Difference between revisions of "MDC1-AS1"
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===Function=== | ===Function=== | ||
| − | MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1. MDC1-AS also exhibits significant decreases in proliferation,colony formation, migration and invasion.<ref name="ref1" /> | + | MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.<ref name="ref1" /> |
| + | MDC1-AS also exhibits significant decreases in proliferation,colony formation, migration and invasion.<ref name="ref1" /> | ||
===Diseases=== | ===Diseases=== | ||
Revision as of 12:28, 14 September 2016
Contents
Annotated Information
Approved Symbol
MDC1-AS1
Approved Name
MDC1 antisense RNA 1
Synonyms
MDC1-AS
Chromosome
6p21.33
RefSeq ID
NR_133647
Ensembl ID
ENSG00000224328
pubmed IDs
25514464
Cellular Localization
MDC1-AS primarily exists in the nuclear fraction [1].
Function
MDC1-AS acts as a tumor suppressor in bladder cancer and exerts positive regulatory effect on its neighboring coding gene MDC1.[1] MDC1-AS also exhibits significant decreases in proliferation,colony formation, migration and invasion.[1]
Diseases
MDC1-AS may act as a tumor suppressor in bladder cancer [1].
Expression
Expression levels of MDC1-AS and MDC1 in tissues are significantly correlated in a positive direction, indicating a potential regulating role of MDC1-AS on the coding gene MDC1.[1].
| Experiment | Primer | Forward primer | Reverse primer |
|---|---|---|---|
| RT-PCR | MDC1-AS | 5'-TCCCAGATGTGCCAAAGTCAG-3' | 5'-AGCAACCCCAGTTGTCATTC-3'[1] |
| MDC1 | 5'-GCAGCTTCCAGACAACAG-3' | 5'-TACCCATGACTTTATCCACA-3'[1] | |
| GAPDH | 5'-AAGGTGAAGGTCGGAGTCAAC-3' | 5'-GGGGTCATTGATGGCAACAATA-3' [1] | |
| RNAi | siMDC1 | 5'-GCGGCUACAUAUCUUUAGUTT-3' | 5'-ACUAAAGAUAUGUAGCCGCTT-3'[1] |
Labs working on this lncRNA
- Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer
Center, Nanjing Medical University, Nanjing, China
References
Sequence
>gi|949474747|ref|NR_133647.1| Homo sapiens MDC1 antisense RNA 1 (MDC1-AS1), long non-coding RNA
000081 AGGGCACACA GGAACTTGAC TGTCCGGCGG ATGCGATCAG TGACCAGACA CCTGATGCTT CTTTATATGT ATAATGGCTG 000160
000161 ACCCTGGCGG GACTTCCTTC TCCACTTGTG TGTTGATGTC CACTGTGGTG GAGGCTTGGC TTCTCTCCAG GTGGATCCCA 000240
000241 GATGTGCCAA AGTCAGCGCT GCTGAGACTT CTTCCTCGTC ATCTGTATCG CTGTTGATAA CCATGGAAGC TTGGCTTTTC 000320
000321 TCCAGAGGGA CAGCCTGTGG GGCCTTGCCT TCTTCCACAT CTGTATCACT ACCAGCCTGG CTCTCCTGCA GATGGGCCAG 000400
000401 GCCTGGTGCT CCAGGACCCC TTGTACCTAC TCCATGGAAG ATCTTCCTCT TCTTCATAGG AATGACAACT GGGGTTGCTG 000480
000481 GGATCCTCTC TTCTTCCGCA TCAGTGTCGC TGTCGATGAA GCCAAAAGGC TGAGCCCTTT CCAAATGGAC CTCAGCTGGC 000560
000561 CTTCCAGGAG GCCTGCTGTC ATCATCCACA TCTGTGTCAC TGTCCTCTCC AGGAGGTTGG CTCCTCTCCA GAATCACCCC 000640
000641 AGCTGGAACC ACCCCATTCC CTGCACCCCT CTTGACTTTT GTATCATTGT CCCTCTCCTT CACTAAAGGC TGATCCTTTT 000720
000721 CAAGCTGGAT TTCAGTTA
Predicted Small Protein
| Name | MDC1-AS1_smProtein_302:532 |
| Length | 76 |
| Molecular weight | 7927.2718 |
| Aromaticity | 0.105263157895 |
| Instability index | 48.4697368421 |
| Isoelectric point | 10.3208618164 |
| Runs | 14 |
| Runs residual | 0.0428009679371 |
| Runs probability | 0.0466033260151 |
| Amino acid sequence | MEAWLFSRGTACGALPSSTSVSLPAWLSCRWARPGAPGPLVPTPWKIFLFFIGMTTGVAG ILSSSASVSLSMKPKG |
| Secondary structure | LEEEEELLLEELLLLLLLLLLLLHHHHLLLLLLLLLLLLLLLLLLEEEEEEELLLLLHHH EEELLLEEEEEEELLL |
| PRMN | LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLHHHHHHHHHHHHHHHHHH HHLLLLLLLLLLLLLL |
| PiMo | ooooooooooooooooooooooooooooooooooooooooooTTTTTTTTTTTTTTTTTT TTiiiiiiiiiiiiii |
