Difference between revisions of "RASSF1-AS1"
Chunlei Yu (talk | contribs) (→Expression) |
Chunlei Yu (talk | contribs) (→Expression) |
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| rowspan="3"| Cloning (overexpression and promoter assay) | | rowspan="3"| Cloning (overexpression and promoter assay) | ||
| − | | | + | | | Antisense-ANRASSF1 |
| | TGGGTACCGCAGCGGGTGGAGTACTTG | | | TGGGTACCGCAGCGGGTGGAGTACTTG | ||
| | 5'-TGAAGCTTCCAATGAGGAAAGGGGAAGT-3'<ref name="ref1" /> | | | 5'-TGAAGCTTCCAATGAGGAAAGGGGAAGT-3'<ref name="ref1" /> | ||
|- | |- | ||
| − | | | + | | | Antisense-Promoter |
| | 5'-AGATCTGACCCAGGACGCGGCAAC-3' | | | 5'-AGATCTGACCCAGGACGCGGCAAC-3' | ||
| | 5'-AAGCTTCCAGAGGCCACTCTTGTGC-3'<ref name="ref1" /> | | | 5'-AAGCTTCCAGAGGCCACTCTTGTGC-3'<ref name="ref1" /> | ||
|- | |- | ||
| − | | | + | | | Sense-Promoter |
| | 5'-AAGCTTGACCCAGGACGCGGCAAC-3' | | | 5'-AAGCTTGACCCAGGACGCGGCAAC-3' | ||
| | 5'-AGATCTCCAGAGGCCACTCTTGTGC-3'<ref name="ref1" /> | | | 5'-AGATCTCCAGAGGCCACTCTTGTGC-3'<ref name="ref1" /> | ||
Revision as of 04:45, 21 April 2015
RASSF1-AS1, which was transcribed from the opposite strand on the RASSF1 gene locus in 3p21.3 of 790bp, recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation.
Contents
Annotated Information
Name
RASSF1-AS1:RASSF1 antisense RNA 1(HGNC nomenclature)
ANRASSF1[1]
Characteristics
ANRASSF1, an endogenous unspliced long noncoding RNA (lncRNA) that is transcribed from the opposite strand on the RASSF1 gene locus through RNA polymerase II ,is 5′-capped and polyadenylated and exhibits nuclear localization and has a shorter half-life compared with other lncRNAs that bind PRC2[1].
It belongs to the category of "Antisense" in lncRNA classification.
Cellular Localization
It exhibits nuclear localization[1].
Function
The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation.[1].
Expression
ANRASSF1 endogenous expression is higher in breast and prostate tumor cell lines compared with non-tumor, and an opposite pattern is observed for RASSF1A[1].
ANRASSF1 overexpression causes a marked increase in both PRC2 occupancy and histone H3K27me3 repressive marks, specifically at the RASSF1A promoter region[1].
| Assay | Primer | Forward primer | Reverse primer |
|---|---|---|---|
| qPCR | ANRASSF1 | 5'-GGCAATTAGAACGCTCCTTG-3' | 5'-CTGTGCTAGGCGATAGAGATCC-3'[1] |
| RASSF1A | 5'-ACCTCTGTGGCGACTTCATC-3' | 5'-GTTCGTGTCCCGCTCCAC-3'[1] | |
| Strand specific RT | ANRASSF1 | 5'-CCGCGCAGAATTAGCCTCT-3' | 5'-CCAATGAGGAAAGGGGAAGT-3'[1] |
| Cloning (overexpression and promoter assay) | Antisense-ANRASSF1 | TGGGTACCGCAGCGGGTGGAGTACTTG | 5'-TGAAGCTTCCAATGAGGAAAGGGGAAGT-3'[1] |
| Antisense-Promoter | 5'-AGATCTGACCCAGGACGCGGCAAC-3' | 5'-AAGCTTCCAGAGGCCACTCTTGTGC-3'[1] | |
| Sense-Promoter | 5'-AAGCTTGACCCAGGACGCGGCAAC-3' | 5'-AGATCTCCAGAGGCCACTCTTGTGC-3'[1] |
Sequence
>gi|565671811|ref|NR_109831.1| Homo sapiens RASSF1 antisense RNA 1 (RASSF1-AS1), long non-coding RNA
000081 GCGGGTGCCA GCGTCCGGGC AAGCGCACAA GAGTGGCCTC TGGCCGGAGG CGAGGGCGGG AAGGTGCGGG AAGTGCGCGT 000160
000161 GCGCGGAGCC TGGGTCAGCC TGGGCCCGGG TCCGCTTGCA GCGGGTGGAG TACTTGCGGA GCCGGCAATC CAGGCTCCCC 000240
000241 TCCCAGCCCC CGCGCAGAAT TAGCCTCTCT GTGCCGCCGG GAAATCGGCA ATTAGAACGC TCCTTGCGCG CGGCACCCAG 000320
000321 GCAGCCCTCG AGAATGCCTG CACTGTGGCC TGCCCATCCT CGCCCTTCCC ATACGCCCTC GGCCCCGCGC TCACCACGTT 000400
000401 CGTGTCCCGC TCCACCGCGG GTTCCCAGCC CAGGTCCCGG GGCCCGCAAC AGTCCAGGCA GACGAGCGCG CGGCAGCGGT 000480
000481 AGTGGCAGGT GAACTTGCAA TCTGCAGAGA GGCCTGGCGG TGAGGCGGAG GAGCTCCAGG TCGGGGAAAT GTCCCGGAGA 000560
000561 TTGAAGGGAA GCCCCAGGGA GAGGGCCGCT GCTCGCCAGG CTCCGCAGGC CCGACCTATC TCAGTGGGTT ACCTCACACT 000640
000641 GCTACGCGGA CTCTAATGTT GGCCACCTGG GCGTCTGGAA ACCGGCCGGA AGGCCACAGG CAGAGAGGCC TGCTCAACAG 000720
000721 TTGGATCTCT ATCGCCTAGC ACAGAACTTC CCCTTTCCTC ATTGGCAATT AAAAAAACAA CAACAAAAAA
Labs working on this lncRNA
- Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brasil[1].
References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Beckedorff FC, Ayupe AC, Crocci-Souza R, Amaral MS, Nakaya HI, Soltys DT, et al. The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation[J]. PLoS genetics. 2013,9(8):e1003705.
