Difference between revisions of "ENST00000500949.2"
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| − | + | ''CYRANO''- long noncoding RNA required for proper embryonic development | |
==Annotated Information== | ==Annotated Information== | ||
| − | ===Name=== | + | ===Approved Symbol=== |
| − | + | ''CYRANO'' | |
| + | ===Approved Name=== | ||
| + | ''CYRANO''. | ||
| + | linc-oip5 <ref name="ref1" />. | ||
| + | 1700020I14Rik <ref name="ref1" />. | ||
===Characteristics=== | ===Characteristics=== | ||
| − | + | 8.2-kb ''CYRANO'' molecule is a long intergenic ncRNA (lincRNA) transcribed in mouse ESCs <ref name="ref1" /><ref name="ref2" /><ref name="ref3" /><ref name="ref4" />. | |
| − | + | ''CYRANO'' shares unique sequence complementarity with the differentiation-associated microRNA <ref name="ref4" />. | |
| − | + | ''CYRANO'' distribution in the cell could indicate that nuclear and cytoplasmic ''CYRANO'' pools that have distinct functions or that it interacts with proteins that shuttle from the nucleus to the cytoplasm <ref name="ref4" />. | |
| − | + | ''CYRANO'' posseses an almost complete binding site to mir-7 in mouse ESCs and this binding site is conserved across vertebrates <ref name="ref5" />. | |
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| − | = | ||
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===Function=== | ===Function=== | ||
| − | + | lincRNA ''CYRANO'' is required for proper embryonic development <ref name="ref3" />. | |
| − | + | Depletion of ''CYRANO'' results in disarray in the pluripotent gene expression signature and defects in self-renewal maintenance <ref name="ref4" />. | |
| + | ''CYRANO'' is one factor that presents a barrier to lineage commitment in conditions of suboptimal fluctuations in Nanog levels <ref name="ref4" />. | ||
| + | ''CYRANO'' function as a sponge of RBPs from mRNAs <ref name="ref6" />. | ||
| + | ''CYRANO'' in various systems include anti-proliferative functions and play roles in organogenesis/embryonic development <ref name="ref4" />. | ||
| − | + | ===Regulation=== | |
| + | mir-7 regulates ''CYRANO'' <ref name="ref3" />. | ||
| − | * | + | ===Disease=== |
| + | *Astrocytoma <ref name="ref8" /> | ||
| + | *Glioma <ref name="ref9" /> | ||
| + | *Hepatoblastoma <ref name="ref10" /> | ||
| − | |||
| − | |||
===Expression=== | ===Expression=== | ||
| − | + | ''CYRANO'' is expressed in zebrafish nervous system. Brain and notochord at 24 hours post fertilization (hpf), expression at 72 hpf also includes zebrafish spinal cord. Mouse and human homolgoues are widely expressed in adult and developing tissues and cell lines including the brain. RNA stability half-life of mouse homologue was 3-4 hr in neuroblastoma cells, close to lncRNA median <ref name="ref7" /> | |
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==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | + | *Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA | |
| + | *Department of General Surgery, the First Hospital of Jilin University, Changchun, 130021, China | ||
| + | *Department of General Surgery, the First Hospital of Jilin University, Changchun, 130021, China | ||
| + | *Laboratory of Genetics and Genomics, National Institute on Aging-Intramural Research Program, NIH, Baltimore, MD 21224, USA | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1"> Chew G.L., Pauli A., Rinn J.L., Regev A., Schier A.F., Valen E. Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs. Development. 2013;140:2828–2834 | ||
| + | </ref>(1) | ||
| + | <ref name="ref2"> Guttman M., Garber M., Levin J.Z., Donaghey J., Robinson J., Adiconis X., Fan L., Koziol M.J., Gnirke A., Nusbaum C. Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs. Nat. Biotechnol. 2010;28:503–510 | ||
| + | </ref>(2) | ||
| + | <ref name="ref3"> Ulitsky I., Shkumatava A., Jan C.H., Sive H., Bartel D.P. Conserved function of lincRNAs in vertebrate embryonic development despite rapid sequence evolution. Cell. 2011;147:1537–1550. | ||
| + | </ref>(3) | ||
| + | <ref name="ref4"> Smith KN, Starmer J, Miller SC, Sethupathy P & Magnuson T. Long Noncoding RNA Moderates MicroRNA Activity to Maintain Self-Renewal in Embryonic Stem Cells[J]. Stem Cell Rep. 2017, 9(1):108-121. | ||
| + | </ref>(4) | ||
| + | <ref name="ref5"> Sigova A.A., Mullen A.C., Molinie B., Gupta S., Orlando D.A., Guenther M.G., Almada A.E., Lin C., Sharp P.A., Giallourakis C.C. Divergent transcription of noncoding RNA/mRNA gene pairs in embryonic stem cells. Proc. Natl. Acad. Sci. USA. 2013;110:2876–2881. | ||
| + | </ref>(5) | ||
| + | <ref name="ref6"> Kim J., Abdelmohsen K., Yang X., De S., Grammatikakis I., Noh J.H., Gorospe M. LncRNA OIP5-AS1/cyrano sponges RNA-binding protein HuR. Nucleic Acids Res. 2016;44:2378–2392 | ||
| + | </ref>(6) | ||
| + | <ref name="ref7"> Clark MB, Johnston RL, Inostroza-Ponta M, Fox AH, Fortini E, Moscato P et al. Genome-wide analysis of long noncoding RNA stability[J]. Genome Res. 2012, 22(5):885-898. | ||
| + | </ref>(7) | ||
| + | <ref name="ref8"> Zhi F, Wang Q, Xue L, Shao NY, Wang R, Deng DN et al. The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma[J]. Plos One. 2015, 10(8). | ||
| + | </ref>(8) | ||
| + | <ref name="ref9"> Hu GW, Wu L, Kuang W, Chen Y, Zhu XG, Guo H et al. Knockdown of linc-OIP5 inhibits proliferation and migration of glioma cells through down-regulation of YAP-NOTCH signaling pathway[J]. Gene. 2017, 610(24-31 | ||
| + | </ref>(9) | ||
| + | <ref name="ref10"> Zhang Z, Liu F, Yang F & Liu YH. Kockdown of OIP5-AS1 expression inhibits proliferation, metastasis and EMT progress in hepatoblastoma cells through up-regulating miR-186a-5p and down-regulating ZEB1[J]. Biomed Pharmacother. 2018. | ||
| + | </ref>(10) | ||
| + | </references> | ||
| − | + | ===Sequence=== | |
| − | + | >gi|729082|ref|NR_026757.2|Homo sapiens OIP5 antisense RNA 1 (OIP5-AS1), transcript variant 1, long non-coding RNA | |
| − | + | <dnaseq>AGGGTTGGCCACCTTGAGAAGCTGCGAAGATGGCGGAGTAAGGCGTGCCGCTGCAAACTGGCCTCTGGGC | |
| − | + | CGGGGGCGAGCAGCCCCCGGGAGGCCGAGTGCATCTGTTGGACCGTGCGAGGAGAAGAAAAAAAATATCG | |
| − | + | GCCAGAGGAGAGGAACTAACCGAACATTCTTCCTCCCTACCTTATAGAGGGGAGTGGTCATCTACACTAA | |
| − | + | AGCAAAGTGTTAGGCGCGTCCGTGCGAAGAGACCACCAAACAGGCTTTGTGTTCCTTATCACAGGAAGAA | |
| − | + | AATTTTCCTTGACCTTTAGGTGCTTTTATATTCATCTCAAAAACAAAATTCTGAACTCAGGACTTGGCAA | |
| − | + | GTGTCTCTATGTTGTCTCCTAGAGTGGGTAGTCCTGCTTCTTTTACCCAGTTACTTTCCCGTTTTTGAAA | |
| − | + | TGTGGCTATCACTTCTCTACACATTACCTCCATGATTTGGAATGGAAAAGGCCACTTTTCTTTTTGTTCT | |
| − | + | GCCTCTCAAATTCAACACAGAGGAGCTCCTAGGATTCCAGTTATCCTGCTAACATCTCCAGGAAGAAAGA | |
| − | + | AGCAACTCACATGGGTCTTTTGCTGTTTGCTTAATTATAAAGACATCATTTTGCAAGCAGAAGGCTGAGT | |
| − | + | TTCATTTGAAACAGGTGCTTAGGTGGTGGTATTTGTGAATACTTTTCATTCCAAGCAAGAAGACTAAAGA | |
| − | + | AGTAGCAAGTATGAATGACTTCAGGGTTTAAAAAAAATGTCTTCCAGTTTCAGCCACTACCATGATAAGC | |
| − | + | ACAGTTGAGACTGCAGCAGTAAATTCCAAATATGTGTTTCTAATTTGACGTGAAAGATACTAAAAATTTA | |
| − | + | TATTTGTATATTTAAATCCTGGCTCATCCTGTGACATAGATTTACTGAATAGGAACAAAGGCCCAATTTT | |
| + | AAACAAAAACCTAGGCCGGGTGTGGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCAAGGCAGGC | ||
| + | AAATCACCTGAGGTTGGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAATA | ||
| + | GAAAATTAGCCAGGTGTGGTGGTAGATACGTGTAATCCCAGCTACTCGTGAGGCTGAGGCAGGAGAATTG | ||
| + | CTTGAATCCGGGAGGCGGAGGTTGCAGTGAACTGAGATTGCACCACTGCACTCCAGCCTGGGCGACAGAG | ||
| + | TGAGACTCCGTCTCAGAAAAATAAAAAACAACTTTGGACATAGAGGAGTTGGGGGCAGAGGGGGGAGGAT | ||
| + | GAGAGAATTTTCCATGTAACTCCTTTTCCTTAAGAAAAAGAAGCAAAAATCCCTCATGCAGTGCCATCTG | ||
| + | ACTTTATGGTGTTTCACATTATATAGTTACTTTTTTTAATAAACGCATAAGATTAACTCTTCCTGCAACC | ||
| + | CAAGGTGGATACTTGGATGTTTGATTTTTATTTATTTATTTATTTATTTTTTGAGGCAGTCTCTCTCTGT | ||
| + | TGCCCAGGCTGGAGTGATGCGTGATTTCTGCTCACTGCAGTCTCTCCCACCCGGGTTCAAGCGATTCTCC | ||
| + | TGCCTCAGCCTCCCAAGTAGCTAGGATTACAGGCGCGCACCACCACGCTCAGCCTGATTTCTAGGCTGAA | ||
| + | AAGGCTGGGACTCAGGTACTTTCCCCAGTTGGGACTGAACTTTCTCATCAGAGAATGGGCCTCAGAAGCA | ||
| + | ATGTTCCCAAACTTGTGGTTGGGTCATCGTGGAAAAGAAACCTCAAATAAGAAAAATAATTACAATAAGA | ||
| + | AATGGATTTTCTTTTTTCCCACAACAGTTATACATTATAAAGAACAGACTGTCGTAGAAAACTGTCTTTG | ||
| + | CTTCCAAATCAGCAGAGGACCATTGTATGTATTGTCAGGTCTTTATATAAGAGTGAAACCTTTATTTATG | ||
| + | CTTCTTGTGAGTAGAGAATAAATTTTAAAACTAATTAGATGAAATTAAGAACAGAGTATTGGAACATCTC | ||
| + | TGTGTTTTCCGGAATGTTTACTCAGGTCCATGAATGCTGTGATGCTGGGAACTTAGGGAAGATTCACCAA | ||
| + | AATTTGAGAGTGATAAAAATGGCCTAAAATGGGAAATGGTGAGCACTCTTATTATTAGTGAGATTGGTCA | ||
| + | ATCAGCCCATTTGAAATGGGTAAAAAATACTTTCAGTAAAATAATTACATATTATATATAAAATATTTCT | ||
| + | TTTTTTGAGGGCAGCTACTGAGTGATAAAAGCATAGTAGAAATCACTTTGGTTAAAAGTAACTCAAATTT | ||
| + | TTCTCTTAAGTAATCTACCAACTTCCTGATGTTTTTCTTTAATATTTTGGAACTGTTCAAGACAGAATGG | ||
| + | AGCCTACATATATGGTGTTCCACTAGTATGTTGAAAATGTCATATCATGGAGAATGGAGACACCTTCCAG | ||
| + | GTGTCTGTTAAACCCATCTTCTCTGTGTACTTCTGGCATCTTTTTTGGTAGGATCATTTGGCAGGGGGTA | ||
| + | GAGTACCTGTACTTTTGGCACCATTGAAACCAGCTCTGGCCCACTTGTTTGAATAGCTATCAGACTCAGC | ||
| + | GTCTCTATATGCTTTATATACAGTTGAGAGAACCAGTGTTTAATGCTCTTGAAATTGTATCTTCCATGCT | ||
| + | TCAGACCAGATGCATTTAACAAAATGGATATATTCCAGCTGTAGTTGCCCAGTGTTTACTTAACACATCT | ||
| + | ACATTTTTTTCTTGTCTATTTTGGTCCCCTTGATAGGAAAAGCTATAATTTTAGGCAGGACTATACGTCG | ||
| + | ATTTGTAGCCATGCTTCCTTCCTTTCCCTTGCTCATCCATGTTAGCTGGCAGTTTTTCTTTTGAAAAGTT | ||
| + | AAAACCGGGATATGTGCAATAGAAATATATATATATATATGTATATTTTAATACTTGTGGACAAATGTTA | ||
| + | CAAGTTGTTTAAGAACAACAAAATCACCAATGTCTTCCATTTTGAGATGTGTATAGTTTTGTAAGCATTA | ||
| + | GTGCTTGGTAGCATATTGTAGTGCCATGTTAGGGGTTAGTGCATGAGTCTAGTGATTTTAAACTTCAGGA | ||
| + | TGAATTATTGATAATAACAAATAGTGTAAAAAGAGTGGAAAATCTAAACCTTTTCTTTTTCCATAATGTC | ||
| + | TAAATCTGTTATATTCTTCCTGGGGAAAAGAGATTAAGGCCCAAAAGACTCATTTATGAATAGAAATGTG | ||
| + | GGGTCAAAATCTGAGATACTATATTTGAGGACATTTCAGCTTTCCATAAGGTATCTGGAAACCAGCTGTC | ||
| + | TTTGTGTCTTATGAAACCTCAAGTCAAAATGAGACCGCATTTAATTATTCTGCTTTGCTCTTTTTTTGGG | ||
| + | GTGGGGGGGAGATAGAGTTTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTTTGCTCACTGTA | ||
| + | ACCTCTGCCTCTTGGGTTCAAGTGATTCTCGTGCCCCAGCCTCCCGAGTAGCTGGGACTACAGGCACGTG | ||
| + | CCACCACGCCCAGCTAATTTTTATATTTTTAGTAGAGACGGGGTTTTGCTGTGTTGGCCAGGCTGGTCTC | ||
| + | AAACTCCTGACCTCAAGTAATCCACCTGGCCTGCTCTTTTCATGTCTTAACATGGCATGTCTTTTAGTTT | ||
| + | CATTATTTTCCTACTCCTTGTATGTCAAGAAATTACATTTTGCATGTCTTATGGAGATGCTGTTAATTGC | ||
| + | TTCAGTGAGTGCTTTTCTAATCTGCAGACCATTTACATTTCCTGTTTGCAGCATGCTGTGTGCAAACACT | ||
| + | CAGTAATTTGGAGTATTCAATTATTTGTTAGGGCTCTTCCTATTTCCAAATGTGCTGAATTGTCTATTGA | ||
| + | TGGGATTTTCAGATCTTTTCATGAGAACTGGAAATGTAGCTGGGTGGCACCTACCTAGGTTGCTACGTAG | ||
| + | TGAGTAGACTTTCTCTTGGGTATAGTAAGCCTCAGACAGCTTTCACTTTTATCTACTTTACTTGTGGAAA | ||
| + | TAAAACAGTCATTTTGTTCTGAAAGAATAAGATAGCTTTCTGTAGAGAAGGAATTCCTACCTCTAAAAGC | ||
| + | TGCCTTGAGAACTCAGAACTGGCAGTTTTCTGAGGTGATTTTTAAATTTCAGTATTAGGGAGAGTCCAGC | ||
| + | ATTTGCTGACACAGATTCTACATAACTAATGTATGATAGCAAATGCAAAACTATTATAATGTGGTGTATC | ||
| + | TTGCGCATACACAGGTTAGAACAAGTAGACTCTGGCAGCAGATCTCCAGAGACCCAAGTTTAGGTTCTCA | ||
| + | TAGTGTATTTGAAGTAGTTATACTCCTGGCTTAAGTAGTTTAGTGCCTGGGAGAATCCATTACTGAAAAG | ||
| + | CATTTAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGAAAAGGTAGTGAATACAGAATAGCACAA | ||
| + | TATATAGGAAAGGATATTTCCTGTTGAAAAAAAAAGCCATAAAATTAATAGTAATTTTCAAGAGGTAGAA | ||
| + | TTTTAGTAGCAATAAGTTTGTGCATGTATAGTAATTTGCATTAGCAAGGTTGTACAATAGAATAAGTGAT | ||
| + | ACTACTGGAGTTTGTGGGGCTCTGCAACCAAGAGTTGCACAAGGGCCAGGTGCGGTGGCTTACACCTATA | ||
| + | ATCCCAGCACTTTGGGAGGCCAAGGTGGGCGGATCACAAGGTCAAGAGATCAGGACCATCCTGGCCAACA | ||
| + | TGGTGAAATGCCATCTCTACTAAAAATACAAAACTTAGCTGGGCATGGTGGCACATGCCTGTAATCCCAG | ||
| + | CTACTCAGGAGGCTGAGGCAGTAGAATCACTTAAACCTGAGAGGCGGAGGTTGCAGTGAGCTGAGATCAC | ||
| + | ACCACTGTACTCCAGCCTGGTGACAGTAAGACTCCACCTCAAACAAACAAAAAATGCACAAAAGGAAAAA | ||
| + | ATGTTCTGGATAAAGAGCATATGAAAGGTACTTTCTTTTAGAACAGTATGAATGTATATGAATTTATCTT | ||
| + | TATGTGAGAAATAATCTTTTTTGTAAGTTAAAGGTTAAAGACCCTGCTTGTTTCCTAAATCAGAGGGTTA | ||
| + | GAGCTGTTGAAATTAAACCTTTATAGTGCTTAACCTGGAAACAAATGTACTACAGCCCCACTGAAGCCGC | ||
| + | TCTATGGGTTTTTTGCTTACGATGTTTTAGCAACAGGCATTTATTTTGTAGAATGGTATACTTTCTGCTT | ||
| + | TGTCTCCTCATCTTTCTCCACTGGTTTCCTGGGTACATTTTTTTTAAAGGGAGAGAAGTTTGTGAGTTTA | ||
| + | ATGTTTTAAAAAAACTCCCAAGAACATGGATAAGCCGGATTTCTCTCATGCTTATGATTAGGGAGTTAGG | ||
| + | ATTTAAAGATGCAAAGCAGAAGGACTGAAAGGAATAGCCAGTGAATATGTTTCAGTGGGTGAGGTGTGAA | ||
| + | ACCTTGTCTAATATAGATGTGTCCTATGGCCTGTGACTGCTTATTCTTTATCAATGAGAACTCAGCAAAC | ||
| + | TAGTTTCTTTCTTGATCTGAGGAACCACACAGCTCACATCAGAATATAATAGTGGAACTAGGGTGACTTC | ||
| + | ACTCCCTTTCACCTGATGTCTATCTTGGCCTTTTAGCACCTTGACTATCCCTGAAAAGACTGGGTCTTTG | ||
| + | TTTTCCCAGGGAAAAAACGAAGTGATGTAGATCTAAGAAATAGTGCCTCTTTAATATTTAATTCTTACAT | ||
| + | GACAACTAACACACAGGGAAAAGGCTATGTTAAGTTGATTATAGCTCCTCTTAAAATGTCCTATGCTGTC | ||
| + | AGTTGGTCTTAGAGACATGGGAATAACCAAGCAATATTCAGACATCTGCACTATCTGGGGACAGCGCTAC | ||
| + | ATAGTACAATGATGTCAGCAGTTCAGTTACTTGGAACAATTAAAGATTACCCTTAACAGCCATGTTAATA | ||
| + | CATCTAAATGCTAAAATATACTACGAGTTTTTCATAGTCTTGAAAGTATACAGTTAATTACTTTTCAAAG | ||
| + | TTACTGTGGTCTCATGTTTACTCTTCTTGTATCTGTGATATGCAAAAAGATGAAGACTCTTGGCCTCCAG | ||
| + | GAGTTTACATTCTCATGGTGCTGTTGGTTCAAGCAGATTGCTTTAGTTTATTAATGAACATTGCTTGGCT | ||
| + | ATTAATTATATCCTATTTGGAAGGATCCCTTGGTGAACAGTTTTAAAAAGCAGAGGGCTGTGCTAAATTT | ||
| + | CAGGGTATTGATAGCTTGAGTTTACATTGTATTAGCCCTGCTCGTTATCATTTTTTTCCCCAGGGAGCTA | ||
| + | TGCAGGTAATGCTCATTAGCATGAATCAGAAAAGAAACCATTCTGCCTAAGAGCATCTTAACCATCCCCC | ||
| + | TAAACCACCTATGCTCTCCTGTTATAGTTGTCAGTAAATCACGAAGAAAATTAACAGCTCCTTAAGACTC | ||
| + | TACATCCCTCAATTCTCTTTCTTTTCCCAGAGTTTGTACATCATTCTCTACTCAGATGGACCAGGATTTG | ||
| + | CATATAAGCCTTTTAAAACTGACTAGTGGTCCTAATTTTAACATTTTTGTGTATGACTCCTATCCCTTTT | ||
| + | CTGAAGCAGCATTGTGATAGATGTGGAGCTTCTACCTGCATTTCTAAGCAATTATTAACCCAACTTTTGA | ||
| + | GTTGAAACTTGCATGGATCCTTGTTATGTCCCGTAATTCCTCTGAATCAAGGAATTTATTTTGCATGCTT | ||
| + | ATTAAACTCAAACTGGGTCTGATTGAAAGTGCAAGCAATAGTGACAGGACCTTAACTCTGGTTTTAGAGT | ||
| + | ACGAACATTCTAATGCATGACTCAAAATGTCCAGTGTTGTGGTTACTAGTTATTTTATATATGCATTTGT | ||
| + | TAATAATGAAGCAATAGAGACTAAGACCAATAGCTTGAATATATTCAGATTATTTTCATGTTTTAATAAA | ||
| + | GGATTTTTCTTACTTCCATTGAAAATGGGAAGTAGAAGATAAACAGGGGCAACCACAAGACATTCAAACT | ||
| + | GTCAGGACTAGGGCGTAGCAGTGCCCACCCTCTAACCACTTGCCTGGTTCCATTAGTCCCCAAGCATGTT | ||
| + | TGAAAACAAAAGACACAAACTAAGCATGCTGAATAAAACTAGCTGCTATGGCTGTAATAATCAGAAAGGT | ||
| + | GCAGAGTTTCAGAAAGAGTAGGAAAGTAGGGAATGCCAGTATGGAGTATGTAAAATAACTAGATATGGAT | ||
| + | ATGGGGTTTGTTTATGATATGCTTTATGTAGACAAAGTTTGAGGTCTTTTAAAACTGGGATTTGAGTTGA | ||
| + | AAAGAGTCAGGATGCACATATTTCCACTATTTCAGGATTTTCAGATGACTAGGCACATGTTGTGGATGAT | ||
| + | ACTGGTACCAACTACCAATGACCAACTATAAAGTACCCCTGCAATAAGGGAGACTTCCTTTTTGTAGAAT | ||
| + | AGTCCTGAAAATACTGACAGATCTGTGGTTAGTTTGTTATTTTATTGCATAAAAAACAGTTTAAACAAAT | ||
| + | TTTATAGCCAAAGTTTTATCCTTGATGGGTTTGGCCAGACTGCAATTTCTTGACTAAAGCTTTTAATGCC | ||
| + | AGGTTAAACAGGAGAAACTTTTTCCACTAGAAGAAAATCCTTGCTATCTATTTTTTCCAATAGAAGAAAA | ||
| + | TCCTTGCTATTTATTTTATTTGATGAATAAACAAATTTATTGCAGTAGCTTAAAAAAATTTTTTTTTTAA | ||
| + | ACAGTCTCACTCTGTCGCCCAGGCTGGAGTGAAGCAATGTGATCTCAGCTCACTGCAACCTCCACCTCCC | ||
| + | GAGTAGCTGGGATTACAGACATGCACCACCACCCTCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTT | ||
| + | TCGCCATGTTGGCCAGGCTGGTCTTTAACTCCTGGCCTTACGTGATCCGCCCCCCCTTAGCCTTCCAAAG | ||
| + | TGCTGGGATTACAGGTGTGAGCCACTGCACCTGGCCTGTAGTAGCTTAAAATTTTCCTTGAGAAAATTCC | ||
| + | TGACTTTAAAAATAACCCTTATATAAGTACAAGTGATTGTGACAAATGACGTAAAAATGGCATTCATGAT | ||
| + | GTCTGAAACAAGCCTAAATAGAATTCAAGATTAGACTAAATGATTTTCACAAAGCACATTCAAGGTTTTA | ||
| + | CATTCTATGATTGAAAAAAATTTTTTGAAAACTTTTTATTTCATTCTTTCCTGTAGGATTTTGCTACAAA | ||
| + | TAACTTTGGGAATGAATAAAGTGGAATGGTAACTTTCCAGTGGTTCAGAATTGAATTAGACTTCTTGTGA | ||
| + | CTGTGATGTTTGGTTTCCATTGAAATATATGAAGTGAGATGTCATATCCTGAATATAGTTTGTCTTCCCC | ||
| + | AATTACTTGATAGCATGTCTGTCAGCCAGTAAAGATTAAGAACAGAGTTTCTCTAAATTCCTCCGATTAT | ||
| + | TCCACTAAGGCACATTAAAATACTTAATTTTGGGAAACCAGACATCACAGATTTCTCCATGAAGTCCTAA | ||
| + | ATCTTCTTTAAAGTCAGAATAGGTATCTTAGTTACTGACAGTATTCAGGTTTTTTTCTCCCTTGGTGATA | ||
| + | TGTCATTCCATCAGTGAAAAAATATTTTCTCCCAGGGATAAGAAAGGTATTCTGGTAATACATTATCATC | ||
| + | AATCCTTAAACAGTAACAGTCTTGGCACTTATCACAAAACCGACCCATTTCTTATAACCAGAAAGATTAT | ||
| + | CTTAGACTGTCCTTCACATTATACTTTACCTACTGCCTTGTAAGAATAAGAGTTGCTCACTGTGTTTACT | ||
| + | TGCTGTCCTCCATATTCTCCATTGCACCATTGGTGTATAACGTTAAGAGTTTCATTGAATATTATTTTAA | ||
| + | GTATTACAAAAGGCAGCTTGCTTCTTAATCTATGCATCTTTGGGGTTTTTGAAGAAATTTAATTCTTTGA | ||
| + | TGTAAAAAGGAACTGTTAAAAAAGTTGGAAGCTCTGCACCTGTGTATATATATATTTTAGCAATAAAGCA | ||
| + | GCATGGGCTGAGAATGCACTGAAA</dnaseq> | ||
Latest revision as of 05:12, 17 November 2018
CYRANO- long noncoding RNA required for proper embryonic development
Contents
Annotated Information
Approved Symbol
CYRANO
Approved Name
CYRANO. linc-oip5 [1]. 1700020I14Rik [1].
Characteristics
8.2-kb CYRANO molecule is a long intergenic ncRNA (lincRNA) transcribed in mouse ESCs [1][2][3][4]. CYRANO shares unique sequence complementarity with the differentiation-associated microRNA [4]. CYRANO distribution in the cell could indicate that nuclear and cytoplasmic CYRANO pools that have distinct functions or that it interacts with proteins that shuttle from the nucleus to the cytoplasm [4]. CYRANO posseses an almost complete binding site to mir-7 in mouse ESCs and this binding site is conserved across vertebrates [5].
Function
lincRNA CYRANO is required for proper embryonic development [3]. Depletion of CYRANO results in disarray in the pluripotent gene expression signature and defects in self-renewal maintenance [4]. CYRANO is one factor that presents a barrier to lineage commitment in conditions of suboptimal fluctuations in Nanog levels [4]. CYRANO function as a sponge of RBPs from mRNAs [6]. CYRANO in various systems include anti-proliferative functions and play roles in organogenesis/embryonic development [4].
Regulation
mir-7 regulates CYRANO [3].
Disease
Expression
CYRANO is expressed in zebrafish nervous system. Brain and notochord at 24 hours post fertilization (hpf), expression at 72 hpf also includes zebrafish spinal cord. Mouse and human homolgoues are widely expressed in adult and developing tissues and cell lines including the brain. RNA stability half-life of mouse homologue was 3-4 hr in neuroblastoma cells, close to lncRNA median [10]
Labs working on this lncRNA
- Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA
- Department of General Surgery, the First Hospital of Jilin University, Changchun, 130021, China
- Department of General Surgery, the First Hospital of Jilin University, Changchun, 130021, China
- Laboratory of Genetics and Genomics, National Institute on Aging-Intramural Research Program, NIH, Baltimore, MD 21224, USA
References
- ↑ 1.0 1.1 1.2 Chew G.L., Pauli A., Rinn J.L., Regev A., Schier A.F., Valen E. Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs. Development. 2013;140:2828–2834
- ↑ Guttman M., Garber M., Levin J.Z., Donaghey J., Robinson J., Adiconis X., Fan L., Koziol M.J., Gnirke A., Nusbaum C. Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs. Nat. Biotechnol. 2010;28:503–510
- ↑ 3.0 3.1 3.2 Ulitsky I., Shkumatava A., Jan C.H., Sive H., Bartel D.P. Conserved function of lincRNAs in vertebrate embryonic development despite rapid sequence evolution. Cell. 2011;147:1537–1550.
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Smith KN, Starmer J, Miller SC, Sethupathy P & Magnuson T. Long Noncoding RNA Moderates MicroRNA Activity to Maintain Self-Renewal in Embryonic Stem Cells[J]. Stem Cell Rep. 2017, 9(1):108-121.
- ↑ Sigova A.A., Mullen A.C., Molinie B., Gupta S., Orlando D.A., Guenther M.G., Almada A.E., Lin C., Sharp P.A., Giallourakis C.C. Divergent transcription of noncoding RNA/mRNA gene pairs in embryonic stem cells. Proc. Natl. Acad. Sci. USA. 2013;110:2876–2881.
- ↑ Kim J., Abdelmohsen K., Yang X., De S., Grammatikakis I., Noh J.H., Gorospe M. LncRNA OIP5-AS1/cyrano sponges RNA-binding protein HuR. Nucleic Acids Res. 2016;44:2378–2392
- ↑ Zhi F, Wang Q, Xue L, Shao NY, Wang R, Deng DN et al. The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma[J]. Plos One. 2015, 10(8).
- ↑ Hu GW, Wu L, Kuang W, Chen Y, Zhu XG, Guo H et al. Knockdown of linc-OIP5 inhibits proliferation and migration of glioma cells through down-regulation of YAP-NOTCH signaling pathway[J]. Gene. 2017, 610(24-31
- ↑ Zhang Z, Liu F, Yang F & Liu YH. Kockdown of OIP5-AS1 expression inhibits proliferation, metastasis and EMT progress in hepatoblastoma cells through up-regulating miR-186a-5p and down-regulating ZEB1[J]. Biomed Pharmacother. 2018.
- ↑ Clark MB, Johnston RL, Inostroza-Ponta M, Fox AH, Fortini E, Moscato P et al. Genome-wide analysis of long noncoding RNA stability[J]. Genome Res. 2012, 22(5):885-898.
Sequence
>gi|729082|ref|NR_026757.2|Homo sapiens OIP5 antisense RNA 1 (OIP5-AS1), transcript variant 1, long non-coding RNA
000001 AGGGTTGGCC ACCTTGAGAA GCTGCGAAGA TGGCGGAGTA AGGCGTGCCG CTGCAAACTG GCCTCTGGGC CGGGGGCGAG 000080
000081 CAGCCCCCGG GAGGCCGAGT GCATCTGTTG GACCGTGCGA GGAGAAGAAA AAAAATATCG GCCAGAGGAG AGGAACTAAC 000160
000161 CGAACATTCT TCCTCCCTAC CTTATAGAGG GGAGTGGTCA TCTACACTAA AGCAAAGTGT TAGGCGCGTC CGTGCGAAGA 000240
000241 GACCACCAAA CAGGCTTTGT GTTCCTTATC ACAGGAAGAA AATTTTCCTT GACCTTTAGG TGCTTTTATA TTCATCTCAA 000320
000321 AAACAAAATT CTGAACTCAG GACTTGGCAA GTGTCTCTAT GTTGTCTCCT AGAGTGGGTA GTCCTGCTTC TTTTACCCAG 000400
000401 TTACTTTCCC GTTTTTGAAA TGTGGCTATC ACTTCTCTAC ACATTACCTC CATGATTTGG AATGGAAAAG GCCACTTTTC 000480
000481 TTTTTGTTCT GCCTCTCAAA TTCAACACAG AGGAGCTCCT AGGATTCCAG TTATCCTGCT AACATCTCCA GGAAGAAAGA 000560
000561 AGCAACTCAC ATGGGTCTTT TGCTGTTTGC TTAATTATAA AGACATCATT TTGCAAGCAG AAGGCTGAGT TTCATTTGAA 000640
000641 ACAGGTGCTT AGGTGGTGGT ATTTGTGAAT ACTTTTCATT CCAAGCAAGA AGACTAAAGA AGTAGCAAGT ATGAATGACT 000720
000721 TCAGGGTTTA AAAAAAATGT CTTCCAGTTT CAGCCACTAC CATGATAAGC ACAGTTGAGA CTGCAGCAGT AAATTCCAAA 000800
000801 TATGTGTTTC TAATTTGACG TGAAAGATAC TAAAAATTTA TATTTGTATA TTTAAATCCT GGCTCATCCT GTGACATAGA 000880
000881 TTTACTGAAT AGGAACAAAG GCCCAATTTT AAACAAAAAC CTAGGCCGGG TGTGGTGGCT CACACCTGTA ATCCCAACAC 000960
000961 TTTGGGAGGC CAAGGCAGGC AAATCACCTG AGGTTGGGAG TTTGAGACCA GCCTGACCAA CATGGAGAAA CCCCATCTCT 001040
001041 ACTAAAAATA GAAAATTAGC CAGGTGTGGT GGTAGATACG TGTAATCCCA GCTACTCGTG AGGCTGAGGC AGGAGAATTG 001120
001121 CTTGAATCCG GGAGGCGGAG GTTGCAGTGA ACTGAGATTG CACCACTGCA CTCCAGCCTG GGCGACAGAG TGAGACTCCG 001200
001201 TCTCAGAAAA ATAAAAAACA ACTTTGGACA TAGAGGAGTT GGGGGCAGAG GGGGGAGGAT GAGAGAATTT TCCATGTAAC 001280
001281 TCCTTTTCCT TAAGAAAAAG AAGCAAAAAT CCCTCATGCA GTGCCATCTG ACTTTATGGT GTTTCACATT ATATAGTTAC 001360
001361 TTTTTTTAAT AAACGCATAA GATTAACTCT TCCTGCAACC CAAGGTGGAT ACTTGGATGT TTGATTTTTA TTTATTTATT 001440
001441 TATTTATTTT TTGAGGCAGT CTCTCTCTGT TGCCCAGGCT GGAGTGATGC GTGATTTCTG CTCACTGCAG TCTCTCCCAC 001520
001521 CCGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCAAGTAG CTAGGATTAC AGGCGCGCAC CACCACGCTC AGCCTGATTT 001600
001601 CTAGGCTGAA AAGGCTGGGA CTCAGGTACT TTCCCCAGTT GGGACTGAAC TTTCTCATCA GAGAATGGGC CTCAGAAGCA 001680
001681 ATGTTCCCAA ACTTGTGGTT GGGTCATCGT GGAAAAGAAA CCTCAAATAA GAAAAATAAT TACAATAAGA AATGGATTTT 001760
001761 CTTTTTTCCC ACAACAGTTA TACATTATAA AGAACAGACT GTCGTAGAAA ACTGTCTTTG CTTCCAAATC AGCAGAGGAC 001840
001841 CATTGTATGT ATTGTCAGGT CTTTATATAA GAGTGAAACC TTTATTTATG CTTCTTGTGA GTAGAGAATA AATTTTAAAA 001920
001921 CTAATTAGAT GAAATTAAGA ACAGAGTATT GGAACATCTC TGTGTTTTCC GGAATGTTTA CTCAGGTCCA TGAATGCTGT 002000
002001 GATGCTGGGA ACTTAGGGAA GATTCACCAA AATTTGAGAG TGATAAAAAT GGCCTAAAAT GGGAAATGGT GAGCACTCTT 002080
002081 ATTATTAGTG AGATTGGTCA ATCAGCCCAT TTGAAATGGG TAAAAAATAC TTTCAGTAAA ATAATTACAT ATTATATATA 002160
002161 AAATATTTCT TTTTTTGAGG GCAGCTACTG AGTGATAAAA GCATAGTAGA AATCACTTTG GTTAAAAGTA ACTCAAATTT 002240
002241 TTCTCTTAAG TAATCTACCA ACTTCCTGAT GTTTTTCTTT AATATTTTGG AACTGTTCAA GACAGAATGG AGCCTACATA 002320
002321 TATGGTGTTC CACTAGTATG TTGAAAATGT CATATCATGG AGAATGGAGA CACCTTCCAG GTGTCTGTTA AACCCATCTT 002400
002401 CTCTGTGTAC TTCTGGCATC TTTTTTGGTA GGATCATTTG GCAGGGGGTA GAGTACCTGT ACTTTTGGCA CCATTGAAAC 002480
002481 CAGCTCTGGC CCACTTGTTT GAATAGCTAT CAGACTCAGC GTCTCTATAT GCTTTATATA CAGTTGAGAG AACCAGTGTT 002560
002561 TAATGCTCTT GAAATTGTAT CTTCCATGCT TCAGACCAGA TGCATTTAAC AAAATGGATA TATTCCAGCT GTAGTTGCCC 002640
002641 AGTGTTTACT TAACACATCT ACATTTTTTT CTTGTCTATT TTGGTCCCCT TGATAGGAAA AGCTATAATT TTAGGCAGGA 002720
002721 CTATACGTCG ATTTGTAGCC ATGCTTCCTT CCTTTCCCTT GCTCATCCAT GTTAGCTGGC AGTTTTTCTT TTGAAAAGTT 002800
002801 AAAACCGGGA TATGTGCAAT AGAAATATAT ATATATATAT GTATATTTTA ATACTTGTGG ACAAATGTTA CAAGTTGTTT 002880
002881 AAGAACAACA AAATCACCAA TGTCTTCCAT TTTGAGATGT GTATAGTTTT GTAAGCATTA GTGCTTGGTA GCATATTGTA 002960
002961 GTGCCATGTT AGGGGTTAGT GCATGAGTCT AGTGATTTTA AACTTCAGGA TGAATTATTG ATAATAACAA ATAGTGTAAA 003040
003041 AAGAGTGGAA AATCTAAACC TTTTCTTTTT CCATAATGTC TAAATCTGTT ATATTCTTCC TGGGGAAAAG AGATTAAGGC 003120
003121 CCAAAAGACT CATTTATGAA TAGAAATGTG GGGTCAAAAT CTGAGATACT ATATTTGAGG ACATTTCAGC TTTCCATAAG 003200
003201 GTATCTGGAA ACCAGCTGTC TTTGTGTCTT ATGAAACCTC AAGTCAAAAT GAGACCGCAT TTAATTATTC TGCTTTGCTC 003280
003281 TTTTTTTGGG GTGGGGGGGA GATAGAGTTT CACTCTGTCA CCCAGGCTGG AGTGCAGTGG CACAATCTTT GCTCACTGTA 003360
003361 ACCTCTGCCT CTTGGGTTCA AGTGATTCTC GTGCCCCAGC CTCCCGAGTA GCTGGGACTA CAGGCACGTG CCACCACGCC 003440
003441 CAGCTAATTT TTATATTTTT AGTAGAGACG GGGTTTTGCT GTGTTGGCCA GGCTGGTCTC AAACTCCTGA CCTCAAGTAA 003520
003521 TCCACCTGGC CTGCTCTTTT CATGTCTTAA CATGGCATGT CTTTTAGTTT CATTATTTTC CTACTCCTTG TATGTCAAGA 003600
003601 AATTACATTT TGCATGTCTT ATGGAGATGC TGTTAATTGC TTCAGTGAGT GCTTTTCTAA TCTGCAGACC ATTTACATTT 003680
003681 CCTGTTTGCA GCATGCTGTG TGCAAACACT CAGTAATTTG GAGTATTCAA TTATTTGTTA GGGCTCTTCC TATTTCCAAA 003760
003761 TGTGCTGAAT TGTCTATTGA TGGGATTTTC AGATCTTTTC ATGAGAACTG GAAATGTAGC TGGGTGGCAC CTACCTAGGT 003840
003841 TGCTACGTAG TGAGTAGACT TTCTCTTGGG TATAGTAAGC CTCAGACAGC TTTCACTTTT ATCTACTTTA CTTGTGGAAA 003920
003921 TAAAACAGTC ATTTTGTTCT GAAAGAATAA GATAGCTTTC TGTAGAGAAG GAATTCCTAC CTCTAAAAGC TGCCTTGAGA 004000
004001 ACTCAGAACT GGCAGTTTTC TGAGGTGATT TTTAAATTTC AGTATTAGGG AGAGTCCAGC ATTTGCTGAC ACAGATTCTA 004080
004081 CATAACTAAT GTATGATAGC AAATGCAAAA CTATTATAAT GTGGTGTATC TTGCGCATAC ACAGGTTAGA ACAAGTAGAC 004160
004161 TCTGGCAGCA GATCTCCAGA GACCCAAGTT TAGGTTCTCA TAGTGTATTT GAAGTAGTTA TACTCCTGGC TTAAGTAGTT 004240
004241 TAGTGCCTGG GAGAATCCAT TACTGAAAAG CATTTAACTT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAC TGAAAAGGTA 004320
004321 GTGAATACAG AATAGCACAA TATATAGGAA AGGATATTTC CTGTTGAAAA AAAAAGCCAT AAAATTAATA GTAATTTTCA 004400
004401 AGAGGTAGAA TTTTAGTAGC AATAAGTTTG TGCATGTATA GTAATTTGCA TTAGCAAGGT TGTACAATAG AATAAGTGAT 004480
004481 ACTACTGGAG TTTGTGGGGC TCTGCAACCA AGAGTTGCAC AAGGGCCAGG TGCGGTGGCT TACACCTATA ATCCCAGCAC 004560
004561 TTTGGGAGGC CAAGGTGGGC GGATCACAAG GTCAAGAGAT CAGGACCATC CTGGCCAACA TGGTGAAATG CCATCTCTAC 004640
004641 TAAAAATACA AAACTTAGCT GGGCATGGTG GCACATGCCT GTAATCCCAG CTACTCAGGA GGCTGAGGCA GTAGAATCAC 004720
004721 TTAAACCTGA GAGGCGGAGG TTGCAGTGAG CTGAGATCAC ACCACTGTAC TCCAGCCTGG TGACAGTAAG ACTCCACCTC 004800
004801 AAACAAACAA AAAATGCACA AAAGGAAAAA ATGTTCTGGA TAAAGAGCAT ATGAAAGGTA CTTTCTTTTA GAACAGTATG 004880
004881 AATGTATATG AATTTATCTT TATGTGAGAA ATAATCTTTT TTGTAAGTTA AAGGTTAAAG ACCCTGCTTG TTTCCTAAAT 004960
004961 CAGAGGGTTA GAGCTGTTGA AATTAAACCT TTATAGTGCT TAACCTGGAA ACAAATGTAC TACAGCCCCA CTGAAGCCGC 005040
005041 TCTATGGGTT TTTTGCTTAC GATGTTTTAG CAACAGGCAT TTATTTTGTA GAATGGTATA CTTTCTGCTT TGTCTCCTCA 005120
005121 TCTTTCTCCA CTGGTTTCCT GGGTACATTT TTTTTAAAGG GAGAGAAGTT TGTGAGTTTA ATGTTTTAAA AAAACTCCCA 005200
005201 AGAACATGGA TAAGCCGGAT TTCTCTCATG CTTATGATTA GGGAGTTAGG ATTTAAAGAT GCAAAGCAGA AGGACTGAAA 005280
005281 GGAATAGCCA GTGAATATGT TTCAGTGGGT GAGGTGTGAA ACCTTGTCTA ATATAGATGT GTCCTATGGC CTGTGACTGC 005360
005361 TTATTCTTTA TCAATGAGAA CTCAGCAAAC TAGTTTCTTT CTTGATCTGA GGAACCACAC AGCTCACATC AGAATATAAT 005440
005441 AGTGGAACTA GGGTGACTTC ACTCCCTTTC ACCTGATGTC TATCTTGGCC TTTTAGCACC TTGACTATCC CTGAAAAGAC 005520
005521 TGGGTCTTTG TTTTCCCAGG GAAAAAACGA AGTGATGTAG ATCTAAGAAA TAGTGCCTCT TTAATATTTA ATTCTTACAT 005600
005601 GACAACTAAC ACACAGGGAA AAGGCTATGT TAAGTTGATT ATAGCTCCTC TTAAAATGTC CTATGCTGTC AGTTGGTCTT 005680
005681 AGAGACATGG GAATAACCAA GCAATATTCA GACATCTGCA CTATCTGGGG ACAGCGCTAC ATAGTACAAT GATGTCAGCA 005760
005761 GTTCAGTTAC TTGGAACAAT TAAAGATTAC CCTTAACAGC CATGTTAATA CATCTAAATG CTAAAATATA CTACGAGTTT 005840
005841 TTCATAGTCT TGAAAGTATA CAGTTAATTA CTTTTCAAAG TTACTGTGGT CTCATGTTTA CTCTTCTTGT ATCTGTGATA 005920
005921 TGCAAAAAGA TGAAGACTCT TGGCCTCCAG GAGTTTACAT TCTCATGGTG CTGTTGGTTC AAGCAGATTG CTTTAGTTTA 006000
006001 TTAATGAACA TTGCTTGGCT ATTAATTATA TCCTATTTGG AAGGATCCCT TGGTGAACAG TTTTAAAAAG CAGAGGGCTG 006080
006081 TGCTAAATTT CAGGGTATTG ATAGCTTGAG TTTACATTGT ATTAGCCCTG CTCGTTATCA TTTTTTTCCC CAGGGAGCTA 006160
006161 TGCAGGTAAT GCTCATTAGC ATGAATCAGA AAAGAAACCA TTCTGCCTAA GAGCATCTTA ACCATCCCCC TAAACCACCT 006240
006241 ATGCTCTCCT GTTATAGTTG TCAGTAAATC ACGAAGAAAA TTAACAGCTC CTTAAGACTC TACATCCCTC AATTCTCTTT 006320
006321 CTTTTCCCAG AGTTTGTACA TCATTCTCTA CTCAGATGGA CCAGGATTTG CATATAAGCC TTTTAAAACT GACTAGTGGT 006400
006401 CCTAATTTTA ACATTTTTGT GTATGACTCC TATCCCTTTT CTGAAGCAGC ATTGTGATAG ATGTGGAGCT TCTACCTGCA 006480
006481 TTTCTAAGCA ATTATTAACC CAACTTTTGA GTTGAAACTT GCATGGATCC TTGTTATGTC CCGTAATTCC TCTGAATCAA 006560
006561 GGAATTTATT TTGCATGCTT ATTAAACTCA AACTGGGTCT GATTGAAAGT GCAAGCAATA GTGACAGGAC CTTAACTCTG 006640
006641 GTTTTAGAGT ACGAACATTC TAATGCATGA CTCAAAATGT CCAGTGTTGT GGTTACTAGT TATTTTATAT ATGCATTTGT 006720
006721 TAATAATGAA GCAATAGAGA CTAAGACCAA TAGCTTGAAT ATATTCAGAT TATTTTCATG TTTTAATAAA GGATTTTTCT 006800
006801 TACTTCCATT GAAAATGGGA AGTAGAAGAT AAACAGGGGC AACCACAAGA CATTCAAACT GTCAGGACTA GGGCGTAGCA 006880
006881 GTGCCCACCC TCTAACCACT TGCCTGGTTC CATTAGTCCC CAAGCATGTT TGAAAACAAA AGACACAAAC TAAGCATGCT 006960
006961 GAATAAAACT AGCTGCTATG GCTGTAATAA TCAGAAAGGT GCAGAGTTTC AGAAAGAGTA GGAAAGTAGG GAATGCCAGT 007040
007041 ATGGAGTATG TAAAATAACT AGATATGGAT ATGGGGTTTG TTTATGATAT GCTTTATGTA GACAAAGTTT GAGGTCTTTT 007120
007121 AAAACTGGGA TTTGAGTTGA AAAGAGTCAG GATGCACATA TTTCCACTAT TTCAGGATTT TCAGATGACT AGGCACATGT 007200
007201 TGTGGATGAT ACTGGTACCA ACTACCAATG ACCAACTATA AAGTACCCCT GCAATAAGGG AGACTTCCTT TTTGTAGAAT 007280
007281 AGTCCTGAAA ATACTGACAG ATCTGTGGTT AGTTTGTTAT TTTATTGCAT AAAAAACAGT TTAAACAAAT TTTATAGCCA 007360
007361 AAGTTTTATC CTTGATGGGT TTGGCCAGAC TGCAATTTCT TGACTAAAGC TTTTAATGCC AGGTTAAACA GGAGAAACTT 007440
007441 TTTCCACTAG AAGAAAATCC TTGCTATCTA TTTTTTCCAA TAGAAGAAAA TCCTTGCTAT TTATTTTATT TGATGAATAA 007520
007521 ACAAATTTAT TGCAGTAGCT TAAAAAAATT TTTTTTTTAA ACAGTCTCAC TCTGTCGCCC AGGCTGGAGT GAAGCAATGT 007600
007601 GATCTCAGCT CACTGCAACC TCCACCTCCC GAGTAGCTGG GATTACAGAC ATGCACCACC ACCCTCAGCT AATTTTTGTA 007680
007681 TTTTTAGTAG AGACGGGGTT TCGCCATGTT GGCCAGGCTG GTCTTTAACT CCTGGCCTTA CGTGATCCGC CCCCCCTTAG 007760
007761 CCTTCCAAAG TGCTGGGATT ACAGGTGTGA GCCACTGCAC CTGGCCTGTA GTAGCTTAAA ATTTTCCTTG AGAAAATTCC 007840
007841 TGACTTTAAA AATAACCCTT ATATAAGTAC AAGTGATTGT GACAAATGAC GTAAAAATGG CATTCATGAT GTCTGAAACA 007920
007921 AGCCTAAATA GAATTCAAGA TTAGACTAAA TGATTTTCAC AAAGCACATT CAAGGTTTTA CATTCTATGA TTGAAAAAAA 008000
008001 TTTTTTGAAA ACTTTTTATT TCATTCTTTC CTGTAGGATT TTGCTACAAA TAACTTTGGG AATGAATAAA GTGGAATGGT 008080
008081 AACTTTCCAG TGGTTCAGAA TTGAATTAGA CTTCTTGTGA CTGTGATGTT TGGTTTCCAT TGAAATATAT GAAGTGAGAT 008160
008161 GTCATATCCT GAATATAGTT TGTCTTCCCC AATTACTTGA TAGCATGTCT GTCAGCCAGT AAAGATTAAG AACAGAGTTT 008240
008241 CTCTAAATTC CTCCGATTAT TCCACTAAGG CACATTAAAA TACTTAATTT TGGGAAACCA GACATCACAG ATTTCTCCAT 008320
008321 GAAGTCCTAA ATCTTCTTTA AAGTCAGAAT AGGTATCTTA GTTACTGACA GTATTCAGGT TTTTTTCTCC CTTGGTGATA 008400
008401 TGTCATTCCA TCAGTGAAAA AATATTTTCT CCCAGGGATA AGAAAGGTAT TCTGGTAATA CATTATCATC AATCCTTAAA 008480
008481 CAGTAACAGT CTTGGCACTT ATCACAAAAC CGACCCATTT CTTATAACCA GAAAGATTAT CTTAGACTGT CCTTCACATT 008560
008561 ATACTTTACC TACTGCCTTG TAAGAATAAG AGTTGCTCAC TGTGTTTACT TGCTGTCCTC CATATTCTCC ATTGCACCAT 008640
008641 TGGTGTATAA CGTTAAGAGT TTCATTGAAT ATTATTTTAA GTATTACAAA AGGCAGCTTG CTTCTTAATC TATGCATCTT 008720
008721 TGGGGTTTTT GAAGAAATTT AATTCTTTGA TGTAAAAAGG AACTGTTAAA AAAGTTGGAA GCTCTGCACC TGTGTATATA 008800
008801 TATATTTTAG CAATAAAGCA GCATGGGCTG AGAATGCACT GAAA
000081 CAGCCCCCGG GAGGCCGAGT GCATCTGTTG GACCGTGCGA GGAGAAGAAA AAAAATATCG GCCAGAGGAG AGGAACTAAC 000160
000161 CGAACATTCT TCCTCCCTAC CTTATAGAGG GGAGTGGTCA TCTACACTAA AGCAAAGTGT TAGGCGCGTC CGTGCGAAGA 000240
000241 GACCACCAAA CAGGCTTTGT GTTCCTTATC ACAGGAAGAA AATTTTCCTT GACCTTTAGG TGCTTTTATA TTCATCTCAA 000320
000321 AAACAAAATT CTGAACTCAG GACTTGGCAA GTGTCTCTAT GTTGTCTCCT AGAGTGGGTA GTCCTGCTTC TTTTACCCAG 000400
000401 TTACTTTCCC GTTTTTGAAA TGTGGCTATC ACTTCTCTAC ACATTACCTC CATGATTTGG AATGGAAAAG GCCACTTTTC 000480
000481 TTTTTGTTCT GCCTCTCAAA TTCAACACAG AGGAGCTCCT AGGATTCCAG TTATCCTGCT AACATCTCCA GGAAGAAAGA 000560
000561 AGCAACTCAC ATGGGTCTTT TGCTGTTTGC TTAATTATAA AGACATCATT TTGCAAGCAG AAGGCTGAGT TTCATTTGAA 000640
000641 ACAGGTGCTT AGGTGGTGGT ATTTGTGAAT ACTTTTCATT CCAAGCAAGA AGACTAAAGA AGTAGCAAGT ATGAATGACT 000720
000721 TCAGGGTTTA AAAAAAATGT CTTCCAGTTT CAGCCACTAC CATGATAAGC ACAGTTGAGA CTGCAGCAGT AAATTCCAAA 000800
000801 TATGTGTTTC TAATTTGACG TGAAAGATAC TAAAAATTTA TATTTGTATA TTTAAATCCT GGCTCATCCT GTGACATAGA 000880
000881 TTTACTGAAT AGGAACAAAG GCCCAATTTT AAACAAAAAC CTAGGCCGGG TGTGGTGGCT CACACCTGTA ATCCCAACAC 000960
000961 TTTGGGAGGC CAAGGCAGGC AAATCACCTG AGGTTGGGAG TTTGAGACCA GCCTGACCAA CATGGAGAAA CCCCATCTCT 001040
001041 ACTAAAAATA GAAAATTAGC CAGGTGTGGT GGTAGATACG TGTAATCCCA GCTACTCGTG AGGCTGAGGC AGGAGAATTG 001120
001121 CTTGAATCCG GGAGGCGGAG GTTGCAGTGA ACTGAGATTG CACCACTGCA CTCCAGCCTG GGCGACAGAG TGAGACTCCG 001200
001201 TCTCAGAAAA ATAAAAAACA ACTTTGGACA TAGAGGAGTT GGGGGCAGAG GGGGGAGGAT GAGAGAATTT TCCATGTAAC 001280
001281 TCCTTTTCCT TAAGAAAAAG AAGCAAAAAT CCCTCATGCA GTGCCATCTG ACTTTATGGT GTTTCACATT ATATAGTTAC 001360
001361 TTTTTTTAAT AAACGCATAA GATTAACTCT TCCTGCAACC CAAGGTGGAT ACTTGGATGT TTGATTTTTA TTTATTTATT 001440
001441 TATTTATTTT TTGAGGCAGT CTCTCTCTGT TGCCCAGGCT GGAGTGATGC GTGATTTCTG CTCACTGCAG TCTCTCCCAC 001520
001521 CCGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCAAGTAG CTAGGATTAC AGGCGCGCAC CACCACGCTC AGCCTGATTT 001600
001601 CTAGGCTGAA AAGGCTGGGA CTCAGGTACT TTCCCCAGTT GGGACTGAAC TTTCTCATCA GAGAATGGGC CTCAGAAGCA 001680
001681 ATGTTCCCAA ACTTGTGGTT GGGTCATCGT GGAAAAGAAA CCTCAAATAA GAAAAATAAT TACAATAAGA AATGGATTTT 001760
001761 CTTTTTTCCC ACAACAGTTA TACATTATAA AGAACAGACT GTCGTAGAAA ACTGTCTTTG CTTCCAAATC AGCAGAGGAC 001840
001841 CATTGTATGT ATTGTCAGGT CTTTATATAA GAGTGAAACC TTTATTTATG CTTCTTGTGA GTAGAGAATA AATTTTAAAA 001920
001921 CTAATTAGAT GAAATTAAGA ACAGAGTATT GGAACATCTC TGTGTTTTCC GGAATGTTTA CTCAGGTCCA TGAATGCTGT 002000
002001 GATGCTGGGA ACTTAGGGAA GATTCACCAA AATTTGAGAG TGATAAAAAT GGCCTAAAAT GGGAAATGGT GAGCACTCTT 002080
002081 ATTATTAGTG AGATTGGTCA ATCAGCCCAT TTGAAATGGG TAAAAAATAC TTTCAGTAAA ATAATTACAT ATTATATATA 002160
002161 AAATATTTCT TTTTTTGAGG GCAGCTACTG AGTGATAAAA GCATAGTAGA AATCACTTTG GTTAAAAGTA ACTCAAATTT 002240
002241 TTCTCTTAAG TAATCTACCA ACTTCCTGAT GTTTTTCTTT AATATTTTGG AACTGTTCAA GACAGAATGG AGCCTACATA 002320
002321 TATGGTGTTC CACTAGTATG TTGAAAATGT CATATCATGG AGAATGGAGA CACCTTCCAG GTGTCTGTTA AACCCATCTT 002400
002401 CTCTGTGTAC TTCTGGCATC TTTTTTGGTA GGATCATTTG GCAGGGGGTA GAGTACCTGT ACTTTTGGCA CCATTGAAAC 002480
002481 CAGCTCTGGC CCACTTGTTT GAATAGCTAT CAGACTCAGC GTCTCTATAT GCTTTATATA CAGTTGAGAG AACCAGTGTT 002560
002561 TAATGCTCTT GAAATTGTAT CTTCCATGCT TCAGACCAGA TGCATTTAAC AAAATGGATA TATTCCAGCT GTAGTTGCCC 002640
002641 AGTGTTTACT TAACACATCT ACATTTTTTT CTTGTCTATT TTGGTCCCCT TGATAGGAAA AGCTATAATT TTAGGCAGGA 002720
002721 CTATACGTCG ATTTGTAGCC ATGCTTCCTT CCTTTCCCTT GCTCATCCAT GTTAGCTGGC AGTTTTTCTT TTGAAAAGTT 002800
002801 AAAACCGGGA TATGTGCAAT AGAAATATAT ATATATATAT GTATATTTTA ATACTTGTGG ACAAATGTTA CAAGTTGTTT 002880
002881 AAGAACAACA AAATCACCAA TGTCTTCCAT TTTGAGATGT GTATAGTTTT GTAAGCATTA GTGCTTGGTA GCATATTGTA 002960
002961 GTGCCATGTT AGGGGTTAGT GCATGAGTCT AGTGATTTTA AACTTCAGGA TGAATTATTG ATAATAACAA ATAGTGTAAA 003040
003041 AAGAGTGGAA AATCTAAACC TTTTCTTTTT CCATAATGTC TAAATCTGTT ATATTCTTCC TGGGGAAAAG AGATTAAGGC 003120
003121 CCAAAAGACT CATTTATGAA TAGAAATGTG GGGTCAAAAT CTGAGATACT ATATTTGAGG ACATTTCAGC TTTCCATAAG 003200
003201 GTATCTGGAA ACCAGCTGTC TTTGTGTCTT ATGAAACCTC AAGTCAAAAT GAGACCGCAT TTAATTATTC TGCTTTGCTC 003280
003281 TTTTTTTGGG GTGGGGGGGA GATAGAGTTT CACTCTGTCA CCCAGGCTGG AGTGCAGTGG CACAATCTTT GCTCACTGTA 003360
003361 ACCTCTGCCT CTTGGGTTCA AGTGATTCTC GTGCCCCAGC CTCCCGAGTA GCTGGGACTA CAGGCACGTG CCACCACGCC 003440
003441 CAGCTAATTT TTATATTTTT AGTAGAGACG GGGTTTTGCT GTGTTGGCCA GGCTGGTCTC AAACTCCTGA CCTCAAGTAA 003520
003521 TCCACCTGGC CTGCTCTTTT CATGTCTTAA CATGGCATGT CTTTTAGTTT CATTATTTTC CTACTCCTTG TATGTCAAGA 003600
003601 AATTACATTT TGCATGTCTT ATGGAGATGC TGTTAATTGC TTCAGTGAGT GCTTTTCTAA TCTGCAGACC ATTTACATTT 003680
003681 CCTGTTTGCA GCATGCTGTG TGCAAACACT CAGTAATTTG GAGTATTCAA TTATTTGTTA GGGCTCTTCC TATTTCCAAA 003760
003761 TGTGCTGAAT TGTCTATTGA TGGGATTTTC AGATCTTTTC ATGAGAACTG GAAATGTAGC TGGGTGGCAC CTACCTAGGT 003840
003841 TGCTACGTAG TGAGTAGACT TTCTCTTGGG TATAGTAAGC CTCAGACAGC TTTCACTTTT ATCTACTTTA CTTGTGGAAA 003920
003921 TAAAACAGTC ATTTTGTTCT GAAAGAATAA GATAGCTTTC TGTAGAGAAG GAATTCCTAC CTCTAAAAGC TGCCTTGAGA 004000
004001 ACTCAGAACT GGCAGTTTTC TGAGGTGATT TTTAAATTTC AGTATTAGGG AGAGTCCAGC ATTTGCTGAC ACAGATTCTA 004080
004081 CATAACTAAT GTATGATAGC AAATGCAAAA CTATTATAAT GTGGTGTATC TTGCGCATAC ACAGGTTAGA ACAAGTAGAC 004160
004161 TCTGGCAGCA GATCTCCAGA GACCCAAGTT TAGGTTCTCA TAGTGTATTT GAAGTAGTTA TACTCCTGGC TTAAGTAGTT 004240
004241 TAGTGCCTGG GAGAATCCAT TACTGAAAAG CATTTAACTT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAC TGAAAAGGTA 004320
004321 GTGAATACAG AATAGCACAA TATATAGGAA AGGATATTTC CTGTTGAAAA AAAAAGCCAT AAAATTAATA GTAATTTTCA 004400
004401 AGAGGTAGAA TTTTAGTAGC AATAAGTTTG TGCATGTATA GTAATTTGCA TTAGCAAGGT TGTACAATAG AATAAGTGAT 004480
004481 ACTACTGGAG TTTGTGGGGC TCTGCAACCA AGAGTTGCAC AAGGGCCAGG TGCGGTGGCT TACACCTATA ATCCCAGCAC 004560
004561 TTTGGGAGGC CAAGGTGGGC GGATCACAAG GTCAAGAGAT CAGGACCATC CTGGCCAACA TGGTGAAATG CCATCTCTAC 004640
004641 TAAAAATACA AAACTTAGCT GGGCATGGTG GCACATGCCT GTAATCCCAG CTACTCAGGA GGCTGAGGCA GTAGAATCAC 004720
004721 TTAAACCTGA GAGGCGGAGG TTGCAGTGAG CTGAGATCAC ACCACTGTAC TCCAGCCTGG TGACAGTAAG ACTCCACCTC 004800
004801 AAACAAACAA AAAATGCACA AAAGGAAAAA ATGTTCTGGA TAAAGAGCAT ATGAAAGGTA CTTTCTTTTA GAACAGTATG 004880
004881 AATGTATATG AATTTATCTT TATGTGAGAA ATAATCTTTT TTGTAAGTTA AAGGTTAAAG ACCCTGCTTG TTTCCTAAAT 004960
004961 CAGAGGGTTA GAGCTGTTGA AATTAAACCT TTATAGTGCT TAACCTGGAA ACAAATGTAC TACAGCCCCA CTGAAGCCGC 005040
005041 TCTATGGGTT TTTTGCTTAC GATGTTTTAG CAACAGGCAT TTATTTTGTA GAATGGTATA CTTTCTGCTT TGTCTCCTCA 005120
005121 TCTTTCTCCA CTGGTTTCCT GGGTACATTT TTTTTAAAGG GAGAGAAGTT TGTGAGTTTA ATGTTTTAAA AAAACTCCCA 005200
005201 AGAACATGGA TAAGCCGGAT TTCTCTCATG CTTATGATTA GGGAGTTAGG ATTTAAAGAT GCAAAGCAGA AGGACTGAAA 005280
005281 GGAATAGCCA GTGAATATGT TTCAGTGGGT GAGGTGTGAA ACCTTGTCTA ATATAGATGT GTCCTATGGC CTGTGACTGC 005360
005361 TTATTCTTTA TCAATGAGAA CTCAGCAAAC TAGTTTCTTT CTTGATCTGA GGAACCACAC AGCTCACATC AGAATATAAT 005440
005441 AGTGGAACTA GGGTGACTTC ACTCCCTTTC ACCTGATGTC TATCTTGGCC TTTTAGCACC TTGACTATCC CTGAAAAGAC 005520
005521 TGGGTCTTTG TTTTCCCAGG GAAAAAACGA AGTGATGTAG ATCTAAGAAA TAGTGCCTCT TTAATATTTA ATTCTTACAT 005600
005601 GACAACTAAC ACACAGGGAA AAGGCTATGT TAAGTTGATT ATAGCTCCTC TTAAAATGTC CTATGCTGTC AGTTGGTCTT 005680
005681 AGAGACATGG GAATAACCAA GCAATATTCA GACATCTGCA CTATCTGGGG ACAGCGCTAC ATAGTACAAT GATGTCAGCA 005760
005761 GTTCAGTTAC TTGGAACAAT TAAAGATTAC CCTTAACAGC CATGTTAATA CATCTAAATG CTAAAATATA CTACGAGTTT 005840
005841 TTCATAGTCT TGAAAGTATA CAGTTAATTA CTTTTCAAAG TTACTGTGGT CTCATGTTTA CTCTTCTTGT ATCTGTGATA 005920
005921 TGCAAAAAGA TGAAGACTCT TGGCCTCCAG GAGTTTACAT TCTCATGGTG CTGTTGGTTC AAGCAGATTG CTTTAGTTTA 006000
006001 TTAATGAACA TTGCTTGGCT ATTAATTATA TCCTATTTGG AAGGATCCCT TGGTGAACAG TTTTAAAAAG CAGAGGGCTG 006080
006081 TGCTAAATTT CAGGGTATTG ATAGCTTGAG TTTACATTGT ATTAGCCCTG CTCGTTATCA TTTTTTTCCC CAGGGAGCTA 006160
006161 TGCAGGTAAT GCTCATTAGC ATGAATCAGA AAAGAAACCA TTCTGCCTAA GAGCATCTTA ACCATCCCCC TAAACCACCT 006240
006241 ATGCTCTCCT GTTATAGTTG TCAGTAAATC ACGAAGAAAA TTAACAGCTC CTTAAGACTC TACATCCCTC AATTCTCTTT 006320
006321 CTTTTCCCAG AGTTTGTACA TCATTCTCTA CTCAGATGGA CCAGGATTTG CATATAAGCC TTTTAAAACT GACTAGTGGT 006400
006401 CCTAATTTTA ACATTTTTGT GTATGACTCC TATCCCTTTT CTGAAGCAGC ATTGTGATAG ATGTGGAGCT TCTACCTGCA 006480
006481 TTTCTAAGCA ATTATTAACC CAACTTTTGA GTTGAAACTT GCATGGATCC TTGTTATGTC CCGTAATTCC TCTGAATCAA 006560
006561 GGAATTTATT TTGCATGCTT ATTAAACTCA AACTGGGTCT GATTGAAAGT GCAAGCAATA GTGACAGGAC CTTAACTCTG 006640
006641 GTTTTAGAGT ACGAACATTC TAATGCATGA CTCAAAATGT CCAGTGTTGT GGTTACTAGT TATTTTATAT ATGCATTTGT 006720
006721 TAATAATGAA GCAATAGAGA CTAAGACCAA TAGCTTGAAT ATATTCAGAT TATTTTCATG TTTTAATAAA GGATTTTTCT 006800
006801 TACTTCCATT GAAAATGGGA AGTAGAAGAT AAACAGGGGC AACCACAAGA CATTCAAACT GTCAGGACTA GGGCGTAGCA 006880
006881 GTGCCCACCC TCTAACCACT TGCCTGGTTC CATTAGTCCC CAAGCATGTT TGAAAACAAA AGACACAAAC TAAGCATGCT 006960
006961 GAATAAAACT AGCTGCTATG GCTGTAATAA TCAGAAAGGT GCAGAGTTTC AGAAAGAGTA GGAAAGTAGG GAATGCCAGT 007040
007041 ATGGAGTATG TAAAATAACT AGATATGGAT ATGGGGTTTG TTTATGATAT GCTTTATGTA GACAAAGTTT GAGGTCTTTT 007120
007121 AAAACTGGGA TTTGAGTTGA AAAGAGTCAG GATGCACATA TTTCCACTAT TTCAGGATTT TCAGATGACT AGGCACATGT 007200
007201 TGTGGATGAT ACTGGTACCA ACTACCAATG ACCAACTATA AAGTACCCCT GCAATAAGGG AGACTTCCTT TTTGTAGAAT 007280
007281 AGTCCTGAAA ATACTGACAG ATCTGTGGTT AGTTTGTTAT TTTATTGCAT AAAAAACAGT TTAAACAAAT TTTATAGCCA 007360
007361 AAGTTTTATC CTTGATGGGT TTGGCCAGAC TGCAATTTCT TGACTAAAGC TTTTAATGCC AGGTTAAACA GGAGAAACTT 007440
007441 TTTCCACTAG AAGAAAATCC TTGCTATCTA TTTTTTCCAA TAGAAGAAAA TCCTTGCTAT TTATTTTATT TGATGAATAA 007520
007521 ACAAATTTAT TGCAGTAGCT TAAAAAAATT TTTTTTTTAA ACAGTCTCAC TCTGTCGCCC AGGCTGGAGT GAAGCAATGT 007600
007601 GATCTCAGCT CACTGCAACC TCCACCTCCC GAGTAGCTGG GATTACAGAC ATGCACCACC ACCCTCAGCT AATTTTTGTA 007680
007681 TTTTTAGTAG AGACGGGGTT TCGCCATGTT GGCCAGGCTG GTCTTTAACT CCTGGCCTTA CGTGATCCGC CCCCCCTTAG 007760
007761 CCTTCCAAAG TGCTGGGATT ACAGGTGTGA GCCACTGCAC CTGGCCTGTA GTAGCTTAAA ATTTTCCTTG AGAAAATTCC 007840
007841 TGACTTTAAA AATAACCCTT ATATAAGTAC AAGTGATTGT GACAAATGAC GTAAAAATGG CATTCATGAT GTCTGAAACA 007920
007921 AGCCTAAATA GAATTCAAGA TTAGACTAAA TGATTTTCAC AAAGCACATT CAAGGTTTTA CATTCTATGA TTGAAAAAAA 008000
008001 TTTTTTGAAA ACTTTTTATT TCATTCTTTC CTGTAGGATT TTGCTACAAA TAACTTTGGG AATGAATAAA GTGGAATGGT 008080
008081 AACTTTCCAG TGGTTCAGAA TTGAATTAGA CTTCTTGTGA CTGTGATGTT TGGTTTCCAT TGAAATATAT GAAGTGAGAT 008160
008161 GTCATATCCT GAATATAGTT TGTCTTCCCC AATTACTTGA TAGCATGTCT GTCAGCCAGT AAAGATTAAG AACAGAGTTT 008240
008241 CTCTAAATTC CTCCGATTAT TCCACTAAGG CACATTAAAA TACTTAATTT TGGGAAACCA GACATCACAG ATTTCTCCAT 008320
008321 GAAGTCCTAA ATCTTCTTTA AAGTCAGAAT AGGTATCTTA GTTACTGACA GTATTCAGGT TTTTTTCTCC CTTGGTGATA 008400
008401 TGTCATTCCA TCAGTGAAAA AATATTTTCT CCCAGGGATA AGAAAGGTAT TCTGGTAATA CATTATCATC AATCCTTAAA 008480
008481 CAGTAACAGT CTTGGCACTT ATCACAAAAC CGACCCATTT CTTATAACCA GAAAGATTAT CTTAGACTGT CCTTCACATT 008560
008561 ATACTTTACC TACTGCCTTG TAAGAATAAG AGTTGCTCAC TGTGTTTACT TGCTGTCCTC CATATTCTCC ATTGCACCAT 008640
008641 TGGTGTATAA CGTTAAGAGT TTCATTGAAT ATTATTTTAA GTATTACAAA AGGCAGCTTG CTTCTTAATC TATGCATCTT 008720
008721 TGGGGTTTTT GAAGAAATTT AATTCTTTGA TGTAAAAAGG AACTGTTAAA AAAGTTGGAA GCTCTGCACC TGTGTATATA 008800
008801 TATATTTTAG CAATAAAGCA GCATGGGCTG AGAATGCACT GAAA
