Difference between revisions of "BLACAT1"
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''BLACAT1'',also named ''linc-UBC1'' (Up-regulated in bladder cancer 1), which physically associates with PRC2 complex, is frequently overexpressed in bladder cancer tissues. | ''BLACAT1'',also named ''linc-UBC1'' (Up-regulated in bladder cancer 1), which physically associates with PRC2 complex, is frequently overexpressed in bladder cancer tissues. | ||
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==Annotated Information== | ==Annotated Information== | ||
===Name=== | ===Name=== | ||
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===Characteristics=== | ===Characteristics=== | ||
''BLACAT1'' is a bona fide noncoding RNA and preferentially localizes in the nucleus. ''BLACAT1'' acts in trans and binds to PRC2 complex in bladder cancer cell. <ref name="ref1" /> | ''BLACAT1'' is a bona fide noncoding RNA and preferentially localizes in the nucleus. ''BLACAT1'' acts in trans and binds to PRC2 complex in bladder cancer cell. <ref name="ref1" /> | ||
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===Cellular Localization=== | ===Cellular Localization=== | ||
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''BLACAT1'' is up-regulated in human primary bladder cancer tissues and serves as a negative prognostic factor for lymph node metastasis and survival in bladder cancer patients. Knock down of ''BLACAT1'' in bladder cancer cells inhibits cell proliferation, motility and invasiveness in vitro and in vivo. ''BLACAT1'' physically associates with the PRC2 complex and could be an important regulator for bladder cancer pathogenesis and candidate for further gene therapy.<ref name="ref1" />. | ''BLACAT1'' is up-regulated in human primary bladder cancer tissues and serves as a negative prognostic factor for lymph node metastasis and survival in bladder cancer patients. Knock down of ''BLACAT1'' in bladder cancer cells inhibits cell proliferation, motility and invasiveness in vitro and in vivo. ''BLACAT1'' physically associates with the PRC2 complex and could be an important regulator for bladder cancer pathogenesis and candidate for further gene therapy.<ref name="ref1" />. | ||
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===Diseases=== | ===Diseases=== | ||
| − | * Bladder cancer | + | * Bladder cancer <ref name="ref1" /> |
===Expression=== | ===Expression=== | ||
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==References== | ==References== | ||
<references> | <references> | ||
| − | <ref name="ref1"> linc-UBC1 physically associates with polycomb repressive complex 2 (PRC2) and acts as a negative prognostic factor for lymph node metastasis and survival in bladder cancer. | + | <ref name="ref1"> He W, Cai Q, Sun F, Zhong G, Wang P, Liu H, et al. linc-UBC1 physically associates with polycomb repressive complex 2 (PRC2) and acts as a negative prognostic factor for lymph node metastasis and survival in bladder cancer[J]. Biochimica et biophysica acta. 2013,1832(10):1528-37. |
</ref>(1) | </ref>(1) | ||
</references> | </references> | ||
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Latest revision as of 08:40, 27 March 2018
BLACAT1,also named linc-UBC1 (Up-regulated in bladder cancer 1), which physically associates with PRC2 complex, is frequently overexpressed in bladder cancer tissues.
Contents
Annotated Information
Name
BLACAT1:bladder cancer associated transcript 1(non-protein coding)(HGNC nomenclature)
Characteristics
BLACAT1 is a bona fide noncoding RNA and preferentially localizes in the nucleus. BLACAT1 acts in trans and binds to PRC2 complex in bladder cancer cell. [1]
Cellular Localization
nucleus[1].
Function
linc-UBC1 knockdown attenuates bladder cancer cell proliferation. [1].
BLACAT1 is up-regulated in human primary bladder cancer tissues and serves as a negative prognostic factor for lymph node metastasis and survival in bladder cancer patients. Knock down of BLACAT1 in bladder cancer cells inhibits cell proliferation, motility and invasiveness in vitro and in vivo. BLACAT1 physically associates with the PRC2 complex and could be an important regulator for bladder cancer pathogenesis and candidate for further gene therapy.[1].
Diseases
- Bladder cancer [1]
Expression
Linc-UBC1 is frequently overexpressed in bladder cancer tissues compared with normal adjacent tissues.[1]
| Primers for qRT-PCR | Forward | Reverse |
|---|---|---|
| linc-UBC1 (lincRNA:chr1:205404014-205407007) | 5’-CCTGCTTGGAAACTAATGACC-3’ | 5’-AGGCTCAACTTCCCAGACTCA-3'[1] |
| lincRNA:chr10:4692376-4720283 | 5'-CGAGATGGAGCAAAGGATAAACG-3' | 5'-TCAGGGCCAGGAGGTGACTGGA-3'[1] |
| lincRNA:chr18:37236595-37243620 | 5'-GTTACCCTTGCCCTTTGGATG-3' | 5'-GCCTTGTAGGCCCTCACAGAC-3'[1] |
| lincRNA:chr4:15669186-15683175 | 5'-GGCCAGGACGCTGGTTCAT-3' | 5'-TCGGGAGGTTGTGGGAGGA-3'[1] |
| GAPDAH | 5’-GAAGGTGAAGGTCGGAGTC-3’ | 5’-GAAGATGGTGATGGGATTTC-3’[1] |
| HOTAIR | 5'-GGTAGAAAAAGCAACCACGAAGC-3' | 5'-ACATAAACCTCTGTCTGTGAGTGCC-3'[1] |
| U1 | 5’-GGGAGATACCATGATCACGAAGGT-3’ | 5’-CCACAAATTATGCAGTCGAGTTTCCC-3’[1] |
| CCND2 | 5’-ACCTTCCGCAGTGCTCCTA-3’ | 5’-CCCAGCCAAGAAACGGTCC-3’[1] |
| SERPINB2 | 5'-CAGCACCGAAGACCAGATGG-3' | 5'-CCTGCAAAATCGCATCAGGATAA-3'[1] |
| BMP2 | 5'-ACCCGCTGTCTTCTAGCGT-3' | 5'-TTTCAGGCCGAACATGCTGAG-3'[1] |
| KLF4 | 5'-CGGACATCAACGACGTGAG-3' | 5'-GACGCCTTCAGCACGAACT-3'[1] |
| HOXA5 | 5'-AACTCATTTTGCGGTCGCTAT-3' | 5'-TCCCTGAATTGCTCGCTCAC-3'[1] |
| KLHDC8A | 5'-TGGACTGCTTCGAGGTCTACT-3' | 5'-CTTTGGCCGTGACAGAAATGC-3'[1] |
| LEMD1 | 5'-ATTGCAGAACCAACTTGAGAAGC-3' | 5'-CGCGCAGTAGTCTCTCTCTT-3'[1] |
| CDK18 | 5'-CAAAGGGCGCAGCAAACTG-3' | 5'-AGGTCATGCAGGGTCACAATA-3'[1] |
| EZH2 | 5'-AATCAGAGTACATGCGACTGAGA-3' | 5'-GCTGTATCCTTCGCTGTTTCC-3'[1] |
| SUZ12 | 5'-TTGCAGCTTACGTTTACTGGTT-3' | 5'-GGAACTTGCCTTATTGGACAACT-3'[1] |
Sequence
>gi|34365321|emb|BX640973.1| Homo sapiens mRNA; cDNA DKFZp686B15184 (from clone DKFZp686B15184)
000001 GGGGATGCAA GGAGCACTGA GCTGGGAGCC AGGTCTCCTG GCTTCTAGCC CTGCTTGGAA ACTAATGACC TATGTGACCT 000080
000081 TCGACTAGTC ATGTCTTATT TCTGAGACTC AGGTTCCTCA ACTATAAATA GGGGATTACA TAAATTTCCT CTTAAGTCTC 000160
000161 CTTTGGAGTC CAGGGCTCTG TGAGTCTGGG AAGTTGAGCC TCAGTGGGGC ATAGGGTTGT GGAACCAGAG GGTCGCAGGT 000240
000241 TTCCTCTGCC CTCACAGATG CACTCCGTGC AGACAACAGA CATATCTGGA GGGTGGATGG AAGTGGTATG GTGGCTCTGA 000320
000321 GAAGTGAGAG GGGGAGTGAG AGGGAAAGGC AAGTCACCCA GAGCTTCTGC TAGAATCTTC CCCTAGCCAG GGCCCAGGAC 000400
000401 TCCTTGCAGT TCTGCCTCTC TTCCCTCCTA CAGCCAGGCC CAAGGGGAGT AAGTCAGTTG GCCTAGACTC TGACTCCAGG 000480
000481 TCCTGTCTAG GCCATCACAG GCGCCACTCT TATGTACGTT CTCACTTTCT CTCTGTCACA TACACACAGC ATCACCACCA 000560
000561 CCACCACCAC AACCACCTGC AGATAGATGC TCAAGTGATT CTAGAAGAGA TCTCAAGGAC TGGCGCTCTG CTCCAGTTAT 000640
000641 GTAACCCATG GGCTTCCCTT TGCACAGCTC CTCATGTGCT CATCTCCATG CTGATCAAAC CACCCCACCT CATTCCTTGC 000720
000721 CTTCTGCAAG GGGAAGTGCC TCAGGCTGGA GTTTGTCCTG TCTACAGACT GAATATGCAT TTTGCCCAGT GGCTGGAGAT 000800
000801 GTGCTGGTGG TGGAATGTGC TGGTGATTGT TTCTGGATGG AGAGCCACAG CCCCCCAGTG TTCTGCCATG ACTGACTCCT 000880
000881 GACCTTGGCA AATTGCCTCC TCTCTCCCAG CCTCCTCCAT AACAGGTGGC GATGAGTCCC ATTTCCTCCC TACCTCACAG 000960
000961 GAAGGCACGC ATTATAAAGG GCACACTTAT CAGATGTGCC CAGATGTGGC TGGAACTTTT GGAGACAGAT ACTTGGTTCA 001040
001041 CAACCGCAAA GGTGGGCTTT CACCAGAGAA GAAAATCTCT GGGCTGCTGA GTTCAGTGGG CTCCTTGCAG GCTGGTCCAG 001120
001121 GAAGTCTGGT GTTCCTCCTG AAGGTGGCTG CTATGTCCAG CAGGTGATCA AGCTGACAGT ACCACAAGAA CCAAGAAACA 001200
001201 GAATCTGTTC CCAAGGAATG AATGTTCTAT CTCTCTGACC CAAAAGCAAG AGCAAGAGAG AGCTGTCACC ATCATCACCC 001280
001281 TGCTCCCAAC ACAAACACAG CCCCCCAAGT CCATATTGCT GACAAGGGTA TCTGCCTTGT CAGGGTGAAT CCTCTGGACC 001360
001361 CCCAGGCCAT CCCTGGAGTG GTCAGTGCCC ACTGTGCCGG GGTTATTGTG GCCAGTCCGC CTGGGTTCGC TTCTCTAAAT 001440
001441 TTCCATGGGA TAGGAACTAT AGATCATGAA GAAGAAACAG GAACCTTGAC TCTTCACGGA GGAGCCCAGG AGGTCAGAAC 001520
001521 CATCCACCAG GAGGGTGAGC GGTAGCTGCC CAGGGCATCC CTGGAGGAAG TCGTCATACT GAGGAAGAAA TTGCCAAAGC 001600
001601 CAAGAGCTCC CAGACCTGGG CAGCTCAGGA GCTGACAGAA ACCACCCCCC AAGTCTGGAG TTCAAGGGTT TTCCTCGTGG 001680
001681 GCTGCTGGTC TGTCTCTTTC CCTTCTGGGG CAGCCGTCTC TCCTCCTTCA GCTCATTCCC CGTGTTCCAT CTGAGGGGCG 001760
001761 GGAGGAAGGC AGGAGGGGGA GATGGCAGGA CCCAGACAGA TCCTCAGGAC TCTCCCAGCC TCTGTTGCCA TGGCAACACT 001840
001841 GCTATTTTTA TCCCGTTGTT CATGATGCTC AAAGCTCTAA CTTTCTCTGT TCCAGAGAGG CCAGGCTGGT TTCTGCCCTC 001920
001921 ATCCTTTAAC ACAGCATCTT CTCCCAGAGG CCTGAGGATG GGAAAAAGTG ATGGAGAAAA GGGGAACCCC TAAGGTCACC 002000
002001 CCTCAGCCAG GGGGAACTGT TTAACAGGGG TTTGTCTCTG CCCTTTTGAG CCTTTGGTTT TCTACCTGGC TCAGGCACCC 002080
002081 AGGTTTATGT TTTCTAGATC AAAACTCTGC ATGGTCTCCC TGAGAGACTG GAGAAGAGCT GCCAATCAGG ATGGATTTTT 002160
002161 TCCTACACAG GTATGACACT GCAGCCACTT CCCTTCTTGG GAAAATTAAA CTTCATTCTG AAGAATGTCC TGTTGGTGGT 002240
002241 ATAAGCCAAC CCCTGCCCTC CTAAAAGGGG ATAGATAATT AAATTTGGTT AATTAGCAAT CAGATTCACT GGGGAATTGA 002320
002321 AACCCGTACT TCCCTTTCTT CTGTCCCTCC ACCAAAGAAC CGGAACAAAT GGCTTCATGT CTTTGTCCAG TCTTTCTGCC 002400
002401 TAAAGCAAAA TAGCATATAT TCTCCAGGCT GAAAAGTAGA TTCCTCCTCA CCCCTACTCA AGACGGCCAC ATTTCAATCC 002480
002481 AGGCTCTTGA AGACAGGGTC TAATGAAAGG ATTTCTGTTC TTTCTCTGTC TGTTCCCTCT GGCCCAGCTT CTAGTCCTCT 002560
002561 CCTTATGTCC CCACCTCCAT GGCAGTGGCA AGAGACCCCT GAGAGTCAAT GGTGCTAACG TTCCCCACCT ATATACCCCT 002640
002641 ATGTAGTTTA TCGAGATTTG AACCCAGCAC CTGGGTGGCT GTGTTGGGCG GATACATTTC ACGTCCTCAA GACCACAACC 002720
002721 CTGGCGACAG GTCTGGATTA ATTCTGTAAA GTAGCAATCT ATATCATGCT AAATTCCTGC ACTGAGGTTC TACCTCGGCT 002800
002801 GAACTGACTC GTGGATGGTT AAGTTTCCTG GGGTGGCTGG AACCCATTTA CTAAGCCTCT AATTGGTTTC ATTTTGGCTC 002880
002881 AAGTTGCATG GTTTCCCGAA TCTATGCACC TGGAGTAAGC ACTTTTCTGG TTGCCACTGA AAAGCATGCC GGCAAACGCT 002960
002961 GCTCTGTTAA TTGCTGTAAT AAACAGACTT TATTATAAAA AAAAAAAAAA AAAAAAAAAA
000081 TCGACTAGTC ATGTCTTATT TCTGAGACTC AGGTTCCTCA ACTATAAATA GGGGATTACA TAAATTTCCT CTTAAGTCTC 000160
000161 CTTTGGAGTC CAGGGCTCTG TGAGTCTGGG AAGTTGAGCC TCAGTGGGGC ATAGGGTTGT GGAACCAGAG GGTCGCAGGT 000240
000241 TTCCTCTGCC CTCACAGATG CACTCCGTGC AGACAACAGA CATATCTGGA GGGTGGATGG AAGTGGTATG GTGGCTCTGA 000320
000321 GAAGTGAGAG GGGGAGTGAG AGGGAAAGGC AAGTCACCCA GAGCTTCTGC TAGAATCTTC CCCTAGCCAG GGCCCAGGAC 000400
000401 TCCTTGCAGT TCTGCCTCTC TTCCCTCCTA CAGCCAGGCC CAAGGGGAGT AAGTCAGTTG GCCTAGACTC TGACTCCAGG 000480
000481 TCCTGTCTAG GCCATCACAG GCGCCACTCT TATGTACGTT CTCACTTTCT CTCTGTCACA TACACACAGC ATCACCACCA 000560
000561 CCACCACCAC AACCACCTGC AGATAGATGC TCAAGTGATT CTAGAAGAGA TCTCAAGGAC TGGCGCTCTG CTCCAGTTAT 000640
000641 GTAACCCATG GGCTTCCCTT TGCACAGCTC CTCATGTGCT CATCTCCATG CTGATCAAAC CACCCCACCT CATTCCTTGC 000720
000721 CTTCTGCAAG GGGAAGTGCC TCAGGCTGGA GTTTGTCCTG TCTACAGACT GAATATGCAT TTTGCCCAGT GGCTGGAGAT 000800
000801 GTGCTGGTGG TGGAATGTGC TGGTGATTGT TTCTGGATGG AGAGCCACAG CCCCCCAGTG TTCTGCCATG ACTGACTCCT 000880
000881 GACCTTGGCA AATTGCCTCC TCTCTCCCAG CCTCCTCCAT AACAGGTGGC GATGAGTCCC ATTTCCTCCC TACCTCACAG 000960
000961 GAAGGCACGC ATTATAAAGG GCACACTTAT CAGATGTGCC CAGATGTGGC TGGAACTTTT GGAGACAGAT ACTTGGTTCA 001040
001041 CAACCGCAAA GGTGGGCTTT CACCAGAGAA GAAAATCTCT GGGCTGCTGA GTTCAGTGGG CTCCTTGCAG GCTGGTCCAG 001120
001121 GAAGTCTGGT GTTCCTCCTG AAGGTGGCTG CTATGTCCAG CAGGTGATCA AGCTGACAGT ACCACAAGAA CCAAGAAACA 001200
001201 GAATCTGTTC CCAAGGAATG AATGTTCTAT CTCTCTGACC CAAAAGCAAG AGCAAGAGAG AGCTGTCACC ATCATCACCC 001280
001281 TGCTCCCAAC ACAAACACAG CCCCCCAAGT CCATATTGCT GACAAGGGTA TCTGCCTTGT CAGGGTGAAT CCTCTGGACC 001360
001361 CCCAGGCCAT CCCTGGAGTG GTCAGTGCCC ACTGTGCCGG GGTTATTGTG GCCAGTCCGC CTGGGTTCGC TTCTCTAAAT 001440
001441 TTCCATGGGA TAGGAACTAT AGATCATGAA GAAGAAACAG GAACCTTGAC TCTTCACGGA GGAGCCCAGG AGGTCAGAAC 001520
001521 CATCCACCAG GAGGGTGAGC GGTAGCTGCC CAGGGCATCC CTGGAGGAAG TCGTCATACT GAGGAAGAAA TTGCCAAAGC 001600
001601 CAAGAGCTCC CAGACCTGGG CAGCTCAGGA GCTGACAGAA ACCACCCCCC AAGTCTGGAG TTCAAGGGTT TTCCTCGTGG 001680
001681 GCTGCTGGTC TGTCTCTTTC CCTTCTGGGG CAGCCGTCTC TCCTCCTTCA GCTCATTCCC CGTGTTCCAT CTGAGGGGCG 001760
001761 GGAGGAAGGC AGGAGGGGGA GATGGCAGGA CCCAGACAGA TCCTCAGGAC TCTCCCAGCC TCTGTTGCCA TGGCAACACT 001840
001841 GCTATTTTTA TCCCGTTGTT CATGATGCTC AAAGCTCTAA CTTTCTCTGT TCCAGAGAGG CCAGGCTGGT TTCTGCCCTC 001920
001921 ATCCTTTAAC ACAGCATCTT CTCCCAGAGG CCTGAGGATG GGAAAAAGTG ATGGAGAAAA GGGGAACCCC TAAGGTCACC 002000
002001 CCTCAGCCAG GGGGAACTGT TTAACAGGGG TTTGTCTCTG CCCTTTTGAG CCTTTGGTTT TCTACCTGGC TCAGGCACCC 002080
002081 AGGTTTATGT TTTCTAGATC AAAACTCTGC ATGGTCTCCC TGAGAGACTG GAGAAGAGCT GCCAATCAGG ATGGATTTTT 002160
002161 TCCTACACAG GTATGACACT GCAGCCACTT CCCTTCTTGG GAAAATTAAA CTTCATTCTG AAGAATGTCC TGTTGGTGGT 002240
002241 ATAAGCCAAC CCCTGCCCTC CTAAAAGGGG ATAGATAATT AAATTTGGTT AATTAGCAAT CAGATTCACT GGGGAATTGA 002320
002321 AACCCGTACT TCCCTTTCTT CTGTCCCTCC ACCAAAGAAC CGGAACAAAT GGCTTCATGT CTTTGTCCAG TCTTTCTGCC 002400
002401 TAAAGCAAAA TAGCATATAT TCTCCAGGCT GAAAAGTAGA TTCCTCCTCA CCCCTACTCA AGACGGCCAC ATTTCAATCC 002480
002481 AGGCTCTTGA AGACAGGGTC TAATGAAAGG ATTTCTGTTC TTTCTCTGTC TGTTCCCTCT GGCCCAGCTT CTAGTCCTCT 002560
002561 CCTTATGTCC CCACCTCCAT GGCAGTGGCA AGAGACCCCT GAGAGTCAAT GGTGCTAACG TTCCCCACCT ATATACCCCT 002640
002641 ATGTAGTTTA TCGAGATTTG AACCCAGCAC CTGGGTGGCT GTGTTGGGCG GATACATTTC ACGTCCTCAA GACCACAACC 002720
002721 CTGGCGACAG GTCTGGATTA ATTCTGTAAA GTAGCAATCT ATATCATGCT AAATTCCTGC ACTGAGGTTC TACCTCGGCT 002800
002801 GAACTGACTC GTGGATGGTT AAGTTTCCTG GGGTGGCTGG AACCCATTTA CTAAGCCTCT AATTGGTTTC ATTTTGGCTC 002880
002881 AAGTTGCATG GTTTCCCGAA TCTATGCACC TGGAGTAAGC ACTTTTCTGG TTGCCACTGA AAAGCATGCC GGCAAACGCT 002960
002961 GCTCTGTTAA TTGCTGTAAT AAACAGACTT TATTATAAAA AAAAAAAAAA AAAAAAAAAA
Labs working on this lncRNA
- Department of Urology, Sun Yat-sen Memorial Hospital, 107th Yanjiangxi Road, Yuexiu District, Guangzhou, Guangdong province, PR China. [1].
References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 He W, Cai Q, Sun F, Zhong G, Wang P, Liu H, et al. linc-UBC1 physically associates with polycomb repressive complex 2 (PRC2) and acts as a negative prognostic factor for lymph node metastasis and survival in bladder cancer[J]. Biochimica et biophysica acta. 2013,1832(10):1528-37.
