Difference between revisions of "LRP1-AS"
Guangyu Wang (talk | contribs) (→pubmed IDs) |
Guangyu Wang (talk | contribs) (→Expression) |
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LRP1-AS | LRP1-AS | ||
===Approved Name=== | ===Approved Name=== | ||
| − | LRP1 antisense RNA | + | LRP1 antisense RNA <ref name="ref1" /> |
| + | |||
===Previous Symbols=== | ===Previous Symbols=== | ||
_ | _ | ||
===Synonyms=== | ===Synonyms=== | ||
_ | _ | ||
| + | ===Function=== | ||
| + | [[File: ABALON-2.jpg|right|thumb|400px|'''Proposed model of action of ''INXS'' lncRNA''' <ref name="ref1" />.]] | ||
| + | Lrp1-AS directly binds to High mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. Short oligonucleotides targeting Lrp1-AS inhibit the interaction of antisense transcript and Hmgb2 protein, and increase Lrp1 expression by enhancing Hmgb2 activity. LRP1-AS negatively regulates LRP1 gene expression through modulating the activity of the non-histone chromatin modifier HMGB2 and LRP1-AS is elevated in the brains of AD patients where it might repress LRP1 expression. <ref name="ref1" /> | ||
| + | |||
| + | ===Disease=== | ||
| + | *Alzheimer’s disease | ||
| + | ===Expression=== | ||
| + | Depending of the total amount of RNA present in a single cell it has been calculated that one copy of a transcript per cell corresponds to RPKM value between 0.5 and 5. Thus, in the human brain, there is approximately one transcript of LRP1-AS per cell. Decreased expression of LRP1 mRNA levels in AD subjects, while LRP1-AS levels were surprisingly increased. | ||
| + | {| class='wikitable' style="text-align:center" | ||
| + | |- | ||
| + | ! | Primer | ||
| + | ! | Forward primer | ||
| + | ! | Reverse primer | ||
| + | |- | ||
| + | | rowspan="1"|qRT-PCR | ||
| + | | | 5'-GGAAAGCTGCAGCCTTTCTT-3' | ||
| + | | | 5'-GTGGCTGTGTTGAAGGATAC-3'<ref name="ref1" /> | ||
| + | |} | ||
| + | |||
===Chromosome=== | ===Chromosome=== | ||
12q13.3 | 12q13.3 | ||
| Line 20: | Line 40: | ||
<ref name="ref1"> Yamanaka, Y. et al. Antisense RNA controls LRP1 Sense transcript expression through interaction with a chromatin-associated protein, HMGB2. Cell reports 11, 967-976, doi:10.1016/j.celrep.2015.04.011 (2015).</ref>(1) | <ref name="ref1"> Yamanaka, Y. et al. Antisense RNA controls LRP1 Sense transcript expression through interaction with a chromatin-associated protein, HMGB2. Cell reports 11, 967-976, doi:10.1016/j.celrep.2015.04.011 (2015).</ref>(1) | ||
</references> | </references> | ||
| + | |||
| + | |||
| + | [[Category: Antisense]] | ||
===Sequence=== | ===Sequence=== | ||
>gi|822092842|ref|NR_131939.1| Homo sapiens LRP1 antisense RNA (LRP1-AS), transcript variant 2, long non-coding RNA | >gi|822092842|ref|NR_131939.1| Homo sapiens LRP1 antisense RNA (LRP1-AS), transcript variant 2, long non-coding RNA | ||
<dnaseq>GTGCACCCCTGCTGCATGAGGCTCAGCCCCAGGGGTCCAACAGAGGTCACAGTCACAGCACAGAATGGAAGGCTTGCAGGGAGGGCTGCCCTGGAGGGCAGAGTGAAAGCAGGGGAAAGCTGCAGCCTTTCTTCTTCGTCCTCCTTGGGGAGCTAATTACAGGCCAGCAGCCACTGTGCAGGTGAGCTGTGGACAGCAGCAGCCCAAGGACGCTATCAGGGATGCTCTGGAGGAGCCAAAGGCAGAAATGTGAACCTTACGCTGAGGCCACGGAGAGTGAGGGCTCTCAGAGGAACCCCTGCTCGCCACCATCCCGGGGAGGGGCCTTTCTCTCTCCCAGAATGAATCCCTTGTAACCTCCTGGGATCTTTCTTTTCATCACAAGAACCAAATAAAGAAACTTGAAGATCTTCTAAAAAAAAAAA</dnaseq> | <dnaseq>GTGCACCCCTGCTGCATGAGGCTCAGCCCCAGGGGTCCAACAGAGGTCACAGTCACAGCACAGAATGGAAGGCTTGCAGGGAGGGCTGCCCTGGAGGGCAGAGTGAAAGCAGGGGAAAGCTGCAGCCTTTCTTCTTCGTCCTCCTTGGGGAGCTAATTACAGGCCAGCAGCCACTGTGCAGGTGAGCTGTGGACAGCAGCAGCCCAAGGACGCTATCAGGGATGCTCTGGAGGAGCCAAAGGCAGAAATGTGAACCTTACGCTGAGGCCACGGAGAGTGAGGGCTCTCAGAGGAACCCCTGCTCGCCACCATCCCGGGGAGGGGCCTTTCTCTCTCCCAGAATGAATCCCTTGTAACCTCCTGGGATCTTTCTTTTCATCACAAGAACCAAATAAAGAAACTTGAAGATCTTCTAAAAAAAAAAA</dnaseq> | ||
Latest revision as of 12:36, 28 June 2016
Contents
Annotated Information
Approved Symbol
LRP1-AS
Approved Name
LRP1 antisense RNA [1]
Previous Symbols
_
Synonyms
_
Function
Proposed model of action of INXS lncRNA [1].
Lrp1-AS directly binds to High mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. Short oligonucleotides targeting Lrp1-AS inhibit the interaction of antisense transcript and Hmgb2 protein, and increase Lrp1 expression by enhancing Hmgb2 activity. LRP1-AS negatively regulates LRP1 gene expression through modulating the activity of the non-histone chromatin modifier HMGB2 and LRP1-AS is elevated in the brains of AD patients where it might repress LRP1 expression. [1]
Disease
- Alzheimer’s disease
Expression
Depending of the total amount of RNA present in a single cell it has been calculated that one copy of a transcript per cell corresponds to RPKM value between 0.5 and 5. Thus, in the human brain, there is approximately one transcript of LRP1-AS per cell. Decreased expression of LRP1 mRNA levels in AD subjects, while LRP1-AS levels were surprisingly increased.
| Primer | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-GGAAAGCTGCAGCCTTTCTT-3' | 5'-GTGGCTGTGTTGAAGGATAC-3'[1] |
Chromosome
12q13.3
RefSeq ID
NR_131939
OMIM ID
_
Ensembl ID
ENSG00000259125
Reference
Sequence
>gi|822092842|ref|NR_131939.1| Homo sapiens LRP1 antisense RNA (LRP1-AS), transcript variant 2, long non-coding RNA
000001 GTGCACCCCT GCTGCATGAG GCTCAGCCCC AGGGGTCCAA CAGAGGTCAC AGTCACAGCA CAGAATGGAA GGCTTGCAGG 000080
000081 GAGGGCTGCC CTGGAGGGCA GAGTGAAAGC AGGGGAAAGC TGCAGCCTTT CTTCTTCGTC CTCCTTGGGG AGCTAATTAC 000160
000161 AGGCCAGCAG CCACTGTGCA GGTGAGCTGT GGACAGCAGC AGCCCAAGGA CGCTATCAGG GATGCTCTGG AGGAGCCAAA 000240
000241 GGCAGAAATG TGAACCTTAC GCTGAGGCCA CGGAGAGTGA GGGCTCTCAG AGGAACCCCT GCTCGCCACC ATCCCGGGGA 000320
000321 GGGGCCTTTC TCTCTCCCAG AATGAATCCC TTGTAACCTC CTGGGATCTT TCTTTTCATC ACAAGAACCA AATAAAGAAA 000400
000401 CTTGAAGATC TTCTAAAAAA AAAAA
000081 GAGGGCTGCC CTGGAGGGCA GAGTGAAAGC AGGGGAAAGC TGCAGCCTTT CTTCTTCGTC CTCCTTGGGG AGCTAATTAC 000160
000161 AGGCCAGCAG CCACTGTGCA GGTGAGCTGT GGACAGCAGC AGCCCAAGGA CGCTATCAGG GATGCTCTGG AGGAGCCAAA 000240
000241 GGCAGAAATG TGAACCTTAC GCTGAGGCCA CGGAGAGTGA GGGCTCTCAG AGGAACCCCT GCTCGCCACC ATCCCGGGGA 000320
000321 GGGGCCTTTC TCTCTCCCAG AATGAATCCC TTGTAACCTC CTGGGATCTT TCTTTTCATC ACAAGAACCA AATAAAGAAA 000400
000401 CTTGAAGATC TTCTAAAAAA AAAAA
