Difference between revisions of "MDC1-AS1"

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(Created page with "==Annotated Information== ===Approved Symbol=== MDC1-AS1 ===Approved Name=== MDC1 antisense RNA 1 ===Previous Symbols=== _ ===Synonyms=== MDC1-AS ===Chromosome=== 6p21.33 ===R...")
 
 
(9 intermediate revisions by one other user not shown)
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===Approved Name===
 
===Approved Name===
 
MDC1 antisense RNA 1
 
MDC1 antisense RNA 1
−
===Previous Symbols===
 
−
_
 
 
===Synonyms===
 
===Synonyms===
 
MDC1-AS
 
MDC1-AS
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===RefSeq ID===
 
===RefSeq ID===
 
NR_133647
 
NR_133647
−
===OMIM ID===
 
−
_
 
 
===Ensembl ID===
 
===Ensembl ID===
 
ENSG00000224328
 
ENSG00000224328
 
===pubmed IDs===
 
===pubmed IDs===
 
25514464
 
25514464
 +
===Cellular Localization===
 +
MDC1-AS primarily exists in the nuclear fraction <ref name="ref1" />.
 +
 +
===Function===
 +
MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.<ref name="ref1" />
 +
 +
MDC1-AS exhibits significant decreases in proliferation,colony formation, migration and invasion.<ref name="ref1" />
 +
 +
===Diseases===
 +
MDC1-AS may act as a tumor suppressor in bladder cancer <ref name="ref1" />.
 +
 +
===Expression===
 +
[[File:Dysregulation of MDC1-AS and MDC1 expression level.jpg|right|thumb|600px|''(A) MDC1-AS levels in cancerous tissues were significantly lower than those in the noncancerous tissues. (B) MDC1 was down-regulated in bladder cancerous tissues, compared with that in adjacent normal tissues. (C) Expression levels of MDC1-AS and MDC1 in tissues were significantly correlated in a positive direction. (D) Both MDC1-AS and MDC1 were decreased in bladder cancer cells T24 and EJ, compared with those in normal cell line 293A.''' <ref name="ref1" />.]]
 +
 +
MDC1-AS levels in cancerous tissues are evidently lower than those in the noncancerous tissues <ref name="ref1" />.
 +
 +
MDC1 is down-regulated in bladder cancer tissues, which was concordant with MDC1-AS <ref name="ref1" />.
 +
 +
Expression levels of MDC1-AS and MDC1 in tissues are significantly correlated in a positive direction, indicating a potential regulating role of MDC1-AS on the coding gene MDC1 <ref name="ref1" />.
 +
 +
The expression levels of MDC1-AS and MDC1 are decreased in bladder cancer cells <ref name="ref1" />.
 +
 +
 +
{| class='wikitable' style="text-align:center"
 +
|-
 +
! | Experiment
 +
! | Primer
 +
! | Forward primer
 +
! | Reverse primer
 +
|-
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| rowspan="3"|RT-PCR
 +
| | MDC1-AS
 +
| | 5'-TCCCAGATGTGCCAAAGTCAG-3'
 +
| | 5'-AGCAACCCCAGTTGTCATTC-3'<ref name="ref1" />
 +
|-
 +
| | MDC1
 +
| | 5'-GCAGCTTCCAGACAACAG-3'
 +
| | 5'-TACCCATGACTTTATCCACA-3'<ref name="ref1" />
 +
|-
 +
| | GAPDH
 +
| | 5'-AAGGTGAAGGTCGGAGTCAAC-3'
 +
| | 5'-GGGGTCATTGATGGCAACAATA-3' <ref name="ref1" />
 +
|-
 +
| rowspan="1"|RNAi
 +
| | siMDC1
 +
| | 5'-GCGGCUACAUAUCUUUAGUTT-3'
 +
| | 5'-ACUAAAGAUAUGUAGCCGCTT-3'<ref name="ref1" />
 +
|}
 +
 +
 +
 +
 +
 +
 +
==Labs working on this lncRNA==
 +
* Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer
 +
Center, Nanjing Medical University, Nanjing, China
 +
 +
==References==
 +
<references>
 +
<ref name="ref1">Xue, Y., G. Ma, Z. Zhang, Q. Hua, H. Chu, N. Tong, L. Yuan, C. Qin, C. Yin, and M. Wang. "A Novel Antisense Long Noncoding Rna Regulates the Expression of Mdc1 in Bladder Cancer." Oncotarget 6, no. 1 (2015): 484-93.</ref>
 +
</references>
 +
 
===Sequence===
 
===Sequence===
 
>gi|949474747|ref|NR_133647.1| Homo sapiens MDC1 antisense RNA 1 (MDC1-AS1), long non-coding RNA
 
>gi|949474747|ref|NR_133647.1| Homo sapiens MDC1 antisense RNA 1 (MDC1-AS1), long non-coding RNA
 
<dnaseq>CACTATATCGGTCTGTCATCATCCCTTGGCCTCTCACCTGATGCAGCCAGTCCAGGGACAGAATGGGGATTCCCCGCCCCAGGGCACACAGGAACTTGACTGTCCGGCGGATGCGATCAGTGACCAGACACCTGATGCTTCTTTATATGTATAATGGCTGACCCTGGCGGGACTTCCTTCTCCACTTGTGTGTTGATGTCCACTGTGGTGGAGGCTTGGCTTCTCTCCAGGTGGATCCCAGATGTGCCAAAGTCAGCGCTGCTGAGACTTCTTCCTCGTCATCTGTATCGCTGTTGATAACCATGGAAGCTTGGCTTTTCTCCAGAGGGACAGCCTGTGGGGCCTTGCCTTCTTCCACATCTGTATCACTACCAGCCTGGCTCTCCTGCAGATGGGCCAGGCCTGGTGCTCCAGGACCCCTTGTACCTACTCCATGGAAGATCTTCCTCTTCTTCATAGGAATGACAACTGGGGTTGCTGGGATCCTCTCTTCTTCCGCATCAGTGTCGCTGTCGATGAAGCCAAAAGGCTGAGCCCTTTCCAAATGGACCTCAGCTGGCCTTCCAGGAGGCCTGCTGTCATCATCCACATCTGTGTCACTGTCCTCTCCAGGAGGTTGGCTCCTCTCCAGAATCACCCCAGCTGGAACCACCCCATTCCCTGCACCCCTCTTGACTTTTGTATCATTGTCCCTCTCCTTCACTAAAGGCTGATCCTTTTCAAGCTGGATTTCAGTTA</dnaseq>
 
<dnaseq>CACTATATCGGTCTGTCATCATCCCTTGGCCTCTCACCTGATGCAGCCAGTCCAGGGACAGAATGGGGATTCCCCGCCCCAGGGCACACAGGAACTTGACTGTCCGGCGGATGCGATCAGTGACCAGACACCTGATGCTTCTTTATATGTATAATGGCTGACCCTGGCGGGACTTCCTTCTCCACTTGTGTGTTGATGTCCACTGTGGTGGAGGCTTGGCTTCTCTCCAGGTGGATCCCAGATGTGCCAAAGTCAGCGCTGCTGAGACTTCTTCCTCGTCATCTGTATCGCTGTTGATAACCATGGAAGCTTGGCTTTTCTCCAGAGGGACAGCCTGTGGGGCCTTGCCTTCTTCCACATCTGTATCACTACCAGCCTGGCTCTCCTGCAGATGGGCCAGGCCTGGTGCTCCAGGACCCCTTGTACCTACTCCATGGAAGATCTTCCTCTTCTTCATAGGAATGACAACTGGGGTTGCTGGGATCCTCTCTTCTTCCGCATCAGTGTCGCTGTCGATGAAGCCAAAAGGCTGAGCCCTTTCCAAATGGACCTCAGCTGGCCTTCCAGGAGGCCTGCTGTCATCATCCACATCTGTGTCACTGTCCTCTCCAGGAGGTTGGCTCCTCTCCAGAATCACCCCAGCTGGAACCACCCCATTCCCTGCACCCCTCTTGACTTTTGTATCATTGTCCCTCTCCTTCACTAAAGGCTGATCCTTTTCAAGCTGGATTTCAGTTA</dnaseq>

Latest revision as of 08:27, 19 September 2016

Annotated Information

Approved Symbol

MDC1-AS1

Approved Name

MDC1 antisense RNA 1

Synonyms

MDC1-AS

Chromosome

6p21.33

RefSeq ID

NR_133647

Ensembl ID

ENSG00000224328

pubmed IDs

25514464

Cellular Localization

MDC1-AS primarily exists in the nuclear fraction [1].

Function

MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.[1]

MDC1-AS exhibits significant decreases in proliferation,colony formation, migration and invasion.[1]

Diseases

MDC1-AS may act as a tumor suppressor in bladder cancer [1].

Expression

(A) MDC1-AS levels in cancerous tissues were significantly lower than those in the noncancerous tissues. (B) MDC1 was down-regulated in bladder cancerous tissues, compared with that in adjacent normal tissues. (C) Expression levels of MDC1-AS and MDC1 in tissues were significantly correlated in a positive direction. (D) Both MDC1-AS and MDC1 were decreased in bladder cancer cells T24 and EJ, compared with those in normal cell line 293A.' [1].

MDC1-AS levels in cancerous tissues are evidently lower than those in the noncancerous tissues [1].

MDC1 is down-regulated in bladder cancer tissues, which was concordant with MDC1-AS [1].

Expression levels of MDC1-AS and MDC1 in tissues are significantly correlated in a positive direction, indicating a potential regulating role of MDC1-AS on the coding gene MDC1 [1].

The expression levels of MDC1-AS and MDC1 are decreased in bladder cancer cells [1].


Experiment Primer Forward primer Reverse primer
RT-PCR MDC1-AS 5'-TCCCAGATGTGCCAAAGTCAG-3' 5'-AGCAACCCCAGTTGTCATTC-3'[1]
MDC1 5'-GCAGCTTCCAGACAACAG-3' 5'-TACCCATGACTTTATCCACA-3'[1]
GAPDH 5'-AAGGTGAAGGTCGGAGTCAAC-3' 5'-GGGGTCATTGATGGCAACAATA-3' [1]
RNAi siMDC1 5'-GCGGCUACAUAUCUUUAGUTT-3' 5'-ACUAAAGAUAUGUAGCCGCTT-3'[1]




Labs working on this lncRNA

  • Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer

Center, Nanjing Medical University, Nanjing, China

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Xue, Y., G. Ma, Z. Zhang, Q. Hua, H. Chu, N. Tong, L. Yuan, C. Qin, C. Yin, and M. Wang. "A Novel Antisense Long Noncoding Rna Regulates the Expression of Mdc1 in Bladder Cancer." Oncotarget 6, no. 1 (2015): 484-93.

Sequence

>gi|949474747|ref|NR_133647.1| Homo sapiens MDC1 antisense RNA 1 (MDC1-AS1), long non-coding RNA

000001 CACTATATCG GTCTGTCATC ATCCCTTGGC CTCTCACCTG ATGCAGCCAG TCCAGGGACA GAATGGGGAT TCCCCGCCCC 000080
000081 AGGGCACACA GGAACTTGAC TGTCCGGCGG ATGCGATCAG TGACCAGACA CCTGATGCTT CTTTATATGT ATAATGGCTG 000160
000161 ACCCTGGCGG GACTTCCTTC TCCACTTGTG TGTTGATGTC CACTGTGGTG GAGGCTTGGC TTCTCTCCAG GTGGATCCCA 000240
000241 GATGTGCCAA AGTCAGCGCT GCTGAGACTT CTTCCTCGTC ATCTGTATCG CTGTTGATAA CCATGGAAGC TTGGCTTTTC 000320
000321 TCCAGAGGGA CAGCCTGTGG GGCCTTGCCT TCTTCCACAT CTGTATCACT ACCAGCCTGG CTCTCCTGCA GATGGGCCAG 000400
000401 GCCTGGTGCT CCAGGACCCC TTGTACCTAC TCCATGGAAG ATCTTCCTCT TCTTCATAGG AATGACAACT GGGGTTGCTG 000480
000481 GGATCCTCTC TTCTTCCGCA TCAGTGTCGC TGTCGATGAA GCCAAAAGGC TGAGCCCTTT CCAAATGGAC CTCAGCTGGC 000560
000561 CTTCCAGGAG GCCTGCTGTC ATCATCCACA TCTGTGTCAC TGTCCTCTCC AGGAGGTTGG CTCCTCTCCA GAATCACCCC 000640
000641 AGCTGGAACC ACCCCATTCC CTGCACCCCT CTTGACTTTT GTATCATTGT CCCTCTCCTT CACTAAAGGC TGATCCTTTT 000720
000721 CAAGCTGGAT TTCAGTTA
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