism. Airn may induce imprinted Igf2r expression by creating an expression bias between the maternal and paternal alleles<ref name="ref9"/>.
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''AIRN'' Recruits G9a histone methyltransferase to epigenetically silence Slc22a3 <ref name="ref5"/>. Slc22a3 and Slc22a2 are only imprinted at a specific time point in placental development. Outside of this time point the ''AIRN''  "cloud"/ ''AIRN''  chromatin binding and repressive histone modification are not found on these gene loci<ref name="ref5"/>.
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Nb: Slc22a3 and Slc22a2 are only imprinted at a specific time point in placental development. Outside of this time point the Air "cloud"/ Air chromatin binding and repressive histone modification are not found on these gene loci<ref name="ref5"/>.
 
  
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Although ''AIRN''  transcription can repress Igf2r at any time, it does so less efficiently when paternal Igf2r expression is high, as in late ESC differentiation <ref name="ref9"/>. Continuous ''AIRN'' expression is needed to maintain Igf2r silencing, but only until the paternal Igf2r promoter is methylated<ref name="ref9"/>.
  
 
===Regulation===
 
===Regulation===
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Please input regulation information here.
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The unmethlyated imprint control region (ICR) acts as promoter for ''AIRN'', which then silence all imprinted genes in the cluster on that parental allele<ref name="ref4"/><ref name="ref11"/>.
  
 
===Expression===
 
===Expression===
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[[File: Airn.png |right|thumb|500px|  Airn can silence Igf2r at any time by transcriptional overlap [<ref name="ref12"/>]]]
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''AIRN'' is expressed during embryonic development, in the placenta<ref name="ref1"/><ref name="ref2"/>, adult brain, lung and heart<ref name="ref3"/>.
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Expressed during embryonic development, in the placenta<ref name="ref1"/><ref name="ref2"/>, adult brain, lung and heart<ref name="ref3"/>.
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''AIRN'' is also expressed in glial cells of the developing brain but not in neurons, explains lack of Igf2r imprinting in these neurons<ref name="ref6"/>.
 +
In human, IGF2R gene is predominantly nonimprinted and ''AIRN'' is typically not expressed; however, expression was identified in association with 16–40% of Wilms’ tumors<ref name="ref7"/>.
 +
Bovine ''AIRN'' is not expressed in blastocyst-stage embryos. It is expressed in an increasing proportion of embryos around the time of maternal recognition of pregnancy and is expressed following implantation <ref name="ref8"/>.
  
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Expressed in glial cells of the developing brain but not in neurons, explains lack of Igf2r imprinting in these neurons<ref name="ref6"/>.
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===Disease===
 +
*Wilms’tumors <ref name="ref7"/>.
  
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Airn expression is necessary to initiate paternal-specific silencing of the Igf2r gene, which is followed by gain of a somatic DNA methylation imprint on the silent Igf2r promoter<ref name="ref11"/><ref name="ref10"/>. So continuous Airn expression is needed to maintain Igf2r silencing, but only until the paternal Igf2r promoter is methylated<ref name="ref10"/>. Igf2r silencing does not require any part of the Airn lncRNA, but only transcription through the Igf2r promoter.
 
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In the human, the IGF2R gene is predominantly nonimprinted and AIRN is typically not expressed; however, expression was identified in association with 16–40% of Wilms’ tumors<ref name="ref9"/>. Additionally, while human AIRN ncRNA is not expressed in most tissues, a competent IGF2R/AIRN imprinting cluster has been identified<ref name="ref9"/>.
 
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In mice, loss of the maintenance DNA methylation enzyme, de novo methyltransferase 1 (Dnmt1), resulted in reduced expression of Igf2r and increased expression of Airn<ref name="ref8"/><ref name="ref9"/>.
 
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===Conservation===
 
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All characterisation been carried out in mouse. Recently Air has been identified in human<ref name="ref7"/>.
 
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You can also add sub-section(s) at will.
 
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
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*CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, 1090 Vienna, Austria.
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Institute of Molecular Pathology, Vienna, Austria<ref name="ref2"/>.
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*Department of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands.
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*Institute of Molecular Pathology, Vienna.
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*The Netherlands Cancer Institute (H5), Amsterdam.
  
 
==References==
 
==References==
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<ref name="ref6">Yamasaki, Y., et al. (2005). "Neuron-specific relaxation of Igf2r imprinting is associated with neuron-specific histone modifications and lack of its antisense transcript Air." Hum Mol Genet 14(17): 2511-2520. </ref>
 
<ref name="ref6">Yamasaki, Y., et al. (2005). "Neuron-specific relaxation of Igf2r imprinting is associated with neuron-specific histone modifications and lack of its antisense transcript Air." Hum Mol Genet 14(17): 2511-2520. </ref>
 
<ref name="ref7"> Yotova, I. Y., et al. (2008). "Identification of the human homolog of the imprinted mouse Air non-coding RNA." Genomics 92(6): 464-473. </ref>
 
<ref name="ref7"> Yotova, I. Y., et al. (2008). "Identification of the human homolog of the imprinted mouse Air non-coding RNA." Genomics 92(6): 464-473. </ref>
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<ref name="ref8"> Brosens, I., et al. (2013). "Defective myometrial spiral artery remodelling as a cause of major obstetrical syndromes in endometriosis and adenomyosis." Placenta 34(2): 100-105.</ref>
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<ref name="ref8">Farmer, W. T., et al. (2013). "Expression of antisense of insulin-like growth factor-2 receptor RNA non-coding (AIRN) during early gestation in cattle." Anim Reprod Sci 138(1-2): 64-73.</ref>
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<ref name="ref9">Farmer, W. T., et al. (2013). "Expression of antisense of insulin-like growth factor-2 receptor RNA non-coding (AIRN) during early gestation in cattle." Anim Reprod Sci 138(1-2): 64-73.</ref>
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<ref name="ref9">Santoro, F., et al. (2013). "Imprinted Igf2r silencing depends on continuous Airn lncRNA expression and is not restricted to a developmental window." Development 140(6): 1184-1195. </ref>
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<ref name="ref10">Santoro, F., et al. (2013). "Imprinted Igf2r silencing depends on continuous Airn lncRNA expression and is not restricted to a developmental window." Development 140(6): 1184-1195. </ref>
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<ref name="ref10"> Santoro, F. and F. M. Pauler (2013). "Silencing by the imprinted Airn macro lncRNA: transcription is the answer." Cell Cycle 12(5): 711-712.</ref>
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<ref name="ref11"> Kobayashi, H., et al. (2013). "Epigenetic and transcriptional features of the novel human imprinted lncRNA GPR1AS suggest it is a functional ortholog to mouse Zdbf2linc." Epigenetics 8(6): 635-645.</ref>
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<ref name="ref11">Latos, P.A., Pauler, F.M., Koerner, M.V., Senergin, H.B., Hudson, Q.J., Stocsits, R.R., Allhoff, W., Stricker, S.H., Klement, R.M., Warczok, K.E. et al. (2012) Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing. Science, 338, 1469-1472.</ref>
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<ref name="ref12"> Santoro, F. and F. M. Pauler (2013). "Silencing by the imprinted Airn macro lncRNA: transcription is the answer." Cell Cycle 12(5): 711-712.</ref>
 
 
</references>
 
</references>
 +
 +
sequence = 100271873
  
  
−
{{basic|
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===Sequence===
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tID = ENST00000609176.1|
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>gi|100271873|ref|NR_047511.1|Homo sapiens antisense of IGF2R non-protein coding RNA (AIRN), transcript variant 1, long non-coding RNA
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source = Gencode19|
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<dnaseq>GAAAGCACACACCATGCTGGAAAGGAGAGAGAAGGAAAAACCCACTCCTAGCCACACAATGGTGGATCTT
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same = NONHSAT115887|
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AATATCCAATGAAAAGAAAAAATTCTAATCTAGGTCACACACAATAACCCAATATAAAATGAACAAGACA
−
classification = intronic(AS)|
+
TGATCAAGCAAATCAAAGAAAAATGTACATCAATAAATATGTTAAGACATGTTCAATGTTACTGGTGATC
−
length = 1489 nt|
+
AAGGAATTTCAAATCAAGACCAAAATGGTATACCATTTACAACCATCCCTCTGGCAAAAATTAAATACCA
−
location = chr6-:160424323..160428696|
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AGTGTCAGCCATGAGGTGGCTCAGAGCTCTCCTGTATTGCTAGTGAAATGTTAACTGGTTCAACAACTAT
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number = 3|
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GTAAGTCAGCAGGACATTATGTCCTAAAATTGAACATTCGTGTGCCCTATGACCTAGTTACTCCACTCAG
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exons = 160424323..160424419,160425983..160426190,160427513..160428696|
+
GTCTATATAAAAGAGAAAACCATGCACATATGGAACATGCACAGCAGAAAATGTGAAAGAATATCTCATG
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context = <html><div align="center">
+
CTCTTCAGAAGAGCAAAAACCTGGAAATGACCAAAGGCCCATCAACAGAAGAGTGGATAAATTAACTTCA
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<iframe src="http://lncrna.big.ac.cn/view/?data=species/human&loc=chr6:160424323..160428696&tracklist=0&overview=0&tracks=DNA,RefGene,lncRNA" style=" border-width:0 " width="100%" height="250" scrolling="yes"></iframe>
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GCAGATCCACACAAAATATCACAGCAGTCAGAAGGAATAAACCACAGTGAAAAACAACAATACGGTTGAT
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</div></html>|
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TTTTAGTAATATTACTGTTATCACTTAAGTCCCAAAAAGTTACACATAGGAAGATAACTTAGTTGCAAAC
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sequence = <dnaseq>GAAAGCACACACCATGCTGGAAAGGAGAGAGAAGGAAAAACCCACTCCTAGCCACACAATGGTGGATCTTAATATCCAATGAAAAGAAAAAATTCTAATCTAGGTCACACACAATAACCCAATATAAAATGAACAAGACATGATCAAGCAAATCAAAGAAAAATGTACATCAATAAATATGTTAAGACATGTTCAATGTTACTGGTGATCAAGGAATTTCAAATCAAGACCAAAATGGTATACCATTTACAACCATCCCTCTGGCAAAAATTAAATACCAAGTGTCAGCCATGAGGTGGCTCAGAGCTCTCCTGTATTGCTAGTGAAATGTTAACTGGTTCAACAACTATGTAAGTCAGCAGGACATTATGTCCTAAAATTGAACATTCGTGTGCCCTATGACCTAGTTACTCCACTCAGGTCTATATAAAAGAGAAAACCATGCACATATGGAACATGCACAGCAGAAAATGTGAAAGAATATCTCATGCTCTTCAGAAGAGCAAAAACCTGGAAATGACCAAAGGCCCATCAACAGAAGAGTGGATAAATTAACTTCAGCAGATCCACACAAAATATCACAGCAGTCAGAAGGAATAAACCACAGTGAAAAACAACAATACGGTTGATTTTTAGTAATATTACTGTTATCACTTAAGTCCCAAAAAGTTACACATAGGAAGATAACTTAGTTGCAAACACACAGATTTTTTTTTTTTGGAAATTAGATACAGTAAAAAAAAAAAAAAATTAATACAAGATGGGAGCTAATGATTACTCCTAGCGAAAAGTGAAAGAGGAAAGAGTAAAACAGATAGTGGTTACTGCCAAGATCCTAGATTTTTGTTGGAAACAGTGCTCGCTACATTATACAAAACAACTAATTTTTTAAAAGGAAAAAGGTTAGTCTATGGATTAACACATGGCTCACTGTACGTGCCATGAGCTCGGGATGATAGCTGATCCAATCCCATATACACCTGAAGTCTTGGGGCAGGGGCGGACCACGAGTCAGCGCGCCCCCACAGCACCAGGCTGATGGCCCCGTGGTTCTTCTGCCTGCAAGCAAAAGTCAGCCACGATCATCCTTCTCCATCTGCAACACCCAGGGTCTTTTCTAAAACTTTTGCCCAGTCGGCTCAGCCAAAAGGACACAGAAGCCCTGCAGAGGCTCTGAAACCAAGCTGCCATGGCTCCCAGTTCGACATCATGTCACCGGAGCCTGCACAGATGAACCAAGCTTGCAACACGGGAGGAACCTAAGCGCTGGACTGAGGAGCGGGACTGAAATAAGAAGCGCACACCACATGGCAAGATCCAGGATCCAATCAGATAGAGCCCTGGTGTCATCTAATTGCAAGATCCAATCAGAACACACCTCATTACCTTATGGTATTGCCAGACTATCAGGAGAGACCATAAATCAGATAAAAACATGAGTTGTATCCAAAACTCAACCAGGTATCATCTTAAATATCCAACAGAATTC</dnaseq>|
+
ACACAGATTTTTTTTTTTTGGAAATTAGATACAGTAAAAAAAAAAAAAAATTAATACAAGATGGGAGCTA
−
}}
+
ATGATTACTCCTAGCGAAAAGTGAAAGAGGAAAGAGTAAAACAGATAGTGGTTACTGCCAAGATCCTAGA
−
[[Category:Intronic(AS)]][[Category:ENSG00000268257.2]][[Category:NONHSAG045306]]
+
TTTTTGTTGGAAACAGTGCTCGCTACATTATACAAAACAACTAATTTTTTAAAAGGAAAAAGGTTAGTCT
 +
ATGGATTAACACATGGCTCACTGTACGTGCCATGAGCTCGGGATGATAGCTGATCCAATCCCATATACAC
 +
CTGAAGTCTTGGGGCAGGGGCGGACCACGAGTCAGCGCGCCCCCACAGCACCAGGCTGATGGCCCCGTGG
 +
TTCTTCTGCCTGCAAGCAAAAGTCAGCCACGATCATCCTTCTCCATCTGCAACACCCAGGGTCTTTTCTA
 +
AAACTTTTGCCCAGTCGGCTCAGCCAAAAGGACACAGAAGCCCTGCAGAGGCTCTGAAACCAAGCTGCCA
 +
TGGCTCCCAGTTCGACATCATGTCACCGGAGCCTGCACAGATGAACCAAGCTTGCAACACGGGAGGAACC
 +
TAAGCGCTGGACTGAGGAGCGGGACTGAAATAAGAAGCGCACACCACATGGCAAGATCCAGGATCCAATC
 +
AGATAGAGCCCTGGTGTCATCTAATTGCAAGATCCAATCAGAACACACCTCATTACCTTATGGTATTGCC
 +
AGACTATCAGGAGAGACCATAAATCAGATAAAAACATGAGTTGTATCCAAAACTCAACCAGGTATCATCT
 +
TAAATATCCAACAGAATTC</dnaseq>

Latest revision as of 05:40, 14 November 2018

AIRN regulates genomic imprinting of a cluster of autosomal genes in cis.

Annotated Information

Name

AIRN Antisense of IGF2R long noncoding RNA AIR IGF2RAS

Characteristics

Unspliced AIRN is transcribed from an antisense promoter located in intron 2 of the Igf2r (insulin-like growth-factor type-2 receptor) gene[1][2]. A number of spliced isoforms of AIRN varies in size from 500bp to ~1.4kb[3]. AIRN long noncoding RNA is Imprinte and is expressed only from paternal allele[1][2]. Unspliced AIRN is unstable, and is localised to the nucleus. Stable spliced isoforms of AIRN are found in nucleus and cytoplasm[3].

Function

AIRN Regulates genomic imprinting of a cluster of autosomal genes (Igf2r, Slc22a2 and Slc22a3) in cis, on mouse chromosome 17 [4].

Truncation of AIRN disrupts imprinting not only of Igf2r but also the nearby non-overlapping genes Slc22a2 and Slc22a3, leading to mice with a lower birth weight [4]. AIRN silences Igf2r through transcription alone and not via its RNA product[5][6].

AIRN can silence Igf2r at any time by transcriptional overlap [6]

AIRN forms a "cloud" over the imprinted DNA locus, binding to chromatin.

AIRN Recruits G9a histone methyltransferase to epigenetically silence Slc22a3 [7]. Slc22a3 and Slc22a2 are only imprinted at a specific time point in placental development. Outside of this time point the AIRN "cloud"/ AIRN chromatin binding and repressive histone modification are not found on these gene loci[7].

Although AIRN transcription can repress Igf2r at any time, it does so less efficiently when paternal Igf2r expression is high, as in late ESC differentiation [8]. Continuous AIRN expression is needed to maintain Igf2r silencing, but only until the paternal Igf2r promoter is methylated[8].

Regulation

The unmethlyated imprint control region (ICR) acts as promoter for AIRN, which then silence all imprinted genes in the cluster on that parental allele[4][5].

Expression

AIRN is expressed during embryonic development, in the placenta[1][2], adult brain, lung and heart[3]. AIRN is also expressed in glial cells of the developing brain but not in neurons, explains lack of Igf2r imprinting in these neurons[9]. In human, IGF2R gene is predominantly nonimprinted and AIRN is typically not expressed; however, expression was identified in association with 16–40% of Wilms’ tumors[10]. Bovine AIRN is not expressed in blastocyst-stage embryos. It is expressed in an increasing proportion of embryos around the time of maternal recognition of pregnancy and is expressed following implantation [11].

Disease

  • Wilms’tumors [10].


Labs working on this lncRNA

  • CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, 1090 Vienna, Austria.
  • Department of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands.
  • Institute of Molecular Pathology, Vienna.
  • The Netherlands Cancer Institute (H5), Amsterdam.

References

  1. ↑ 1.0 1.1 1.2 Lyle, R., et al. (2000). "The imprinted antisense RNA at the Igf2r locus overlaps but does not imprint Mas1." Nat Genet 25(1): 19-21.
  2. ↑ 2.0 2.1 2.2 Wutz, A., et al. (1997). "Imprinted expression of the Igf2r gene depends on an intronic CpG island." Nature 389(6652): 745-749.
  3. ↑ 3.0 3.1 3.2 Seidl, C. I., et al. (2006). "The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export." EMBO J 25(15): 3565-3575.
  4. ↑ 4.0 4.1 4.2 Sleutels, F., et al. (2002). "The non-coding Air RNA is required for silencing autosomal imprinted genes." Nature 415(6873): 810-813.
  5. ↑ 5.0 5.1 Latos, P.A., Pauler, F.M., Koerner, M.V., Senergin, H.B., Hudson, Q.J., Stocsits, R.R., Allhoff, W., Stricker, S.H., Klement, R.M., Warczok, K.E. et al. (2012) Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing. Science, 338, 1469-1472.
  6. ↑ 6.0 6.1 Santoro, F. and F. M. Pauler (2013). "Silencing by the imprinted Airn macro lncRNA: transcription is the answer." Cell Cycle 12(5): 711-712.
  7. ↑ 7.0 7.1 Nagano, T., et al. (2008). "The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin." Science 322(5908): 1717-1720.
  8. ↑ 8.0 8.1 Santoro, F., et al. (2013). "Imprinted Igf2r silencing depends on continuous Airn lncRNA expression and is not restricted to a developmental window." Development 140(6): 1184-1195.
  9. ↑ Yamasaki, Y., et al. (2005). "Neuron-specific relaxation of Igf2r imprinting is associated with neuron-specific histone modifications and lack of its antisense transcript Air." Hum Mol Genet 14(17): 2511-2520.
  10. ↑ 10.0 10.1 Yotova, I. Y., et al. (2008). "Identification of the human homolog of the imprinted mouse Air non-coding RNA." Genomics 92(6): 464-473.
  11. ↑ Farmer, W. T., et al. (2013). "Expression of antisense of insulin-like growth factor-2 receptor RNA non-coding (AIRN) during early gestation in cattle." Anim Reprod Sci 138(1-2): 64-73.

sequence = 100271873


Sequence

>gi|100271873|ref|NR_047511.1|Homo sapiens antisense of IGF2R non-protein coding RNA (AIRN), transcript variant 1, long non-coding RNA

000001 GAAAGCACAC ACCATGCTGG AAAGGAGAGA GAAGGAAAAA CCCACTCCTA GCCACACAAT GGTGGATCTT AATATCCAAT 000080
000081 GAAAAGAAAA AATTCTAATC TAGGTCACAC ACAATAACCC AATATAAAAT GAACAAGACA TGATCAAGCA AATCAAAGAA 000160
000161 AAATGTACAT CAATAAATAT GTTAAGACAT GTTCAATGTT ACTGGTGATC AAGGAATTTC AAATCAAGAC CAAAATGGTA 000240
000241 TACCATTTAC AACCATCCCT CTGGCAAAAA TTAAATACCA AGTGTCAGCC ATGAGGTGGC TCAGAGCTCT CCTGTATTGC 000320
000321 TAGTGAAATG TTAACTGGTT CAACAACTAT GTAAGTCAGC AGGACATTAT GTCCTAAAAT TGAACATTCG TGTGCCCTAT 000400
000401 GACCTAGTTA CTCCACTCAG GTCTATATAA AAGAGAAAAC CATGCACATA TGGAACATGC ACAGCAGAAA ATGTGAAAGA 000480
000481 ATATCTCATG CTCTTCAGAA GAGCAAAAAC CTGGAAATGA CCAAAGGCCC ATCAACAGAA GAGTGGATAA ATTAACTTCA 000560
000561 GCAGATCCAC ACAAAATATC ACAGCAGTCA GAAGGAATAA ACCACAGTGA AAAACAACAA TACGGTTGAT TTTTAGTAAT 000640
000641 ATTACTGTTA TCACTTAAGT CCCAAAAAGT TACACATAGG AAGATAACTT AGTTGCAAAC ACACAGATTT TTTTTTTTTG 000720
000721 GAAATTAGAT ACAGTAAAAA AAAAAAAAAA TTAATACAAG ATGGGAGCTA ATGATTACTC CTAGCGAAAA GTG