Difference between revisions of "ENST00000444200.1"

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Please input one-sentence summary here.
 
Please input one-sentence summary here.
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''APOA1-AS'', an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1, both in vitro and in vivo.
  
 
==Annotated Information==
 
==Annotated Information==
 
===Transcriptomic Nomeclature===
 
===Transcriptomic Nomeclature===
 
Please input transcriptomic nomeclature information here.
 
Please input transcriptomic nomeclature information here.
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''APOA1-AS''
  
 
===Function===
 
===Function===
 
Please input function information here.
 
Please input function information here.
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''APOA1-AS'' acts analogous to a molecular switch to dictate the expression levels of APOA1. APOA1-AS may locally regulate APO gene cluster. APOA1-AS can modulate distinct histone methylation patterns that mark active and/or inactive gene expression, through the recruitment of histone-modifying enzymes. Targeting APOA1-AS using short antisense oligonucleotides also enhanced APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.<ref name="ref1" />
  
 
===Regulation===
 
===Regulation===
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===Expression===
 
===Expression===
 
Please input expression information here.
 
Please input expression information here.
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APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, ''APOA1-AS'' is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/''APOA1-AS'' varies among the tissues examined (list the examined tissues).<ref name="ref1" />
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{| class='wikitable' style="text-align:center"
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|-
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! | SiRNA name
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! | Sequence
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|-
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| rowspan="1"|APOA1-AS1
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| | 5'-CCAAATGGCAAACCTTCTT-3'<ref name="ref1" />
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|-
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| rowspan="1"|APOA1-AS2
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| | 5'-GGCTACTTATTGCTGGAAA-3'<ref name="ref1" />
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|-
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| rowspan="1"|APOA1-AS3
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| | 5'-CCTTGGATTGAAGTAATC-3'<ref name="ref1" />
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|}
  
 
===Allelic Information and Variation===
 
===Allelic Information and Variation===
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==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
 
Please input related labs here.
 
Please input related labs here.
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Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine <ref name="ref1" />.
 
==References==
 
==References==
 
Please input cited references here.
 
Please input cited references here.
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<references>
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<ref name="ref1"> Halley P, Kadakkuzha BM, Faghihi MA, Magistri M, Zeier Z, Khorkova O, et al. Regulation of the apolipoprotein gene cluster by a long noncoding RNA[J]. Cell reports. 2014,6(1):222-30.
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</ref>(1)
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</references>
  
 
{{basic|
 
{{basic|

Revision as of 06:49, 2 April 2015

Please input one-sentence summary here. APOA1-AS, an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1, both in vitro and in vivo.

Annotated Information

Transcriptomic Nomeclature

Please input transcriptomic nomeclature information here. APOA1-AS

Function

Please input function information here. APOA1-AS acts analogous to a molecular switch to dictate the expression levels of APOA1. APOA1-AS may locally regulate APO gene cluster. APOA1-AS can modulate distinct histone methylation patterns that mark active and/or inactive gene expression, through the recruitment of histone-modifying enzymes. Targeting APOA1-AS using short antisense oligonucleotides also enhanced APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.[1]

Regulation

Please input regulation information here.

Expression

Please input expression information here. APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, APOA1-AS is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/APOA1-AS varies among the tissues examined (list the examined tissues).[1]

SiRNA name Sequence
APOA1-AS1 5'-CCAAATGGCAAACCTTCTT-3'[1]
APOA1-AS2 5'-GGCTACTTATTGCTGGAAA-3'[1]
APOA1-AS3 5'-CCTTGGATTGAAGTAATC-3'[1]

Allelic Information and Variation

Please input allelic information and variation information here.

Evolution

Please input evolution information here.

You can also add sub-section(s) at will.

Labs working on this lncRNA

Please input related labs here. Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine [1].

References

Please input cited references here.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Halley P, Kadakkuzha BM, Faghihi MA, Magistri M, Zeier Z, Khorkova O, et al. Regulation of the apolipoprotein gene cluster by a long noncoding RNA[J]. Cell reports. 2014,6(1):222-30.

Basic Information

Transcript ID

ENST00000444200.1

Source

Gencode19

Same with

lnc-APOC3-1:1,NONHSAT024360

Classification

antisense

Length

563 nt

Genomic location

chr11+:116706833..116726445

Exon number

2

Exons

116706833..116706955,116726006..116726445

Genome context

Sequence
000001 CAGCTTCTCT TGCAGCTCGT GCAGCTTCTG GCGCGCGCCC TCTTGGAGCT CTGCGCGCAG CGGCTCCACC TTCTGGCGGT 000080
000081 AGAGCTCCAT CTCCTCCTGC CACTTCTTCT GGAAGTCGTC CAGATCCAAA TGGCAAACCT TCTTCATCCA CCAGGACCCA 000160
000161 ACCCACAGGC TACTTATTGC TGGAAACCTA CGTTGTTCCT TGGATTGAAG TAATCTCTCC CTCCTCTGGT GCGCCCACAG 000240
000241 CACTTGCACC AACAGTGGGT ACTCAACAGA CTAGCGTGCC TGCCGAAGAA GGGGTCCTCT GACAATCAGG GGACAATGGG 000320
000321 GAATTATGCT CTCCAGACTT TCTACACACA CAAGTCACAC AGGAAGGAAG GTAAAGAGAA ACTAGAGAAA ATAATTTTTG 000400
000401 AAGAAAAACA TTTCAGGAAG TATTGAAAGT ACACGGTAAC TCAGCCTGGG GCAGGGGTGG AGGGCAGCAG CACTGTTTGC 000480
000481 TGCAGCTATG CTCCTTCCTC AGTGCCCTGC ACACCCGGGA CTTGCTCGGT GAGCATCTCT CGTGTCAGTG ACAGCTAGTG 000560
000561 TGA
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