File:Expression 21.png
Genome-editing integrates XIST into Chr21 in trisomic iPSCs a. Concept for translating dosage compensation to trisomy 21. b. XIST construct (19kb): two homologous arms and 14kb XIST cDNA with inducible pTRE3G promoter. c. DNA/RNA FISH in interphase DS iPSCs shows XIST overlaps one of three DYRK1A genes in non-expressing cell (top, arrows), and a large XIST RNA territory with DYRK1A after 3 days in dox (bottom, arrows). Scale: 2 μm. d. OCT4 immunostaining & XIST RNA FISH in a transgenic colony: highly consistent XIST expression throughout the colony. Scale: 100 μm. e. Metaphase DNA FISH shows one targeted Chr21. XIST gene (asterisk and close-up)overlaps one of three DYRK1A genes (arrows).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 06:59, 14 October 2014 | | 566 × 772 (425 KB) | Linxia (talk | contribs) | Genome-editing integrates XIST into Chr21 in trisomic iPSCs a. Concept for translating dosage compensation to trisomy 21. b. XIST construct (19kb): two homologous arms and 14kb XIST cDNA with inducible pTRE3G promoter. c. DNA/RNA FISH in interphase DS ... |
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