Difference between revisions of "CARMN"

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CARMEN
 
CARMEN
 
===Function===
 
===Function===
−
CARMEN is a crucial regulator of cardiac cell differentiation and homeostasis. CARMEN exhibits RNA-dependent enhancing activity and is upstream of the cardiac mesoderm-specifying gene regulatory network. Interestingly, CARMEN interacts with SUZ12 and EZH2, two components of the polycomb repressive complex 2 (PRC2).
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[[File: 1-s2.0-S0022282815300699-gr2.jpg|right|thumb|400px|'''Identification of CARMEN.''' <ref name="ref1" />.]]
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CARMEN is a crucial regulator of cardiac cell differentiation and homeostasis. CARMEN exhibits RNA-dependent enhancing activity and is upstream of the cardiac mesoderm-specifying gene regulatory network. Interestingly, CARMEN interacts with SUZ12 and EZH2, two components of the polycomb repressive complex 2 (PRC2). CARMEN is modulated in cardiac disease and required for maintaining a differentiated cardiac fate. Carmen was significantly upregulated post myocardial infarction, supporting a role for Carmen in adult heart homeostasis and cardiac remodeling.<ref name="ref1" />.
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 +
 
 +
===Disease===
 +
cardiac differentiation
 
===Chromosome===
 
===Chromosome===
 
5q32
 
5q32
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===Ensembl ID===
 
===Ensembl ID===
 
ENSG00000249669
 
ENSG00000249669
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===pubmed IDs===
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===Expression===
−
26423156
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{| class='wikitable' style="text-align:center"
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|-
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! | Primer
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! | Forward primer
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! | Reverse primer
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|-
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| rowspan="1"|CARMEN1
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| | 5'-GATGGAAAAGATGGCAGAGG-3'
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| | 5'-TAGGTGTTGGCTGAGTGCAG-3'<ref name="ref1" />
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|-
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| rowspan="1"|CARMEN2
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| | 5'-CCAACCACTCCCCAAACA-3'
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| | 5'-TTACAGCCGTTGCTCTCCTT-3'<ref name="ref1" />
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|-
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| rowspan="1"|CARMEN3
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| | 5'-GAGTAGGAGGGAGCCTTTGG-3'
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| | 5'-TTTGCCTCCATCTCCTCTGT-3'<ref name="ref1" />
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|-
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| rowspan="1"|Cloning
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| | 5'-TACCGAGCTCGGATCCtcagtgccagctgcttaaaa-3'
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| | 5'-CTGGACTAGTGGATCCaggcacagtgttagagtttgct-3'<ref name="ref1" />
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|}
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 +
===Reference===
 +
<references>
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<ref name="ref1"> Ounzain, S. et al. CARMEN, a human super enhancer-associated long noncoding RNA controlling cardiac specification, differentiation and homeostasis. J Mol Cell Cardiol 89, 98-112, doi:10.1016/j.yjmcc.2015.09.016 (2015).</ref>(1)
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</references>
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[[Category: Antisense]]
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===Sequence===
 
===Sequence===
 
>gi|563318940|ref|NR_105059.1| Homo sapiens cardiac mesoderm enhancer-associated non-coding RNA (CARMN), transcript variant 1, long non-coding RNA
 
>gi|563318940|ref|NR_105059.1| Homo sapiens cardiac mesoderm enhancer-associated non-coding RNA (CARMN), transcript variant 1, long non-coding RNA
 
<dnaseq>AGCTCATATAAGGTAAAAGGCAGAGTGGCCTCTGTGGCCAGGGAGCCGCAGGCAAGGGACTAAGGGGAGGGGGGCTCAGTGCCAGCTGCTTAAAAATGCCCCTGTGGCAGCGAGGGGCACCAGAGGCTGGGTCTAATTAGTTGAGAAGCAGTGACACCCCCAACCACTCCCCAAACAGGCTGGCTCCCGTCTCCAGGCCCCAAGGAGCCACACCTGGACCAGACCCCAGGAAAGACATGTGGTGGAAACCCTACGGCCCTGGAGCCCAAGGAGAGCAACGGCTGTAACAAGTGCACAGGCAAGGGCGCTAGCTGGTGGGAGCCACCCCGCCATGCTGATGTCAGAGAAGCAAGAACTCTGGAGAAGCAGCCTCCTGGGACCAGAGGAGGGCCAGCAGCAGGCAGCCCGGAGACAGAACTCTCACCCACGCCTCTCCAAGCCTCCCAACAGAAGACAGAGGTCCCCCACAGCCAGAGACATTTCCTGAAGACATGGGGAACACAGAGGCAGAAACAGCCCATCCACCCAGGAGCTGTCCCCCACACTGCCGGGAGCCGGCACCCAGAGCCGCCAGGTAAAACTGAGGCCACCTGGTTCAACATCACCTTTCACAGAAGGGGAAGCAGCCACAGAAAGAAGGGCCTCGTTAAGAAGTGGAACCTGGGACCCCCAAGCGGTGTCTCTCATCCTGACTGGGGATCCAGAGTAGGAGGGAGCCTTTGGTGGGGTTGGAGTCCCGCCACAGGCCACCAGAGCGGAGCAGCGCAGCGCCCTGTCTCCCAGCCTGAGGTGCAGTGCTGCATCTCTGGTCAGTTGGGAGTCTGAGATGAAGCACTGTAGCTCAGGAAGAGAGAAGTTGTTCTGCAGCCATCAGCCTGGAAGTGCCTGGCTGGTGGGCCTTCTTGCAGTAGCTTCCCCTGGAGAAGAGGAAAAGCAAACCTTCATTGAGACCCAAGCGGTCTCTCCTGTGCTCTGTGACAATAATAAAGTTCCAGCCCTTGGCAAAAAAAAAA</dnaseq>
 
<dnaseq>AGCTCATATAAGGTAAAAGGCAGAGTGGCCTCTGTGGCCAGGGAGCCGCAGGCAAGGGACTAAGGGGAGGGGGGCTCAGTGCCAGCTGCTTAAAAATGCCCCTGTGGCAGCGAGGGGCACCAGAGGCTGGGTCTAATTAGTTGAGAAGCAGTGACACCCCCAACCACTCCCCAAACAGGCTGGCTCCCGTCTCCAGGCCCCAAGGAGCCACACCTGGACCAGACCCCAGGAAAGACATGTGGTGGAAACCCTACGGCCCTGGAGCCCAAGGAGAGCAACGGCTGTAACAAGTGCACAGGCAAGGGCGCTAGCTGGTGGGAGCCACCCCGCCATGCTGATGTCAGAGAAGCAAGAACTCTGGAGAAGCAGCCTCCTGGGACCAGAGGAGGGCCAGCAGCAGGCAGCCCGGAGACAGAACTCTCACCCACGCCTCTCCAAGCCTCCCAACAGAAGACAGAGGTCCCCCACAGCCAGAGACATTTCCTGAAGACATGGGGAACACAGAGGCAGAAACAGCCCATCCACCCAGGAGCTGTCCCCCACACTGCCGGGAGCCGGCACCCAGAGCCGCCAGGTAAAACTGAGGCCACCTGGTTCAACATCACCTTTCACAGAAGGGGAAGCAGCCACAGAAAGAAGGGCCTCGTTAAGAAGTGGAACCTGGGACCCCCAAGCGGTGTCTCTCATCCTGACTGGGGATCCAGAGTAGGAGGGAGCCTTTGGTGGGGTTGGAGTCCCGCCACAGGCCACCAGAGCGGAGCAGCGCAGCGCCCTGTCTCCCAGCCTGAGGTGCAGTGCTGCATCTCTGGTCAGTTGGGAGTCTGAGATGAAGCACTGTAGCTCAGGAAGAGAGAAGTTGTTCTGCAGCCATCAGCCTGGAAGTGCCTGGCTGGTGGGCCTTCTTGCAGTAGCTTCCCCTGGAGAAGAGGAAAAGCAAACCTTCATTGAGACCCAAGCGGTCTCTCCTGTGCTCTGTGACAATAATAAAGTTCCAGCCCTTGGCAAAAAAAAAA</dnaseq>

Latest revision as of 08:56, 28 June 2016

Annotated Information

Approved Symbol

CARMN

Approved Name

cardiac mesoderm enhancer-associated non-coding RNA

Previous Symbols

MIR143HG

Synonyms

CARMEN

Function

Identification of CARMEN. [1].

CARMEN is a crucial regulator of cardiac cell differentiation and homeostasis. CARMEN exhibits RNA-dependent enhancing activity and is upstream of the cardiac mesoderm-specifying gene regulatory network. Interestingly, CARMEN interacts with SUZ12 and EZH2, two components of the polycomb repressive complex 2 (PRC2). CARMEN is modulated in cardiac disease and required for maintaining a differentiated cardiac fate. Carmen was significantly upregulated post myocardial infarction, supporting a role for Carmen in adult heart homeostasis and cardiac remodeling.[1].


Disease

cardiac differentiation

Chromosome

5q32

RefSeq ID

NR_105059

OMIM ID

_

Ensembl ID

ENSG00000249669

Expression

Primer Forward primer Reverse primer
CARMEN1 5'-GATGGAAAAGATGGCAGAGG-3' 5'-TAGGTGTTGGCTGAGTGCAG-3'[1]
CARMEN2 5'-CCAACCACTCCCCAAACA-3' 5'-TTACAGCCGTTGCTCTCCTT-3'[1]
CARMEN3 5'-GAGTAGGAGGGAGCCTTTGG-3' 5'-TTTGCCTCCATCTCCTCTGT-3'[1]
Cloning 5'-TACCGAGCTCGGATCCtcagtgccagctgcttaaaa-3' 5'-CTGGACTAGTGGATCCaggcacagtgttagagtttgct-3'[1]

Reference

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Ounzain, S. et al. CARMEN, a human super enhancer-associated long noncoding RNA controlling cardiac specification, differentiation and homeostasis. J Mol Cell Cardiol 89, 98-112, doi:10.1016/j.yjmcc.2015.09.016 (2015).

Sequence

>gi|563318940|ref|NR_105059.1| Homo sapiens cardiac mesoderm enhancer-associated non-coding RNA (CARMN), transcript variant 1, long non-coding RNA

000001 AGCTCATATA AGGTAAAAGG CAGAGTGGCC TCTGTGGCCA GGGAGCCGCA GGCAAGGGAC TAAGGGGAGG GGGGCTCAGT 000080
000081 GCCAGCTGCT TAAAAATGCC CCTGTGGCAG CGAGGGGCAC CAGAGGCTGG GTCTAATTAG TTGAGAAGCA GTGACACCCC 000160
000161 CAACCACTCC CCAAACAGGC TGGCTCCCGT CTCCAGGCCC CAAGGAGCCA CACCTGGACC AGACCCCAGG AAAGACATGT 000240
000241 GGTGGAAACC CTACGGCCCT GGAGCCCAAG GAGAGCAACG GCTGTAACAA GTGCACAGGC AAGGGCGCTA GCTGGTGGGA 000320
000321 GCCACCCCGC CATGCTGATG TCAGAGAAGC AAGAACTCTG GAGAAGCAGC CTCCTGGGAC CAGAGGAGGG CCAGCAGCAG 000400
000401 GCAGCCCGGA GACAGAACTC TCACCCACGC CTCTCCAAGC CTCCCAACAG AAGACAGAGG TCCCCCACAG CCAGAGACAT 000480
000481 TTCCTGAAGA CATGGGGAAC ACAGAGGCAG AAACAGCCCA TCCACCCAGG AGCTGTCCCC CACACTGCCG GGAGCCGGCA 000560
000561 CCCAGAGCCG CCAGGTAAAA CTGAGGCCAC CTGGTTCAAC ATCACCTTTC ACAGAAGGGG AAGCAGCCAC AGAAAGAAGG 000640
000641 GCCTCGTTAA GAAGTGGAAC CTGGGACCCC CAAGCGGTGT CTCTCATCCT GACTGGGGAT CCAGAGTAGG AGGGAGCCTT 000720
000721 TGGTGGGGTT GGAGTCCCGC CACAGGCCAC CAGAGCGGAG CAGCGCAGCG CCCTGTCTCC CAGCCTGAGG TGCAGTGCTG 000800
000801 CATCTCTGGT CAGTTGGGAG TCTGAGATGA AGCACTGTAG CTCAGGAAGA GAGAAGTTGT TCTGCAGCCA TCAGCCTGGA 000880
000881 AGTGCCTGGC TGGTGGGCCT TCTTGCAGTA GCTTCCCCTG GAGAAGAGGA AAAGCAAACC TTCATTGAGA CCCAAGCGGT 000960
000961 CTCTCCTGTG CTCTGTGACA ATAATAAAGT TCCAGCCCTT GGCAAAAAAA AAA
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