Difference between revisions of "GSC-DT"

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(Created page with "''GSC-DT'', is a conserved long noncoding RNA and a key regulator of human and mouse definitive endoderm (DE) differentiation. ==Annotated Information== ===Name=== ''GSC-DT'':...")
 
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===Function===
 
===Function===
 
[[File:GSC-DT.jpg|right|thumb|400px|'''Loss of ''GSC-DT'' expression inhibits definitive endoderm differentiation.''' <ref name="ref1" />]]
 
[[File:GSC-DT.jpg|right|thumb|400px|'''Loss of ''GSC-DT'' expression inhibits definitive endoderm differentiation.''' <ref name="ref1" />]]
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''GSC-DT'' regulates Goosecoid (GSC) expression to control definitive endoderm differentiation of both human and mouse embryonic stem cells <ref name="ref1" />.  
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''GSC-DT'' regulates Goosecoid (GSC) expression to control definitive endoderm differentiation of both human and mouse embryonic stem cells <ref name="ref1" />.  
  
 
Goosecoid (GSC) expression is regulated by ''GSC-DT'' in trans, and the defect in definitive endoderm (DE) differentiation present in  ''GSC-DT''-deficient hESCs can be rescued by induction of GSC. <ref name="ref1" />.  
 
Goosecoid (GSC) expression is regulated by ''GSC-DT'' in trans, and the defect in definitive endoderm (DE) differentiation present in  ''GSC-DT''-deficient hESCs can be rescued by induction of GSC. <ref name="ref1" />.  
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===Regulation===
 
===Regulation===
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[[File:GSC-DT regulation.jpg|right|thumb|400px|'''''GSC-DT'' regulation through SMAD3.''' <ref name="ref1" />]]
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[[File:GSC-DT regulation.jpg|right|thumb|200px|'''''GSC-DT'' regulation through SMAD3.''' <ref name="ref1" />]]
 
''GSC-DT''  regulated by an enhancer bound by SMAD3 following activin signaling during endoderm differentiation <ref name="ref1" />.
 
''GSC-DT''  regulated by an enhancer bound by SMAD3 following activin signaling during endoderm differentiation <ref name="ref1" />.
  

Revision as of 05:08, 27 December 2018

GSC-DT, is a conserved long noncoding RNA and a key regulator of human and mouse definitive endoderm (DE) differentiation.

Annotated Information

Name

GSC-DT: GSC divergent transcript (HGNC nomenclature), DIGIT [1].

Characteristics

GSC-DT is two exon transcript of 2226 bp long non-coding RNA (GenBank), located on chromosome chr14q32.13 (HGNC).

Function

Loss of GSC-DT expression inhibits definitive endoderm differentiation. [1]

GSC-DT regulates Goosecoid (GSC) expression to control definitive endoderm differentiation of both human and mouse embryonic stem cells [1].

Goosecoid (GSC) expression is regulated by GSC-DT in trans, and the defect in definitive endoderm (DE) differentiation present in GSC-DT-deficient hESCs can be rescued by induction of GSC. [1].

Expression

GSC-DT is expressed during embryonic development [1].

Regulation

GSC-DT regulation through SMAD3. [1]

GSC-DT regulated by an enhancer bound by SMAD3 following activin signaling during endoderm differentiation [1].

Diseases

NA

Labs working on this lncRNA

  • Gastrointestinal Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 USA.[1]
  • Harvard Stem Cell Institute, Cambridge, MA 02138 USA.[1]
  • School of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.[1]
  • Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.[1]
  • Department of Veterinary and Animal Sciences, University of Massachusetts at Amherst, Amherst, MA 01003 USA.[1]

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA et al. DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC Expression to Control Definitive Endoderm Differentiation of Embryonic Stem Cells[J]. Cell Rep. 2016, 17(2):353-365.

Sequence

>gi|108868751|ref|NR_144432.1| Homo sapiens GSC divergent transcript (GSC-DT), long non-coding RNA

000001 AAATTCATTG TGGACGATTT TACTTTGGGG CTACTTTTTT CCCCCTTTCG GAATTGGTTT TGTTTTAGAA AATGTGAGGG 000080
000081 GCTGCATCCC CTCTCAAGCA GGATTGAAAA TTCCACCACT CCTCCGCGCC CCCACGTACT CACCGCCTGA TTTACCTCTT 000160
000161 TTTTAAATCT TGAGTTTCTC TCCCGAATGG AACTTGTTAA CCCCCCCAAA AAACTTTTGG TTTTGTAAAA TCCAAATTTT 000240
000241 GTAAAGGCAA AAACGACTAA AGGAGGGGTG CATCGTGCTT CTTAAAAAGA TTTTTTTTTC CAGCCACTCT GGGTCTGAAA 000320
000321 ACTACCATGG CAGTCCCTAG GTCCTACACA GCGAAGACCA AATTCAGCCG CTCTTTAAAA TTCGCCACCA AGAGTTGCAG 000400
000401 TCTGAAGCGG TGCTTCTAGC TGCTCCCAGC GTGGGTGTCG GTAGTTTTTG TGCTTTTAAA AAATTAGTTT TGGAAATGTG 000480
000481 GTGCCTTCTC TCCCAAACTA TTTTTTTGAT TTTCAATCCA GATGGGGTCT TTGGGACTTC CCTATCTTCT TCCCCAATAA 000560
000561 TTGGGTCGCA TTTTTCCTTA CGACCTGCTG GCTGCCGGTA GGCCTGCCCT TCCGCCTGGC AGGCCCAGAG TGGATTCCCT 000640
000641 GGGCCGCAGT GGCCTGGACG ATGGCCGGGT CTGATAGCGG CAGGCGGCCG CGATGATTTG CGGGATTCTC GGCATGGCAC 000720
000721 GGCCAGCCTG CCCGAGCCAC GGGAGGCGCA GCCAGCCCAC CACTCACGGC AAGCAGGCGG CCGAGCGCCC TTCGAGAAGA 000800
000801 GACTTAAGTC ACCATGCTCC AGGGCAGATA CTCCCCCACC AATCTTTCCC AGGTTTAAAG CTACAGGGCA GTTGATGGAC 000880
000881 GACAACTAGA GGCTGCAACT TATCAGTGAC TGCCTGCGTT TGTGTTGGAG GCTGGGTGTG CGCTGCGGTG CGCGTGTGAG 000960
000961 TGGATCTTTT AAGACCTTTG TCTGAGCCGG GCAGAGGCAC AAGTGCCACC TGACGCTTGG GGCAGATTCC TCGTCCTGTT 001040
001041 CTTGCAAAGA TGCCAAATCA GGAGAGTGTC AAGATTTCAA CCGCAGACCT AAAAAAAGTG TCTCCATGTT TTTAAAAGTA 001120
001121 TCAGACAACC ACCTCCACAC ACCCCTCCGT CTCCCCTTCC ACCTGGAACA TTGTTCTTTT TAATAACCAG GTTTAGCAAA 001200
001201 CTGCTATACT GCGCCTGTGC TTTCCTGCAG CCCTCCGCTG TACCAGCTGA GCCCTGTACA GAATCTGAGC GTCAGAGTTG 001280
001281 CAGACCCAAC CTTGCCACCT TGCAAATCAT CCCTTCTCTG GAGGCCTCAG GATTTTAGTT GATCAAACCA ACACCAACAT 001360
001361 TCATTGGGTC CCTTTGCAAC CCTAACATTT AATGAGACTT GGTAATTGTC ATCCGTTCGC CAAGGAGGTT GGGGATGAAC 001440
001441 TTGGGGGCAT TTTCTTGTTT ATTTTTCATT GAGACAAAGG AGTGTGCTGT CCCCACCTCC GCCCCCAGAC AAGGACAGGG 001520
001521 TTCCTACATC AGCTGGGTCT AAAATAAGGA AAAGCGAGTG CTGCCGCAGA GGGGACTTTC CGGGAAGGGG GTCACAGGAA 001600
001601 CCCCAAGGCC ACACAGCTCC AGACACTCTG CCGGAGCTCT AGGGGAGCTC ACCCCTGCGC GGCGGGACCT CCCCCATCGG 001680
001681 GTTGCCCCTT CAATCTGCAG CCGCGCGGAG GCAGGCCCCG GGAAAGGCAG TCGGCGCTCG GTGCTCGCAC TGCGCCTCGG 001760
001761 CGGGCGGAAA CTCACAAGTG TCCTCAACGT GCTAAACAAA CCATCAGGGC CCCGCATCGC CTGGCGCAAT TGGAATAACA 001840
001841 AATGCGACGC ACGCAAATTG TCCACCTCGT GTTG