Difference between revisions of "ENST00000444200.1"
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''APOA1-AS'', an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1, both in vitro and in vivo. | ''APOA1-AS'', an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1, both in vitro and in vivo. | ||
==Annotated Information== | ==Annotated Information== | ||
===Transcriptomic Nomeclature=== | ===Transcriptomic Nomeclature=== | ||
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''APOA1-AS'' | ''APOA1-AS'' | ||
===Function=== | ===Function=== | ||
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''APOA1-AS'' acts analogous to a molecular switch to dictate the expression levels of APOA1. APOA1-AS may locally regulate APO gene cluster. APOA1-AS can modulate distinct histone methylation patterns that mark active and/or inactive gene expression, through the recruitment of histone-modifying enzymes. Targeting APOA1-AS using short antisense oligonucleotides also enhanced APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.<ref name="ref1" /> | ''APOA1-AS'' acts analogous to a molecular switch to dictate the expression levels of APOA1. APOA1-AS may locally regulate APO gene cluster. APOA1-AS can modulate distinct histone methylation patterns that mark active and/or inactive gene expression, through the recruitment of histone-modifying enzymes. Targeting APOA1-AS using short antisense oligonucleotides also enhanced APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.<ref name="ref1" /> | ||
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===Expression=== | ===Expression=== | ||
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APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, ''APOA1-AS'' is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/''APOA1-AS'' varies among the tissues examined (list the examined tissues).<ref name="ref1" /> | APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, ''APOA1-AS'' is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/''APOA1-AS'' varies among the tissues examined (list the examined tissues).<ref name="ref1" /> | ||
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==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
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Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine <ref name="ref1" />. | Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine <ref name="ref1" />. | ||
==References== | ==References== | ||
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<references> | <references> | ||
<ref name="ref1"> Halley P, Kadakkuzha BM, Faghihi MA, Magistri M, Zeier Z, Khorkova O, et al. Regulation of the apolipoprotein gene cluster by a long noncoding RNA[J]. Cell reports. 2014,6(1):222-30. | <ref name="ref1"> Halley P, Kadakkuzha BM, Faghihi MA, Magistri M, Zeier Z, Khorkova O, et al. Regulation of the apolipoprotein gene cluster by a long noncoding RNA[J]. Cell reports. 2014,6(1):222-30. | ||
Revision as of 06:50, 2 April 2015
APOA1-AS, an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1, both in vitro and in vivo.
Contents
Annotated Information
Transcriptomic Nomeclature
APOA1-AS
Function
APOA1-AS acts analogous to a molecular switch to dictate the expression levels of APOA1. APOA1-AS may locally regulate APO gene cluster. APOA1-AS can modulate distinct histone methylation patterns that mark active and/or inactive gene expression, through the recruitment of histone-modifying enzymes. Targeting APOA1-AS using short antisense oligonucleotides also enhanced APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.[1]
Regulation
Please input regulation information here.
Expression
APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, APOA1-AS is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/APOA1-AS varies among the tissues examined (list the examined tissues).[1]
| SiRNA name | Sequence |
|---|---|
| APOA1-AS1 | 5'-CCAAATGGCAAACCTTCTT-3'[1] |
| APOA1-AS2 | 5'-GGCTACTTATTGCTGGAAA-3'[1] |
| APOA1-AS3 | 5'-CCTTGGATTGAAGTAATC-3'[1] |
Allelic Information and Variation
Please input allelic information and variation information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this lncRNA
Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine [1].
References
Basic Information
| Transcript ID |
ENST00000444200.1 |
| Source |
Gencode19 |
| Same with |
lnc-APOC3-1:1,NONHSAT024360 |
| Classification |
antisense |
| Length |
563 nt |
| Genomic location |
chr11+:116706833..116726445 |
| Exon number |
2 |
| Exons |
116706833..116706955,116726006..116726445 |
| Genome context |
|
| Sequence |
000001 CAGCTTCTCT TGCAGCTCGT GCAGCTTCTG GCGCGCGCCC TCTTGGAGCT CTGCGCGCAG CGGCTCCACC TTCTGGCGGT 000080
000081 AGAGCTCCAT CTCCTCCTGC CACTTCTTCT GGAAGTCGTC CAGATCCAAA TGGCAAACCT TCTTCATCCA CCAGGACCCA 000160 000161 ACCCACAGGC TACTTATTGC TGGAAACCTA CGTTGTTCCT TGGATTGAAG TAATCTCTCC CTCCTCTGGT GCGCCCACAG 000240 000241 CACTTGCACC AACAGTGGGT ACTCAACAGA CTAGCGTGCC TGCCGAAGAA GGGGTCCTCT GACAATCAGG GGACAATGGG 000320 000321 GAATTATGCT CTCCAGACTT TCTACACACA CAAGTCACAC AGGAAGGAAG GTAAAGAGAA ACTAGAGAAA ATAATTTTTG 000400 000401 AAGAAAAACA TTTCAGGAAG TATTGAAAGT ACACGGTAAC TCAGCCTGGG GCAGGGGTGG AGGGCAGCAG CACTGTTTGC 000480 000481 TGCAGCTATG CTCCTTCCTC AGTGCCCTGC ACACCCGGGA CTTGCTCGGT GAGCATCTCT CGTGTCAGTG ACAGCTAGTG 000560 000561 TGA |
