Difference between revisions of "SH3PXD2A-AS1"
(→Annotated Information) |
|||
| Line 6: | Line 6: | ||
===Characteristics=== | ===Characteristics=== | ||
| − | ''SH3PXD2A-AS1'' has a genomic location of 10q24.33 and has an exon count of 3. | + | ''SH3PXD2A-AS1'' has a genomic location of 10q24.33 and has an exon count of 3 (HGNC). |
===Function=== | ===Function=== | ||
Revision as of 06:51, 14 December 2018
SH3PXD2A-AS1 acts as an oncogene in colorectal cancer (CRC), and may be a promising diagnostic or therapeutic target in patients with CRC.
Contents
Annotated Information
Name
SH3PXD2A-AS1: SH3PXD2A antisense RNA 1 (HGNC nomenclature).
Characteristics
SH3PXD2A-AS1 has a genomic location of 10q24.33 and has an exon count of 3 (HGNC).
Function
Cell viability examination, colony-formation experiments, ethynyl deoxyuridine (Edu) assays and flow cytometry have shown the roles of SH3PXD2A-AS1 in CRC proliferation, cell cycle regulation, and apoptosis [1]. SH3PXD2A-AS1 knockdown inhibited CRC cells proliferation, migration and invasion in vitro, and suppressed tumorigenesis in vivo [1].
This expression pattern of SH3PXD2A-AS1 in CRC helps to fully understand the state of CRC patients. SH3PXD2A-AS1 may be a diagnostic biomarker and prognostic indicator for patients with CRC [1].
Regulation
SH3PXD2A-AS1 could epiqenetically repress P57 and KLF2 expression through interaction with EZH2. Overexpression of P57 or KLF2 suggests hampering of SH3PXD2A-AS1-mediated oncogenesis. Importantly, the expression of SH3PXD2A-AS1 has an inverse correlation with the expression of P57 and KLF2 in CRC tissue samples
Diseases
- Colorectal Cancer (CRC) [1]
Expression
SH3PXD2A-AS1 has been shown to be significantly upregulated in CRC tissues and cell lines, and overexpression of SH3PXD2A-AS1 is correlated with tumor size, TNM stage, and lymph node metastasis in patients with CRC [1].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-GCCTCTTTACAGCGTTCCCT - 3' | 5'-CCTGAGGATACGTTCGTGGG- 3' [1] |
Labs working on this lncRNA
- The Second Clinical Medical College of Nanjing Medical University, Nanjing, China. [1]
- Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, China. [1]
- Department of Oncology, Second Hospital of Nanjing, Nanjing, China. [1]
References
Sequence
>gi|100505839|ref|NR_038940.1| Homo sapiens SH3PXD2A antisense RNA 1 (SH3PXD2A-AS1), long non-coding RNA
000081 ATTGCCTTGC ATCCCTCATT GGGAGGCCTT TGGTGGCCAT GTGCCCTCTC TCTCCGGCCT CCGTTGTTTC CCTCCCAGAA 000160
000161 TCTGCCACCA TTGATAATGA TCTTCTGAAC CTGCCTCTTT ACAGCGTTCC CTAAGGACAG AATGCAAGCT TCTTGCAGCC 000240
000241 AGTGAAGCCC ACGAACGTAT CCTCAGGGCC CAGCACAAGG ACGGGCACAC AGGGACCCCG ACCGCGACTT GCTGAAAGAT 000320
000321 ACTCATGCTG AGGGAAGCCA GCTTCCATGT TGTAAGGATG CTGAAGCAGC ACTGTGGAGA TCCATGTGGC AAGGAGCTGA 000400
000401 GGCCTCTGCC AGCAGCCAGC GCCAGTCTGC CAGCGAGATG AGCAAGCCGT CTTGCAGCCA ATTCAACGAA TTGTTATCTC 000480
000481 TGGACCACTG GGCTCACATC GTGAACAGCT GCTCCACCCT CTGTCTTTCA CCAGCAAGTT TTTCTGGTAA CAAGACCACA 000560
000561 TAGGTCTCAC TTAAGCCATC TTCACCAACT ACCAACTATT AAACTGGTTA ATTCGTCTTT CAGATCAGAA ACAAGAGGCC 000640
000641 AGGAGTAAAA ACTGTGCCAA TCAGAGAAGA ATTACCTGGC CACCAAATTA CCAGTCACTC ACTCATCTCA ACTCTCCTCC 000720
000721 CTGGCTCCTT TACTTTGAAG CTTAGGTCTG CAATGGTTAT TTTATATAGA AGAGCAAAAT ATGCTCCTTT CAAATTTCAC 000800
000801 TTTTTTTTCT TTTTTGAAAT TAAAACCAAT TTTGTTTAGA GACAGGGTCG CACTCTGCCA CCCAGGATGG AGTGCAGTGG 000880
000881 CATGATCATA GCTCGCTGTA ACCCCAAACT CCTGGGCTCA AGTGATCCTC ACACCTCAGC CTCCCGAGTA GCTGGTACAG 000960
000961 GAGTGTGCCA CCATGCTTGG CTTCACTTTA TTTTGTTGAC TTAACTTTTT CCAGAGACAT CTTACAAGAG CCTGTTTTCC 001040
001041 ATCCTAACAG TACGACGTAA CTCCAGACAT AGGCTTTAGA CGTTCTCATG CCACCCTATC TTCAAAACCA CAGAGAGTTC 001120
001121 ATGAGCCAGT CTTGCCCATC TCCAATCAGG GAACTTCTAA AATAAAAATC TTAGCAATCT CCTTGGCCCA AAACTTCACC 001200
001201 CCATCTTGGA AGGGAGGGGA GAGAGAATGT TCTGATCTAT ATCTGATGAG GGCGTGTGGT TGGGACCTGA GCATCCTCCT 001280
001281 GGTTGGGCTA GTGATGGGGA GAGAGGGCTG TTACTCACGA CTCCCTCCCA CAGAATACCA GAAACAGGCA GGCAGCTCAG 001360
001361 GTGTATGTAA GGATGTGAGG CCAAGAAACC AGCCCTCACC AAGTTACCCC TGTAAATCCT TGTCTCCCCA TGCACCTCTA 001440
001441 CTTTGAGTCA GAAATGGATT CATTGCAGGC TCAGTTGTTT GTATTATGTG AATGAACTGA ACGTAACCAA GCACCAAGAG 001520
001521 AGCCCTAAAG ACACAGTAGA CCTCCTGTAG AAGGGCTCTG ATGGACCTTC AAACATTGCT TCTCCAACTT TATGGTGCAC 001600
001601 ACAAATCACC TGTGCATGTT AAAATGCAGA CGGTGACTTA CAGATCTTGG GGGAGGCCAA GTGTCTGCAT TTCCAACAAG 001680
001681 CTCCCAGATG ATGGCCACAC TGCTGATCTG AGGACCACAT TTTGAACAGC AATCCTTAAA ACACACAAGG CTGTGGGACC 001760
001761 GCACTCCTGA GAAGAGACCT CACTCTTGGA AGGGATTCTA GGAGGCTGCA TTCAAACCAT CCCAGAGACA GCTCACAATT 001840
001841 TTGTTAGGGA CCTGATACTA AAAATTACCA AATCACAATC GTGTGCTGAG GGCTTACTTT GTGCTTTTCA GATGACATTT 001920
001921 CATTTGATGC TCTCAATAAC CCTGAAGGGA TTTCAGAGAT GGGGATTATG ATGGAGCTTC AGAATTAAAC TGATTAAAAC 002000
002001 TGGCCCAGAG TCACACAACT ATG
