Difference between revisions of "IRAIN"
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===Synonyms=== | ===Synonyms=== | ||
IGF1R-AS | IGF1R-AS | ||
| − | [[File: The diagram of the IRAIN | + | [[File: The diagram of the IRAIN and IGF1R locus.png|right|thumb|400px|''' The diagram of the IRAIN and IGF1R locus'''<ref name="ref1" />]] |
===Chromosome=== | ===Chromosome=== | ||
15q26.3 | 15q26.3 | ||
===RefSeq ID=== | ===RefSeq ID=== | ||
NR_126453 | NR_126453 | ||
| − | |||
| − | |||
===Characteristics=== | ===Characteristics=== | ||
| − | IRAIN is a 5366 bp long non-coding RNA and its promoter is rich in CpG dinucleotides <ref name="ref1" />. | + | IRAIN is a 5366 bp long non-coding RNA and its promoter is rich in CpG dinucleotides <ref name="ref1" />. IRAIN is transcribed in an antisense direction of IGF1R from an intronic promoter <ref name="ref1" />. |
===Expression=== | ===Expression=== | ||
| − | + | IRAIN is downregulated in leukemia cell lines and blood of high-risk acute myeloid leukemia (AML) patients <ref name="ref1" />. | |
| − | |||
| − | |||
{| class='wikitable' style="text-align:center" | {| class='wikitable' style="text-align:center" | ||
|- | |- | ||
| Line 25: | Line 21: | ||
| rowspan="1"|Quantitative PCR | | rowspan="1"|Quantitative PCR | ||
| | 5'-CGACACATGGTCCAATCACTGTT- 3' <ref name="ref1" /> | | | 5'-CGACACATGGTCCAATCACTGTT- 3' <ref name="ref1" /> | ||
| − | | | | + | | | 5'-AGACTCCCCTAGGACTGCCATCT-3' <ref name="ref1" /> |
|} | |} | ||
===Regulation=== | ===Regulation=== | ||
| − | IRAIN lncRNA is imprinted | + | IRAIN lncRNA is imprinted, and it is expressed exclusively from the paternal allele, with the maternal counterpart being silenced <ref name="ref1" />. |
| − | |||
===Function=== | ===Function=== | ||
| − | IRAIN lncRNA may be actively involved in the interaction of two remote regions of the IGF1R gene <ref name="ref1" /> | + | IRAIN is an imprinted lncRNA that is involved in long-range DNA interactions <ref name="ref1" />. IRAIN interacts with chromatin DNA and is involved in the formation of an intrachromosomal enhancer/promoter loop. Knockdown of IRAIN lncRNA with shRNA abolishes this intrachromosomal interaction <ref name="ref1" />. IRAIN lncRNA may be actively involved in the interaction of two remote regions of the IGF1R gene <ref name="ref1" />. |
| + | |||
| + | ===Disease=== | ||
| + | * acute myeloid leukemia | ||
==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | * Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, Changchun, Jilin 130061, PR China Stanford University Medical School, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA. | + | * Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, Changchun, Jilin 130061, PR China Stanford University Medical School, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA.<ref name="ref1" /> |
| − | |||
==References== | ==References== | ||
| Line 43: | Line 40: | ||
</ref>(1) | </ref>(1) | ||
</references> | </references> | ||
| − | |||
| − | |||
===Sequence=== | ===Sequence=== | ||
>gi|704000384|ref|NR_126453.1| Homo sapiens IGF1R antisense imprinted non-protein coding RNA (IRAIN), long non-coding RNA | >gi|704000384|ref|NR_126453.1| Homo sapiens IGF1R antisense imprinted non-protein coding RNA (IRAIN), long non-coding RNA | ||
<dnaseq>AGCCTTTCTGAATTGCCCCGGTGATGGGGCCATACAACCTCCGCTGCCTCCTCCTTCCCCGCCTCACTCGTGAAGGCTCAGTCGTGATTTTTTCAAAGTTAATCGGCACCACCTGCATACCCTCGGCAACCATTACTCACCAACACGCCCTCACCGTTCCCAGTCCCAGTTCTGCGACATTTAAACTTTTAATCGTGTTCAAATTACCCTACCATCTTGAATGTACCAGTAAGCCACACTTTCAACTTTTTCAGAAGAATAAAACCCCCACCTCCTCCGGCCGTAGACACCGGGGGGAGTTCTCGCTCCCCGCATCCCCGCGCTTCCCTACTAACCCCCACCCGACACCACCAAAACAAAAGCTATCAGATTGCAAAAGACAGCGAATGTGGAGCGACACTGCTTATTAACTACGTACAAAGGAAGTGCGGGGCGGCAGAAGAGAGGGCGACACGACGCCGACCACAACAAAACCAAGGCCAGGGCTCCGCGCTCGCAAAACACAACACCCTCCCCGCGCCGCCGCAGACCCTGGCGCTGAAACCACAGCGTACGACACCTCTCGCCAGGGACGGGCGTCTAGGTGTCCCCGTGAGGCGGCGACTACACCCCGCGCTGCGAGCACGTGTGTCGGAATGCACGTCGGCTCCGACCACACACCCACCAACGCACACTCGGATGCGGCCCTAGTACCCCTCCATCCCCCGGCCCGGGAGAAACTGGGAGACCATCCCAGAGCCCTCTGAACTGGGGACCCAGTGGCGCTCCCCGGAAGAGACAAGCTGAGGAAGACTCCCGGCCTCGCACTTCCCGGGGGGCTGCCCAGAGAGCGCTTCCCCTCACCCCACCGCCCAGCCCCGGGACGCCGGGGGCAGGTAACCCAACGAGGAGCGGGCCAAAGTGACGGGCAAGGCCAGGAACCCGAGGAGCCCGAGGGGCAAAAGCACGTTGGGCGACGGGAGGCAGCGACGGGCGCTTGGAGACGTGTCCTGGGGCTTCGGGGCCTCTCCGCAGGGCACCTCCCCCCTCGAGGGCAACTCGCACCCCGCGGGCCGGGCGGCTGCCCGCTCCCCACTGCCCGGTCAGCCCCAGCAGCGGAGACGCGGTGCGGCCCAGGGTGCTGTCGCTGCTGCCTGCGCGCCGCAGGGGATCAGCGCTGTGTGCGGAGAGGCTCTTGCGCATGGAAGGGGCGAGGTGCGGGTCGCGGGGCCCGGGAACCGCGGAGCCCGCCCCGGCACCAACTTCCCTACCGAGTCCTCGCGCCTAGCCGGGAAACACGGCTGGGCTGTCAGGACGGAGAAAGAGAAAAGCCCTTCCCGCGCGGGGATGCGCGGAGCCAGGGCCCGCGGGGCTCCAGACGAACGAGCCCGGGTCCCGGGTCCGCTGCAGCCTCCCTCCCGGCAGAGCCGCCCTGCGCCCCACCCCGCCGCTCCCGAGCCCGGGGGCCCGACAGCTGCGACGGTGGCAACTCGGGTTTCGCAAACAGGGCGCAGCCCCTCGGAGGAAAAGTTCCCGCAGTGGCCGCGGGCGGCGGGCACATACTCACTTTCTCCACTCGTCGGCCAGAGCGAGAGCGCGGCGGAGAGAAACAGGAGCCCCCACAGCGAGGTCGGGGACCCTCCTCCGGAGCCAGACTTCATTCCTTTTATTTGGGATGAAATTCCCCTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAAAAGGAAATGAAAGGAAACAAGTCTCAAACTCAGTCTTCGCTCCCCCTCCAAAAACAAGGGCGAAGGAAACAATACTCCGAAGGCGGCGCCGCCGGGGCCCTCCGGGTGCGCGGACGGGGCGGGCGGCGCGGGGCGGCCCCTCAGCGCCGGCAGCAGCGGCCCAGGGCTCGGCGGAGTCGGGAACCCGAGGCGCGCCGGGGCGGGCTGTCGGCGTCGTCGGCCTCCATTTTCAAACCCGGAGAGGCAGGAAGGGGGGAAAACTGCAAAGAAAGGGGGCAAAGCCCAAATCTGCCTAGGCGAGGAAAAACAAGCCCGGCCCTGGCCGCGCGGGAGGCGGCCGGACGCCCGAGCTTCCGCCGCGGGAGGGCGCGACGCAGTTCGCAAGATCGCCCCGAAGTCCGGGTCACAGGCGAGGCCGGCGAGGGGCAGAAACGCGGAGTCAAAATGAATGAGCGGCTCCCCCTCCTCCTCCTCCTCCTGGCTCCGCTCGCCGCCGCCTCCTCCCTCAGCGCCGCCGCCGCCGCGGCGAGCTCCTTCCCAAATCCAGGACACACACAAAGCGGCGGGCCGGCCGCGCCGCGCTCAGCACTCCCGCCGCGCCGCCCGGCTCTGCGCCGCCGCCCGCCCCGCGCCGGCCCCCGCCCGGCCCCCGCCCGCCGCCGCCTGCTCTGCGCGGGGGCTCCGGCTCGCTGAAGGTCACAGCCGAGGCGAGGCGCGCCGTGGAGCTGCCCCCGCGCGGGAGGGGCGTGGAGGGCGCGGGGGGATCCGGGGAGCGGGGCCGAGGGTCTGCGAGCGCCGGCCGTGGGCAGGCTCGCTGGCGGCGTCCCTCTCGCTCTCCCGCCTCTCTCGAGTTCGCCTGGTGCGCTCGCTCCTCCTCGGGTTTTTTCCCTTTTTTTTTCCGCTCAGCGGAGTTAATGCTGGTAAACAAGAGCCCCAGCCTCGCCGCGCCGCCGCCGCCGCTGCCACACACTGGGGGCTCGCGCGCGCGCGCGCGCCCTGGCCCGCGCACACGCGCCCGCTCGCACACTCGAGCGGCCACCGCCGCAGCAGCGGCGCGCCGAGGGCCCGGCCCAGGCCGGCGCGCGCAAAAAACCGGAGCCCGGTTCCCCAAGACGTGCGGAGCGGAGCCCGGGCGTGGGGCGCGAGACTGCGAGGCGCCGCCCCCATCTCCCGCCCCACCGCCTCCCGCGCGCACACAGAGACACGCGCGCGTGCCCACCCGCTCCCGCCCCCACCGCACTGCGCCGGCCACCCCGCACGCGCCCTCCCCTCCCGCCCGACTCCAAAAACAATGCCCCCGCCGCCGGTCCGGTCTCGGCGGGGCAGGGGTGGGTAGCCAGGGAAAGGCTAGGCAGGGGAGGGGTGGGCAGGAGAGGGGAGGGGTGGGCGGGGAGTGCGGGGCTGGGAGAGGTTCATTGAAAACAACAACAGCGCGGCTGGAAAGCGCGTTTCCCCGGTTCTTGAAAAGCTTCATTGTTTCTTGCAGCTGTTGCAAAATAAATAAATGCGTGCATGCGGCATTTTTTTCGCTGCGTACTGGAAAGCCCGAACGTGGCGGAAGGGGCGCACCTGGGAGAAAGGGGACCCCCCCCCCAGGCTACTACGCCGGGCACTTTCACGCCAGGCTGGGGTGCCCGCGGCGTTGCGACCGTGGAGCCCGGAGTTTGGGGGCTCAGGGGGAGCGGAGGGGGAAGGGCGCGGCCCGTGTGGCTCGGCCCAGAGTGGCTCGAGGAGAGAGGACCCCGCCGCCCCTCGCGGGCCCCGCGCCCCGGAAGGGGGGCCCAGACAGGCAGCGCCGCGTGCCCCGGGCGCGTCCCCGCGGCGGCCGCCAGAGGGCGCGGCGGGCGCTGGGGAGGGCAGCCCGCGGAGAGTCGCCTCCCTCCCTCCGGGGGAGGAAATGTGGGGGCCCGGGCTGGGGGCCGACTCGGCTGGGTCGCGCTGGCGTGGCCTCCCGGACCAGCCGCCCGCCCTGAGCGCTCTCCGCGGCCTCGCGCAGCTCAGGGCTCCACCACGTGGGCGGCTGCGGGGAGCGGCCGGGGCGCGTGCACGCTGCGGGTCTCCGAAGCTCGGGGGGCGTGTCCGGGTCCCGGGGCGCCGAGGAGGGGGTGGAGGCTGGGGCCGGGAAGCCTCCCTTGGCCGCGGTCCCGACCCGGGAACACGCCGCATGCACTCGGGATCAGCGGCTACTGCGGAAACCCACCCGCACCTGTCTCGGGTCCTCCCAGGCACGTTCCTCTTGCTTCGTTTGCTACGTAAGTGACTGGAAACCAGTACCGACCCTAAGTCTCCGCGGTTGTTTTCTTGAATGTGCAGGAGGAGAGGTTTCCCCAAAGCGAGACTCCCCTAGGACTGCCATCTAATGAATAAATGCTGCGGAGACCAGGTCCTCAAAGAGGGAAACAGCCCAGCAGTGGCAGGAGAGAGGAGTTTGAAATACGTGGACTTTTACAAACAGTGATTGGACCATGTGTCGCCCTTAATGTGGTCCGGTTTCATGACTCAGGCTAATTGATGCCAGTAATAAATTTTTTTAAGATAGCTAATAAAAGAGTTGTGAATTGTCGAGATAAGAAGTGGTTGTCTCAAACAATCGGGTAGGATGGTAGAAGGGACCTGATGTAAAGGAAGGTAGTAGGTTTCTTTTACTCTCTAATTCTAAAATACTTCTGCATTAGAACGTGTATCCATCGTTCGTAAATAATTTTTTAAGAGCTGAAAACAAGTCAAAAGCTATCATAACGGAAACTTAGCCCTTTTTTTCTAAAGTTGTTTCCTAGATCATTTTCCTCCTTGGAAATTTCTAGCTCAATTCAGAGTCAGTTGTATAGAAAGAAAATAAACCTTGTTTTACCTAAGAAAGTTATTCATATGATGGGATGGTTTTGTTTAACTGAAATTCAGCTTGATTTTTAAATAAAGAGTTTCAGTTTTGGATGTTCCTAGATTTAACAGTGAAATAAATTTATTAGAATCTTAAAATGTATTATTTAAAAATTAGGCACTACACATGAAGAATTAAAGTCCAGTCTACTAATTTTTAAATATTTCTTAAGTTCTCTGCATGAGGTTGGTCTTGATTCCTGCAGATCCACTCTGGAGTTAAATGCAAAGGAATATTGATATATAATTTCATTATACTTGATTTCAGAATTTTTAATACATACTTGTAAGACAAAAATTTAGAGCTGATTTTTAAAAGGCTAGTGCTAATATAAAATCTTCAAATACTACCCAATGTATTTTTAGTATTAGTAATAAATATGCCTGTTTATTTGTCCCCAAGAACATAAAGAGGTCTATAGAATTTTTAAATGTATATAGGTTGTTGTAGGGGTTGTAGGATTTGTTTTTGTGTGTTTTTTTTTTACTAAAATGATTTTTATTTGTGAATTTTTTAAATGTTTGTGTTTTCCTGAAGTAGTAGGAAATCAATCGTATTAAGTTATTTTAAACAGTGAAGTGGGAGATGTATCATTATATAGCTTAATATTTAGGGAAAAAAAAGGAACAAAAATTTAGGTTGTCTTTACGCTCTAACTTGACCACATGGACAAAGGAGGTGATTTGTATTTCTTCAATGATTATATTTTACATACACAAGTCAAACTTATTTTTTCAACCTTGAAATAAAATCATGTAGACAAAAA</dnaseq> | <dnaseq>AGCCTTTCTGAATTGCCCCGGTGATGGGGCCATACAACCTCCGCTGCCTCCTCCTTCCCCGCCTCACTCGTGAAGGCTCAGTCGTGATTTTTTCAAAGTTAATCGGCACCACCTGCATACCCTCGGCAACCATTACTCACCAACACGCCCTCACCGTTCCCAGTCCCAGTTCTGCGACATTTAAACTTTTAATCGTGTTCAAATTACCCTACCATCTTGAATGTACCAGTAAGCCACACTTTCAACTTTTTCAGAAGAATAAAACCCCCACCTCCTCCGGCCGTAGACACCGGGGGGAGTTCTCGCTCCCCGCATCCCCGCGCTTCCCTACTAACCCCCACCCGACACCACCAAAACAAAAGCTATCAGATTGCAAAAGACAGCGAATGTGGAGCGACACTGCTTATTAACTACGTACAAAGGAAGTGCGGGGCGGCAGAAGAGAGGGCGACACGACGCCGACCACAACAAAACCAAGGCCAGGGCTCCGCGCTCGCAAAACACAACACCCTCCCCGCGCCGCCGCAGACCCTGGCGCTGAAACCACAGCGTACGACACCTCTCGCCAGGGACGGGCGTCTAGGTGTCCCCGTGAGGCGGCGACTACACCCCGCGCTGCGAGCACGTGTGTCGGAATGCACGTCGGCTCCGACCACACACCCACCAACGCACACTCGGATGCGGCCCTAGTACCCCTCCATCCCCCGGCCCGGGAGAAACTGGGAGACCATCCCAGAGCCCTCTGAACTGGGGACCCAGTGGCGCTCCCCGGAAGAGACAAGCTGAGGAAGACTCCCGGCCTCGCACTTCCCGGGGGGCTGCCCAGAGAGCGCTTCCCCTCACCCCACCGCCCAGCCCCGGGACGCCGGGGGCAGGTAACCCAACGAGGAGCGGGCCAAAGTGACGGGCAAGGCCAGGAACCCGAGGAGCCCGAGGGGCAAAAGCACGTTGGGCGACGGGAGGCAGCGACGGGCGCTTGGAGACGTGTCCTGGGGCTTCGGGGCCTCTCCGCAGGGCACCTCCCCCCTCGAGGGCAACTCGCACCCCGCGGGCCGGGCGGCTGCCCGCTCCCCACTGCCCGGTCAGCCCCAGCAGCGGAGACGCGGTGCGGCCCAGGGTGCTGTCGCTGCTGCCTGCGCGCCGCAGGGGATCAGCGCTGTGTGCGGAGAGGCTCTTGCGCATGGAAGGGGCGAGGTGCGGGTCGCGGGGCCCGGGAACCGCGGAGCCCGCCCCGGCACCAACTTCCCTACCGAGTCCTCGCGCCTAGCCGGGAAACACGGCTGGGCTGTCAGGACGGAGAAAGAGAAAAGCCCTTCCCGCGCGGGGATGCGCGGAGCCAGGGCCCGCGGGGCTCCAGACGAACGAGCCCGGGTCCCGGGTCCGCTGCAGCCTCCCTCCCGGCAGAGCCGCCCTGCGCCCCACCCCGCCGCTCCCGAGCCCGGGGGCCCGACAGCTGCGACGGTGGCAACTCGGGTTTCGCAAACAGGGCGCAGCCCCTCGGAGGAAAAGTTCCCGCAGTGGCCGCGGGCGGCGGGCACATACTCACTTTCTCCACTCGTCGGCCAGAGCGAGAGCGCGGCGGAGAGAAACAGGAGCCCCCACAGCGAGGTCGGGGACCCTCCTCCGGAGCCAGACTTCATTCCTTTTATTTGGGATGAAATTCCCCTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAAAAGGAAATGAAAGGAAACAAGTCTCAAACTCAGTCTTCGCTCCCCCTCCAAAAACAAGGGCGAAGGAAACAATACTCCGAAGGCGGCGCCGCCGGGGCCCTCCGGGTGCGCGGACGGGGCGGGCGGCGCGGGGCGGCCCCTCAGCGCCGGCAGCAGCGGCCCAGGGCTCGGCGGAGTCGGGAACCCGAGGCGCGCCGGGGCGGGCTGTCGGCGTCGTCGGCCTCCATTTTCAAACCCGGAGAGGCAGGAAGGGGGGAAAACTGCAAAGAAAGGGGGCAAAGCCCAAATCTGCCTAGGCGAGGAAAAACAAGCCCGGCCCTGGCCGCGCGGGAGGCGGCCGGACGCCCGAGCTTCCGCCGCGGGAGGGCGCGACGCAGTTCGCAAGATCGCCCCGAAGTCCGGGTCACAGGCGAGGCCGGCGAGGGGCAGAAACGCGGAGTCAAAATGAATGAGCGGCTCCCCCTCCTCCTCCTCCTCCTGGCTCCGCTCGCCGCCGCCTCCTCCCTCAGCGCCGCCGCCGCCGCGGCGAGCTCCTTCCCAAATCCAGGACACACACAAAGCGGCGGGCCGGCCGCGCCGCGCTCAGCACTCCCGCCGCGCCGCCCGGCTCTGCGCCGCCGCCCGCCCCGCGCCGGCCCCCGCCCGGCCCCCGCCCGCCGCCGCCTGCTCTGCGCGGGGGCTCCGGCTCGCTGAAGGTCACAGCCGAGGCGAGGCGCGCCGTGGAGCTGCCCCCGCGCGGGAGGGGCGTGGAGGGCGCGGGGGGATCCGGGGAGCGGGGCCGAGGGTCTGCGAGCGCCGGCCGTGGGCAGGCTCGCTGGCGGCGTCCCTCTCGCTCTCCCGCCTCTCTCGAGTTCGCCTGGTGCGCTCGCTCCTCCTCGGGTTTTTTCCCTTTTTTTTTCCGCTCAGCGGAGTTAATGCTGGTAAACAAGAGCCCCAGCCTCGCCGCGCCGCCGCCGCCGCTGCCACACACTGGGGGCTCGCGCGCGCGCGCGCGCCCTGGCCCGCGCACACGCGCCCGCTCGCACACTCGAGCGGCCACCGCCGCAGCAGCGGCGCGCCGAGGGCCCGGCCCAGGCCGGCGCGCGCAAAAAACCGGAGCCCGGTTCCCCAAGACGTGCGGAGCGGAGCCCGGGCGTGGGGCGCGAGACTGCGAGGCGCCGCCCCCATCTCCCGCCCCACCGCCTCCCGCGCGCACACAGAGACACGCGCGCGTGCCCACCCGCTCCCGCCCCCACCGCACTGCGCCGGCCACCCCGCACGCGCCCTCCCCTCCCGCCCGACTCCAAAAACAATGCCCCCGCCGCCGGTCCGGTCTCGGCGGGGCAGGGGTGGGTAGCCAGGGAAAGGCTAGGCAGGGGAGGGGTGGGCAGGAGAGGGGAGGGGTGGGCGGGGAGTGCGGGGCTGGGAGAGGTTCATTGAAAACAACAACAGCGCGGCTGGAAAGCGCGTTTCCCCGGTTCTTGAAAAGCTTCATTGTTTCTTGCAGCTGTTGCAAAATAAATAAATGCGTGCATGCGGCATTTTTTTCGCTGCGTACTGGAAAGCCCGAACGTGGCGGAAGGGGCGCACCTGGGAGAAAGGGGACCCCCCCCCCAGGCTACTACGCCGGGCACTTTCACGCCAGGCTGGGGTGCCCGCGGCGTTGCGACCGTGGAGCCCGGAGTTTGGGGGCTCAGGGGGAGCGGAGGGGGAAGGGCGCGGCCCGTGTGGCTCGGCCCAGAGTGGCTCGAGGAGAGAGGACCCCGCCGCCCCTCGCGGGCCCCGCGCCCCGGAAGGGGGGCCCAGACAGGCAGCGCCGCGTGCCCCGGGCGCGTCCCCGCGGCGGCCGCCAGAGGGCGCGGCGGGCGCTGGGGAGGGCAGCCCGCGGAGAGTCGCCTCCCTCCCTCCGGGGGAGGAAATGTGGGGGCCCGGGCTGGGGGCCGACTCGGCTGGGTCGCGCTGGCGTGGCCTCCCGGACCAGCCGCCCGCCCTGAGCGCTCTCCGCGGCCTCGCGCAGCTCAGGGCTCCACCACGTGGGCGGCTGCGGGGAGCGGCCGGGGCGCGTGCACGCTGCGGGTCTCCGAAGCTCGGGGGGCGTGTCCGGGTCCCGGGGCGCCGAGGAGGGGGTGGAGGCTGGGGCCGGGAAGCCTCCCTTGGCCGCGGTCCCGACCCGGGAACACGCCGCATGCACTCGGGATCAGCGGCTACTGCGGAAACCCACCCGCACCTGTCTCGGGTCCTCCCAGGCACGTTCCTCTTGCTTCGTTTGCTACGTAAGTGACTGGAAACCAGTACCGACCCTAAGTCTCCGCGGTTGTTTTCTTGAATGTGCAGGAGGAGAGGTTTCCCCAAAGCGAGACTCCCCTAGGACTGCCATCTAATGAATAAATGCTGCGGAGACCAGGTCCTCAAAGAGGGAAACAGCCCAGCAGTGGCAGGAGAGAGGAGTTTGAAATACGTGGACTTTTACAAACAGTGATTGGACCATGTGTCGCCCTTAATGTGGTCCGGTTTCATGACTCAGGCTAATTGATGCCAGTAATAAATTTTTTTAAGATAGCTAATAAAAGAGTTGTGAATTGTCGAGATAAGAAGTGGTTGTCTCAAACAATCGGGTAGGATGGTAGAAGGGACCTGATGTAAAGGAAGGTAGTAGGTTTCTTTTACTCTCTAATTCTAAAATACTTCTGCATTAGAACGTGTATCCATCGTTCGTAAATAATTTTTTAAGAGCTGAAAACAAGTCAAAAGCTATCATAACGGAAACTTAGCCCTTTTTTTCTAAAGTTGTTTCCTAGATCATTTTCCTCCTTGGAAATTTCTAGCTCAATTCAGAGTCAGTTGTATAGAAAGAAAATAAACCTTGTTTTACCTAAGAAAGTTATTCATATGATGGGATGGTTTTGTTTAACTGAAATTCAGCTTGATTTTTAAATAAAGAGTTTCAGTTTTGGATGTTCCTAGATTTAACAGTGAAATAAATTTATTAGAATCTTAAAATGTATTATTTAAAAATTAGGCACTACACATGAAGAATTAAAGTCCAGTCTACTAATTTTTAAATATTTCTTAAGTTCTCTGCATGAGGTTGGTCTTGATTCCTGCAGATCCACTCTGGAGTTAAATGCAAAGGAATATTGATATATAATTTCATTATACTTGATTTCAGAATTTTTAATACATACTTGTAAGACAAAAATTTAGAGCTGATTTTTAAAAGGCTAGTGCTAATATAAAATCTTCAAATACTACCCAATGTATTTTTAGTATTAGTAATAAATATGCCTGTTTATTTGTCCCCAAGAACATAAAGAGGTCTATAGAATTTTTAAATGTATATAGGTTGTTGTAGGGGTTGTAGGATTTGTTTTTGTGTGTTTTTTTTTTACTAAAATGATTTTTATTTGTGAATTTTTTAAATGTTTGTGTTTTCCTGAAGTAGTAGGAAATCAATCGTATTAAGTTATTTTAAACAGTGAAGTGGGAGATGTATCATTATATAGCTTAATATTTAGGGAAAAAAAAGGAACAAAAATTTAGGTTGTCTTTACGCTCTAACTTGACCACATGGACAAAGGAGGTGATTTGTATTTCTTCAATGATTATATTTTACATACACAAGTCAAACTTATTTTTTCAACCTTGAAATAAAATCATGTAGACAAAAA</dnaseq> | ||
Latest revision as of 09:10, 7 December 2017
Contents
Annotated Information
Approved Symbol
IRAIN (IGF1R antisense imprinted non-protein coding RNA)
Synonyms
IGF1R-AS
The diagram of the IRAIN and IGF1R locus[1]
Chromosome
15q26.3
RefSeq ID
NR_126453
Characteristics
IRAIN is a 5366 bp long non-coding RNA and its promoter is rich in CpG dinucleotides [1]. IRAIN is transcribed in an antisense direction of IGF1R from an intronic promoter [1].
Expression
IRAIN is downregulated in leukemia cell lines and blood of high-risk acute myeloid leukemia (AML) patients [1].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| Quantitative PCR | 5'-CGACACATGGTCCAATCACTGTT- 3' [1] | 5'-AGACTCCCCTAGGACTGCCATCT-3' [1] |
Regulation
IRAIN lncRNA is imprinted, and it is expressed exclusively from the paternal allele, with the maternal counterpart being silenced [1].
Function
IRAIN is an imprinted lncRNA that is involved in long-range DNA interactions [1]. IRAIN interacts with chromatin DNA and is involved in the formation of an intrachromosomal enhancer/promoter loop. Knockdown of IRAIN lncRNA with shRNA abolishes this intrachromosomal interaction [1]. IRAIN lncRNA may be actively involved in the interaction of two remote regions of the IGF1R gene [1].
Disease
- acute myeloid leukemia
Labs working on this lncRNA
- Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, Changchun, Jilin 130061, PR China Stanford University Medical School, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA.[1]
References
Sequence
>gi|704000384|ref|NR_126453.1| Homo sapiens IGF1R antisense imprinted non-protein coding RNA (IRAIN), long non-coding RNA
000001 AGCCTTTCTG AATTGCCCCG GTGATGGGGC CATACAACCT CCGCTGCCTC CTCCTTCCCC GCCTCACTCG TGAAGGCTCA 000080
000081 GTCGTGATTT TTTCAAAGTT AATCGGCACC ACCTGCATAC CCTCGGCAAC CATTACTCAC CAACACGCCC TCACCGTTCC 000160
000161 CAGTCCCAGT TCTGCGACAT TTAAACTTTT AATCGTGTTC AAATTACCCT ACCATCTTGA ATGTACCAGT AAGCCACACT 000240
000241 TTCAACTTTT TCAGAAGAAT AAAACCCCCA CCTCCTCCGG CCGTAGACAC CGGGGGGAGT TCTCGCTCCC CGCATCCCCG 000320
000321 CGCTTCCCTA CTAACCCCCA CCCGACACCA CCAAAACAAA AGCTATCAGA TTGCAAAAGA CAGCGAATGT GGAGCGACAC 000400
000401 TGCTTATTAA CTACGTACAA AGGAAGTGCG GGGCGGCAGA AGAGAGGGCG ACACGACGCC GACCACAACA AAACCAAGGC 000480
000481 CAGGGCTCCG CGCTCGCAAA ACACAACACC CTCCCCGCGC CGCCGCAGAC CCTGGCGCTG AAACCACAGC GTACGACACC 000560
000561 TCTCGCCAGG GACGGGCGTC TAGGTGTCCC CGTGAGGCGG CGACTACACC CCGCGCTGCG AGCACGTGTG TCGGAATGCA 000640
000641 CGTCGGCTCC GACCACACAC CCACCAACGC ACACTCGGAT GCGGCCCTAG TACCCCTCCA TCCCCCGGCC CGGGAGAAAC 000720
000721 TGGGAGACCA TCCCAGAGCC CTCTGAACTG GGGACCCAGT GGCGCTCCCC GGAAGAGACA AGCTGAGGAA GACTCCCGGC 000800
000801 CTCGCACTTC CCGGGGGGCT GCCCAGAGAG CGCTTCCCCT CACCCCACCG CCCAGCCCCG GGACGCCGGG GGCAGGTAAC 000880
000881 CCAACGAGGA GCGGGCCAAA GTGACGGGCA AGGCCAGGAA CCCGAGGAGC CCGAGGGGCA AAAGCACGTT GGGCGACGGG 000960
000961 AGGCAGCGAC GGGCGCTTGG AGACGTGTCC TGGGGCTTCG GGGCCTCTCC GCAGGGCACC TCCCCCCTCG AGGGCAACTC 001040
001041 GCACCCCGCG GGCCGGGCGG CTGCCCGCTC CCCACTGCCC GGTCAGCCCC AGCAGCGGAG ACGCGGTGCG GCCCAGGGTG 001120
001121 CTGTCGCTGC TGCCTGCGCG CCGCAGGGGA TCAGCGCTGT GTGCGGAGAG GCTCTTGCGC ATGGAAGGGG CGAGGTGCGG 001200
001201 GTCGCGGGGC CCGGGAACCG CGGAGCCCGC CCCGGCACCA ACTTCCCTAC CGAGTCCTCG CGCCTAGCCG GGAAACACGG 001280
001281 CTGGGCTGTC AGGACGGAGA AAGAGAAAAG CCCTTCCCGC GCGGGGATGC GCGGAGCCAG GGCCCGCGGG GCTCCAGACG 001360
001361 AACGAGCCCG GGTCCCGGGT CCGCTGCAGC CTCCCTCCCG GCAGAGCCGC CCTGCGCCCC ACCCCGCCGC TCCCGAGCCC 001440
001441 GGGGGCCCGA CAGCTGCGAC GGTGGCAACT CGGGTTTCGC AAACAGGGCG CAGCCCCTCG GAGGAAAAGT TCCCGCAGTG 001520
001521 GCCGCGGGCG GCGGGCACAT ACTCACTTTC TCCACTCGTC GGCCAGAGCG AGAGCGCGGC GGAGAGAAAC AGGAGCCCCC 001600
001601 ACAGCGAGGT CGGGGACCCT CCTCCGGAGC CAGACTTCAT TCCTTTTATT TGGGATGAAA TTCCCCTTTC TCAAAAAAAA 001680
001681 AAAAAAAAAA AAAAAAGAAA AGAAAAGAAA AAAAGGAAAT GAAAGGAAAC AAGTCTCAAA CTCAGTCTTC GCTCCCCCTC 001760
001761 CAAAAACAAG GGCGAAGGAA ACAATACTCC GAAGGCGGCG CCGCCGGGGC CCTCCGGGTG CGCGGACGGG GCGGGCGGCG 001840
001841 CGGGGCGGCC CCTCAGCGCC GGCAGCAGCG GCCCAGGGCT CGGCGGAGTC GGGAACCCGA GGCGCGCCGG GGCGGGCTGT 001920
001921 CGGCGTCGTC GGCCTCCATT TTCAAACCCG GAGAGGCAGG AAGGGGGGAA AACTGCAAAG AAAGGGGGCA AAGCCCAAAT 002000
002001 CTGCCTAGGC GAGGAAAAAC AAGCCCGGCC CTGGCCGCGC GGGAGGCGGC CGGACGCCCG AGCTTCCGCC GCGGGAGGGC 002080
002081 GCGACGCAGT TCGCAAGATC GCCCCGAAGT CCGGGTCACA GGCGAGGCCG GCGAGGGGCA GAAACGCGGA GTCAAAATGA 002160
002161 ATGAGCGGCT CCCCCTCCTC CTCCTCCTCC TGGCTCCGCT CGCCGCCGCC TCCTCCCTCA GCGCCGCCGC CGCCGCGGCG 002240
002241 AGCTCCTTCC CAAATCCAGG ACACACACAA AGCGGCGGGC CGGCCGCGCC GCGCTCAGCA CTCCCGCCGC GCCGCCCGGC 002320
002321 TCTGCGCCGC CGCCCGCCCC GCGCCGGCCC CCGCCCGGCC CCCGCCCGCC GCCGCCTGCT CTGCGCGGGG GCTCCGGCTC 002400
002401 GCTGAAGGTC ACAGCCGAGG CGAGGCGCGC CGTGGAGCTG CCCCCGCGCG GGAGGGGCGT GGAGGGCGCG GGGGGATCCG 002480
002481 GGGAGCGGGG CCGAGGGTCT GCGAGCGCCG GCCGTGGGCA GGCTCGCTGG CGGCGTCCCT CTCGCTCTCC CGCCTCTCTC 002560
002561 GAGTTCGCCT GGTGCGCTCG CTCCTCCTCG GGTTTTTTCC CTTTTTTTTT CCGCTCAGCG GAGTTAATGC TGGTAAACAA 002640
002641 GAGCCCCAGC CTCGCCGCGC CGCCGCCGCC GCTGCCACAC ACTGGGGGCT CGCGCGCGCG CGCGCGCCCT GGCCCGCGCA 002720
002721 CACGCGCCCG CTCGCACACT CGAGCGGCCA CCGCCGCAGC AGCGGCGCGC CGAGGGCCCG GCCCAGGCCG GCGCGCGCAA 002800
002801 AAAACCGGAG CCCGGTTCCC CAAGACGTGC GGAGCGGAGC CCGGGCGTGG GGCGCGAGAC TGCGAGGCGC CGCCCCCATC 002880
002881 TCCCGCCCCA CCGCCTCCCG CGCGCACACA GAGACACGCG CGCGTGCCCA CCCGCTCCCG CCCCCACCGC ACTGCGCCGG 002960
002961 CCACCCCGCA CGCGCCCTCC CCTCCCGCCC GACTCCAAAA ACAATGCCCC CGCCGCCGGT CCGGTCTCGG CGGGGCAGGG 003040
003041 GTGGGTAGCC AGGGAAAGGC TAGGCAGGGG AGGGGTGGGC AGGAGAGGGG AGGGGTGGGC GGGGAGTGCG GGGCTGGGAG 003120
003121 AGGTTCATTG AAAACAACAA CAGCGCGGCT GGAAAGCGCG TTTCCCCGGT TCTTGAAAAG CTTCATTGTT TCTTGCAGCT 003200
003201 GTTGCAAAAT AAATAAATGC GTGCATGCGG CATTTTTTTC GCTGCGTACT GGAAAGCCCG AACGTGGCGG AAGGGGCGCA 003280
003281 CCTGGGAGAA AGGGGACCCC CCCCCCAGGC TACTACGCCG GGCACTTTCA CGCCAGGCTG GGGTGCCCGC GGCGTTGCGA 003360
003361 CCGTGGAGCC CGGAGTTTGG GGGCTCAGGG GGAGCGGAGG GGGAAGGGCG CGGCCCGTGT GGCTCGGCCC AGAGTGGCTC 003440
003441 GAGGAGAGAG GACCCCGCCG CCCCTCGCGG GCCCCGCGCC CCGGAAGGGG GGCCCAGACA GGCAGCGCCG CGTGCCCCGG 003520
003521 GCGCGTCCCC GCGGCGGCCG CCAGAGGGCG CGGCGGGCGC TGGGGAGGGC AGCCCGCGGA GAGTCGCCTC CCTCCCTCCG 003600
003601 GGGGAGGAAA TGTGGGGGCC CGGGCTGGGG GCCGACTCGG CTGGGTCGCG CTGGCGTGGC CTCCCGGACC AGCCGCCCGC 003680
003681 CCTGAGCGCT CTCCGCGGCC TCGCGCAGCT CAGGGCTCCA CCACGTGGGC GGCTGCGGGG AGCGGCCGGG GCGCGTGCAC 003760
003761 GCTGCGGGTC TCCGAAGCTC GGGGGGCGTG TCCGGGTCCC GGGGCGCCGA GGAGGGGGTG GAGGCTGGGG CCGGGAAGCC 003840
003841 TCCCTTGGCC GCGGTCCCGA CCCGGGAACA CGCCGCATGC ACTCGGGATC AGCGGCTACT GCGGAAACCC ACCCGCACCT 003920
003921 GTCTCGGGTC CTCCCAGGCA CGTTCCTCTT GCTTCGTTTG CTACGTAAGT GACTGGAAAC CAGTACCGAC CCTAAGTCTC 004000
004001 CGCGGTTGTT TTCTTGAATG TGCAGGAGGA GAGGTTTCCC CAAAGCGAGA CTCCCCTAGG ACTGCCATCT AATGAATAAA 004080
004081 TGCTGCGGAG ACCAGGTCCT CAAAGAGGGA AACAGCCCAG CAGTGGCAGG AGAGAGGAGT TTGAAATACG TGGACTTTTA 004160
004161 CAAACAGTGA TTGGACCATG TGTCGCCCTT AATGTGGTCC GGTTTCATGA CTCAGGCTAA TTGATGCCAG TAATAAATTT 004240
004241 TTTTAAGATA GCTAATAAAA GAGTTGTGAA TTGTCGAGAT AAGAAGTGGT TGTCTCAAAC AATCGGGTAG GATGGTAGAA 004320
004321 GGGACCTGAT GTAAAGGAAG GTAGTAGGTT TCTTTTACTC TCTAATTCTA AAATACTTCT GCATTAGAAC GTGTATCCAT 004400
004401 CGTTCGTAAA TAATTTTTTA AGAGCTGAAA ACAAGTCAAA AGCTATCATA ACGGAAACTT AGCCCTTTTT TTCTAAAGTT 004480
004481 GTTTCCTAGA TCATTTTCCT CCTTGGAAAT TTCTAGCTCA ATTCAGAGTC AGTTGTATAG AAAGAAAATA AACCTTGTTT 004560
004561 TACCTAAGAA AGTTATTCAT ATGATGGGAT GGTTTTGTTT AACTGAAATT CAGCTTGATT TTTAAATAAA GAGTTTCAGT 004640
004641 TTTGGATGTT CCTAGATTTA ACAGTGAAAT AAATTTATTA GAATCTTAAA ATGTATTATT TAAAAATTAG GCACTACACA 004720
004721 TGAAGAATTA AAGTCCAGTC TACTAATTTT TAAATATTTC TTAAGTTCTC TGCATGAGGT TGGTCTTGAT TCCTGCAGAT 004800
004801 CCACTCTGGA GTTAAATGCA AAGGAATATT GATATATAAT TTCATTATAC TTGATTTCAG AATTTTTAAT ACATACTTGT 004880
004881 AAGACAAAAA TTTAGAGCTG ATTTTTAAAA GGCTAGTGCT AATATAAAAT CTTCAAATAC TACCCAATGT ATTTTTAGTA 004960
004961 TTAGTAATAA ATATGCCTGT TTATTTGTCC CCAAGAACAT AAAGAGGTCT ATAGAATTTT TAAATGTATA TAGGTTGTTG 005040
005041 TAGGGGTTGT AGGATTTGTT TTTGTGTGTT TTTTTTTTAC TAAAATGATT TTTATTTGTG AATTTTTTAA ATGTTTGTGT 005120
005121 TTTCCTGAAG TAGTAGGAAA TCAATCGTAT TAAGTTATTT TAAACAGTGA AGTGGGAGAT GTATCATTAT ATAGCTTAAT 005200
005201 ATTTAGGGAA AAAAAAGGAA CAAAAATTTA GGTTGTCTTT ACGCTCTAAC TTGACCACAT GGACAAAGGA GGTGATTTGT 005280
005281 ATTTCTTCAA TGATTATATT TTACATACAC AAGTCAAACT TATTTTTTCA ACCTTGAAAT AAAATCATGT AGACAAAAA
000081 GTCGTGATTT TTTCAAAGTT AATCGGCACC ACCTGCATAC CCTCGGCAAC CATTACTCAC CAACACGCCC TCACCGTTCC 000160
000161 CAGTCCCAGT TCTGCGACAT TTAAACTTTT AATCGTGTTC AAATTACCCT ACCATCTTGA ATGTACCAGT AAGCCACACT 000240
000241 TTCAACTTTT TCAGAAGAAT AAAACCCCCA CCTCCTCCGG CCGTAGACAC CGGGGGGAGT TCTCGCTCCC CGCATCCCCG 000320
000321 CGCTTCCCTA CTAACCCCCA CCCGACACCA CCAAAACAAA AGCTATCAGA TTGCAAAAGA CAGCGAATGT GGAGCGACAC 000400
000401 TGCTTATTAA CTACGTACAA AGGAAGTGCG GGGCGGCAGA AGAGAGGGCG ACACGACGCC GACCACAACA AAACCAAGGC 000480
000481 CAGGGCTCCG CGCTCGCAAA ACACAACACC CTCCCCGCGC CGCCGCAGAC CCTGGCGCTG AAACCACAGC GTACGACACC 000560
000561 TCTCGCCAGG GACGGGCGTC TAGGTGTCCC CGTGAGGCGG CGACTACACC CCGCGCTGCG AGCACGTGTG TCGGAATGCA 000640
000641 CGTCGGCTCC GACCACACAC CCACCAACGC ACACTCGGAT GCGGCCCTAG TACCCCTCCA TCCCCCGGCC CGGGAGAAAC 000720
000721 TGGGAGACCA TCCCAGAGCC CTCTGAACTG GGGACCCAGT GGCGCTCCCC GGAAGAGACA AGCTGAGGAA GACTCCCGGC 000800
000801 CTCGCACTTC CCGGGGGGCT GCCCAGAGAG CGCTTCCCCT CACCCCACCG CCCAGCCCCG GGACGCCGGG GGCAGGTAAC 000880
000881 CCAACGAGGA GCGGGCCAAA GTGACGGGCA AGGCCAGGAA CCCGAGGAGC CCGAGGGGCA AAAGCACGTT GGGCGACGGG 000960
000961 AGGCAGCGAC GGGCGCTTGG AGACGTGTCC TGGGGCTTCG GGGCCTCTCC GCAGGGCACC TCCCCCCTCG AGGGCAACTC 001040
001041 GCACCCCGCG GGCCGGGCGG CTGCCCGCTC CCCACTGCCC GGTCAGCCCC AGCAGCGGAG ACGCGGTGCG GCCCAGGGTG 001120
001121 CTGTCGCTGC TGCCTGCGCG CCGCAGGGGA TCAGCGCTGT GTGCGGAGAG GCTCTTGCGC ATGGAAGGGG CGAGGTGCGG 001200
001201 GTCGCGGGGC CCGGGAACCG CGGAGCCCGC CCCGGCACCA ACTTCCCTAC CGAGTCCTCG CGCCTAGCCG GGAAACACGG 001280
001281 CTGGGCTGTC AGGACGGAGA AAGAGAAAAG CCCTTCCCGC GCGGGGATGC GCGGAGCCAG GGCCCGCGGG GCTCCAGACG 001360
001361 AACGAGCCCG GGTCCCGGGT CCGCTGCAGC CTCCCTCCCG GCAGAGCCGC CCTGCGCCCC ACCCCGCCGC TCCCGAGCCC 001440
001441 GGGGGCCCGA CAGCTGCGAC GGTGGCAACT CGGGTTTCGC AAACAGGGCG CAGCCCCTCG GAGGAAAAGT TCCCGCAGTG 001520
001521 GCCGCGGGCG GCGGGCACAT ACTCACTTTC TCCACTCGTC GGCCAGAGCG AGAGCGCGGC GGAGAGAAAC AGGAGCCCCC 001600
001601 ACAGCGAGGT CGGGGACCCT CCTCCGGAGC CAGACTTCAT TCCTTTTATT TGGGATGAAA TTCCCCTTTC TCAAAAAAAA 001680
001681 AAAAAAAAAA AAAAAAGAAA AGAAAAGAAA AAAAGGAAAT GAAAGGAAAC AAGTCTCAAA CTCAGTCTTC GCTCCCCCTC 001760
001761 CAAAAACAAG GGCGAAGGAA ACAATACTCC GAAGGCGGCG CCGCCGGGGC CCTCCGGGTG CGCGGACGGG GCGGGCGGCG 001840
001841 CGGGGCGGCC CCTCAGCGCC GGCAGCAGCG GCCCAGGGCT CGGCGGAGTC GGGAACCCGA GGCGCGCCGG GGCGGGCTGT 001920
001921 CGGCGTCGTC GGCCTCCATT TTCAAACCCG GAGAGGCAGG AAGGGGGGAA AACTGCAAAG AAAGGGGGCA AAGCCCAAAT 002000
002001 CTGCCTAGGC GAGGAAAAAC AAGCCCGGCC CTGGCCGCGC GGGAGGCGGC CGGACGCCCG AGCTTCCGCC GCGGGAGGGC 002080
002081 GCGACGCAGT TCGCAAGATC GCCCCGAAGT CCGGGTCACA GGCGAGGCCG GCGAGGGGCA GAAACGCGGA GTCAAAATGA 002160
002161 ATGAGCGGCT CCCCCTCCTC CTCCTCCTCC TGGCTCCGCT CGCCGCCGCC TCCTCCCTCA GCGCCGCCGC CGCCGCGGCG 002240
002241 AGCTCCTTCC CAAATCCAGG ACACACACAA AGCGGCGGGC CGGCCGCGCC GCGCTCAGCA CTCCCGCCGC GCCGCCCGGC 002320
002321 TCTGCGCCGC CGCCCGCCCC GCGCCGGCCC CCGCCCGGCC CCCGCCCGCC GCCGCCTGCT CTGCGCGGGG GCTCCGGCTC 002400
002401 GCTGAAGGTC ACAGCCGAGG CGAGGCGCGC CGTGGAGCTG CCCCCGCGCG GGAGGGGCGT GGAGGGCGCG GGGGGATCCG 002480
002481 GGGAGCGGGG CCGAGGGTCT GCGAGCGCCG GCCGTGGGCA GGCTCGCTGG CGGCGTCCCT CTCGCTCTCC CGCCTCTCTC 002560
002561 GAGTTCGCCT GGTGCGCTCG CTCCTCCTCG GGTTTTTTCC CTTTTTTTTT CCGCTCAGCG GAGTTAATGC TGGTAAACAA 002640
002641 GAGCCCCAGC CTCGCCGCGC CGCCGCCGCC GCTGCCACAC ACTGGGGGCT CGCGCGCGCG CGCGCGCCCT GGCCCGCGCA 002720
002721 CACGCGCCCG CTCGCACACT CGAGCGGCCA CCGCCGCAGC AGCGGCGCGC CGAGGGCCCG GCCCAGGCCG GCGCGCGCAA 002800
002801 AAAACCGGAG CCCGGTTCCC CAAGACGTGC GGAGCGGAGC CCGGGCGTGG GGCGCGAGAC TGCGAGGCGC CGCCCCCATC 002880
002881 TCCCGCCCCA CCGCCTCCCG CGCGCACACA GAGACACGCG CGCGTGCCCA CCCGCTCCCG CCCCCACCGC ACTGCGCCGG 002960
002961 CCACCCCGCA CGCGCCCTCC CCTCCCGCCC GACTCCAAAA ACAATGCCCC CGCCGCCGGT CCGGTCTCGG CGGGGCAGGG 003040
003041 GTGGGTAGCC AGGGAAAGGC TAGGCAGGGG AGGGGTGGGC AGGAGAGGGG AGGGGTGGGC GGGGAGTGCG GGGCTGGGAG 003120
003121 AGGTTCATTG AAAACAACAA CAGCGCGGCT GGAAAGCGCG TTTCCCCGGT TCTTGAAAAG CTTCATTGTT TCTTGCAGCT 003200
003201 GTTGCAAAAT AAATAAATGC GTGCATGCGG CATTTTTTTC GCTGCGTACT GGAAAGCCCG AACGTGGCGG AAGGGGCGCA 003280
003281 CCTGGGAGAA AGGGGACCCC CCCCCCAGGC TACTACGCCG GGCACTTTCA CGCCAGGCTG GGGTGCCCGC GGCGTTGCGA 003360
003361 CCGTGGAGCC CGGAGTTTGG GGGCTCAGGG GGAGCGGAGG GGGAAGGGCG CGGCCCGTGT GGCTCGGCCC AGAGTGGCTC 003440
003441 GAGGAGAGAG GACCCCGCCG CCCCTCGCGG GCCCCGCGCC CCGGAAGGGG GGCCCAGACA GGCAGCGCCG CGTGCCCCGG 003520
003521 GCGCGTCCCC GCGGCGGCCG CCAGAGGGCG CGGCGGGCGC TGGGGAGGGC AGCCCGCGGA GAGTCGCCTC CCTCCCTCCG 003600
003601 GGGGAGGAAA TGTGGGGGCC CGGGCTGGGG GCCGACTCGG CTGGGTCGCG CTGGCGTGGC CTCCCGGACC AGCCGCCCGC 003680
003681 CCTGAGCGCT CTCCGCGGCC TCGCGCAGCT CAGGGCTCCA CCACGTGGGC GGCTGCGGGG AGCGGCCGGG GCGCGTGCAC 003760
003761 GCTGCGGGTC TCCGAAGCTC GGGGGGCGTG TCCGGGTCCC GGGGCGCCGA GGAGGGGGTG GAGGCTGGGG CCGGGAAGCC 003840
003841 TCCCTTGGCC GCGGTCCCGA CCCGGGAACA CGCCGCATGC ACTCGGGATC AGCGGCTACT GCGGAAACCC ACCCGCACCT 003920
003921 GTCTCGGGTC CTCCCAGGCA CGTTCCTCTT GCTTCGTTTG CTACGTAAGT GACTGGAAAC CAGTACCGAC CCTAAGTCTC 004000
004001 CGCGGTTGTT TTCTTGAATG TGCAGGAGGA GAGGTTTCCC CAAAGCGAGA CTCCCCTAGG ACTGCCATCT AATGAATAAA 004080
004081 TGCTGCGGAG ACCAGGTCCT CAAAGAGGGA AACAGCCCAG CAGTGGCAGG AGAGAGGAGT TTGAAATACG TGGACTTTTA 004160
004161 CAAACAGTGA TTGGACCATG TGTCGCCCTT AATGTGGTCC GGTTTCATGA CTCAGGCTAA TTGATGCCAG TAATAAATTT 004240
004241 TTTTAAGATA GCTAATAAAA GAGTTGTGAA TTGTCGAGAT AAGAAGTGGT TGTCTCAAAC AATCGGGTAG GATGGTAGAA 004320
004321 GGGACCTGAT GTAAAGGAAG GTAGTAGGTT TCTTTTACTC TCTAATTCTA AAATACTTCT GCATTAGAAC GTGTATCCAT 004400
004401 CGTTCGTAAA TAATTTTTTA AGAGCTGAAA ACAAGTCAAA AGCTATCATA ACGGAAACTT AGCCCTTTTT TTCTAAAGTT 004480
004481 GTTTCCTAGA TCATTTTCCT CCTTGGAAAT TTCTAGCTCA ATTCAGAGTC AGTTGTATAG AAAGAAAATA AACCTTGTTT 004560
004561 TACCTAAGAA AGTTATTCAT ATGATGGGAT GGTTTTGTTT AACTGAAATT CAGCTTGATT TTTAAATAAA GAGTTTCAGT 004640
004641 TTTGGATGTT CCTAGATTTA ACAGTGAAAT AAATTTATTA GAATCTTAAA ATGTATTATT TAAAAATTAG GCACTACACA 004720
004721 TGAAGAATTA AAGTCCAGTC TACTAATTTT TAAATATTTC TTAAGTTCTC TGCATGAGGT TGGTCTTGAT TCCTGCAGAT 004800
004801 CCACTCTGGA GTTAAATGCA AAGGAATATT GATATATAAT TTCATTATAC TTGATTTCAG AATTTTTAAT ACATACTTGT 004880
004881 AAGACAAAAA TTTAGAGCTG ATTTTTAAAA GGCTAGTGCT AATATAAAAT CTTCAAATAC TACCCAATGT ATTTTTAGTA 004960
004961 TTAGTAATAA ATATGCCTGT TTATTTGTCC CCAAGAACAT AAAGAGGTCT ATAGAATTTT TAAATGTATA TAGGTTGTTG 005040
005041 TAGGGGTTGT AGGATTTGTT TTTGTGTGTT TTTTTTTTAC TAAAATGATT TTTATTTGTG AATTTTTTAA ATGTTTGTGT 005120
005121 TTTCCTGAAG TAGTAGGAAA TCAATCGTAT TAAGTTATTT TAAACAGTGA AGTGGGAGAT GTATCATTAT ATAGCTTAAT 005200
005201 ATTTAGGGAA AAAAAAGGAA CAAAAATTTA GGTTGTCTTT ACGCTCTAAC TTGACCACAT GGACAAAGGA GGTGATTTGT 005280
005281 ATTTCTTCAA TGATTATATT TTACATACAC AAGTCAAACT TATTTTTTCA ACCTTGAAAT AAAATCATGT AGACAAAAA
