Difference between revisions of "NONHSAT028496"
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| − | + | ''CISTR'', a chondrogenic regulator, interacts in cis with PTHLH expression and simultaneously in trans with sex-determing region Y-box9(SOX9). | |
==Annotated Information== | ==Annotated Information== | ||
===Transcriptomic Nomeclature=== | ===Transcriptomic Nomeclature=== | ||
| − | + | ''CISTR'':chondrogenesis-associated transcript (HGNC nomenclature) | |
===Function=== | ===Function=== | ||
| − | + | [[File:DA125942 transcription and dependent regulation of SOX9, DA125942, and PTHLH..jpg|right|thumb|400px|'''DA125942 transcription and dependent regulation of SOX9, DA125942, and PTHLH.''' <ref name="ref1" />.]] | |
| + | |||
| + | ''CISTR'' silencing led to PTHLH or SOX9 repressions in humans and rodents, whereas their altered expression coincided with lncRNA dysregulation. | ||
===Regulation=== | ===Regulation=== | ||
| − | + | Knocking down PTHLH and SOX9 significantly increased ''CISTR'' expression by 4- and 6.8-fold,respectively. | |
===Expression=== | ===Expression=== | ||
| − | + | In the BDE patients, the ''CISTR'' occupancy at the PTHLH locus was reduced. | |
| + | |||
| + | {| class='wikitable' style="text-align:center" | ||
| + | |- | ||
| + | ! | siRNA | ||
| + | ! | sense | ||
| + | ! | antisense | ||
| + | |- | ||
| + | | rowspan="1"|siRNA 1 | ||
| + | | | GCACAUGACCACAUGGAAATT | ||
| + | | | UUUCCAUGUGGUCAUGUGCGA <ref name="ref1" /> | ||
| + | |- | ||
| + | | rowspan="1"|siRNA 2 | ||
| + | | | GUUUCUCUCAGCAAAUUCATT | ||
| + | | | UGAAUUUGCUGAGAGAAACTG <ref name="ref1" /> | ||
| + | |- | ||
| + | | rowspan="1"|siRNA 3 | ||
| + | | | CAAGGACACUGAAAAAGCUTT | ||
| + | | | AGCUUUUUCAGUGUCCUUGAG <ref name="ref1" /> | ||
| + | |} | ||
===Allelic Information and Variation=== | ===Allelic Information and Variation=== | ||
| Line 23: | Line 44: | ||
==Labs working on this lncRNA== | ==Labs working on this lncRNA== | ||
| − | + | Experimental and Clinical Research Center (ECRC), a joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany. | |
==References== | ==References== | ||
| − | + | <references> | |
| + | <ref name="ref1">Maass PG, Rump A, Schulz H, Stricker S, Schulze L, Platzer K, et al. A misplaced lncRNA causes brachydactyly in humans[J]. The Journal of clinical investigation. 2012,122(11):3990-4002.<ref name="ref1" /> | ||
| + | </references> | ||
{{basic| | {{basic| | ||
Revision as of 16:51, 2 April 2015
CISTR, a chondrogenic regulator, interacts in cis with PTHLH expression and simultaneously in trans with sex-determing region Y-box9(SOX9).
Contents
Annotated Information
Transcriptomic Nomeclature
CISTR:chondrogenesis-associated transcript (HGNC nomenclature)
Function
DA125942 transcription and dependent regulation of SOX9, DA125942, and PTHLH. [1].
CISTR silencing led to PTHLH or SOX9 repressions in humans and rodents, whereas their altered expression coincided with lncRNA dysregulation.
Regulation
Knocking down PTHLH and SOX9 significantly increased CISTR expression by 4- and 6.8-fold,respectively.
Expression
In the BDE patients, the CISTR occupancy at the PTHLH locus was reduced.
| siRNA | sense | antisense |
|---|---|---|
| siRNA 1 | GCACAUGACCACAUGGAAATT | UUUCCAUGUGGUCAUGUGCGA [1] |
| siRNA 2 | GUUUCUCUCAGCAAAUUCATT | UGAAUUUGCUGAGAGAAACTG [1] |
| siRNA 3 | CAAGGACACUGAAAAAGCUTT | AGCUUUUUCAGUGUCCUUGAG [1] |
Allelic Information and Variation
Please input allelic information and variation information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this lncRNA
Experimental and Clinical Research Center (ECRC), a joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
References
Basic Information
| Transcript ID |
NONHSAT028496 |
| Source |
NONCODE4.0 |
| Same with |
, |
| Classification |
intergenic |
| Length |
772 nt |
| Genomic location |
chr12-:54144081..54145660 |
| Exon number |
2 |
| Exons |
54144081..54144749,54145558..54145660 |
| Genome context |
|
| Sequence |
000001 gggaggaaaa gaggcgggag agagaCCCTA AAAAGCAAGC TAGACTCTCC AGCCGGCCGC TCTGGTGTGG GGAGGGAGCG 000080
000081 TCATCTCAAG GACACTGAAA AAGCTGTTTG CCGTTTTGCT GTTTGCCTCC TTGTCACGAC CTCAGCGACG GCAGATGGAG 000160 000161 CCAGTGGAGG GAGAGAGACA GACGGCAATG CTTGGGTAGC CAGGGCTCCC TGCCGGCCCC ACTCCTCCTG CAGACACGAG 000240 000241 CACGCACACA CACACGCGCG CAAAACACAC ACGCTGCCCT TCGCCACACG TAGCCAAGTA AAATACACAA AAAGCAACTG 000320 000321 AGATACCATT CGCACATGAC CACATGGAAA CACACAAATG AACAAACGCA TTCAGGCATA GCCACATGCC AGTCACACAG 000400 000401 ATGGATAGAC AAGAGCCTGG GGTACAAAGC CACATGGCAC AAAGCCACCT GGATGTATAT GCCCCTacac acgcacacag 000480 000481 aaaaaaacat gcaaacttaa gtacacacct atccagacaa gtgtgagtgc acacacacac acactcacac acTCTACCTG 000560 000561 ACACGTTAGT GCATCCCCAT TGGGCCCCAG TTTCTCTCAG CAAATTCAGA TCACCCAGCC TGACTTCAAA ACCTGCCCCC 000640 000641 TTCCATTCCC CTTCATGCTG GTTCCTGAAT CCAGCTCGTT GCTCCAGGCT AGCAAAGCTG TCCACAGCCT CATTTGTGGT 000720 000721 TTGCTCACCT GCCTCAGCAC CATCAGCCAG AGGTACTCCT GCTCTTCTCC TA |
