DGCR5

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DGCR5, is a long noncoding RNA involved in DiGeorge syndrome

Annotated Information

Approved Symbol

DGCR5

Apprroved Name

DGCR5: (DiGeorge syndrome critical region gene 5 (non-protein coding)) NCRNA00037, LINC00037 [1]

Characteristics

In humans, DGCR5 is located on chromosome 22q11, at the ADU breakpoint [2]. DGCR5 originates from a cis-acting transcriptional control element in the vicinity of the ADU/VDU breakpoint.[3]

Function

DGCR5 is a functional RNA; production of DGCR5 transcripts is necessary for regional transcriptional control.[3] Production of DGCR5 transcripts is necessary for regional transcriptional control and DGCR5 transcripts are merely a reflection of a particular chromatin configuration maintained over the region encoding the gene.[3].

Long non-coding RNA DGCR5 is involved in the regulation of proliferation, migration and invasion of lung cancer by targeting miR-1180 [4].

DGCR5 promotes the lung adenocarcinoma (LUAD) progression via playing anti-apoptosis roles. Mechanically, it exerts its biological functions through regulating miRNA miR-22-3p in a ceRNA manner[5].

Regulation

Expression of DGCR5 is regulated by the transcription factor REST. [6]

Disease

  • Clear cell renal cell carcinoma [3]
  • Digeorge syndrome [3]
  • Clear cell renal cell carcinoma [7]
  • Lung cancer susceptibility [4]
  • Velocardiofacial syndrome [8]
  • Lung adenocarcinoma (LUAD)[5]

Expression

DGCR5 inhibited the expression of AKT, GSK-3β, and β-catenin by targeting miR-1180 [4] DGCR5 is expressed during human and murine embryogenesis [3]

DGCR5 was upregulated in LUAD tissues and LUAD cell lines. Inhibition of DGCR5 can prevent LUAD progression via playing anti-apoptosis roles. Both mRNA expression and protein levels of BCL-2 were increased by DGCR5 downregulation while reversely BAX was increased[5]

Experiment Forward primer Reverse primer
Quantitative real-time PCR 5'-CACGAGTGTAGTGCCCAGTT- 3' 5'-GGTCAGGGACCTTTGTCGTT- 3'[5]

Labs working on this lncRNA

  • Molecular Medicine Unit, Institute of Child Health, London
  • Department of Pulmonary Medicine, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou 310016, Zhejiang Province, China,
  • Department of Hepatobiliary and Pancreatic Surgery, Huai’an First People’s Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, People’s Republic of China
  • Molecular Medicine Unit, Institute of Child Health, London

References

  1. ↑ Huang R, Wang X, Zhang W, et al. Down-regulation of LncRNA DGCR5 correlates with poor prognosis in hepatocellular carcinoma[J]. Cellular Physiology and Biochemistry, 2016, 40(3-4): 707-715.
  2. ↑ Sutherland HF, Wadey R, McKie JM, Taylor C, Atif U, Johnstone KA, Halford S, Kim UJ, Goodship J, Baldini A, Scambler PJ (Jul 1996). "Identification of a novel transcript disrupted by a balanced translocation associated with DiGeorge syndrome". American Journal of Human Genetics. 59 (1): 23–31.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Sutherland HF, Wadey R, McKie JM, Taylor C, Atif U, Johnstone KA, Halford S, Kim UJ, Goodship J, Baldini A,Scambler PJ. Identification of a novel transcript disrupted by a balanced translocation associated with DiGeorge syndrome. Am J HumGenet. 1996 Jul;59(1):23-31.
  4. ↑ 4.0 4.1 4.2 Chen EG, Zhang JS, Xu S, Zhu XJ & Hu HH. Long non-coding RNA DGCR5 is involved in the regulation of proliferation, migration and invasion of lung cancer by targeting miR-1180[J]. Am J Cancer Res. 2017
  5. ↑ 5.0 5.1 5.2 5.3 Dong H X , Wang R , Zeng J , et al. LncRNA DGCR5 promotes lung adenocarcinoma (LUAD) progression via inhibiting hsa-mir-22-3p[J]. Journal of Cellular Physiology, 2017, 233(29).
  6. ↑ Johnson R, Teh CH-L, Jia H, Vanisri RR, Pandey T, Lu Z-H et al. Regulation of neural macroRNAs by the transcriptional repressor REST[J]. Rna. 2009, 15(1):85-96.
  7. ↑ Yu G, Yao W, Wang J, et al. LncRNAs expression signatures of renal clear cell carcinoma revealed by microarray[J]. PloS one, 2012, 7(8): e42377.
  8. ↑ Qureshi IA, Mattick JS & Mehler MF. Long non-coding RNAs in nervous system function and disease[J]. Brain Res. 2010.

Sequence

>gi|345842381|ref|NR_002733.2| Homo sapiens DiGeorge syndrome critical region gene 5 (non-protein coding) (DGCR5), transcript variant 1, non-coding RNA

000001 GCGCGGAGCC TAGACCCGCT GCCGGGCCGG GGCTGGGCCG GAGCGGCGGC GCCTGGATGC CGGCCCGGGG CTGGGCCGGA 000080
000081 GACTGGCGCC CGCCCCGAAG CGACTTTCGG TCCCCGACCC GCTCCGCCTG ACCCCTATGA TGAAGAGGGC GTCCACTGGA 000160
000161 GTTCTTGCTG TCGACATCTT TGACAAGTTC AACTTCACGG GAGCCAGAGT CCCATGGTTC GACGCCATTC ATGAAGAATT 000240
000241 CCCCAGGGAG CTGGAGTCCT CCATCTCCTT CCCAGCCAAC TTCTTCAAAC CACCTGAAGA AAAAGAAGGC CCCCTGGTAA 000320
000321 GGCCAGCCAG CCGGAGGACC ACCCCGCAGA CCACACGCCC AGGGCCTGGC ACCGAGGGGG AGGCCCCGAT CAGCAGGTGA 000400
000401 AAGCGACACC CTGATGACAC TGGCTAGTTC ACCTTCACTC TGGTCATCGT TTACTGTACC CCGGGGCAGC TCCCGCCTGA 000480
000481 GCCCTATGAC CCCAACCGCC GCAGCCTCTG GCTGCCTCAC TGGCCCATCA TTAATACGTC GATGGTGAGC GCACTGGTGT 000560
000561 ACAGCGAGGG GGCTCCACTT CCGAGACCCC TGGAGAGGCC CGTCCTGGTG GAGTTCACCC TGCTGGAGAT GGAGAAGCGA 000640
000641 ACCAAGCCTG TCTGTGTGTT CTGGAACCAT TCCCTGGCGT AAATGTGATG CCGGGAGTTC TGCATCTTCA TGTGAGGGCT 000720
000721 TTGCCAAGAC ACAGAGAACC CGCAGGGTTG TTGGCTCAGC GGCAGATCGT CCCACGGGGA CAGTCTCTTG TGGTCTGTGT 000800
000801 CTCCCCCTTC AGCCCCCTGT GGGGCAGTGA GCCTCCTGCT CTGCTTCTGT GGGTGCATCA GAGCCCCCCC AGATGCCCAC 000880
000881 ACGCAGACGC GCACAGGCCT GCACGTGGAA ACACAGACAT GCGCACACGC AGGCCCACAC ACAGACGCAC ACAGAGCTGC 000960
000961 ACGTGGAGAC ACGTGCGCAC ACGCAGGCCC ACACGCAGGC CCACACACAG GTGCACGCGG ACCTGCACGT GGAGACACAG 001040
001041 ATGTGTGCAC ATGCAGGCCC ACACACAGGT GAGCGTGGAC CTGTGTGAAC AGATGCGCGC AGACCTGCAC GTGGAGACAC 001120
001121 AGACATGCAG GCGCACAAAG AGATCCCTCA AGGCTGACAC CCAGGAAGGG GCCTCTCACT TGTGGGACTC CCGAGATGCA 001200
001201 GTGGCCAACA CGAGTGTAGT GCCCAGTTCA CTGCTGGATA AGACAGAGGC CTGTTCTGGT CACACATGGG AGGCAGAACC 001280
001281 CAGAGACTGG GCCCAGGAGC CCTTCTGCTG ACAGTGGGAA CTCCCAGCTA CGTGTGGGGG TCCCCATACC AGACAAAGGT 001360
001361 CCCTGACCTT AGTCTTGCCC GAGAGGCCGA CACAGCCCAG CTTTGGGGTC TGGCTTTACC CACAAGAGGC CACACCTTGC 001440
001441 CACAGCACTG TTTATCTGGC CTGTTTCAGA AGCACCGTCA GAGTGGCGAA GGCAGGAGGT GGTGCACGAG AGTCTACGTT 001520
001521 CTAGCATCCA TTCAAGTGAG GGAAAGGCGT TTCGTACTTA GAAAAATGCA AAATTAATGA TTCTCACCCA CACATGAGAC 001600
001601 TGTGGATTCA CCCATGTATG AGATAGTGGA TTCAGAGCCA TGCTGTGGCT CTCATCTGCT GGGAGCCAGT AGTGTTTGTG 001680
001681 CTTTGGTTTT GCTTTTTTAG CTACATGGTT TATGTGAGGT GCGGACTTAC TTGAGTTGCT CTGTCTTTAC AGGGAGCTTG 001760
001761 CTTTTTCTCT GCTGGTTTGT TTTCACTTTT GCTGTCTCTG TTTAGAAGAA AACATTAGAA CTGGGGTGTG GTGGAAGGAT 001840
001841 GGGAGCACAA TCTGGGAGGG GAAGACCTGC TCTCTCTCCA CATACCAGGA GGGAGTGGCA GGAAGGGGAG ACACTGTGCC 001920
001921 GGACTCAGGG AGTCAAGAGC GTGAGCACCC CCAGGCTTCA ATCTGCTGAA CGTTGTGCAT CTGGGGGCGC CCGTGCAGCA 002000
002001 GGAACACTCT CCCCATCACA ATTCCTCTGG GCAC