Lnc-HEATR6-1:4
WFDC21P, locus in 17q23.1 and spanning 621 bp in length, bounds directly to STAT3 in the cytoplasm and regulates DC differentiation.
Contents
Annotated Information
Transcriptomic Nomeclature
WFDC21P: WAP four-disulfide core domain 21, pseudogene(HGNC nomenclature)
lnc-DC[1]
Characteristics
lnc-DC has two transcript variants(417 and 397 nucleotides), of which the 417-nt variant was expressed more highly[1].
lnc-DC has poly A tail and 5’cap structure but is without coding capacity[1].
A canonical binding motif(+44 to 50 nt relative to TSS) for the transcription factor PU.1-a key regulator of DC differentiation was found in lnc-DC promoter region[1].
Cellular Localization
- Cytoplasm[1]
Function
lnc-DC bounds directly to STAT3 in the cytoplasm which promotes STAT3 phosphorylation on tyrosine-705 by preventing STAT3 bing to and dephosphorylation by SHP1[1].
lnc-DC is vital for DC differentiation in both human and mice and function by directly interacting with signaling molecules in the cytoplasm and regulating their posttranslational modification[1]. .
Regulation
PU.1 directs lnc-DC expression in human cDCs[1].
Diseases
- DC dysfunction[1]
Expression
lnc-DC is exclusively expressed in cDCs of the hematopoietic system[1].
Knockdown and Overexpression:
| Primer | Sequences |
|---|---|
| RNAi-1 | 5'- GAG TTA TCT TAA GGA TCA T -3'[1] |
| RNAi-2 | 5'- GGA GTT CCT TGA CTA GG -3'[1] |
| scrambled control | 5'- TTC TCC GAA CGT GTC ACG T-3'[1] |
Labs working on this lncRNA
- National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China[1].
References
Basic Information
| Transcript ID |
lnc-HEATR6-1:4 |
| Source |
LNCipedia2.1 |
| Same with |
NONHSAT055064, WFDC21P, lnc-DC |
| Classification |
intergenic |
| Length |
621 nt |
| Genomic location |
chr17-:58160927..58165828 |
| Exon number |
3 |
| Exons |
58160927..58161127,58162416..58162564,58165558..58165828 |
| Genome context |
|
| Sequence |
000001 AGGACCCAGG GTACACAGGG TGGGTGGCTA TTCTCCAGAA ATGTCAGTTT CTGGGCAGGG CTTAGGTGTC TGCAGTCCCT 000080
000081 AGTCCCACCC CTGGCCTTGC ATTCCAGCTC AGCGGGTGGA AGGTATAAAT TTCAGCTGCT CTCAGCCCTG CTGTGTTTTT 000160 000161 CCAAAGCCTT CCAACAGCAA CATGAAGTTG GCAGCCTTCC TCCTCCTGTG ATCCTCATCA TCTTCAGCCT AGAGGTACAA 000240 000241 GAGCTTCAGG CTGCAGGAGA CCGGCTTTTG GGTACCTGCG TCGAGCTCTG CACAGGTGAC TGGGACTGCA ACCCCGGAGA 000320 000321 CCACTGTGTC AGCAATGGGT GTGGCCATGA GTGTGTTGCA GGGTAAGGAC AGGTAAAAAC ACCAGGCCCT CCCTGCTTTC 000400 000401 TGAAACGTTG TTCAGTCTAG ATGAAGAGTT ATCTTAAGGA TCATCTTTCC CTAAGATCGT CATCCCTTCC TGGAGTTCCT 000480 000481 ATCTTCCAAG ATGTGACTGT CTGGAGTTCC TTGACTAGGA AGATGGATGA AAACAGCAAG CCTGTGGATG GAGACTACAG 000560 000561 GGGATATGGG AGGCAGGGAA GAGGGGTTGT TTCTTTTAAT AAATCATCAT TGTTAAAAGC A |
