Difference between revisions of "ENST00000444200.1"
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| − | ''APOA1-AS'', an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1 | + | ''APOA1-AS'', an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1 by modulating distinct histone methylation patterns. |
==Annotated Information== | ==Annotated Information== | ||
| − | === | + | ===Name=== |
| − | ''APOA1-AS'' | + | ''APOA1-AS'': Apolipoprotein A-1 antisense RNA(HGNC nomenclature) |
| + | |||
| + | ===Characteristics=== | ||
| + | ''APOA1-AS'' is transcribed from the positive strand of chromosome 11 and has two exons, positioned ~20kb apart. It shares a 123 nucleotide-long region overlapping with the fourth exon of APOA1 mRNA.(Figure 1) | ||
===Function=== | ===Function=== | ||
| − | ''APOA1-AS'' | + | ''APOA1-AS'' functions in cis to modulate histone methylation patterns of APOA1, through the recruitment of LSD1 and PRC2. Targeting APOA1-AS using short antisense oligonucleotides also enhances the expression level of APOC3 and APOA4, which are located in close proximity on the chromatin. APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.<ref name="ref1" /> |
===Regulation=== | ===Regulation=== | ||
Revision as of 11:47, 5 April 2015
APOA1-AS, an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1 by modulating distinct histone methylation patterns.
Contents
Annotated Information
Name
APOA1-AS: Apolipoprotein A-1 antisense RNA(HGNC nomenclature)
Characteristics
APOA1-AS is transcribed from the positive strand of chromosome 11 and has two exons, positioned ~20kb apart. It shares a 123 nucleotide-long region overlapping with the fourth exon of APOA1 mRNA.(Figure 1)
Function
APOA1-AS functions in cis to modulate histone methylation patterns of APOA1, through the recruitment of LSD1 and PRC2. Targeting APOA1-AS using short antisense oligonucleotides also enhances the expression level of APOC3 and APOA4, which are located in close proximity on the chromatin. APOA1 expression in both human and monkey liver cells, and induced an increase in hepatic RNA and protein expression in African green monkeys.[1]
Regulation
Please input regulation information here.
Expression
APOA1 mRNA’s expression level is highest in liver, an order of magnitude less in small intestine and two orders of magnitude less in colon . However, APOA1-AS is highly expressed in ovary, cervix, testis and thyroid. It is found that the ratio of APOA1/APOA1-AS varies among the tissues examined (list the examined tissues).[1]
| SiRNA name | Sequence |
|---|---|
| APOA1-AS1 | 5'-CCAAATGGCAAACCTTCTT-3'[1] |
| APOA1-AS2 | 5'-GGCTACTTATTGCTGGAAA-3'[1] |
| APOA1-AS3 | 5'-CCTTGGATTGAAGTAATC-3'[1] |
Allelic Information and Variation
Please input allelic information and variation information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this lncRNA
Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine [1].
References
Basic Information
| Transcript ID |
ENST00000444200.1 |
| Source |
Gencode19 |
| Same with |
lnc-APOC3-1:1,NONHSAT024360 |
| Classification |
antisense |
| Length |
563 nt |
| Genomic location |
chr11+:116706833..116726445 |
| Exon number |
2 |
| Exons |
116706833..116706955,116726006..116726445 |
| Genome context |
|
| Sequence |
000001 CAGCTTCTCT TGCAGCTCGT GCAGCTTCTG GCGCGCGCCC TCTTGGAGCT CTGCGCGCAG CGGCTCCACC TTCTGGCGGT 000080
000081 AGAGCTCCAT CTCCTCCTGC CACTTCTTCT GGAAGTCGTC CAGATCCAAA TGGCAAACCT TCTTCATCCA CCAGGACCCA 000160 000161 ACCCACAGGC TACTTATTGC TGGAAACCTA CGTTGTTCCT TGGATTGAAG TAATCTCTCC CTCCTCTGGT GCGCCCACAG 000240 000241 CACTTGCACC AACAGTGGGT ACTCAACAGA CTAGCGTGCC TGCCGAAGAA GGGGTCCTCT GACAATCAGG GGACAATGGG 000320 000321 GAATTATGCT CTCCAGACTT TCTACACACA CAAGTCACAC AGGAAGGAAG GTAAAGAGAA ACTAGAGAAA ATAATTTTTG 000400 000401 AAGAAAAACA TTTCAGGAAG TATTGAAAGT ACACGGTAAC TCAGCCTGGG GCAGGGGTGG AGGGCAGCAG CACTGTTTGC 000480 000481 TGCAGCTATG CTCCTTCCTC AGTGCCCTGC ACACCCGGGA CTTGCTCGGT GAGCATCTCT CGTGTCAGTG ACAGCTAGTG 000560 000561 TGA |
