Difference between revisions of "PGR-AS1"
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==Annotated Information== | ==Annotated Information== | ||
===Name=== | ===Name=== | ||
| − | PGR-AS1:PGR antisense RNA 1 | + | ''PGR-AS1'': PGR antisense RNA 1 (HGNC nomenclature), AT1, AT2, AT3 <ref name="ref1" />. |
| − | |||
===Characteristics=== | ===Characteristics=== | ||
| − | The AT2 transcripts are spliced and polyadenylated, contain repetitive elements (long and short interspersed nuclear elements (LINEs and SINEs) and long terminal repeats (LTRs)) and are transcribed over a 70-kb region of genomic DNA | + | ''PGR-AS1'' is five exon transcript of 1223 bp (GenBank) long noncoding RNA and is found on human chromosome 11q22.1. The AT2 transcripts are spliced and polyadenylated, contain repetitive elements (long and short interspersed nuclear elements (LINEs and SINEs) and long terminal repeats (LTRs)) and are transcribed over a 70-kb region of genomic DNA <ref name="ref1" />. |
===Function=== | ===Function=== | ||
| − | Antisense transcript AT2-T47D could be purified from T47D cells transfected with inhibitory agRNA PR9 biotinylated on the strand complementary to AT2-T47D. Addition of activating agRNA PR11 promots the association of AGO protein with antisense transcript AT2-MCF7<ref name="ref1" /> | + | Antisense transcript AT2-T47D could be purified from T47D cells transfected with inhibitory agRNA PR9 biotinylated on the strand complementary to AT2-T47D. Addition of activating agRNA PR11 promots the association of AGO protein with antisense transcript AT2-MCF7 <ref name="ref1" />. |
===Expression=== | ===Expression=== | ||
[[File:A2 exp.jpg|right|thumb|400px|'''Expression levels of antisense transcript AT2'''<ref name="ref1" />.]] | [[File:A2 exp.jpg|right|thumb|400px|'''Expression levels of antisense transcript AT2'''<ref name="ref1" />.]] | ||
AT2-T47D(found only in T47D cells) and AT2-MCF7(found only in MCF7 cells) antisense transcripts are the most highly expressed transcripts.<ref name="ref1" /> | AT2-T47D(found only in T47D cells) and AT2-MCF7(found only in MCF7 cells) antisense transcripts are the most highly expressed transcripts.<ref name="ref1" /> | ||
| + | |||
| + | ===Regulation=== | ||
| + | |||
| + | ===Disease=== | ||
| + | |||
| + | ==Labs working on this lncRNA== | ||
| + | *Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA. | ||
| + | |||
| + | ==References== | ||
| + | <references> | ||
| + | <ref name="ref1"> | ||
| + | Schwartz JC, Younger ST, Nguyen NB, Hardy DB, Monia BP, Corey DR, Janowski BA. | ||
| + | Antisense transcripts are targets for activating small RNAs. Nat Struct Mol Biol. | ||
| + | 2008 Aug;15(8):842-8. | ||
| + | </ref> | ||
| + | </references> | ||
===Sequence=== | ===Sequence=== | ||
| − | >NR_073144.1 Homo sapiens PGR antisense RNA 1 (PGR-AS1), antisense RNA | + | >gi|101054525|ref|NR_073144.1| Homo sapiens PGR antisense RNA 1 (PGR-AS1), antisense RNA |
<dnaseq>TGGACTCCCCTTTTCTCCTCCCCCGTCTCCAGGAGGAGGGAAAAGGGAAGGAGGAGGGGGTTTCGGGAAT | <dnaseq>TGGACTCCCCTTTTCTCCTCCCCCGTCTCCAGGAGGAGGGAAAAGGGAAGGAGGAGGGGGTTTCGGGAAT | ||
ATAGGGGCAGAGGGAGGAGAAAGTGGGTGTTGAATGTGGCTGGACCGGAGGGATCTCCACCTCCTGGGTC | ATAGGGGCAGAGGGAGGAGAAAGTGGGTGTTGAATGTGGCTGGACCGGAGGGATCTCCACCTCCTGGGTC | ||
| Line 34: | Line 49: | ||
TGGTCCCAAACTCCTGGCCTCAAGCTATCCTCCTGCCTTGACGTTCGAAAGCACTGAGATTTTCCTCTCT | TGGTCCCAAACTCCTGGCCTCAAGCTATCCTCCTGCCTTGACGTTCGAAAGCACTGAGATTTTCCTCTCT | ||
GTCCTGCCGCCATGTGAAGAAAGATGTGTTTGCTTCCCCTTCCACCGTGATTGTAATTTTCCTGCAGCCT | GTCCTGCCGCCATGTGAAGAAAGATGTGTTTGCTTCCCCTTCCACCGTGATTGTAATTTTCCTGCAGCCT | ||
| − | CCCCAGCCAAGCTGAACTGTGAGTCAATTAAAC | + | CCCCAGCCAAGCTGAACTGTGAGTCAATTAAAC</dnaseq> |
| − | </dnaseq | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
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Revision as of 07:52, 27 December 2018
Contents
Annotated Information
Name
PGR-AS1: PGR antisense RNA 1 (HGNC nomenclature), AT1, AT2, AT3 [1].
Characteristics
PGR-AS1 is five exon transcript of 1223 bp (GenBank) long noncoding RNA and is found on human chromosome 11q22.1. The AT2 transcripts are spliced and polyadenylated, contain repetitive elements (long and short interspersed nuclear elements (LINEs and SINEs) and long terminal repeats (LTRs)) and are transcribed over a 70-kb region of genomic DNA [1].
Function
Antisense transcript AT2-T47D could be purified from T47D cells transfected with inhibitory agRNA PR9 biotinylated on the strand complementary to AT2-T47D. Addition of activating agRNA PR11 promots the association of AGO protein with antisense transcript AT2-MCF7 [1].
Expression
Expression levels of antisense transcript AT2[1].
AT2-T47D(found only in T47D cells) and AT2-MCF7(found only in MCF7 cells) antisense transcripts are the most highly expressed transcripts.[1]
Regulation
Disease
Labs working on this lncRNA
- Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA.
References
Sequence
>gi|101054525|ref|NR_073144.1| Homo sapiens PGR antisense RNA 1 (PGR-AS1), antisense RNA
000001 TGGACTCCCC TTTTCTCCTC CCCCGTCTCC AGGAGGAGGG AAAAGGGAAG GAGGAGGGGG TTTCGGGAAT ATAGGGGCAG 000080
000081 AGGGAGGAGA AAGTGGGTGT TGAATGTGGC TGGACCGGAG GGATCTCCAC CTCCTGGGTC GGGGGCGGGG GAGGGCGGCG 000160
000161 CTGGTCAGCT CCTGCCCTTG GCCTCCATCC TGTCGTCAGG GGAACTGTGG CTGTCGTTTG TCCCAGCGAG CGGCAAGTGG 000240
000241 GGAGCGCAAG AAAAAGTAGT AATTGTTAGG AGATCTCGTC TCCTAACTCG GGGAGTTCTC CAAGAGAGTT CTCCAACTTC 000320
000321 TGTCCGAGGA CTGGAGACGC AGAGTACTCA CAAGTCCGGC ACTTGAGTGG CTGCGGCTGC GACGGCAATT TAGTGACACG 000400
000401 CGGCTCCTTT ATCTCCCGAC TTTTTCTCTG GCATCAAACT CGTGCATGCT GTGAAGCTCT CAGTCCCTCG CTGAGTTCCA 000480
000481 CTGCCCCCTC ACTAAAACCC TGGGGCTAGT CGGACCTCTC GGTACAGCCC ATTCCCAGGA AGGGTCGGAC TTCTGCTGGC 000560
000561 TCCGTACTGC GGGCGACAGT CATCTCCGAA GATCTCAGAT CCCAGTAGTG CGGGAGCACT AGCCGCCTCG GGTTGTAGAT 000640
000641 TTCACTCAAA TGACAAGTGA AGCTAGTTCT CATTGAGAAT GCCACCCACA CGCACAAATA CAACAAGGCT TACCCCGATT 000720
000721 AGTGACAGCT GTGGACTGGC CAGACAGCTT TCTAACAACG CCTCCTCCTC TAGGGAGGCC CCGCCCAAAG CCCCTCCCTA 000800
000801 CCCCAATTAC CGGACCTCAA GGTCTAGCTG TGCTAATGAC TCATAGTTTA TCTCAACCAT GTATAAAGAA TGCAGAAGAC 000880
000881 TCCAGAAGGT GGGGGAGCCA CTAGAGGATT CCATCCAGGA CACCACATTT AATTGTTATG ATGTCTTGTG CTCCTCTTGG 000960
000961 CTGTGAGAGT TTCTCAGATT TTCCTTGTAT TTGATGACCT TGACAGTCCT TAGGAGTACT GAGATGGGGG TTTCACGATA 001040
001041 TTGCCCAGGC TGGTCCCAAA CTCCTGGCCT CAAGCTATCC TCCTGCCTTG ACGTTCGAAA GCACTGAGAT TTTCCTCTCT 001120
001121 GTCCTGCCGC CATGTGAAGA AAGATGTGTT TGCTTCCCCT TCCACCGTGA TTGTAATTTT CCTGCAGCCT CCCCAGCCAA 001200
001201 GCTGAACTGT GAGTCAATTA AAC
000081 AGGGAGGAGA AAGTGGGTGT TGAATGTGGC TGGACCGGAG GGATCTCCAC CTCCTGGGTC GGGGGCGGGG GAGGGCGGCG 000160
000161 CTGGTCAGCT CCTGCCCTTG GCCTCCATCC TGTCGTCAGG GGAACTGTGG CTGTCGTTTG TCCCAGCGAG CGGCAAGTGG 000240
000241 GGAGCGCAAG AAAAAGTAGT AATTGTTAGG AGATCTCGTC TCCTAACTCG GGGAGTTCTC CAAGAGAGTT CTCCAACTTC 000320
000321 TGTCCGAGGA CTGGAGACGC AGAGTACTCA CAAGTCCGGC ACTTGAGTGG CTGCGGCTGC GACGGCAATT TAGTGACACG 000400
000401 CGGCTCCTTT ATCTCCCGAC TTTTTCTCTG GCATCAAACT CGTGCATGCT GTGAAGCTCT CAGTCCCTCG CTGAGTTCCA 000480
000481 CTGCCCCCTC ACTAAAACCC TGGGGCTAGT CGGACCTCTC GGTACAGCCC ATTCCCAGGA AGGGTCGGAC TTCTGCTGGC 000560
000561 TCCGTACTGC GGGCGACAGT CATCTCCGAA GATCTCAGAT CCCAGTAGTG CGGGAGCACT AGCCGCCTCG GGTTGTAGAT 000640
000641 TTCACTCAAA TGACAAGTGA AGCTAGTTCT CATTGAGAAT GCCACCCACA CGCACAAATA CAACAAGGCT TACCCCGATT 000720
000721 AGTGACAGCT GTGGACTGGC CAGACAGCTT TCTAACAACG CCTCCTCCTC TAGGGAGGCC CCGCCCAAAG CCCCTCCCTA 000800
000801 CCCCAATTAC CGGACCTCAA GGTCTAGCTG TGCTAATGAC TCATAGTTTA TCTCAACCAT GTATAAAGAA TGCAGAAGAC 000880
000881 TCCAGAAGGT GGGGGAGCCA CTAGAGGATT CCATCCAGGA CACCACATTT AATTGTTATG ATGTCTTGTG CTCCTCTTGG 000960
000961 CTGTGAGAGT TTCTCAGATT TTCCTTGTAT TTGATGACCT TGACAGTCCT TAGGAGTACT GAGATGGGGG TTTCACGATA 001040
001041 TTGCCCAGGC TGGTCCCAAA CTCCTGGCCT CAAGCTATCC TCCTGCCTTG ACGTTCGAAA GCACTGAGAT TTTCCTCTCT 001120
001121 GTCCTGCCGC CATGTGAAGA AAGATGTGTT TGCTTCCCCT TCCACCGTGA TTGTAATTTT CCTGCAGCCT CCCCAGCCAA 001200
001201 GCTGAACTGT GAGTCAATTA AAC
