NONHSAT084827

From LncRNAWiki
Revision as of 07:21, 22 November 2018 by Nashaiman (talk | contribs)
Jump to: navigation, search

TUG1 is a promising biomarker and/or a therapeutic target for bladder urothelial carcinoma.

Annotated Information

Name

TUG1: Taurine up-regulated 1 (HGNC nomenclature), also known as TI-227H; Linc00080; ncRNA00080 [1][2].

Characteristics

TUG1 is three exon transcript of 7542 bp (GenBank) long non-coding RNA that is found on human chromosome 22q12.2 (HGNC). Multiple Tug1 isoforms, with a main 6.7kb transcript in mouse. Spliced [1].

Function

Required for the proper formation of photoreceptors in the developing rodent retina [1]. Downregulation in developing retina leads to decreased retinal transcription factor expression and increased apoptosis [1]. TUG1 associates with PRC2 (polycomb repressive complex 2) and represses a number of cell cycle genes [3]. Depletion of TUG1 led to significant up-regulation of 120 genes, which were strongly enriched for those involved in cell-cycle regulation (regulation of mitosis, spindle formation, and cell-cycle phasing) [3].

TUG1-based signaling circuit in osteosar [4].

TUG1 promotes cells proliferation and inhibits cells apoptosis through regulating AURKA in Epithelial ovarian cancer (EOC) cells [5].

TUG1 functions as a sponge for miR‐219a‐5p to upregulate PIK3CA levels in osteosarcoma cells. In addition, the activation of the AKT pathway promoted TUG1 expression by up‐regulating the expression of Forkhead Box M1 (FOXM1), forming a positive feedback loop in osteosarcoma [4].

Expression

Expressed in the developing retina and brain [1]. Expressed in adult tissues with highest expression in cortex [1]. Expression levels are elevated in the caudate nucleus of patients suffering from the trinucleotide repeat neurodegenerative condition, Huntington's disease [6]. Localises to both the nucleus and cytoplasm [3]. Transcript was classified as unstable with a half-life >4 hr in human Hela cells [7]. Similar results were found in human B cells and mouse 3T3 cells [8][9].

TUG1 is overexpressed in bladder urothelial carcinoma compared to paired histologically normal urothelium [2].

Experiment Forward primer Reverse primer
Real Time Quantitative PCR 5'-TAGCAGTTCCCCAATCCTTG-3' 5'-CACAAATTCCCATCATTCCC-3'[2]


Conservation

Mammals - identified in mouse, rat, cow, dog and human [1]. Present in two locations in rat, with one an apparent processed pseudogene.

Misc

Nb: Mouse NRED accession is specific to one of two Tug1 isoforms.

Regulation

TUG1 is transcriptionally regulated by p53 in response to DNA damage [3].

Disease

  • B-cell neoplasms
  • Bladder urothelial cancer [2]
  • Epithelial ovarian cancer [5]
  • Huntington's disease [6]
  • Non-small cell lung cancer
  • Osteosarcoma [4]

Labs working on this lncRNA

  • Department of Imaging, People's Hospital of Lishui City, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China. [5]
  • Department of Gynaecology and Obstetrics, People's Hospital of Lishui City, the Sixth Affiliated Hospital of Wenzhou Medical University, 15 Dazhong Street, Liandu District, Lishui 323000, China.[5]
  • Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, China.[2]
  • Institute of Urology, Shenzhen PKU-HKUST Medical Center, Shenzhen, China.[2]
  • Central Laboratory, Shenzhen Second People’s Hospital, Shenzhen, China.[2]
  • Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China.[4]

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Young T L, Matsuda T, Cepko C L. The noncoding RNA taurine upregulated gene 1 is required for differentiation of the murine retina[J]. Current biology, 2005, 15(6): 501-512.
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Han Y, Liu Y, Gui Y, Cai Z. Long intergenic non‐coding RNA TUG1 is overexpressed in urothelial carcinoma of the bladder[J]. Journal of surgical oncology. 2013, 107(5):555-559.
  3. ↑ 3.0 3.1 3.2 3.3 Khalil AM, Guttman M, Huarte M, Garber M, Raj A, Morales DR et al. Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression[J]. Proceedings of the National Academy of Sciences. 2009, 106(28):11667-11672.
  4. ↑ 4.0 4.1 4.2 4.3 Li Y, Zhang T, Zhang Y, Zhao X, & Wang W. Targeting the FOXM1‐regulated long noncoding RNA TUG1 in osteosarcoma[J]. Cancer science. 2018, 109(10):3093.
  5. ↑ 5.0 5.1 5.2 5.3 Li T, Chen Y, Zhang J & Liu S. LncRNA TUG1 promotes cells proliferation and inhibits cells apoptosis through regulating AURKA in epithelial ovarian cancer cells[J]. Medicine. 2018, 97(36):e12131.
  6. ↑ 6.0 6.1 Johnson R. Long non-coding RNAs in Huntington's disease neurodegeneration[J]. Neurobiology of disease. 2012, 46(2): 245-254.
  7. ↑ Tani H, Mizutani R, Salam KA, Tano K, Ijiri K, Wakamatsu A et al. Genome-wide determination of RNA stability reveals hundreds of short-lived non-coding transcripts in mammals[J]. Genome research. 2012: gr.130559.111.
  8. ↑ Friedel CC, Dölken L, Ruzsics Z, Koszinowski UH, & Zimmer R. Conserved principles of mammalian transcriptional regulation revealed by RNA half-life[J]. Nucleic acids research. 2009, 37(17):e115-e115.
  9. ↑ Clark MB, Johnston RL, Inostroza-Ponta M, Fox AH, Fortini E, Moscato P et al. Genome-wide analysis of long noncoding RNA stability[J]. Genome research. 2012.

Sequence

>gi|55000|ref|NR_002323.2| Homo sapiens taurine up-regulated 1 (TUG1), transcript variant 3, long non-coding RNA

000001 TCCTGCTTTC CTGACCCTCT CCGCCATTTA AAGAAACAGT ACCGGGGGCG GGCCGAGCGA CGCAGCCGGG ACGGTAGCTG 000080
000081 CGGTGCGGAC CGGAGGAGCC ATCTTGTCTC GTCGCCGGGG AGTCAGGCCC CTAAATCGAA GAAGCCCTGG CGCGCCCTCC 000160
000161 CCCCCTCCCG GGTCTGGTAG GGCGAAGGAA CGGGCGTGCG GTCGATCGAG CGATCGGTTG GCGGCTCTTT CTCCTGCTCT 000240
000241 GGCATCCAGC TCTTGGGGCG CAGGCCCGGC CGCCGCGGCG CGCGCCCGGT GGCCGTTGGC GCTCGCGCCG CGTCTTTCTT 000320
000321 CTCGTACGCA GAACTCGGGC GGCGGCCTAT GCGTTTGCGA TTCGACGAGG AGTCGTCCGG GTGGTCGGCG GCGGCGGGCA 000400
000401 GCTGCTCCGC CCCGCTCCGG GGGAGGCGGC GGCGGCAGCG GCCGCGGGAT TTGGAGCGGC CGGGGAGGCG GGGGTGGCCG 000480
000481 GGGCCGGCTT GGAGGCCTGG CGCCACCCTT CGGGGCCTGC AAGGACCCAG TTGGGGGGGC AGGAGGGGGC CGGAGGATGG 000560
000561 TTGGTTGTGG GATTTCTACT TTGCCTTTTC CTCCTTATGC CGCCTTAGTG AGGGGCGGGA GCTCTGGCGG CAGCCCCGGG 000640
000641 GTGGGGAGAC GAGCTCCGGA GTCGGAAGAG CTGGGTTTTC TTCCGGGCCT AGCCACCAGT TGGCGGAGTG ACCTTAGGCG 000720
000721 AGTCACTCTG TAATTTGTCT GCGCCTCAGT TTCCTCCTCT GCCTATCAAT GTGTGTGGGG TTGAAATCGC TTTGTAAACT 000800
000801 ATAAAGCGTG GGTGTACGTA AAGGATGGTT ATTGTTTATA ATTTTTTTTG AGTTGTAAGA AAACTTAGCA GTTCCCCAAT 000880
000881 CCTTGGGTTT TGAACCTGGG AACCTTGGAT TGGAGTTGGG GATCCCCAAA CTTCCTGAAA TTGTGGGAAT GTGCGGTTTG 000960
000961 GGGGAATGAT GGGAATTTGT GGGAATGTGC GTTTTAGGGG AATGATGATC CATCGCTAGC AAGTTTTCCA AGGGGGCTGT 001040
001041 GACCCAGAAG AGTTAAGAAT CACAATTTCT TCATGCTACA GAGAGGAAAC TGAGGCCTAG ATGTCATTTG GGACCCTTCA 001120
001121 CAACCATTTT GAAGCCCTGT TTGAGTCCCT GGGATATGTG AGCTGTTTCT ATGCATAATG GATATTCGGG GTTAACAACA 001200
001201 GTCCCCTGCT TGGCTTCTAT TCTGAATCCT TTTCTTTCAC CATGGGGTGC CTGAAGGGTG GCTGATGCAT ATGGTACAAT 001280
001281 GGCACCCAGT GTAAAGCAGC TACAATTAGG AGTGGATGTG TTCTGTAGCA TCCTATTTAA ATAAGCCTAT TTTATCCTTT 001360
001361 GGCCCGTCAA CTCTGTTATC TGCTGCTTGT ACTGGTGCCT GTACTTTTCT GACTCTCATT GACCATATTC CACGACCATG 001440
001441 GTTGTCATCC ATTACTTGAT CCTACTTTAC ATGTCTAGGC TGTGTGGTTG GTGGTGAATA GGCTTCTTTT TACATGGTGC 001520
001521 TGCCAGCCCA GCTAATTAAT GGTGCACGTG GACTTTTAGC AAGCGGGCTC ACTGGAAGAG ACTGAACCTG GCATGGAATT 001600
001601 CCTGAAGATG TTTGGGGTTT TTTTCTTTCT TAATCGAAAG TTAACATTGT CTGAAAAGTT TTGTTAGAAC TACTGCGGAA 001680
001681 CCTCAAAATC AGTAGATTTG GAAGTGATTC AAAGCTAAAC TTTTTCCTTG GCCCTCCTTG TGTTCTAATT GCTTGCAAGT 001760
001761 GTAATACTAG GATGTCCAAG ATGCCAGTTT TTGCTTCTTT GTTAGTTGTC AGCTGCTTTT ATCAAATTTC AGGCCATTAT 001840
001841 CCAACAAACA CTATAAAAAT GTTTGAACAA TTGGATTTCA AACATTTTCG TTTTGTGGAG TGGTGCTCAC CAAGTGGTAC 001920
001921 AGCCCTAAGC AAGTGAACAC AAACACATTT AAGTGTATTT TGTCTGATTA GATGTTAGCC AGTTATGCTA TTTCATTCAA 002000
002001 ATGTCTGAAA AAATCAATTG ACTATTCCCT TTTCCTAAAG GGCAGAGACA GATAATCTCA CTTCCAGAGA AATGACTTGG 002080
002081 AGAAAAAAAA GTGTTGGTCT TTTTGCTCTT TTGTAATTAA ATCCGGATGT ACCTCAAAAG ACTTAAGACT GTGGTGATAA 002160
002161 GATGCTTTCC TCAGCAGAAA GGAGGGAAAA AAAACAACTG GAACTCAAAG CTTGAAATTC TGTGGCAAAA CATGAGATGT 002240
002241 CCAGGATTGG AGGTTGAAAA GATTTCACTA CAGTGTTCTG CAATAGTTGG AGCAGATAAC TTTCAGTGTA GCCACAGCCA 002320
002321 TGGACTCCAG ATTTCCAGAT TTTCAAGACC TGGACCTGGA ACCCGAAAGA GCTTGTCACG ATGCGGCAGG AACACTGGAG 002400
002401 GTAGATTTTT TTTTATTTTT GAATTTTGGG ACTGTTGACC TTGCTGTGAG AAAAGAGACA ACGACTGAGC AAGCACTACC 002480
002481 ACCAGCACTG TTACTGGGAA TTAGAAGACC TGAGTTTCTG TCCAGACCCT CAGTGCAAAC TGAGGATGCT CCATCCAAAG 002560
002561 TGAATTATGT CCTGTGCCTC CTGATTGCTG AGTGTTCACC TGGACCTTCT GACTACCTTC CCTGTGCTAT TCCATCAGCC 002640
002641 TACAGACCTG GTACCTGGAT TTTTGCCCGA GATGATTCCT ACCACCTTAC TACTGACGAA GACACCCATT CCAGTGGACC 002720
002721 ACTGTGACCC AGGAGGCATT CAGCCATCAT GATGTGGCCT TTACCTCCAC TCCTGTCTTG TTCTACCCAG ATTCAGCACA 002800
002801 GCCCTTTATA GTGAAGTCAG AGTCCTCAAG CCAAATAGCT AAAGCTGTTT TATCACAACA AAGGCCTAGT TTGTTCCATG 002880
002881 AGTGTGCATT TCATTTCTTC AGTTAAAGCC TTCAGAGACA CACAATAAAT TTGGACCAGG GGATTTTTTA GTTATTAATG 002960
002961 CTCTCTGAAG AAAGGCAACA TCTTTTTGAG AGCAGCATTG GACCACACCC CACAATCTCA AATGATTGAA ATTCATGAAC 003040
003041 ATCTAGGATC CCGTGAAGGT CACTGGACCC TGTTTTTTCT ACTTCAAATC CTGTAGTAGC CTACTGAATG AGAAAACATA 003120
003121 TTCTGACCCA TTGGGATCAA ATCAAAGGCA CAGTGAACTC CTCATAGCAT CTTCTTTGGA ATTACTCAGG AACCAGAACT 003200
003201 TTTTACACAA ATGTAAGAAA TTCTACCAAG GAGTCCCCTT ACCTAACAGC ATCTCACAAG GCTGCACCAG ATTCCAGAAA 003280
003281 AGGCTTCTCT TGATACATCA AGGTAGAACC TCTATGCATT TTGTGACCGA CTTATTCTTA GATCATTGGT TTTCCAAAGG 003360
003361 CTTTGTGGCC ATGAAGCCCT TTGAGTGAAA ACTGTGCAGA AGCCCAGAGT AAAAGTGAAG CTGCTCTGGA TGAAGTAGTG 003440
003441 AAGCAAGAGT AGGGGCCTGA ATCCTGCTAC AACTATCTTC CTTTACCACC GTGGTGACAC CTAAGGGGAC TTCCTTACAA 003520
003521 CACCTTGAAC TCTTCCGAAC ACAGTTTGAA AACCACTGCC CCAGACAGCA ATATGTTTGA CCTGAATGGC ATTCCAATCT 003600
003601 TTTCTGTACC TCCACTCAGC ACAGTTCATG TTCAGTAGAT GCTGAACATT CTTAGAAATA CTGTGTGTGA ACTTAGAAAA 003680
003681 GTGCAAGAAG ACAGGCATGT CTTTGACCCC AGGAATGATC ATTTGCTGAA GATGGTGTCA AGTGAACCTA GATTAACAGC 003760
003761 CCTCCACTCC AGATGGATAT CCAGTGATTC CTAGAATGGG ATATAGCCAG AGAACAATTC TATGCACCCT ACACTGACAG 003840
003841 ACTCCCTTAA GCAACACCAG ATGCTCTACT GGTACTTGAA GTACATGACT TTGAAGTCTT GACCCTCCAT GAATACCTGA 003920
003921 ATTATCAGCA AGCGGGTTTT GAAGCTGGTG CCTCATTGAG GCCATATTAG AGCAACTTGT ACATTTGACC TCTTGTTATC 004000
004001 AGCCATGGTA CTCTACTTCG TGTGCAAGAG ATAACTATGA AAGCCAAATT CAAATACTGG CAACATTTCC TAAAGGGGCT 004080
004081 CAATATCTAT CATTCGTCTT CTTTTCCAAA CTACACATCA CTGTATGACT CAACCAGTAG CAGTTATATT GCCCCTTGGT 004160
004161 TTTTATTCAG TTTAACTACT GTTTCCAAGA TAAATGAGCT AATAAGCTTT AAAAAAAAAA AAAAAAAAGG CTGAATTCTT 004240
004241 TTTTCTTCAT CACTGGCATA TCTGCCTATT CTCCAGAATT ATTATGACTA TTCAGCTCAC TTTAACAGTT GAACTTCAAG 004320
004321 CGACAATCTT TGAACACCCC TTCTCATGTG ATTTAAAATG AAACCATTTG GAAAAGTTTC TTCTAGCCAG TAATAGATTT 004400
004401 TTTTTTTAAT TGCTCTGCCT TGTGCCGAGA GATGTTCTTT TAAGATGAAT CTTTTGATGT CTGATACCAC CAAATATAGG 004480
004481 TGGTAGGGAG AGTTGGAGGC TGGCCCTTTG AGCAGGCCAT TAGCTTACTT GCTGGGCATT TCCGATAGCT TATTGCCTAC 004560
004561 CTTTTTGCTG GAAACAAACT GATTTGAAAA ACAAAATCTA TGAAGACTGC AGCTAAGGAT TTTATCGGTA GACTTAAGAG 004640
004641 CTTTTGTCCT TGTGGATATT TTAGTGGAAC CACATCAGTC TCAATACTGT CATTTTACAC TGACTCAGAG CAGCTGACTT 004720
004721 CATTCCTTGC CATGATATAT ATTTAAGGCA GGCATTGTAA CAGACATAAA GACAACTTAT CTGTTTCAGC AGGAAGGATT 004800
004801 CAGTTTATGA ACTCTCAGAC CAGATCATGT TGAACAAGGA GACTTTGATG TGTGTCATGA GAAAACTCAT TCTTTACTTC 004880
004881 CCAGTCAATT TAAAGGCCAG CTATCCTGAG CTACTCGAAT GAATGCACTG GTTAAACATT GGAAATAGTT TGTTTATATC 004960
004961 CTTGTCTCTC TCTAGGCCAA TTGTGATTAC ATGACTCGAC TCTACATCTC GTCAAACAAG GCCTAGGTCT GGTTGCTGTA 005040
005041 GACTGCTCGC CCTCAACAAA TAAAATCTGG TTGACTAGCC TCCTTGTATA TACAACTATT ATTTGTTAAG AAGAAATTAT 005120
005121 CGTCAATTTT CTACTACCTT CCAATTGTCA GCTCTTTTTT TCCTCTCTGG TTTTTCCTAT ACTTTACAGA AAAAGACATT 005200
005201 GATCTATACT GCCATTCCCT CTAATCCTGC CATACTCAGT CAAAAGGAAT GACTTAAGAT GAAGATGATC ATCTGCTCGA 005280
005281 GTCTAAAATA TACATTGTAT ATAAGAATTG GTGATTAGAA AAGCAAAAAA CCTAAAACTT AAATCTAGGA GTCTGTATAC 005360
005361 TGTCTCCATG TCTCCATGCC TCAGATCTCA TCTAAATCTT TGAACAGCAC CATTCAACCA ATCTGAGGCC TTGACTTGCT 005440
005441 TGTAAGATGA TTCTCAGAGA TCGGCTGAGT TAAAAAAGAT GACGACTTGA TTACCAAAGA AAGTAGGGCC AACTTTGACA 005520
005521 AATCTGGCTC TGCTGACCCT GTCACTCCCA GATGTAGCAT AGACTCCTAA ACAGAACCTC AAGTCTGATT GAGGATAAGG 005600
005601 CCTTCTCCTG AGCTGAAAGT TCTTTGGCAG ATGAGCAAGA AACTGAAAGC TGATGTACCT GACTGGCTCT GTAAGATCAG 005680
005681 AAAACTGTAT CCAGAATAAG CCCTATGGAT TAACCCCTGA GTACCCAGAG TAAAAACTAA TTTACAGAAC TTCCTTATTG 005760
005761 ATCTGCTGGT TCTTCCAGAT CATATTCTGG CTATTGGTAT GGCTGGCCTT TCTGAAGGTA CCCTGCTTGT CTATTTTCCT 005840
005841 GACTCAGCTC TTGCCTGCCT TTTTCACATG TTGCTGCAAT TAGACTCACC GTGAGGACTA CAGTCAATTT CAGTCTATCT 005920
005921 TGTGCCCAAT ACAACAAGGA TTTTTAATAG TAACAACCCA CACCTCACCC ACTAGGACTC AATGTTCACA ACAGGAAGGA 006000
006001 CCATTGCTGC ATACTCCTTG ACCAGCAACT TTTTTGAAGA TATTTTTAAG TGCAGAGTAG GCCTCTATTC CTGTATGTAA 006080
006081 TTGTTCATTT TCAGCACCTG GAACCTCATC TATCGGGTCT GGAAGGAATA CAGCAGTTCG AAAGCCGCGT CCATTTCTCT 006160
006161 CCTTCAGTAG TGCAGAAATG AGTCCGATTC ACCAGTACAC ACAGAACTGT ACCAGTTCAA CCTAGCAAAA GAAGAAAAGT 006240
006241 TTCCACTGTA CTTAAAATTT ACAGCTGACT CAAATTGCCT CACAGAATTA TTTGATGTAG AAGGCTAGTT GTCTTACTTC 006320
006321 AGATCAGCAG GACAGTTGGG CTCTCAGACT CATGACCACT GAGTTTGCTT GTGTTGAAAC TGTGGTTTCA TCCAACATAT 006400
006401 GCTATTGGAC ATGATTATTA TTCCATTCAA ATGGATTACA GACTTCTTGA GGACAGGACA AACTTATCTC TCATGGTGTT 006480
006481 TTTTTAGAAT ACTTTTATAA CCAAGGAAGA AACCATGCCA GCTGTTACCA TTCAACTTCT TAAGCAGAGA TTAAGCTTTT 006560
006561 TCATATCTGT TCTTATCCTG GACATCAGTA GTTTTTAATT GCCCAGCATC CGTTCCATCT TGTAACAACT CCCTGATGTT 006640
006641 TCTTAAAACC ACCTCTTCCT ATTTTCAGTC TGTGGTTTGG ACAGTCTGAC CCAACCTTGA GCTTTGTGGG TGAACATGTA 006720
006721 ATTCAGACCT CATCAATCAG CAAATCCATC TGAACTGTGG AGGAGA