Difference between revisions of "HMMR-AS1"
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HMMR-AS1 is hyperexpressed in GBM cell lines<ref name="ref2" />. | HMMR-AS1 is hyperexpressed in GBM cell lines<ref name="ref2" />. | ||
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| + | ! | Experiment | ||
| + | ! | Forward primer | ||
| + | ! | Reverse primer | ||
| + | |- | ||
| + | | rowspan="1"|Quantitative real-time PCR | ||
| + | | | 5'-AACTCGCCTATTTAGCCTGGG- 3' | ||
| + | | | 5'-ATACCAGGAACCAGGAGTTGTGT- 3'<ref name="ref2" /> | ||
| + | |} | ||
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===Disease=== | ===Disease=== | ||
Basal-like breast cancers <ref name="ref1" /> | Basal-like breast cancers <ref name="ref1" /> | ||
Latest revision as of 04:06, 8 August 2019
Contents
Annotated Information
Name
HMMR-AS1:HMMR antisense RNA 1
RP11-80G.1 [1]
LncBook transcript ID: HSALNT0097120
Characteristics
HMMR-AS1(HMMR antisense RNA 1; RP11-80G.1) is transcribed on the strand opposite to the adjacent HMMR gene which codes for receptor for hyaluronan-mediated motility (RHAMM), a mitotic spindle-binding protein [1].
Function
Effects of HMMR-AS1 knockdown on the proliferation of MDA-MB-231 and MDA-MB-468 cells[1].
Effects of HMMR-AS1 knockdown on the migration ability of MDA-MB-231 and MDA-MB-468 cells[1].
- HMMR-AS1 influences the progression of BLBCs (Basal-like breast cancers) through regulating HMMR [1]. In MDA-MB-231 and MDA-MB-468 breast cancer cells, the proliferation and migration abilities were remarkably suppressed via knocking down HMMR-AS1 in vitro [1].
- HMMR-AS1 stabilizes HMMR mRNA. Knockdown of HMMR-AS1 reduces HMMR expression; inhibits cell migration, invasion, and mesenchymal phenotypes; and suppresses GBM cell growth both in vitro and in vivo. Moreover, knockdown of HMMR-AS1 radiosensitizes GBM by reducing DNA repair proteins ATM, RAD51, and BMI1[2].
Expression
The expression of HMMR-AS1 is markedly higher in BLBCs in contrast to those in non-BLBC subtypes [1]. Besides, the expression levels of HMMR-AS1 and HMMR in the tumor tissue samples are positively correlated (R=0.802, P<0.0001) [1].
HMMR-AS1 is hyperexpressed in GBM cell lines[2].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| Quantitative real-time PCR | 5'-AACTCGCCTATTTAGCCTGGG- 3' | 5'-ATACCAGGAACCAGGAGTTGTGT- 3'[2] |
Disease
Basal-like breast cancers [1]
Glioblastoma[2]
Sequence
>NR_109892.1 Homo sapiens HMMR antisense RNA 1 (HMMR-AS1), long non-coding RNA
000001 AGTCAGCAGC TGACAGAATA ATCCACACAT TGGTTTCCTT ACCTATGGAG TGAGGGCTAT TATGGTGGAA AAGGCCAAGT 000080
000081 AGAAGCCACT AGAACTATCT GTACTTAGGA AAATAGTAAA CCAAAAGCAA TGCTCAATTC CTATAGGGAC TGAAGAGATT 000160
000161 AGTGCCACCA TAAGTACTTG AAGGAAGGCG GGGTGGTGAT TCCTACCATA TGTCCATTCA ACTCGCCTAT TTAGCCTGGG 000240
000241 CAGAAGACAG ATGGATTATC ACAAACTTAA CTAGGTGGTG ACTCCAATTG CAGCTACAGT ACCAGATGTA TTGCTTGAGC 000320
000321 AAATTAACAC AACTCCTGGT TCCTGGTATG CAGCTACCTG GAATCTTGAT TGCCTTTTCT TTCCACATCA CATTGGTCCA 000400
000401 TTACATTGAC GACATTATGC TCACTGGACC TAATGAGTGA GACATAGCAA CTACTCCAGA CCTATTGGAA CTGACTTCAC 000480
000481 CTGCAACAGA GCTTGGAGAA GCTGAAGCCT AAGGGTGCTC TAAAGCAGGG GTCCCCAACC TCCGGGCTAC AGACCAATAC 000560
000561 CCATCCAAGG CCTGTTAGGA ACCCGGCTGC AGAGCAGGAG CTTTTCTGCA TGGAAGAATT AAATTGAGAA AAACATAGTT 000640
000641 ATTTAAAAAA TCACATCGTA ACTGAGTTAA CAAGGCACTC AGGTTCTAAT CATAGATTAC CTTCCTTCTT CATCTTCCAG 000720
000721 CTGTTTTCTA AAGTCTTCCT CCTGTTGCTT GAGTTGGTTC TGCAAATCAG TTATTTTTTG AAGAAAAGAA ACTGTGATTT 000800
000801 CTTTAATTTC TGTTTCCTTT AGTGCTGACT TGGTCTGCAG ATCTAGAAGC ATCCTTTGGT TTGAGAGAGA AGAGGTCACT 000880
000881 AAACATTTTA TAGCCACTTT CATTAATTTT TCCTTTTTTT TTCTTTAAAC CTCAAGGAAC TCTTCAAGAA GTGTCATTTT 000960
000961 TTAACTGAGT AAATTCTTAA TTTGCAGTAT AAGATGGCCA AAAGCAAGCC AAGGGACAGT TACTAGCTGG CTTTCAACAT 001040
001041 TAAATTGTCT TAATGGTTCT AAGGCCATTA TTTGCTCTAT ATACCTTACA TATTCTTGAT TTGAGCTCTC AGTTGCCAAA 001120
001121 ATCTGATGCT GAACATCCTC TGCATTTTTC CCAGCCTTGG CCGCTTTTTC CTGTAATGAT GAGTTCTCCA GCTTAAGATC 001200
001201 TTCTATCTCA CTGGCTGTTA ACGCTTTATA GCTTATTAAA AAGAAAAAAA AGTCAAAGTA GTTTTCTAA
000081 AGAAGCCACT AGAACTATCT GTACTTAGGA AAATAGTAAA CCAAAAGCAA TGCTCAATTC CTATAGGGAC TGAAGAGATT 000160
000161 AGTGCCACCA TAAGTACTTG AAGGAAGGCG GGGTGGTGAT TCCTACCATA TGTCCATTCA ACTCGCCTAT TTAGCCTGGG 000240
000241 CAGAAGACAG ATGGATTATC ACAAACTTAA CTAGGTGGTG ACTCCAATTG CAGCTACAGT ACCAGATGTA TTGCTTGAGC 000320
000321 AAATTAACAC AACTCCTGGT TCCTGGTATG CAGCTACCTG GAATCTTGAT TGCCTTTTCT TTCCACATCA CATTGGTCCA 000400
000401 TTACATTGAC GACATTATGC TCACTGGACC TAATGAGTGA GACATAGCAA CTACTCCAGA CCTATTGGAA CTGACTTCAC 000480
000481 CTGCAACAGA GCTTGGAGAA GCTGAAGCCT AAGGGTGCTC TAAAGCAGGG GTCCCCAACC TCCGGGCTAC AGACCAATAC 000560
000561 CCATCCAAGG CCTGTTAGGA ACCCGGCTGC AGAGCAGGAG CTTTTCTGCA TGGAAGAATT AAATTGAGAA AAACATAGTT 000640
000641 ATTTAAAAAA TCACATCGTA ACTGAGTTAA CAAGGCACTC AGGTTCTAAT CATAGATTAC CTTCCTTCTT CATCTTCCAG 000720
000721 CTGTTTTCTA AAGTCTTCCT CCTGTTGCTT GAGTTGGTTC TGCAAATCAG TTATTTTTTG AAGAAAAGAA ACTGTGATTT 000800
000801 CTTTAATTTC TGTTTCCTTT AGTGCTGACT TGGTCTGCAG ATCTAGAAGC ATCCTTTGGT TTGAGAGAGA AGAGGTCACT 000880
000881 AAACATTTTA TAGCCACTTT CATTAATTTT TCCTTTTTTT TTCTTTAAAC CTCAAGGAAC TCTTCAAGAA GTGTCATTTT 000960
000961 TTAACTGAGT AAATTCTTAA TTTGCAGTAT AAGATGGCCA AAAGCAAGCC AAGGGACAGT TACTAGCTGG CTTTCAACAT 001040
001041 TAAATTGTCT TAATGGTTCT AAGGCCATTA TTTGCTCTAT ATACCTTACA TATTCTTGAT TTGAGCTCTC AGTTGCCAAA 001120
001121 ATCTGATGCT GAACATCCTC TGCATTTTTC CCAGCCTTGG CCGCTTTTTC CTGTAATGAT GAGTTCTCCA GCTTAAGATC 001200
001201 TTCTATCTCA CTGGCTGTTA ACGCTTTATA GCTTATTAAA AAGAAAAAAA AGTCAAAGTA GTTTTCTAA
Labs working on this lncRNA
- Department of Radiation Oncology, Anhui Provincial Hospital, Hefei, People's Republic of China.
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Liu W, Ma J, Cheng Y, Zhang H, Luo W, Zhang H. HMMR antisense RNA 1, a novel long noncoding RNA, regulates the progression of basal-like breast cancer cells. Breast Cancer (Dove Med Press). 2016 Nov 24;8:223-229.
- ↑ 2.0 2.1 2.2 2.3 Li J, Ji X, Wang H. Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma. Neoplasia. 2018 May;20(5):456-466.
