Difference between revisions of "LRP1-AS"

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(Annotated Information)
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===Function===
 
===Function===
[[File: ABALON-1.jpg|right|thumb|400px|'''Proposed model of action of ''INXS'' lncRNA''' <ref name="ref1" />.]]
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[[File: ABALON-2.jpg|right|thumb|400px|'''Proposed model of action of ''INXS'' lncRNA''' <ref name="ref1" />.]]
 
Lrp1-AS directly binds to High mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. Short oligonucleotides targeting Lrp1-AS inhibit the interaction of antisense transcript and Hmgb2 protein, and increase Lrp1 expression by enhancing Hmgb2 activity. LRP1-AS negatively regulates LRP1 gene expression through modulating the activity of the non-histone chromatin modifier HMGB2 and  LRP1-AS is elevated in the brains of AD patients where it might repress LRP1 expression. <ref name="ref1" />
 
Lrp1-AS directly binds to High mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. Short oligonucleotides targeting Lrp1-AS inhibit the interaction of antisense transcript and Hmgb2 protein, and increase Lrp1 expression by enhancing Hmgb2 activity. LRP1-AS negatively regulates LRP1 gene expression through modulating the activity of the non-histone chromatin modifier HMGB2 and  LRP1-AS is elevated in the brains of AD patients where it might repress LRP1 expression. <ref name="ref1" />
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===Disease===
 
===Disease===
 
*Alzheimer’s disease
 
*Alzheimer’s disease

Revision as of 12:29, 28 June 2016

Annotated Information

Approved Symbol

LRP1-AS

Approved Name

LRP1 antisense RNA [1]

Previous Symbols

_

Synonyms

_

Function

Proposed model of action of INXS lncRNA [1].

Lrp1-AS directly binds to High mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. Short oligonucleotides targeting Lrp1-AS inhibit the interaction of antisense transcript and Hmgb2 protein, and increase Lrp1 expression by enhancing Hmgb2 activity. LRP1-AS negatively regulates LRP1 gene expression through modulating the activity of the non-histone chromatin modifier HMGB2 and LRP1-AS is elevated in the brains of AD patients where it might repress LRP1 expression. [1]

Disease

  • Alzheimer’s disease

Expression

Depending of the total amount of RNA present in a single cell it has been calculated that one copy of a transcript per cell corresponds to RPKM value between 0.5 and 5. Thus, in the human brain, there is approximately one transcript of LRP1-AS per cell.

Primer Forward primer Reverse primer
qRT-PCR 5'-GGAAAGCTGCAGCCTTTCTT-3' 5'-GTGGCTGTGTTGAAGGATAC-3'[1]

Chromosome

12q13.3

RefSeq ID

NR_131939

OMIM ID

_

Ensembl ID

ENSG00000259125

Reference

  1. 1.0 1.1 1.2 1.3 Yamanaka, Y. et al. Antisense RNA controls LRP1 Sense transcript expression through interaction with a chromatin-associated protein, HMGB2. Cell reports 11, 967-976, doi:10.1016/j.celrep.2015.04.011 (2015).

Sequence

>gi|822092842|ref|NR_131939.1| Homo sapiens LRP1 antisense RNA (LRP1-AS), transcript variant 2, long non-coding RNA

000001 GTGCACCCCT GCTGCATGAG GCTCAGCCCC AGGGGTCCAA CAGAGGTCAC AGTCACAGCA CAGAATGGAA GGCTTGCAGG 000080
000081 GAGGGCTGCC CTGGAGGGCA GAGTGAAAGC AGGGGAAAGC TGCAGCCTTT CTTCTTCGTC CTCCTTGGGG AGCTAATTAC 000160
000161 AGGCCAGCAG CCACTGTGCA GGTGAGCTGT GGACAGCAGC AGCCCAAGGA CGCTATCAGG GATGCTCTGG AGGAGCCAAA 000240
000241 GGCAGAAATG TGAACCTTAC GCTGAGGCCA CGGAGAGTGA GGGCTCTCAG AGGAACCCCT GCTCGCCACC ATCCCGGGGA 000320
000321 GGGGCCTTTC TCTCTCCCAG AATGAATCCC TTGTAACCTC CTGGGATCTT TCTTTTCATC ACAAGAACCA AATAAAGAAA 000400
000401 CTTGAAGATC TTCTAAAAAA AAAAA