Difference between revisions of "ENST00000412690.1"
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LINC01191: long intergenic non-protein coding RNA 1191(HGNC nomenclature) | LINC01191: long intergenic non-protein coding RNA 1191(HGNC nomenclature) | ||
| − | ''lnc-ACTR3'', ''VIN'', "virus inducible | + | ''lnc-ACTR3'', ''VIN'', "virus inducible lincRNA" <ref name="ref1" /> |
===Characteristics=== | ===Characteristics=== | ||
| Line 23: | Line 23: | ||
===Disease=== | ===Disease=== | ||
| − | + | VIN is functionally relevant during pathogenesis of IAV infection. <ref name="ref1" /> | |
===Expression=== | ===Expression=== | ||
| − | VIN expression seems to be a specific response to certain viral infections including IAV and VSV. <ref name="ref1" /> | + | VIN expression seems to be a specific response to certain viral infections including IAV and VSV, suggesting that this lincRNA may have broader functionality during virus infection. IBV, viral RNA mimics, or IFNβ are not able to induce VIN, this induction is likely to be a specific response and not due the presence of viral RNA itself.<ref name="ref1" /> |
{|class='wikitable' style="text-align:center" | {|class='wikitable' style="text-align:center" | ||
|- | |- | ||
| − | ! | | + | ! |Experiment |
| − | ! | | + | ! |Primer |
| + | ! |Forward | ||
| + | ! |Reverse | ||
|- | |- | ||
| − | | rowspan="1"|IAV Nucleoprotein | + | | rowspan="1"|qRT-PCR |
| + | | | VIN | ||
| + | | | CTAGGAGACACCCGGACAGT | ||
| + | | | GCCCTGTGAGATGGGTTTAG<ref name="ref1" /> | ||
| + | |- | ||
| + | | rowspan="4"|Knockdown | ||
| + | | | IAV Nucleoprotein | ||
| | AAGGAUCUUAUUUCUUCGGAG<ref name="ref1" /> | | | AAGGAUCUUAUUUCUUCGGAG<ref name="ref1" /> | ||
| + | | |_ | ||
|- | |- | ||
| − | | | + | | | VIN siRNA 1 |
| | CTGTGACATGTAGATTGCTAA<ref name="ref1" /> | | | CTGTGACATGTAGATTGCTAA<ref name="ref1" /> | ||
| + | | |_ | ||
|- | |- | ||
| − | | | + | | | VIN siRNA 2 |
| | CCGGAGCCGTTTACAGTTTGA<ref name="ref1" /> | | | CCGGAGCCGTTTACAGTTTGA<ref name="ref1" /> | ||
| + | | |_ | ||
|- | |- | ||
| − | | | + | | | VIN siRNA 3 |
| | CGCGCCCTGTCCCGCCATATA<ref name="ref1" /> | | | CGCGCCCTGTCCCGCCATATA<ref name="ref1" /> | ||
| + | | |_ | ||
|} | |} | ||
Revision as of 08:50, 1 July 2016
LINC01191, a 869bp long non-coding RNA, which is induced by several IAV(influenza A virus) strains (H1N1, H3N2, H7N7) as well as vesicular stomatitis virus, functions in IAV propagation.
Contents
Annotated Information
Name
LINC01191: long intergenic non-protein coding RNA 1191(HGNC nomenclature)
lnc-ACTR3, VIN, "virus inducible lincRNA" [1]
Characteristics
LINC01191,virus inducible lincRNA (VIN), is induced by several IAV(influenza A virus) strains (H1N1, H3N2, H7N7) as well as vesicular stomatitis virus. [1]
Cellular Localization
VIN is localized to the host cell nucleus. [1]
Function
VIN expression was specifically induced upon infection with a number of IAV and VSV(vesicular stomatitis virus), suggesting that this lincRNA may have broader functionality during virus infection.
VIN adopts stable secondary structures, and thus, has a functional role in cells, perhaps in complex with other cellular components as it was largely insensitive to endonuclease.
Nuclear expression of VIN suggests an involvement in gene-regulatory processes and VIN is functionally relevant during pathogenesis of IAV infection which strengthens the view that lncRNAs are major players in diverse biological processes. [1]
Disease
VIN is functionally relevant during pathogenesis of IAV infection. [1]
Expression
VIN expression seems to be a specific response to certain viral infections including IAV and VSV, suggesting that this lincRNA may have broader functionality during virus infection. IBV, viral RNA mimics, or IFNβ are not able to induce VIN, this induction is likely to be a specific response and not due the presence of viral RNA itself.[1]
| Experiment | Primer | Forward | Reverse |
|---|---|---|---|
| qRT-PCR | VIN | CTAGGAGACACCCGGACAGT | GCCCTGTGAGATGGGTTTAG[1] |
| Knockdown | IAV Nucleoprotein | AAGGAUCUUAUUUCUUCGGAG[1] | _ |
| VIN siRNA 1 | CTGTGACATGTAGATTGCTAA[1] | _ | |
| VIN siRNA 2 | CCGGAGCCGTTTACAGTTTGA[1] | _ | |
| VIN siRNA 3 | CGCGCCCTGTCCCGCCATATA[1] | _ |
Labs working on this lncRNA
- Department of Molecular Biology; Max Planck Institute for Infection Biology; Berlin, Germany[1]
References
Basic Information
| Transcript ID |
ENST00000412690.1 |
| Source |
Gencode19 |
| Same with |
lnc-ACTR3-1:1,LINC01191,lnc-ACTR3, VIN |
| Classification |
intergenic |
| Length |
844 nt |
| Genomic location |
chr2+:114737146..114764879 |
| Exon number |
2 |
| Exons |
114737146..114737705,114764596..114764879 |
| Genome context |
|
| Sequence |
000001 CCGGATTCCG AAGCCCGGAG CCGTTTACAG TTTGAGGCGC CGTGTGCCGC CCCCTGGTGG CCGGAGGCCG TTACTGTCGC 000080
000081 GGCTGAGGGG GCGTCCCGGT GTATCCGCAG CGCGCCCTGG CCGCCCTCTG GTGGCCGGAG GCCGTTACTG TCGCGGCTGA 000160 000161 GGGGGCGTCC CGGTGTATCC GCAGCGCGCC CTGGCCGCCC CCTGGTGGCC GGAGGCCGTT ACTGTCGCGG CTGAGGGGGC 000240 000241 GTCCCGGTGT ATCCGCAGCG CGCCCTGGCC GACCCCTGGT GGCCGGAGGC CGTTACTGTC GCGGCTGAAG GGGCGTGTCC 000320 000321 CGGTGTATCC GCAGCGCGCC CTGGCTGCCC CCTGGTGGCC GGAGGCCGTT ACTGTCGCGG CTGAGGGGGC GTCCCGGTGT 000400 000401 ATCCGCAGCG CGCCCTGTCC CGCCATATAC ATTCCGTGCT TTTCAAACAC CCGCCGTGGG CTAAGCATGG GACCCACGTG 000480 000481 GGGCGGGGAC ACACGGCGAT AAAGAGCCAG GCCCTCGCTG CCAAGGAGCT TTCCCGTAAC ACCGATGCAT CAAACAAAAG 000560 000561 GCAGCAGTGA GGATGCCAGA GTTCATGGGT TTCTAGAAGA CAAGTTTTCA ACTGCAGATA CAAACATCAG GACGTCAATG 000640 000641 TATCTGCTAG GAGACACCCG GACAGTGTTG AGAACACAGA ACCTGGAGGC TAAACCCCCA GCTTTCTATG CCAGCTGAGA 000720 000721 GTCTGTGGGC ACATTACTCA GCGTCTCTGA ACCTCATGCT CCTCCCCTGT GACATGTAGA TTGCTAAACC CATCTCACAG 000800 000801 GGCTATCTTC TGCATTTAAA GAATAAAATA ACACATGAGG GGTC |
