Difference between revisions of "File:Malat1 splicing.jpg"

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(Hypothetical model depicting the role of MALAT1 in AS regulation. (Ea) In normal cells, MALAT1, by associating with SR proteins in nuclear speckles and in the nucleoplasm, regulates their recruitment to the pre-mRNA, thereby regulating AS. Here we hav...)
 
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Hypothetical model depicting the role of MALAT1 in AS regulation.
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tID = 21A|
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(Ea) In normal cells, MALAT1, by associating with SR proteins in nuclear speckles and in the nucleoplasm, regulates their recruitment to the pre-mRNA, thereby regulating AS. Here we have shown SAT1 pre-mRNA as an example that undergoes alternate exon exclusion in normal cells. However, in MALAT1-depleted cells (Eb), cellular levels of SR proteins are increased and are also present predominantly in the dephosphorylated form, resulting in changes in AS of pre-mRNA. In case of SAT1 pre-mRNA, MALAT1 depletion results in the inclusion of an alternative exon containing weak splice sites.
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ltID = 22|
 
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ann = <tab class=wikitable sep=tab head=top>
 
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Section Description
 
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Characteristics ~300 nucleotide, nonpolyadenylated, RNAP III transcript. . SINE (short interspersed nucleotide element - AluJb). 21A lacks the Alu-specific intragenic consensus elements needed to promote its pol III transcription such as the blocks A and B, which points to transcription driven by an extragenic type 3 pol III promoter.. Very low free energy (deltaG) value (deltaG < -100) indicating strong secondary structure.
 
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Expression Nuclear localisation. Detected in tested cultured cells such as HeLa and skin fibroblast cells.. The level of 21A transcription was very low in three immortalized, fully proliferating cell lines (HeLa, 293T, and LAN5) compared to unproliferating/resting PBL (peripheral blood lymphocyte) cells. Similarly, the 21A RNA level in primary skin fibroblasts was higher than in 293T cells, reinforcing an inverse correlation between endogenous 21A expression and cell proliferation.
 
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Function 21AS is one of a number of RNAP III-transcribed short ncRNAs that have sequence complementarity to protein-coding genes, possibly acting as as a natural trans-chromosomal antisense RNA. Aligned to the human genome, it shows several homology hits, among which the most highly significant were associated to multiple intronic regions of centromeric protein F gene (CENP-F). In vitro over-expression of 21AS transcripts inhibits CENP-F protein accumulation and decreased levels of CENP-F mRNA, indicating that 21AS regulates CENP-F expression in trans.. 21A RNAs has a role in the control of the proliferation of human tumor cell lines, possibly via regulation of CENP-F expression. This effect was not observed in a mouse cell line.
 
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Conservation Similarly to all of the 7SL/Alu-derived elements, 21A is primate-specific.
 
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Latest revision as of 04:45, 27 June 2014

Hypothetical model depicting the role of MALAT1 in AS regulation. (Ea) In normal cells, MALAT1, by associating with SR proteins in nuclear speckles and in the nucleoplasm, regulates their recruitment to the pre-mRNA, thereby regulating AS. Here we have shown SAT1 pre-mRNA as an example that undergoes alternate exon exclusion in normal cells. However, in MALAT1-depleted cells (Eb), cellular levels of SR proteins are increased and are also present predominantly in the dephosphorylated form, resulting in changes in AS of pre-mRNA. In case of SAT1 pre-mRNA, MALAT1 depletion results in the inclusion of an alternative exon containing weak splice sites.

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current04:45, 27 June 2014Thumbnail for version as of 04:45, 27 June 2014366 × 628 (40 KB)Lina Ma (talk | contribs)Hypothetical model depicting the role of MALAT1 in AS regulation. (Ea) In normal cells, MALAT1, by associating with SR proteins in nuclear speckles and in the nucleoplasm, regulates their recruitment to the pre-mRNA, thereby regulating AS. Here we hav...
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