Difference between revisions of "NONHSAT119717"

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[[Category:Intergenic]]
 
[[Category:Intergenic]]
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{{lncrnadb|
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tID = NONHSAT119717|
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ltID = HOTTIP|
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ann = <tab class=wikitable sep=tab head=top>
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Section Description
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ID HOTTIP
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Characteristics Gene located at 5°Ø end of HoxA cluster, bidirectional transcript with HOXA13 [http://www.ncbi.nlm.nih.gov/pubmed/17445766 (Sasaki (2007))]. ~3.8kb, transcript in human, spliced and polyadenylated [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. ~2.3kb transcript in mouse, different splice isoforms, currently known transcripts initiate downstream from the syntenic site in human.
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Expression Shows collinear regulation of expression like HOX protein coding genes [http://www.ncbi.nlm.nih.gov/pubmed/17445766 (Sasaki (2007))]. Has bivalent chromatin marks (both H3 K4me3 and H3 K27me3) whether expression off or on, but only expressed in more distal cells [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. Has a similar expression profile to HOXA13, expressed in colon, prostate, placenta and uterus, as well as hand, foreskin and foot fibroblasts ([http://www.ncbi.nlm.nih.gov/pubmed/17451645 Sasaki (2007)], [http://www.ncbi.nlm.nih.gov/pubmed/17445766 Sasaki (2007)], [http://www.ncbi.nlm.nih.gov/pubmed/21423168 Wang (2011)]). Mouse E13.5 in-situs gave similar results [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. Nuclear localised, generally to a single small nuclear spot [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. Lowly expressed, estimated at ~0.3 copies/cell in expressing tissues [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))].
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Function Summary:. Controls activation of 5°Ø HOXA genes. Chromosomal lopping bring it into close association with 5°Ø gene loci. HOTTIP binds WDR5 and recruits a MLL chromatin modifiying complex to maintain a domain of active chromatin over the 5°Ø of HOXA cluster [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. - HOTTIP controls activation of 5°Ø HOXA genes in mammals and chicken. - Impact on HOXA genes decreases with increased genomic distance from HOTTIP. - Phenotypic impact in chicken forelimb of HOTTIP knockdown was shorter than normal distal bones. - HOTTIP doesn°Øt affect chromosomal structure and looping but it was necessary for maintenance of H3 K4me3 chromatin marks over the distally expressed 5°Ø half of the HOXA locus. HOTTIP RNA binds WDR5 a component of MLL/Trithorax complexes recruiting a MLL complex to lay down H3 K4me3 marks. - WDR5 binding mediated by sequence(s) in 5°Ø 1kb of HOTTIP. HOTTIP appears to act in cis, its function is facilitated by the chromosomal looping that brings it into close proximity with 5°Ø HOXA genes.
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Conservation Present in human and mouse. Portions of HOXA13as sequence are well conserved throughout mammals [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. Appears to be present in chicken although there is little homology [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. RNA contains some highly conserved sequences which are predicted to form a conserved structure [http://www.ncbi.nlm.nih.gov/pubmed/17445766 (Sasaki (2007))].
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Misc Background: Hox genes are found in 4 clusters. Genes in each cluster are expressed in certain tissues along body axes dependent on their position in the cluster. In the HoxA cluster genes near the 5°Ø are expressed in more distal tissues while genes near the 3°Ø are expressed in more proximal tissues. Expressed genes have active chromatin marks and show close physical association (likely due to chromosomal looping). Inactive genes do not associate and have repressive chromatin marks [http://www.ncbi.nlm.nih.gov/pubmed/21423168 (Wang (2011))]. Human clone accession: GU724873. Mouse clone accession: BC046496
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Name HOTTIP - HOXA transcript at the distal tip
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</tab>|
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}}

Revision as of 11:52, 6 October 2014

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Annotated Information

Transcriptomic Nomeclature

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Function

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Regulation

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Expression

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Allelic Information and Variation

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Evolution

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Basic Information

Transcript ID

NONHSAT119717

Source

NONCODE4.0

Same with

,

Classification

intergenic

Length

3764 nt

Genomic location

chr7+:27240040..27246878

Exon number

4

Exons

27240040..27240140,27240242..27241306,27241461..27242257,27244331..27246878

Genome context

Sequence
000001 CCCTTCCTTT ATCCCAGTGG GGCCCAGACC CGCGCAACCA GGCGGGGAGG GGAGGTGGGC GCGCGATTGG GTTGCGATCT 000080
000081 GGAGCAGTGG GGACAGGTCA GGAACCGGCG CGTATTTCTG CAGTGAGACC ACAGGACGGA CATCGGCGCC TTCGGCTTCG 000160
000161 ATGGAGTTGC GATTTTGCTC TTTCCAGGGA AACAGTGGCA GGGTGTTTGC TGCTTATCGG TTCCTGCGGA TATGCCTGGG 000240
000241 TCCCAGGACA TTCCACTGGA GGCTTGGACT GCATTTAGGA GCCCCTATCC CTTCCCTGTC CACACTGTTA GTGAGCAATT 000320
000321 TCATATGTTT GCATTTAGAC CCATAGACTC AGAACGACTC ATCACACACA CACACAGTGT ACACTGACAC ACTCACATTC 000400
000401 GCACACTTAG GTATACAGCC TGATCCTTGC TCTGACCTGG TAACAACGCT TCCTCCTCCA GAGACTTTGA GATAGAGCGA 000480
000481 GCGATCCCTG TGCACCATTC ATCCATGCTC CCACCTCGCC AGTATGGCTG GCTTAGTTCT GGAAGGGGCT TAAGAGGAAC 000560
000561 AAGCCCCAGC TGTGCTTCTG GCTGGGACTT AAACCCCCCT TCTGGGCCCT AAAGCCACGC TTCTTTGTGG ACCGGACCTG 000640
000641 ACTCTCCAGG AATCTGGGAA CCCGCTATTT CACTCTATTT TGGGACAAGA AAAAGGGGCT CTTTGGGGCC ACTTCCTGCC 000720
000721 TTCCCCTCAA GTAGGATCTC CAGCCTGCAG AGGGTGCCTA GTCCTTCTTT GCCCAAGAAC CAGTCCAAGA AGCCTTTCCT 000800
000801 CTGTGCCTGG GAAATGCAAC CTTTTCTTGG GAGCATGGTA GGGTGTTGGT GCTGAAGAAC CAAGCAGCGA CCCGTCTTGT 000880
000881 AGCTGCCATG TTTTGTCGAG GGGTTCTGGG GGTCCTGCTG CTTTAGAGCC ACATACTTCC ACTTCCTGAT TCACTACTGT 000960
000961 GAGCTGGTCA GATGCCTAAG GAACAAGCGT TCAAAGTGAA AGTGGGCACA TTACCGGAAT AGTGCTGGGG AGAGTGCTGG 001040
001041 ATTCTTTTCC ACCCCAGGCG GACTGGTGAG AAGCCAGGCT TGGACCTGTC CTCTGCTCCT AGCTTGCACA CTCAGCCCTA 001120
001121 AACTCAGAGC AGCACGCATA CCACCCCTCA CACACACCCC ACCAAGTGTG CCCTTTCCTT CTTGACTTTT TAGCAGTTCT 001200
001201 GGGTAAATAT TGATTTGCCC CCAGTTTACC TTCTCCCTGA CTGGCCATTT GCAGACTCAG GAACTAGCCT CTGTAGGGAC 001280
001281 TTGATTTTTC TGTTACTTTC TGGCCGTTTC ACCACCCCCC TTCCTCCCTC CAAGTGGCAT TGTAAAACTC ACAGTGACAA 001360
001361 AGAGACAGAG TAGGGTTCTA GGCCCCTGTT CCTGGGGACT TGAAGGCGGT TTTACATACT GGTCAGACAC GGCTGGAGGC 001440
001441 CAAGGTCAAG TTGAAAGTTG CAGTCCAGCC AGCATGAGAA CTGCCATGCG AGCGTAGAGA CACAGGCAGC AGCAAAAGGC 001520
001521 CCATTGCCCA CATCCCCTCA CTCTTAATTT TCTCTCTCTT TTTAAAATTC TCGCCTCTGA CTCTGTTCGG CTGCCCAGAA 001600
001601 TTTTTTGGTG CCTTCGTGGG GTTTTTGGGG CGGTGTTTAC CGACTCTTCT CTGCCTCCGC CCTGCTCAGC CAGGGCTTTG 001680
001681 AGCCTCTTCG GTTTTCCGGC CAGACCCGGA AAAACGAAAA CACAGCTTGG GGAGCCCCCA CTAGCCGGCG CCTGTGCCAG 001760
001761 CTCACCTCTG GCCATGGCGC AGCTGCCGGT GCACACGGCG GCCAAGGCCA GCTCCACATT CTTCCCTCCC CCTCCCACTT 001840
001841 CACCGTAGCC CCGAACCCTG CGCGCAGAGA AAGGGTCTCA GCTCCACAGA CGACTGGGTC CCTCCTCACC AAAAATGGTG 001920
001921 AGACAAGATT TCATCTGTCG GCCGAGGAGC CACAAGCAGG TACCACAAAG CCACTAGTGC ACAGGGACTC AGAAAAGACG 002000
002001 GCAGGAGCCC AAGGAAAACT CCAATTTGAG TACAGCCCTG CCTTGTTTCC CCCAGAGAGT CCCTGAGCAA GGAGACCTCC 002080
002081 ACCCCACACA CACCATTTCA GAACAACCAG GTTCCAGACT CCCATGAGGA GCATCTCCCA CTGCAGAGCC TTGGCCAGCC 002160
002161 GCGCCCGGAC TCCTCAGAGC TGGCGCAAAC TCCGTCCTCC AAAACTCGGC TCTGGGAGGC CTAAGTGACT CCGAAGCCGG 002240
002241 CGGCAGCCGC GGCAGCGGCC GTGGTGGTGG AAGAGCTCTT TTCCCCGACA GTGCCACTGA TCGCTCTTCA CTGGAGCTGG 002320
002321 AAACAGCCTT CGCGGAAAGG ACCGGAGCAT GCGTTAGAAG CAGAGGGAGC TTGGTGAAGG GCTCGGCTGG AAGGAGGAAA 002400
002401 CGCCTTCTCG CAGTGCGCGG CCAGCCCGCG GGGGACACCG GCTTGCTGGA CTGCAGGGGC CCGTGCCACC CAGGAAGTGA 002480
002481 CCTGCGGGTC ACTCAGCCGG GGCGCTGGGC GAGCGCGGGA CGGCCCGGAG AATTCCGTGC GGCTGCGACG GGAAAAGGAC 002560
002561 GAGGGGTCTC TGTACCCGAC GCTGCCACTG GCCCAAAGGA ATTTTACCCG CGAGCGCCCA CCCCACCCTA GCTTGATGCT 002640
002641 TACGCCCGCA ACAAAACAGG AAACCAGGAC TGGGCAGTGC ATTCTTTAAG TCAACAAATA CACTGAAGAC TTCGAGCGTT 002720
002721 TGAAGGAAGG AGGGGGTTTG CACGTAAGCC TGGCCCCGCC GGGCTCGGCT TTCTCGCTGA GAAAGCGGCG CAGGCAGCCA 002800
002801 GGCGGCCTGG GCCCGCGGGG GTCCATCTCG CCCTAGACTC CTAAGAACTC CCACGGCCCT GTTCCCAGCT GCGAATTCTT 002880
002881 AATGCACAAC GCGACGGAGG GAAGGAAATT CACCAGCGCA GCGACGAGGA AGGGGAACTC AGGACCCCTT CAAGTACACA 002960
002961 CTGAGGTGTG ATCAGAGTTT TATGGGCACT TTATATGCTG TAATCATAAC GATGTGTGTG CCTTGATATG CACGCATATT 003040
003041 CACGCATCAA ACGTGCATAC ACACACAGAG TGAATGTGCG CATCCAATGT CATGTGGGTG AAATACAAGC ATCATACCCA 003120
003121 GCCCTACGAA AAAAAAATTC ACCCTGTCGG ACCAGGCTGG TGACATACTT CGCTGGCGCA TCTCCTTACT CACTCTTACT 003200
003201 TTTCCGACCC CTCACCATTC CCTCTCCTGT GGCTTGGTAA ATACACCTGC CCTCCGTGGA AGGTGAGTCC TGGACTGGCG 003280
003281 TTGCCAGGTT CGCATGTCCT CCCCAGAACC TCCGTCTGGC TCCAGGGACT CTCACTGAGC GGGTCTAGAG CACCCAGCAC 003360
003361 TTTTCAAGGA ACAGCCGCGG TTCCTTTGTC CCGCGGCTCC AGCCCCGTTC GGCCCAGCTC TCAGGGAAAC GAAGCGCTCA 003440
003441 GTAAGAACTT TTGATATTAG TTTGTATGGG TATTTACACT CTGGTGAGGG GAGCTGAGTA CGGAAGTTCC ATTAATCATA 003520
003521 CTCCAACCTT GGGTTTAGAT ATTCAGTTTA TGGGTTGGGA GAGGGAGTTT GCCGGAAAGA AAGCATCAAG GTTGGCCGCT 003600
003601 GACTCCAGAG AAATGAAAAG GGAGGTCGTT TTCTGTTTCT GGAAATCAAG AATTAGGAAT GGGCAACTAC AGGTGCTAAC 003680
003681 CAACAGACCA CTTTTTTGTT TTTTGGTAGC CCTTTGGCAG GGATAGTTTT TCCACCTTTG CCCGATACAA TTTAAAAAAA 003760
003761 AAAA
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Annotation (From lncRNAdb)

[NONHSAT119717]
Section Description
ID HOTTIP
Characteristics Gene located at 5°Ø end of HoxA cluster, bidirectional transcript with HOXA13 (Sasaki (2007)). ~3.8kb, transcript in human, spliced and polyadenylated (Wang (2011)). ~2.3kb transcript in mouse, different splice isoforms, currently known transcripts initiate downstream from the syntenic site in human.
Expression Shows collinear regulation of expression like HOX protein coding genes (Sasaki (2007)). Has bivalent chromatin marks (both H3 K4me3 and H3 K27me3) whether expression off or on, but only expressed in more distal cells (Wang (2011)). Has a similar expression profile to HOXA13, expressed in colon, prostate, placenta and uterus, as well as hand, foreskin and foot fibroblasts (Sasaki (2007), Sasaki (2007), Wang (2011)). Mouse E13.5 in-situs gave similar results (Wang (2011)). Nuclear localised, generally to a single small nuclear spot (Wang (2011)). Lowly expressed, estimated at ~0.3 copies/cell in expressing tissues (Wang (2011)).
Function Summary:. Controls activation of 5°Ø HOXA genes. Chromosomal lopping bring it into close association with 5°Ø gene loci. HOTTIP binds WDR5 and recruits a MLL chromatin modifiying complex to maintain a domain of active chromatin over the 5°Ø of HOXA cluster (Wang (2011)). - HOTTIP controls activation of 5°Ø HOXA genes in mammals and chicken. - Impact on HOXA genes decreases with increased genomic distance from HOTTIP. - Phenotypic impact in chicken forelimb of HOTTIP knockdown was shorter than normal distal bones. - HOTTIP doesn°Øt affect chromosomal structure and looping but it was necessary for maintenance of H3 K4me3 chromatin marks over the distally expressed 5°Ø half of the HOXA locus. HOTTIP RNA binds WDR5 a component of MLL/Trithorax complexes recruiting a MLL complex to lay down H3 K4me3 marks. - WDR5 binding mediated by sequence(s) in 5°Ø 1kb of HOTTIP. HOTTIP appears to act in cis, its function is facilitated by the chromosomal looping that brings it into close proximity with 5°Ø HOXA genes.
Conservation Present in human and mouse. Portions of HOXA13as sequence are well conserved throughout mammals (Wang (2011)). Appears to be present in chicken although there is little homology (Wang (2011)). RNA contains some highly conserved sequences which are predicted to form a conserved structure (Sasaki (2007)).
Misc Background: Hox genes are found in 4 clusters. Genes in each cluster are expressed in certain tissues along body axes dependent on their position in the cluster. In the HoxA cluster genes near the 5°Ø are expressed in more distal tissues while genes near the 3°Ø are expressed in more proximal tissues. Expressed genes have active chromatin marks and show close physical association (likely due to chromosomal looping). Inactive genes do not associate and have repressive chromatin marks (Wang (2011)). Human clone accession: GU724873. Mouse clone accession: BC046496
Name HOTTIP - HOXA transcript at the distal tip

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