File:Antisense noncoding RNA ANRASSF1 is expressed within the RASSF1 genomic locus and inversely correlated with RASSF1 expression.jpg

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Image title	Antisense noncoding RNA ANRASSF1 is expressed within the RASSF1 genomic locus and inversely correlated with RASSF1 expression.(A) Schematic representation of the entire RASSF1 locus presented at the top. Protein-coding RASSF1 gene isoforms (RefSeq-annotated) are shown in black; ANRASSF1 evidence from an assembly of public ESTs is shown in light gray; the portions extended through 5?-end RACE and primer-walking or 3?-end RACE are shown in red, with a poly(A) segment detected through sequencing. The arrowheads define the orientation of the sequences. (B) ANRASSF1 was detected in several human cell lines using end-point PCR in the antisense (AS), and not the sense (S), direction relative to the protein-coding gene. Strand-specific primers were used for the reverse-transcription (RT) (short half-arrow numbers 2 and 3 on ANRASSF1 in panel A). RT reaction in the absence of primer was used as a negative control (Ctl). The short half-arrows numbered 1 and 4 indicate the primers used for the end-point PCR. (C) Strand-specific RNA-seq from poly(A)+ RNA of LNCaP cells. The abundance of the reads mapping within the RASSF1 genomic locus is displayed in the diagram, and the color corresponds to the DNA strand of the transcripts. The red horizontal bar represents the consensus sequence from the assembly of RNA-seq reads mapping to the positive strand in this locus, generated using the Cufflinks tool. The gray bar represents the full-length ANRASSF1, obtained by RACE-PCR and Sanger sequencing. The arrowheads define the orientation of the sequences. (D) Expression of RASSF1 and ANRASSF1 measured with Affymetrix microarrays in HeLa, MDA-MB-231 and MCF-7 cells from GSE5823 [54]. (E) The expression of RASSF1 and ANRASSF1 measured with Affymetrix microarrays in Jurkat cells under mitotic stress induced through treatment with phorbol ester and ionomycin for 30 and 60 min from GSE11118 [55].
Copyright holder	Creative Commons Attribution License
Date(s)	22 August 2013
Publisher	Public Library of Science
Short title	Figure 1
Source	info:doi/10.1371/journal.pgen.1003705
Identifier	info:doi/info:doi/10.1371/journal.pgen.1003705.g001
IIM version	2