CASC8
CASC8：cancer susceptibility candidate 8,located in 8q24.21 region, is suggested with cancer, such as colorectal cancer, prostate cancer, intestinal tumor，[1] gastric cancer[2]
Contents
Annotated Information
Name
CASC8:cancer susceptibility candidate 8(non-protein coding)
CARLo-1,LINC008600,”long intergenic non-protein coding RNA 860” [1]
Characteristics
Seven lncRNAs are located in the 8q24.21 gene desert. The region including CARLo-1∼6 [1]
Function
Inhibition of CARLo-5 dramatically reduced the number of cells and colonies in vitro, indicating a role of CARLo-5 in cell transformation. CARLo-5 is not only overexpressed in tumors but also involved in tumorigenesis. [1] CASC8 may presumably play a key role in modulating the recruitment of general transcription factors on the promoter of its cognate gene, POU5F1B, which was found to be a putative cancer susceptibility gene. It is biologically plausible that allele of rs10505477 further alters the fine turning interactions between the CASC8 and POU5F1B. [2].
Regulation
CASC8 regulated by rs10505477 ,which was involved in clinical outcomes of gastric cancer, act as a cancer susceptibility candidate. [2]
Diseases
Expression
CARLo-5 is overexpressed in CRC and involved in oncogenic transformation and tumor incidence.[1]
| Target lncRNA | Use | Sequence |
|---|---|---|
| CARLo-1 v1&3 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3′ nested PCR and sequencing |
GGCAGGTTGTGTGTCACGCCCTCCACT
ATGTTTCATTGGCTGAAGCTCAATTG CCTCAGGAACACACATGGATGATTCT AAAGAGTATGGAGGCTTGAACATGGC AGTGGAGGGCGTGACACACAACCTGCC GCCATGTTCAAGCCTCCATACTCTTT[1] |
| CARLo-1 v2&4 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3’nested PCR and sequencing |
GCTCCTGGTCCATGCCATTCATCAATTG
GCTGAGGACAGAAGGCATTGGTCT GAGCATCCGAATAAATAAACATACAAATGC ATTTTTCACCTGCTTCAGTGCCATTGT GAGACCAATGCCTTCTGTCCTCAGC AATTGATGAATGGCATGGACCAGG[1] |
| CARLo-2 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3′ nested PCR and sequencing |
CCTTGACTTGAGCCCAGGTCACCATGC
ACGTCTTCTCTTGGCTCCTCTGGAT AAACTATTGATGCAGTGTCCAAGGTGG ACATCAGTTAAAAGGCCAACAGGAACC GCATGGTGACCTGGGCTCAAGTCAAGG CTTATTTCTCTGCTGAACCATCACA[1] |
| CARLo-3 | CARLo-3 | TGGGCCTAGTACTTGGAGACGGGGACTG
TTGGATGATAGTGAAGGTGAACGTCTCT AACGAAGAGCATCACCATTTCCACCA ACTCTCCTAATGGGCCTAGTACTTGGAGAC AACGAAGAGCATCACCATTTCCACCACC AGAGACGTTCACCTTCACTATCATCCAA[1] |
| CARLo-4 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3′ nested PCR and sequencing |
GTGACAGACGGAGTGCTGATGCCTCTGG
AATCTCTTCACATTGTGGAGTTTCTCCTC ATCTAGCAAATAGGCATCTGTCACAGAAAATAGA ATCAGCCTAACCAGCACGCATAAACTAAAC CCAGAGGCATCAGCACTCCGTCTGTCAC TCTGTCACTATTGGCATCCTGTGACA[1] |
| CARLo-5 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3′ nested PCR and sequencing |
GGGTTGAGAAGGGGTGAAGGGACACTGG
AAGTGAAGCTGGGAAGTTTGCACTTG GCCACAAATCAACAACAACAACAACAA AGAGTGATGCCAAGGCTGTTATTGTCAA CCAGTGTCCCTTCACCCCTTCTCAACCC[1] |
| CARLo-6 | 5′ RACE
5′ nested PCR and sequencing Internal-F and sequencing Internal-R and sequencing 3′ RACE 3′ nested PCR and sequencing |
GGGTGCCATCACAGTCAGTTTGGACAGC
CTTGAATGCTATCAGGGAAGCTAGAGTG CTTAGAATTGGAGTGCCTGGGTTAGAA GCAGTCATTCAGGAATTGAAGCTGATA GCTGTCCAAACTGACTGTGATGGCACCC AGACAGACAGCGATGCCACATGTT[1] |
Sequence
>gi|211971085|ref|NR_024393.1| Homo sapiens cancer susceptibility candidate 8 (non-protein coding) (CASC8), transcript variant 2, long non-coding RNA
000081 TACTTCCTTC AGGTTTCACT CAGAAATGAC TTTCTCAGTG GGGTCTCCCA TGACCATTTT ATTTAAGATT TCAACTACTT 000160
000161 TGCCAGTATT TCACTTCTCC TCCCTTGATC TTTTCTTCCA AGCACTCACT GCCCTCTTGC ACACTGTATC TTTTACTTAT 000240
000241 TTATCTTGTT CAGTTTCTGC CTGCCTCACC TGCCTCACCA CAGTGTACAT TTCACGAGGG CAGGGATTTT GTCTGTTTTG 000320
000321 TTGATTGCTG TAGCCCTGGC ATCTTGGGCA GTGCCAGCAC CTGTTAGCAT TAAATACATA TTTACTGAAT GAATGAGTGG 000400
000401 CTTCATGCTT AAAAGCAAGA AGAGAGACCT TCATAGAGAA GTAGGAAAAT GAATTGTTGC ACAAATAAAG GGCCATGTAA 000480
000481 TGAGAGAGAG AGAGCAAGAG AAAGAGCAAA CAATTAAGGA GCAGCAGTGG CTTCCTGCAG GGGAGACCAA TGCCTTCTGT 000560
000561 CCTCAGCGGA AACAGCTTGG CATTCTTCCA ATTGATGAAT GGCATGGACC AGGAGCACTA GTTATCTTCA GCTTCTGAGC 000640
000641 ATCCGAATAA ATAAACATAC AAATGCATAA CATAAAATAA ATTCAAATTA CAAATGAGAA AACCAATCTA GGTTACCGGC 000720
000721 AAGTCCTATG ACTTGATGTG GAGGCCTGGA AGTGCATCCT TGAATCTTGC AATGCAGTGA GCCAAGGAGC AATGAGAGGC 000800
000801 CACATGAATA ACCGGGCCAG TGACAAGACA CATGTCCTCC TCCTTGCTGG TATACACCAG AATGCCCCGC ATGCAGCGAA 000880
000881 TGCTCAAAAA GTGTTGGTGG AAGTGACAGA ATTCCATCAC CATTAAACAC AACCAGTGTT GCGGTTGACA ATGGCACTGA 000960
000961 AGCAGGTGAA AAATCCGTAC AGCAGCTTCT GTCTTTGAAG AGTTTGCAGC CTCTTGAAGA GGGTTTAGAG GTTAAAAAAA 001040
001041 GGACCCAGGC TGATAGGGCC GCTACCATTT GGAAAGTGAC AAGAGGAAGA GAGAGAATGA AACAACAGTA AGCACTGGCT 001120
001121 CTCAGCTTCT ACCTGAAGAG ACAAACATTG CTTTCACTTA CATTTCACTG GCCAAATCAA GCCCATGGCA ACAATTGAGT 001200
001201 TCAAAAGCAT AAGGAGGTAT CTGGAAGGAA GAGAATCAGA AAATTTCTGA TGAGCCTCAC TGACTACCTT CAGGGCAAAA 001280
001281 AGGAAGAGAG GCACTCCAGA TCAGAGATCT GCATTGGAAA CTCCTTGAGA ACCAGAAGGG TATGCTGTAT TTTTGTAACT 001360
001361 GTGAGGAGCC CAGAAAGACT GAAGCCCAAA TAAAATTCCT TTATTCAATA TT
Labs working on this lncRNA
- Department of Molecular Virology, Immunology and Medical Genetics andDepartment of Anesthesiology, Wexner Medical Center, and Department of Neurological Surgery, The Ohio State University, Columbus, OH, 43210; [1].
References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Kim T, Cui R, Jeon YJ, Lee JH, Lee JH, Sim H, et al. Long-range interaction and correlation between MYC enhancer and oncogenic long noncoding RNA CARLo-5[J]. Proceedings of the National Academy of Sciences of the United States of America. 2014,111(11):4173-8
- ↑ 2.0 2.1 2.2 2.3 Ma G, Gu D, Lv C, Chu H, Xu Z, Tong N, et al. Genetic variant in 8q24 is associated with prognosis for gastric cancer in a Chinese population[J]. Journal of gastroenterology and hepatology.
