Difference between revisions of "NONHSAT112472"
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| − | + | TDRG1, a developmentaly related testicular-specific gene, which was located in chromosome 6p21.1-p21.2 with 1197 bp in length, may involve in normal human spermatogenesis and fertilization. | |
==Annotated Information== | ==Annotated Information== | ||
===Transcriptomic Nomeclature=== | ===Transcriptomic Nomeclature=== | ||
| − | + | TDRG1:testis development related 1 (non-protein coding)(HGNC nomenclature） | |
| + | |||
| + | ===characteristics=== | ||
| + | TDRG1 was 1197 bp in length, located in chromosome 6p21.1-p21.2. The sequence of the open reading frame was 504-806bp. The cDNA encodes a novel protein of 100 amino acids. TDRG1 is exclusively expressed in the testis but not in any other non-reproductive tissues. TDRG1 is mainly located in spermatogenic cells in seminiferous tubules of adult testis<ref name="ref1" />. | ||
===Function=== | ===Function=== | ||
| − | + | G1 is a human testis-specific gene and may play important roles in normal human spermatogenesis<ref name="ref1" />. | |
| − | === | + | ===Disease=== |
| − | + | testicular cancer | |
===Expression=== | ===Expression=== | ||
| − | + | TDRG1 is specially expressed in spermatogenic cells, with highest expression level during human puberty stage, and decresing levels of expression with aging<ref name="ref1" />. | |
| + | Primers for regular RT-PCR and real time RT-PCR were as follows, | ||
| + | {|class='wikitalbe' style="text-align:center" | ||
| + | |- | ||
| + | ! | Primers | ||
| + | ! | RT-PCR | ||
| + | ! | real time RT-PCR | ||
| + | |- | ||
| + | | | TDRG1 | ||
| + | | | 5'-AAGAGGAGGGAGGCAGTCT-3' | ||
| + | | | 5'-GCCCAATTCCTCTTGACTGA-3'<ref name="ref1" /> | ||
| + | |- | ||
| + | | | GAPDH | ||
| + | | | 5'-CCACCCATGGCAAATTCCATGGCA-3' | ||
| + | | | 5'-TCTAGACGGCAGGTCAGGTCACC-3'<ref name="ref1" /> | ||
| + | |} | ||
===Allelic Information and Variation=== | ===Allelic Information and Variation=== | ||
| Line 23: | Line 41: | ||
==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | + | * Department of Urology, the Third Xiangya Hospital of Central South University, Changsha, Hunan, PR China.<ref name="ref1" /> | |
==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1">Jiang X, Li D, Yang J, Wen J, Chen H, Xiao X, et al. Characterization of a novel human testis-specific gene: testis developmental related gene 1 (TDRG1)[J]. The Tohoku journal of experimental medicine. 2011,225(4):311-8.</ref>(1) | ||
| + | </references> | ||
{{basic| | {{basic| | ||
tID = NONHSAT112472| | tID = NONHSAT112472| | ||
source = NONCODE4.0| | source = NONCODE4.0| | ||
| − | same = ,| | + | same =TDRG1 ,| |
classification = intergenic| | classification = intergenic| | ||
length = 1085 nt| | length = 1085 nt| | ||
Latest revision as of 14:33, 1 July 2016
TDRG1, a developmentaly related testicular-specific gene, which was located in chromosome 6p21.1-p21.2 with 1197 bp in length, may involve in normal human spermatogenesis and fertilization.
Contents
Annotated Information
Transcriptomic Nomeclature
TDRG1:testis development related 1 (non-protein coding)(HGNC nomenclature）
characteristics
TDRG1 was 1197 bp in length, located in chromosome 6p21.1-p21.2. The sequence of the open reading frame was 504-806bp. The cDNA encodes a novel protein of 100 amino acids. TDRG1 is exclusively expressed in the testis but not in any other non-reproductive tissues. TDRG1 is mainly located in spermatogenic cells in seminiferous tubules of adult testis[1].
Function
G1 is a human testis-specific gene and may play important roles in normal human spermatogenesis[1].
Disease
testicular cancer
Expression
TDRG1 is specially expressed in spermatogenic cells, with highest expression level during human puberty stage, and decresing levels of expression with aging[1]. Primers for regular RT-PCR and real time RT-PCR were as follows,
| Primers | RT-PCR | real time RT-PCR |
|---|---|---|
| TDRG1 | 5'-AAGAGGAGGGAGGCAGTCT-3' | 5'-GCCCAATTCCTCTTGACTGA-3'[1] |
| GAPDH | 5'-CCACCCATGGCAAATTCCATGGCA-3' | 5'-TCTAGACGGCAGGTCAGGTCACC-3'[1] |
Allelic Information and Variation
Please input allelic information and variation information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this lncRNA
- Department of Urology, the Third Xiangya Hospital of Central South University, Changsha, Hunan, PR China.[1]
References
Basic Information
| Transcript ID |
NONHSAT112472 |
| Source |
NONCODE4.0 |
| Same with |
TDRG1 , |
| Classification |
intergenic |
| Length |
1085 nt |
| Genomic location |
chr6+:40345782..40347638 |
| Exon number |
2 |
| Exons |
40345782..40346636,40347021..40347638 |
| Genome context |
|
| Sequence |
000001 AAGATCAGGA CTGCTGAAAG AATGAAGAAG AACTTACTTG GCCTAGGATG TGGCTAGAGA ACGGAGCGCA CTTTCACTTA 000080
000081 CCTGGCAAGG TGGTTTTGGA AGTTTCCACG GAAGCCTTCC CAGGGCCGCT GCTGCCCGTC CTCGCCTAGG TGTGGAGCTT 000160 000161 CCCGACCGGC TGGGGAGGGA ATGTCCTGCG GGAGCCGCCG AGGACCCTCT TTAGCCTCTT CCCTGGCTTG GCTGCAGCTG 000240 000241 CAGTCTGGTA CTCGCCCATT CTGCTCTTCT TCACCTCCGT ATTTTCTCTC TCACGGAAGA AGAATTCCAT TCTCCCATCC 000320 000321 CAGGAAGGAG AGTGCCCTGC GTGCCACGAA AGAGCCCAGG ACCCACAGGA CAAATACGTC ACTGGCAGAC CTGCCTTCGC 000400 000401 CAGCGCCAGC CCATCTCTGA AACCTCCAGC ATTTGTGCCC CGGTCCGGCC CCTCCCAGGC CTGACTCTTT CCGTGAACGG 000480 000481 TCACTGCGCA GGATCAAGCT ACAATGAAGA GGAGGGAGGC AGTCTGCGCG CACCGCCATT TTCTAGGAAC TGGGAAGCCC 000560 000561 CCCCACCCCT TAGGAAGATC CATCCCTGTG GAACCTTGCC CAGGCTTACC AGCCTTTGCT GAGGTTGATC TATTGTCCCT 000640 000641 CCTTGTCCCC ATCAAAATAT CCAGCACTCC ACCTTCAGGG AGTAGACTTG ACCCTCAAAT AGCAAGTTCA GCCTTCCCAG 000720 000721 GTCTAGGTTC CCTGGGAGGT CAAGATTCGT CTGGTTCCTT AGTACAGAGG GCTAGCTGTG AGTTGGAATC CCCCTATGAG 000800 000801 CTTTAGAATC AGTCAAGAGG AATTGGGCCC CTTCCCTTCA TCCCTCTTCT TTTCCCTTTT TGTCCCAGAG CTCAGCTCTG 000880 000881 ACTCAAAAGT TTTTCCATTT ACCATCAACA TGGAAACTTG GCTCCTCACG TAGGTATATT ATCCCCCTTT TGTACATGGT 000960 000961 CTTGTTGATC CAAACTCCCT TTCTGTGAAA GAGGCCTGTG GGGCTCAAGA AGCCTGGTCA GCCAGCCAGG CTAGTCCCAC 001040 001041 ATACCTCAGA ACCAGTTTAA TAAAGGCTCT ATGTCATTCT TTTTT |
